scholarly journals SLL1-ZH Regulates Spikelets Architecture and Grain Yield in Rice

Agriculture ◽  
2021 ◽  
Vol 11 (11) ◽  
pp. 1162
Author(s):  
Lianping Sun ◽  
Jingxin Wang ◽  
Xiaoxia Wen ◽  
Zequn Peng ◽  
Daibo Chen ◽  
...  
Keyword(s):  
Grain Yield ◽  
Morphological Analysis ◽  
Floral Organ ◽  
Rice Grain ◽  
Related Factors ◽  
Single Nucleotide ◽  
Floral Organs ◽  
Rice Mutant ◽  
Organ Identity ◽  
Yield Formation

The spikelet developmental processes that control structure and floral organ identity play critical roles in rice grain yield formation. In this study, we characterized a novel rice mutant, SLL1-ZH, which exhibits a variety of defective agronomic characters, including semi-dwarf, rolling leaf, deformed panicles, and reduced grains production. Morphological analysis also revealed that the SLL1-ZH mutant shows numerous defects of floral organs, such as cracked glumes, hooked and thin lemmas, shrunken but thickened paleas, an indeterminate number of stamens and stigmas, and heterotopic ovaries. Map-based cloning identified a single nucleotide substitution (C to G) in the first exon of LOC_Os09g23200 that is responsible for the SLL1-ZH phenotype. In addition, qPCR analysis showed a significant change in the relative expression of SLL1-ZH in the mutant during inflorescence differentiation and in the different floral organs. Transcription of rice floral organ development-related factors also changed significantly in the mutant. Therefore, our results suggested that SLL1-ZH plays a great role in plant growth, spikelet development, and grain yield in rice.

scholarly journals DEGENERATED LEMMA (DEL) Is a New Allele of OsRDR6 That Regulates Lemma Development and Affects Rice Grain Yield

2022 ◽  
Author(s):  
Jing You ◽  
Qiannan Duan ◽  
Jun Zhang ◽  
Wenqiang Shen ◽  
Yue Zhou ◽  
...  
Keyword(s):  
Grain Yield ◽  
Nucleotide Substitution ◽  
Floral Organ ◽  
Rice Grain ◽  
Amino Acid Mutation ◽  
Single Nucleotide ◽  
Organ Identity ◽  
Floral Organ Identity Genes ◽  
Seed Setting Rate ◽  
1000 Grain Weight

Abstract The lemma and palea are floral organ structures unique to grasses, and their development affects grain size. However, information on the molecular mechanism of lemma development is limited. In this study, we investigated a rice spikelet mutant, degenerated lemma (del), which developed florets with a slightly degenerated or rod-like lemma. The results indicate that the mutation of the DEL gene interfered with lemma development. In addition, del also showed a significant reduction in grain length and width, seed setting rate, and 1000-grain weight, which led to a reduction in yield. The results indicate that the mutation of the DEL gene further affects rice grain yield. Map-based cloning shows a single-nucleotide substitution from T to A within Os01g0527600/DEL, causing an amino acid mutation of Leu-34 to His-34 in the del mutant. DEL is an allele of OsRDR6, encoding the RNA-dependent RNA polymerase 6, and is highly expressed in the spikelet. RT-qPCR results show that the expression of some floral organ identity genes was changed, which indicates that the DEL gene regulates lemma development by modulating the expression of these genes. The present results suggest that DEL plays an important role in lemma development and rice grain yield.

scholarly journals Pitfall Flower Development and Organ Identity of Ceropegia sandersonii (Apocynaceae-Asclepiadoideae)

Plants ◽  
2020 ◽  
Vol 9 (12) ◽  
pp. 1767
Author(s):  
Annemarie Heiduk ◽  
Dewi Pramanik ◽  
Marlies Spaans ◽  
Loes Gast ◽  
Nemi Dorst ◽  
...  
Keyword(s):  
Gene Expression ◽  
Floral Anatomy ◽  
Floral Organ ◽  
Mads Box ◽  
Floral Organs ◽  
Abcde Model ◽  
Organ Identity ◽  
Mads Box Gene ◽  
Flower Evolution ◽  
Developmental Phases

Deceptive Ceropegia pitfall flowers are an outstanding example of synorganized morphological complexity. Floral organs functionally synergise to trap fly-pollinators inside the fused corolla. Successful pollination requires precise positioning of flies headfirst into cavities at the gynostegium. These cavities are formed by the corona, a specialized organ of corolline and/or staminal origin. The interplay of floral organs to achieve pollination is well studied but their evolutionary origin is still unclear. We aimed to obtain more insight in the homology of the corona and therefore investigated floral anatomy, ontogeny, vascularization, and differential MADS-box gene expression in Ceropegia sandersonii using X-ray microtomography, Light and Scanning Electronic Microscopy, and RT-PCR. During 10 defined developmental phases, the corona appears in phase 7 at the base of the stamens and was not found to be vascularized. A floral reference transcriptome was generated and 14 MADS-box gene homologs, representing all major MADS-box gene classes, were identified. B- and C-class gene expression was found in mature coronas. Our results indicate staminal origin of the corona, and we propose a first ABCDE-model for floral organ identity in Ceropegia to lay the foundation for a better understanding of the molecular background of pitfall flower evolution in Apocynaceae.

The Arabidopsis nectary is an ABC-independent floral structure

Development ◽  
2001 ◽  
Vol 128 (22) ◽  
pp. 4657-4667 ◽  
Author(s):  
Stuart F. Baum ◽  
Yuval Eshed ◽  
John L. Bowman
Keyword(s):  
Ectopic Expression ◽  
Floral Organ ◽  
Floral Structure ◽  
Floral Organ Identity ◽  
Floral Organs ◽  
The Third ◽  
Multiple Factors ◽  
Organ Identity ◽  
Selector Genes ◽  
B Class Genes

In contrast to the conservation of floral organ order in angiosperm flowers, nectary glands can be found in various floral and extrafloral positions. Since in Arabidopsis, the nectary develops only at the base of stamens, its specification was assayed with regard to the floral homeotic ABC selector genes. We show that the nectary can form independently of any floral organ identity gene but is restricted to the ‘third whorl’ domain in the flower. This domain is, in part, specified redundantly by LEAFY and UNUSUAL FLORAL ORGANS. Even though nectary glands arise from cells previously expressing the B class genes, their proper development requires the down-regulation of B class gene activity. While CRABS CLAW is essential for nectary gland formation, its ectopic expression is not sufficient to induce ectopic nectary formation. We show that in Arabidopsis multiple factors act to restrict the nectary to the flower, and surprisingly, some of these factors are LEAFY and UNUSUAL FLORAL ORGANS.

scholarly journals Arabidopsis QWRF1 and QWRF2 Redundantly Modulate Cortical Microtubule Arrangement in Floral Organ Growth and Fertility

2021 ◽  
Vol 9 ◽  
Author(s):  
Huifang Ma ◽  
Liyuan Xu ◽  
Ying Fu ◽  
Lei Zhu
Keyword(s):  
Cell Expansion ◽  
Expression Patterns ◽  
Cortical Microtubule ◽  
Floral Organ ◽  
Organ Development ◽  
Cortical Microtubules ◽  
Organ Growth ◽  
Floral Organ Development ◽  
Floral Organs ◽  
Organ Identity

Floral organ development is fundamental to sexual reproduction in angiosperms. Many key floral regulators (most of which are transcription factors) have been identified and shown to modulate floral meristem determinacy and floral organ identity, but not much is known about the regulation of floral organ growth, which is a critical process by which organs to achieve appropriate morphologies and fulfill their functions. Spatial and temporal control of anisotropic cell expansion following initial cell proliferation is important for organ growth. Cortical microtubules are well known to have important roles in plant cell polar growth/expansion and have been reported to guide the growth and shape of sepals and petals. In this study, we identified two homolog proteins, QWRF1 and QWRF2, which are essential for floral organ growth and plant fertility. We found severely deformed morphologies and symmetries of various floral organs as well as a significant reduction in the seed setting rate in the qwrf1qwrf2 double mutant, although few flower development defects were seen in qwrf1 or qwrf2 single mutants. QWRF1 and QWRF2 display similar expression patterns and are both localized to microtubules in vitro and in vivo. Furthermore, we found altered cortical microtubule organization and arrangements in qwrf1qwrf2 cells, consistent with abnormal cell expansion in different floral organs, which eventually led to poor fertility. Our results suggest that QWRF1 and QWRF2 are likely microtubule-associated proteins with functional redundancy in fertility and floral organ development, which probably exert their effects via regulation of cortical microtubules and anisotropic cell expansion.

scholarly journals Integration of embryo-endosperm interaction into a holistic and dynamic picture of seed development using a rice mutant with notched-belly grains

2021 ◽  
Author(s):  
Yang Tao ◽  
Lu An ◽  
Feng Xiao ◽  
Ganghua Li ◽  
Yanfeng Ding ◽  
...  
Keyword(s):  
Seed Development ◽  
Staging System ◽  
Endosperm Development ◽  
Rice Grain ◽  
Developmental Transition ◽  
Rice Mutant ◽  
Gene Sets ◽  
Yield Formation ◽  
The Impact ◽  
Developmental Staging

ABSTRACTThe interaction between the embryo and endosperm affects seed development, an essential process in yield formation in crops such as rice. Signals that communicate between embryo and endosperm are largely unknown. Here we use the notched-belly (NB) mutant with impaired communication between embryo and endosperm to evaluate 1) the impact of embryo on developmental staging of the endosperm; 2) signaling pathways emanating from the embryo that regulate endosperm development. Hierachical clustering of mRNA datasets from embryo and endosperm samples collected through development in NB and wild type showed a delaying effect of the embryo on the developmental transition of the endosperm by extending the middle stage. K-means clustering further identified coexpression modules of gene sets specific for embryo and endosperm development. Combined gene expression and biochemical analysis showed that T6P-SnRK1, gibberellin and auxin signalling from the embryo regulate endosperm developmental transition. The data enable us to propose a new seed developmental staging system for rice and the most detailed signature of rice grain formation to date, that will direct genetic strategies for rice yield improvement.

Developmental Mechanisms of Fleshy Fruit Diversity in Rosaceae

2020 ◽  
Vol 71 (1) ◽  
pp. 547-573 ◽  
Author(s):  
Zhongchi Liu ◽  
Hong Ma ◽  
Sook Jung ◽  
Dorrie Main ◽  
Lei Guo
Keyword(s):  
Floral Organ ◽  
Fleshy Fruit ◽  
Pome Fruit ◽  
Floral Organs ◽  
Functional Studies ◽  
Genome Data ◽  
Organ Identity ◽  
Close Relatives ◽  
Evolutionary Paths ◽  
The Rose

Rosaceae (the rose family) is an economically important family that includes species prized for high-value fruits and ornamentals. The family also exhibits diverse fruit types, including drupe (peach), pome (apple), drupetum (raspberry), and achenetum (strawberry). Phylogenetic analysis and ancestral fruit-type reconstruction suggest independent evolutionary paths of multiple fleshy fruit types from dry fruits. A recent whole genome duplication in the Maleae/Pyreae tribe (with apple, pear, hawthorn, and close relatives; referred to as Maleae here) may have contributed to the evolution of pome fruit. MADS-box genes, known to regulate floral organ identity, are emerging as important regulators of fruit development. The differential competence of floral organs to respond to fertilization signals may explain the different abilities of floral organs to form fleshy fruit. Future comparative genomics and functional studies in closely related Rosaceae species with distinct fruit types will test hypotheses and provide insights into mechanisms of fleshy fruit diversity. These efforts will be facilitated by the wealth of genome data and resources in Rosaceae.

scholarly journals Green Synthesis of Zinc Oxide Nanoparticles: Fortification for Rice Grain Yield and Nutrients Uptake Enhancement

Molecules ◽  
2021 ◽  
Vol 26 (3) ◽  
pp. 584
Author(s):  
Omnia M. Elshayb ◽  
Khaled Y. Farroh ◽  
Heba E. Amin ◽  
Ayman M. Atta
Keyword(s):  
Zinc Oxide ◽  
Grain Yield ◽  
Field Experiments ◽  
Metal Oxide Nanoparticles ◽  
Foliar Spray ◽  
Yield Component ◽  
Oxide Nanoparticles ◽  
Rice Grain ◽  
Zno Nps ◽  
Agriculture Sector

Applications of metal oxide nanoparticles in the agriculture sector are being extensively included as the materials are considered superior. In the present work, zinc oxide nanoparticle (ZnO NPs), with a developing fertilizer, is applied in the fortification of rice grain yield and nutrient uptake enhancement. To evaluate the role of ZnO NP, two field experiments were conducted during the 2018 and 2019 seasons. ZnO NPs were small, nearly spherical, and their sizes equal to 31.4 nm, as proved via the dynamic light scattering technique. ZnO NPs were applied as a fertilizer in different concentrations, varying between 20 and 60 mg/L as a foliar spray. The mixture of ZnSO4 and ZnO NP40 ameliorated yield component and nutrients (N, K, and Zn) uptake was enhanced compared to traditional ZnSO4 treatment. Nevertheless, the uptake of the phosphorous element (P) was adversely affected by the treatment of ZnO NPs. Thus, treatment via utilizing ZnO NPs as a foliar with a very small amount (40 ppm) with of basal ZnSO4 led to a good improvement in agronomic and physiological features; eventually, higher yield and nutrient-enriched rice grain were obtained.

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