Evaluation of the Antioxidant and Wound-Healing Properties of Extracts from Different Parts of Hylocereus polyrhizus

Hylocereus polyrhizus cultivation started in Taiwan around the 1980s. The pulp of the fruit is edible and contains small, black, and soft seeds. The peel of the fruits are covered with bracts. The H. polyrhizus fruit is known to be rich in nutrients and minerals. To evaluate the potential applications of the agricultural wastes of H. polyrhizus, the stem, peel, and flower of H. polyrhizus were extracted with solutions of ethanol and water mixed in different ratios. Data was collected for the H. polyrhizus extract including the yield of total phenolics, the total flavonoids, and antioxidant activity, as determined by the 2-2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2′-azino-bis (3-ethylbenzothiazoline-6-sulphonic acid) (ABTS) radical scavenging assay. The protective effects of H. polyrhizus extract on DNA was investigated using an assay with the pUC19 plasmid. The cell proliferation and migration effects were evaluated in the NIH-3T3 fibroblast cell line. The greatest yield of extract from the stem of H. polyrhizus was 44.70 ± 1.77% which was obtained using 50% aqueous ethanol and the greatest yield of extract from the peel was 43.47% using distilled water. The stem extract, which was prepared with 95% aqueous ethanol, had the highest composition of phenolics and flavonoids as well as the best DPPH radical scavenging activity. The stem extract had excellent ABTS radical scavenging activity as well. The stem, peel, and flower extracts, which were prepared using 95% aqueous ethanol, showed excellent results in protecting themselves from DNA damage, similar to the effect of 0.3 mg/mL ferulic acid. None of the extracts were able to promote cell proliferation at concentrations of 250 μg/mL to 2,000 μg/mL in a 24 h period. The 1000 μg/mL stem and flower extracts in 95% aqueous ethanol promoted considerable cell migration after a 24 h period.

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Strobilanthes heyneanus root extract as a potential source for antioxidant and antimicrobial activity

Future Journal of Pharmaceutical Sciences ◽

10.1186/s43094-021-00242-2 ◽

2021 ◽

Vol 7 (1) ◽

Author(s):

Vijayakumar Sundaram ◽

Selvaraju Sadhasivam ◽

Sivaraj Chandrasekaran ◽

Raaman Nanjian ◽

Arjun Pandian

Keyword(s):

Dermal Fibroblast ◽

Radical Scavenging Activity ◽

Antioxidant Properties ◽

Radical Scavenging ◽

Reducing Power ◽

Fibroblast Cell ◽

Phytochemical Analysis ◽

Root Extract ◽

Scavenging Activity ◽

Medicinal Uses

Abstract Background Strobilanthes heyneanus (Nilgirianthus heyneanus) belongs to the family Acanthaceae that contains many species with potential for diverse medicinal uses. It is also called ‘Karun kurinji’ and is commonly found in the South-West regions of India. The species are commonly used in rheumatic complaints, sprain of the ankle, and hernia. The objectives of the study were to evaluate the antioxidant activity, phytochemical analysis, and antibacterial activities of the root extract of S. heyneanus. Results The radical scavenging and reduction assays such as DPPH● radical and OH● radical scavenging assays, as well as phosphomolybdenum reduction and Fe3+ reducing power assays, were determined for the root extract. The highest DPPH● radical scavenging activity was 88.23 ± 1.32 at 120 μg/mL concentration, and the calculated IC50 was 38.52 μg/mL concentration. The highest OH● radical scavenging activity was 51.28 ± 1.06 at 120 μg/mL concentration, and the calculated IC50 was 51.28 μg/mL concentration. The highest ABTS●+ radical scavenging activity was 91.28 ± 1.12 at 30 μg/mL concentration, and the calculated IC50 was 33.92 μg/mL concentration. The highest phosphomolybdenum reduction was 87.43 ± 0.90 at 120 μg/mL concentration, and the calculated RC50 was 24.74 μg/mL concentration. The highest Fe3+ reduction was 89.38 ± 0.98 at 120 μg/mL concentration, and the calculated RC50 was 31.06 μg/mL concentration. The antibacterial activity of S. heyneanus showed the highest zone of inhibition of 24 mm for Salmonella typhi with 500 μg/mL concentration. The cytotoxicity limits of the root extracts were tested by MTT assay using human dermal fibroblast cell lines, reflecting > 90% cell viability at a concentration of 500 μg/mL. Conclusions The current study showed that the root extract of S. heyneanus has better antioxidant properties and potential anti-bacterial compounds. The phytochemical analysis of the root extract showed the presence of alkaloids, steroids, terpenoids, phenols, flavonoids, tannins, saponins, glycosides, and carbohydrates, which are responsible for the antibacterial root extract of S. heyneanus synergistically.

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Free Radical Scavenging and Cytoprotective Activity of Salacia Euphlebia Merr

Natural Product Communications ◽

10.1177/1934578x0800300220 ◽

2008 ◽

Vol 3 (2) ◽

pp. 1934578X0800300

Author(s):

Sanan Subhadhirasakul ◽

Niwat Keawpradub ◽

Charuporn Promwong ◽

Supreeya Yuenyongsawad

Keyword(s):

Antioxidant Activity ◽

Free Radical ◽

Radical Scavenging Activity ◽

Radical Scavenging ◽

Free Radical Scavenging ◽

Scavenging Activity ◽

Medicine Use ◽

Cytoprotective Effect ◽

Cytoprotective Activity ◽

Stem Extract

The present study reports the antioxidant activity of Salacia euphlebia stem extract. To understand the antioxidant activity of this plant, four compounds were isolated and elucidated as vitexin, 15α-hydroxyfriedelan-3-one, siphulitol and mangiferin. Mangiferin showed high free radical scavenging activity (EC50 1.10 ± 0.18 μg/mL) in comparison with quercetin (EC50 1.35 ± 0.02 μg/mL), while vitexin (IC50 37.6 ± 1.1 μg/mL) showed a good cytoprotective effect compared with quercetin (IC50 76.1 ± 1.0 μg/mL). This is the first report of the activities and bioactive compounds of S. euphlebia. These results may scientifically explain the folk and alternative-medicine use of this plant in longevity formulas.

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Protective Effects of the Hydroethanolic Extract of Fridericia chica on Undifferentiated Human Neuroblastoma Cells Exposed to α-Zearalenol (α-ZEL) and β-Zearalenol (β-ZEL)

Toxins ◽

10.3390/toxins13110748 ◽

2021 ◽

Vol 13 (11) ◽

pp. 748

Author(s):

Neda Alvarez-Ortega ◽

Karina Caballero-Gallardo ◽

María Taboada-Alquerque ◽

Jackeline Franco ◽

Elena E. Stashenko ◽

...

Keyword(s):

Radical Scavenging Activity ◽

Antioxidant Properties ◽

Radical Scavenging ◽

Neuroblastoma Cells ◽

Scavenging Activity ◽

Protective Effects ◽

Human Neuroblastoma ◽

Dependent Relationship ◽

Dpph Method ◽

Hydroethanolic Extract

Fridericia chica (Bignoniaceae) is a traditional medicinal plant. The aim of this research was to determine the protective effects of the hydroethanolic extract from the F. chica leaves (HEFc) against the cytotoxicity of zearalenone (α-ZEL) and β-ZEL on SH-SY5Y cells. Free radical scavenging activity of HEFc was evaluated using the DPPH method. The cytotoxicity of both zearalenone metabolites and HEFc was examined using MTT test, as was the cytoprotective effects of the HEFc on cells treated with these mycotoxins. The chemical composition of HEFc was determined using UPLC-QTOF-MS/MS. HEFc elicited good DPPH radical scavenging activity following a concentration-dependent relationship. Cells exposed to α-ZEL exhibited a viability ˂50% after 48 h of treatment (25 and 50 µM), while those exposed to β-ZEL showed viability ˂50% (100 µM) and ˂25% (25-100 µM) after 24 and 48 h of exposure, respectively. HEFc showed a significant increase in cell viability after exposure to α-ZEL (25 and 50 µM) and β-ZEL (6–100 µM) (p < 0.05). UPLC-QTOF-MS/MS analyses allowed the identification of 10 phytochemical components in the HEFc. In short, the hydroethanolic extract of F. chica grown in Colombian Caribbean can protect against the effects of mycotoxins and it is a valuable source of compounds with antioxidant properties.

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EFFECT OF IRRADIATION ON TOTAL PHENOL AND CATECHINS CONTENTS AND RADICAL SCAVENGING ACTIVITY OF GREEN TEA LEAF AND STEM EXTRACT

Journal of Food Biochemistry ◽

10.1111/j.1745-4514.2008.00198.x ◽

2008 ◽

Vol 32 (6) ◽

pp. 782-794 ◽

Cited By ~ 5

Author(s):

S.C. LEE ◽

S.M. JEONG ◽

J.M. LEE ◽

A. JANG ◽

D.H. KIM ◽

...

Keyword(s):

Green Tea ◽

Radical Scavenging Activity ◽

Radical Scavenging ◽

Total Phenol ◽

Scavenging Activity ◽

Stem Extract ◽

Tea Leaf

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Antioxidant Potential and the Characterization of Arachis hypogaea Roots

BioMed Research International ◽

10.1155/2019/7073456 ◽

2019 ◽

Vol 2019 ◽

pp. 1-9 ◽

Cited By ~ 2

Author(s):

Samee Ullah ◽

Syed Ammar Hussain ◽

Faryal Shaukat ◽

Ahsan Hameed ◽

Wu Yang ◽

...

Keyword(s):

Arachis Hypogaea ◽

Aqueous Ethanol ◽

Antioxidant Activities ◽

Radical Scavenging Activity ◽

Radical Scavenging ◽

Dry Weight ◽

Absolute Methanol ◽

Total Phenolic ◽

Root Extract ◽

Scavenging Activity

Arachis hypogaea roots are used as traditional Chinese medicine to treat different ailments, and the present study involves the exploration and comparison of phenolic profile and antioxidant activities (ABTS+ and DPPH assay) of A. hypogaea root extract in different solvents. 70% aqueous acetone and 70% aqueous ethanol were proved to be the best solvents to recover total phenolic compounds, with a yield of 42.59 ± 1.96 and 41.34 ± 0.92 mg/g dry weight of extract, respectively. ABTS+ radical scavenging activity was the highest in 70% aqueous ethanol, while the absolute methanol extract showed the highest DPPH radical scavenging activity (29.50 ± 2.19 μg/mL). Furthermore, phytochemical profiling of 70% acetone extract of A. hypogaea roots was performed by LC-ESI-TOF-MS analysis which in turn indicated the presence of diverse compounds in the A. hypogaea root extract, namely, quinones, stilbenoids, and flavones and flavonoid glucosides.

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Antioxidant and Cytotoxic Properties of the Methanol Extract of Argyreia argentea Stem

Journal of Scientific Research ◽

10.3329/jsr.v2i3.4772 ◽

2010 ◽

Vol 2 (3) ◽

pp. 621 ◽

Cited By ~ 1

Author(s):

T. Ahsan ◽

M. A. Rahman ◽

N. U. Ahmed ◽

S. B. Uddin

Keyword(s):

Ascorbic Acid ◽

Methanol Extract ◽

Radical Scavenging Activity ◽

Radical Scavenging ◽

Free Radical Scavenging ◽

Scavenging Activity ◽

Lc50 Value ◽

Upper Limits ◽

Stem Extract ◽

Dose Dependent

The antioxidant potential of Argyreia argentea methanol stem extract was evaluated by 2,2-diphenyl-1-picrylhydrazyl free radical scavenging method. The extract showed very significant effect as antioxidant in comparison to ascorbic acid, a reference antioxidant. The radical scavenging activity showed by the extract was a dose dependent phenomenon. The extract at the concentration of 400 μg/ml among five different concentrations (10, 50, 100, 200, 400 μg/ml) showed the highest scavenging activity, 94.86%. The stem extract was also evaluated for its cytotoxic properties by measuring significant LC50 value in a Brine-Shrimp lethality bioassay. LC50 value of Argyreia argentea stem extract was found 939.18 μg/ml, at a 95% confidence limit, giving the lower and upper limits 608.98 and 2043.9 μg/ml, respectively. Keywords: Argyreia argentea; DPPH; Ascorbic acid; Scavenging activity; Gallic acid. © 2010 JSR Publications. ISSN: 2070-0237 (Print); 2070-0245 (Online). All rights reserved. DOI: 10.3329/jsr.v2i3.4772 J. Sci. Res. 2 (3), 523-530 (2010)

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Development of Thai Gac Fruit Extraction as a Multifunctional Cosmeceutical Ingredient for Antioxidant, Melanogenesis and Collagen Stimulating Activities

Key Engineering Materials ◽

10.4028/www.scientific.net/kem.819.104 ◽

2019 ◽

Vol 819 ◽

pp. 104-110

Author(s):

Sareeya Reungpatthanaphong ◽

Khanittha Chawananorasest ◽

Thanagon Kirdin ◽

Monchanok Bamrungchai ◽

Paiboon Reungpatthanaphong

Keyword(s):

Antioxidant Activity ◽

Cell Line ◽

Active Ingredient ◽

Human Fibroblast ◽

Radical Scavenging Activity ◽

Radical Scavenging ◽

Fibroblast Cell ◽

Scavenging Activity ◽

Antioxidant Power ◽

Human Fibroblast Cell

Gac fruit (Momordica cochinchinensis Spreng) is mostly found in southeast Asian countries. Previous studies have demonstrated that gac fruit is a major source of β-carotene, lycopene, and α-tocopherol. This study aimed to develop the extraction of Thai gac fruit as a potential active ingredient for antioxidant, melanogenesis, and collagen stimulating activities. In the present study, different parts of ripe Thai gac fruit, the peel, pulp, and seed membrane, were extracted using the accelerated solvent extraction (ASE) technique, an innovative plant extraction method. DPPH radical-scavenging activity, ABTS radical scavenging activity, and ferric reducing/antioxidant power (FRAP) assay were performed to compare the antioxidant activity derived from each part of the fruit. The effect of Thai gac fruit on melanogenesis and collagen stimulating activities was investigated using B16F1 and human fibroblast cell lines, respectively. The results demonstrate that the pulp (EC50 value of 7.42 ± 0.81 mg/mL) and the seed membrane (EC50 value of 76.43 ± 2.66 μg/mL) showed the strongest antioxidant activities in DPPH and ABTS assay, respectively. The peel clearly displayed the strongest antioxidant activity and significantly increased the FRAP value (247.67 ± 19.73 μM/mg). Furthermore, the peel showed the lowest melanin content (76.56 ± 7.13 % of control) in the B16F1 cell line, and clearly increased the most amount of collagen (21.87 ± 3.12%) in the human fibroblast cell line in comparison to the other fractions. Therefore, Thai gac fruit peel revealed the biological effects, including antioxidant, melanogenesis, and collagen stimulating activities. In conclusion, Thai gac fruit extracted using the ASE technique exhibited several effects that could be used as a potential active ingredient for cosmeceutical products, due to its antioxidant, melanogenesis, and collagen stimulating activities. Our findings provide potential uses of Thai gac fruit as a multifunctional cosmeceutical ingredient to be further explored in future studies.

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Collagen Peptides from Swim Bladders of Giant Croaker (Nibea japonica) and Their Protective Effects against H2O2-Induced Oxidative Damage toward Human Umbilical Vein Endothelial Cells

Marine Drugs ◽

10.3390/md18080430 ◽

2020 ◽

Vol 18 (8) ◽

pp. 430

Author(s):

Jiawen Zheng ◽

Xiaoxiao Tian ◽

Baogui Xu ◽

Falei Yuan ◽

Jianfang Gong ◽

...

Keyword(s):

Oxidative Damage ◽

Umbilical Vein ◽

Radical Scavenging Activity ◽

Radical Scavenging ◽

Extraction Process ◽

Enzyme Concentration ◽

Scavenging Activity ◽

Protective Effects ◽

Collagen Peptides ◽

Collagen Peptide

Five different proteases were used to hydrolyze the swim bladders of Nibea japonica and the hydrolysate treated by neutrase (collagen peptide named SNNHs) showed the highest DPPH radical scavenging activity. The extraction process of SNNHs was optimized by response surface methodology, and the optimal conditions were as follows: a temperature of 47.2 °C, a pH of 7.3 and an enzyme concentration of 1100 U/g, which resulted in the maximum DPPH clearance rate of 95.44%. Peptides with a Mw of less than 1 kDa (SNNH-1) were obtained by ultrafiltration, and exhibited good scavenging activity for hydroxyl radicals, ABTS radicals and superoxide anion radicals. Furthermore, SNNH-1 significantly promoted the proliferation of HUVECs, and the protective effect of SNNH-1 against oxidative damage of H2O2-induced HUVECs was investigated. The results indicated that all groups receiving SNNH-1 pretreatment showed an increase in GSH-Px, SOD, and CAT activities compared with the model group. In addition, SNNH-1 pretreatment reduced the levels of ROS and MDA in HUVECs with H2O2-induced oxidative damage. These results indicate that collagen peptides from swim bladders of Nibea japonica can significantly reduce the oxidative stress damage caused by H2O2 in HUVECs and provides a basis for the application of collagen peptides in the food industry, pharmaceuticals, and cosmetics.

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Physicochemical characteristics, viability of starters, total phenolics and antioxidant activities of functional yoghurt supplemented with extracts from Hylocereus polyrhizus, Hibiscus sabdariffa and Peristrophe bivalvis

Plant Science Today ◽

10.14719/pst.2021.8.1.1009 ◽

2021 ◽

Vol 8 (1) ◽

Author(s):

Minh Phuoc Nguyen Nguyen

Keyword(s):

Phenolic Content ◽

Radical Scavenging Activity ◽

Starter Culture ◽

Radical Scavenging ◽

Free Radical Scavenging ◽

Total Phenolic ◽

Hibiscus Sabdariffa ◽

Total Acidity ◽

Scavenging Activity ◽

Hylocereus Polyrhizus

Hylocereus polyrhizus, Hibiscus sabdariffa and Peristrophe bivalvis contain a great source of anthocyanin with excellent antioxidant property. Consumers highly appreciate the Yoghurt enriched with phenolics originated from plant. This research evaluated the possibility of yoghurt incorporated with pigment extract from Hylocereus polyrhizus, Hibiscus sabdariffa and Peristrophe bivalvis. Total acidity, syneresis, viability of starter culture, total phenolic content, free radical-scavenging activity of the enriched yoghurt were observed. Results showed that there was significant difference of total acidity, syneresis among yoghurt samples. Meanwhile there were a dramatic increasing of viability of starter culture, total phenolic content, free radical-scavenging activity in the enriched yoghurt compared to the control. Lactobacillus acidophilus retained high viability during storage due to functional prebiotic of plant extracts. Hibiscus sabdariffa extract would be a promising alternative for the enriched yoghurt with better health benefits.

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