scholarly journals Detection and Characterization of Viral Pathogens Associated with Reproductive Failure in Wild Boars in Central Italy

Animals ◽  
2021 ◽  
Vol 11 (2) ◽  
pp. 304
Author(s):  
Maria Irene Pacini ◽  
Mario Forzan ◽  
Giovanni Cilia ◽  
Fabrizio Bertelloni ◽  
Filippo Fratini ◽  
...  
Keyword(s):  
Wild Boar ◽  
Central Italy ◽  
Reproductive Failure ◽  
Porcine Circovirus ◽  
Phylogenetic Study ◽  
Swine Industry ◽  
Viral Pathogens ◽  
Wild Boars ◽  
In The Wild ◽  
Domestic Swine

Wild boar and domestic swine share several pathogens, including viruses responsible for reproductive failures, representing an important sanitary and economic risk for the swine industry. Among them, suid herpesvirus 1 (SuHV-1), porcine circovirus 2 (PCV2) and porcine parvovirus 1 (PPV1) are widely diffused in the wild boar population. Unfortunately, little is known about their pathogenetic mechanisms and impact on the reproductive parameters of wild animals. This study aims to investigate the presence of viruses responsible for reproductive failure in pregnant wild boar sows and their foetuses. The investigation was conducted on 46 pregnant wild boar and their foetuses by molecular analysis; a phylogenetic study was performed on the positive samples. All of the investigated pathogens were identified in sows, while only herpesvirus and circovirus were detected in the tissues of their foetuses. Phylogenetic analysis revealed that the viral sequences obtained from the positive wild boars were closely related to those previously identified in domestic swine belonging to the same study areas. The results suggest that SuHV-1 and PCV2 can infect wild boar foetuses, with a possible impact on wild boar reproductive performance. Moreover, our data highlight the importance of continuous monitoring of swine pathogens circulating in wild environments, so as to carry out adequate sanitary actions.

scholarly journals Prevalence and Antimicrobial Resistances of Salmonella spp. Isolated from Wild Boars in Liguria Region, Italy

Pathogens ◽  
2021 ◽  
Vol 10 (5) ◽  
pp. 568
Author(s):  
Elisabetta Razzuoli ◽  
Valeria Listorti ◽  
Isabella Martini ◽  
Laura Migone ◽  
Lucia Decastelli ◽  
...  
Keyword(s):  
Antimicrobial Resistance ◽  
Wild Boar ◽  
Antimicrobial Susceptibility ◽  
Potential Risk ◽  
Salmonella Enterica ◽  
Intestinal Tract ◽  
Wild Boar Population ◽  
Salmonella Spp ◽  
Wild Boars ◽  
In The Wild

Salmonella spp. is an important zoonotic agent. Wild boars might host this pathogen in the intestinal tract and might represent a risk for Salmonella spp. transmission to humans. Wild boars are widely spread in Liguria, due to the environmental characteristics of the region. The aim of the study was the isolation, typing, and investigation of antimicrobial susceptibility of the isolated strains of Salmonella spp. During the 2013–2017 hunting seasons, 4335 livers of wild boars were collected and analyzed for the presence of Salmonella spp. A total of 260 strains of Salmonella spp. were isolated and characterized, with a prevalence of 6%. The isolated strains belonged to all six Salmonella enterica subspecies. Most of them were identified as Salmonella enterica subs. enterica of which 31 different serotypes were identified. The dominating serotype identified was S. Enteritidis. The antimicrobial resistance profiles of the isolated strains were analyzed against sixteen molecules. Of the isolated strains, 94.6% were resistant to at least one of the tested antimicrobials. This study showed the circulation of resistant Salmonella spp. strains in the wild boar population living in this area of Italy, underling the potential risk for these animals to disseminate this pathogen and its antimicrobial resistances.

scholarly journals Trichinosis in Lublin Province in 2003-2010 on a Background of Wild Boar`S Population Dynamics

2012 ◽  
Vol 56 (1) ◽  
pp. 43-46
Author(s):  
Marian Flis
Keyword(s):  
Wild Boar ◽  
Fold Increase ◽  
Wild Boar Population ◽  
Wild Boars ◽  
Hospitalised Patients ◽  
Species Population ◽  
Wild Boar Meat ◽  
Population Interaction ◽  
Domestic Swine ◽  
Epidemiological Situation

Abstract The research aimed at evaluating the epizootic and epidemiological situation of trichinosis during the last 8 years in Lublin province on a background of progressing increase in wild boar population within the region and in the whole country. Data for the study were taken from the report on the results of the official examination of slaughter animals and meat, poultry, game, lagomorphs and aquaculture animals and six reports on the number of trichinosis cases found at wild boars and domestic swine. In order to evaluate the trichinosis epidemiological situation within the region, reports of the National Institute of Public Health-National Institute of Hygiene on the number of identified trichinosis cases in people, as well as the number of hospitalised patients were presented. In addition, information on the population and hunting achievement of wild boars in hunting circuits of Lublin province during the last 8 years was enclosed. The number of identified trichinosis cases in meat of wild boars from Lublin region increased 9 times, while the percentage of trichinosis occurrence in reference to the number of examined carcasses almost 3-fold. At the same period, the number of porcine carcasses, in which trichinosis was found, decreased by over 4 times. Over double increase in wild boar population on the studied area was observed during the evaluation. Dynamic increase in the population size - in an aspect of the species population interaction with the living habitat, and in the form of the increase in the number of damages of crops and cultivation fields - contributed to intensified hunting pressure towards the species expressed as almost 3-fold increase of wild boar hunting. Analysis of epizootic and epidemiological situation of Poland indicates that wild boar meat was the principal source of trichinosis during the studied period. Considering Lublin province, the number of identified trichinosis cases is still high as compared to eastern and central provinces. Meanwhile, when compared to western and northern Poland, the level of trichinosis invasion can be considered as low. Furthermore, the trichinosis morbidity among people, that does not exceed 0.18/100 thousand inhabitants, can be regarded as low. Nevertheless, the fact of underestimating the necessity of both wild boar’s and swine’s meat examination seems to be alarming

Detection and analysis of porcine circovirus type 1 in Hungarian wild boars: Short communication

2008 ◽  
Vol 56 (1) ◽  
pp. 139-144 ◽  
Author(s):  
Attila Cságola ◽  
István Kiss ◽  
Tamás Tuboly
Keyword(s):  
Porcine Circovirus Type ◽  
Porcine Circovirus ◽  
Wild Boars ◽  
Chain Reactions ◽  
Polymerase Chain Reactions ◽  
Pathogenic Virus ◽  
A Genome ◽  
Domestic Swine ◽  
Porcine Origin

Porcine circovirus type 1 (PCV1) is considered to be a non-pathogenic virus detected in cell cultures, vaccines or products used for cell culture preparations, all of them of porcine origin. Serological evidence and genetic studies suggested that PCV1 was widespread in domestic pigs. The presence of PCV1 in wild boars in Germany was also described using serological methods. This paper reports the first detection of PCV1 in Hungarian wild boars. Samples were collected at slaughterhouses and processed for polymerase chain reactions. The complete genome of PCV1 detected in the samples was determined and compared with the available PCV1 sequences of the GenBank database. The genomes formed two distinct clusters with minimum differences, where the Hungarian wild boar PCV1 (WB-H8) grouped together with genomes originating from domestic swine from China and Australia and with a genome detected in a porcine pepsin product.

scholarly journals Effect of time and temperature before chilling on the hygiene of carcasses in wild boar hunted in central Italy

2022 ◽  
Vol 10 (4) ◽  
Author(s):  
Roberto Barbani ◽  
Giulia Lalinga ◽  
Lia Bardasi ◽  
Raffaella Branciari ◽  
Dino Miraglia ◽  
...  
Keyword(s):  
Wild Boar ◽  
Environmental Temperature ◽  
Central Italy ◽  
Colony Count ◽  
Elapse Time ◽  
Moderate Correlation ◽  
Factors Affecting ◽  
Wild Boars ◽  
The Mean ◽  
Mean Time

The interest in certified game meat chains highlights the need for the evaluation and the management of factors affecting carcass hygiene along the peculiar steps of the production. The effects of time and temperature before chilling were specifically evaluated on aerobic colony count and Enterobacteriaceae count in hunted wild boar carcasses. Thirty wild boars were considered in two process steps where the hunted animal are still not chilled: after evisceration and just before chilling. Environmental temperature, carcass temperature and the elapse time between the two-step considered were registered. Furthermore, surface microbial loads were analyzed on the inner part of the carcasses. The mean time between the two sampling steps was 6 hours with an average environmental temperature of 20.49°C. A carcass temperature 9.6°C drop was observed during this period. In this lap of time aerobic colony count and Enterobacteriaceae count increased of 0.68 Log CFU/cm2 and 1.01 Log CFU/cm2 respectively, with a moderate correlation with the time but not with the temperature delta. The results reveal that the temperature conditions in central Italy hunting areas were not able to quickly reduce the carcass temperature and therefore the time between carcass evisceration and chilling should not exceed 6 hours.

scholarly journals West Nile Virus and Related Flavivirus in European Wild Boar (Sus scrofa), Latium Region, Italy: A Retrospective Study

Animals ◽  
2020 ◽  
Vol 10 (3) ◽  
pp. 494
Author(s):  
Angela Petruccelli ◽  
Tiziana Zottola ◽  
Gianmarco Ferrara ◽  
Valentina Iovane ◽  
Cristina Di Russo ◽  
...  
Keyword(s):  
West Nile Virus ◽  
Wild Boar ◽  
Specific Antibody ◽  
Central Italy ◽  
Enzyme Linked Immunosorbent Assay ◽  
Rt Pcr ◽  
Serum Samples ◽  
West Nile ◽  
Wild Boars ◽  
European Wild Boar

Background: A retrospective sero-survey for evidence of West Nile virus (WNV) infection in European wild boar (Sus scorfa) was conducted in the Latium region, Italy, on stored serum samples of the period November 2011 to January 2012. Methods: Sera were collected from 168 European wild boars and screened for antibodies to WNV and other Flaviviruses by competitive enzyme linked immunosorbent assay (cELISA). All sera positive for Flavivirus antibodies by cELISA were further examined by virus neutralization test (VNT). To test the presence of Flavivirus RNA in samples, an RT-PCR was performed using a pan-Flavivirus primers pair. Results: Thirteen wild boars (7.73%) were seropositive for Flaviviruses. The hemolysis of serum samples limited the interpretation of the VNT for 7 samples, confirming the presence of specific antibody against WNV in a single European wild boar serum sample. The presence of ELISA positive/VNT negative samples suggests the occurrence of non-neutralizing antibodies against WNV or other antigen-related Flaviviruses. No samples resulted positive for Flavivirus by RT-PCR assay. Conclusion: Although a moderately high percentage of animals with specific antibody for WNV has been detected in wild boar in other surveillance studies in Europe, this has not been reported previously in Italy. Together, these data indicate that European wild boar are exposed to WNV and/or other related-Flavivirus in central Italy and confirm the usefulness of wild ungulates, as suitable Flavivirus sentinels.

scholarly journals Free to Circulate: An Update on the Epidemiological Dynamics of Porcine Circovirus 2 (PCV-2) in Italy Reveals the Role of Local Spreading, Wild Populations, and Foreign Countries

Pathogens ◽  
2020 ◽  
Vol 9 (3) ◽  
pp. 221 ◽  
Author(s):  
Giovanni Franzo ◽  
Susanna Tinello ◽  
Laura Grassi ◽  
Claudia Maria Tucciarone ◽  
Matteo Legnardi ◽  
...  
Keyword(s):  
Haplotype Network ◽  
Porcine Circovirus ◽  
Wild Populations ◽  
Swine Industry ◽  
Wild Boars ◽  
Domestic Pigs ◽  
Detection Frequency ◽  
Foreign Countries ◽  
Porcine Circovirus 2 ◽  
Spread Dynamics

Porcine circovirus 2 (PCV-2) is one of the most impactful and widespread pathogens of the modern swine industry. Unlike other DNA viruses, PCV-2 is featured by a remarkable genetic variability, which has led to the emergence and recognition of different genotypes, some of which (PCV-2a, 2b, and 2d) have alternated over time. Currently, PCV-2d is considered the most prevalent genotype, and some evidence of differential virulence and vaccine efficacy have been reported. Despite the potential practical relevance, the data on PCV-2 epidemiology in Italy are quite outdated and do not quantify the actual circulation of this genotype in Italy. In the present study, 82 complete ORF2 sequences were obtained from domestic pigs and wild boars sampled in Northern Italy in the period 2013–2018 and merged with those previously obtained from Italy and other countries. A combination of phylogenetic, haplotype network, and phylodynamic analyses were used to genotype the collected strains and evaluate the temporal trend and the spatial and host spread dynamics. A rising number of PCV-2d detections was observed in domestic pigs, particularly since 2013, reaching a detection frequency comparable to PCV-2b. A similar picture was observed in wild boars, although a lower sequence number was available. Overall, the present study demonstrates the extreme complexity of PCV-2 molecular epidemiology in Italy, the significant spread across different regions, the recurrent introduction from foreign countries, and the frequent occurrence of recombination events. Although a higher viral flux occurred from domestic to wild populations than vice versa, wild boars seem to maintain PCV-2 infection and spread it over relatively long distances.

scholarly journals Epidemiological analysis of classical swine fever in wild boars in Japan

2021 ◽  
Vol 17 (1) ◽  
Author(s):  
Yumiko Shimizu ◽  
Yoko Hayama ◽  
Yoshinori Murato ◽  
Kotaro Sawai ◽  
Emi Yamaguchi ◽  
...  
Keyword(s):  
Wild Boar ◽  
Mixed Model ◽  
Linear Mixed Model ◽  
Classical Swine Fever ◽  
Disease Spread ◽  
Wild Boar Population ◽  
Wild Boars ◽  
First Case ◽  
Forest Continuity ◽  
In The Wild

Abstract Background Classical swine fever (CSF) is a contagious disease of pigs and wild boars that is transmitted through direct/indirect contact between animals or CSF virus-contaminated fomites. When the disease re-emerged in 2018 in Japan, a CSF-infected wild boar was reported shortly after the initial pig farm outbreak; subsequently, the disease spread widely. To control the disease spread among wild boars, intensive capturing, fencing, and oral bait vaccination were implemented with concomitant virological and serological surveillance. This study aimed to describe the disease spread in the wild boar population in Japan from September 2018, when the first case was reported, to March 2020, based on the surveillance data. We conducted statistical analyses using a generalized linear mixed model to identify factors associated with CSF infection among wild boars. Moreover, we descriptively assessed the effect of oral bait vaccination, which started in March 2019 in some municipalities in the affected areas. Results We observed a faster CSF infection spread in the wild boar population in Japan compared with the CSF epidemics in European countries. The infection probability was significantly higher in dead and adult animals. The influence of the multiple rounds of oral bait vaccination was not elucidated by the statistical modeling analyses. There was a decrease and increase in the proportion of infected and immune animals, respectively; however, the immunization in piglets remained insufficient after vaccination for 1 year. Conclusions Conditions regarding the wild boar habitat, including forest continuity, higher wild boar population density, and a larger proportion of susceptible piglets, were addressed to increase the infection risk in the wild boar population. These findings could improve the national control strategy against the CSF epidemic among wild boars.

scholarly journals African swine fever virus (ASFV) in Poland: Prevalence in a wild boar population (2017–2018)

2020 ◽  
Vol 65 (No. 4) ◽  
pp. 143-158 ◽  
Author(s):  
MP Frant ◽  
M Lyjak ◽  
L Bocian ◽  
A Barszcz ◽  
K Niemczuk ◽  
...  
Keyword(s):  
Wild Boar ◽  
Active Surveillance ◽  
African Swine Fever Virus ◽  
Winter Season ◽  
African Swine Fever ◽  
Disease Spread ◽  
Passive Surveillance ◽  
Wild Boar Population ◽  
Wild Boars ◽  
In The Wild

African swine fever (ASF) was first described in 1921 in Kenya. The latest epidemic of ASF started in 2007 in Georgia. The virus was introduced to Poland in 2014. Since the beginning of the epidemics, the National Veterinary Research Institute in Pulawy (NVRI) has been testing wild boar samples from restricted areas and other parts of Poland to conduct passive and active surveillance for ASFV in these groups of animals. The aim of this study was to summarise the last two years of the ASF epidemiological status in Poland and the attempt to find disease patterns in the wild boar population. The period between 2017 and 2018 brought a massive number of new ASF cases in Poland. The number of ASF-positive wild boars jumped from 91 in 2016 to 1 140 in 2017 (approximately a 12 × increase), and 2018 was even worse, with the disease affecting 4 083 animals (2 435 cases; one case could even be 10 animals or more if they are found in one place next to each other). The percentage of positive wild boars found dead (passive surveillance) in the restricted area increased in 2018 to 73.1% from 70.8% in 2017. The chance of obtaining positive results in this group was six times higher in December and 4.5 times higher in January than in August and September. The percentage of positive wild boars detected through active surveillance reached 1.5% in 2018. The data suggested that, not only in Poland, but also in other ASF-affected countries, during the epizootic stage of the disease spread the most important measure is an effective passive surveillance of dead wild boars especially, in the winter season rather than in the summer.

Life-history patterns in female wild boars (Sus scrofa): mother–daughter postweaning associations

2005 ◽  
Vol 83 (3) ◽  
pp. 474-480 ◽  
Author(s):  
G Kaminski ◽  
S Brandt ◽  
E Baubet ◽  
C Baudoin
Keyword(s):  
Social Organization ◽  
Wild Boar ◽  
Sus Scrofa ◽  
Overlapping Generations ◽  
Wild Population ◽  
Seasonal Fluctuations ◽  
Wild Boars ◽  
Before And After ◽  
In The Wild ◽  
Life History Patterns

Mother–daughter postweaning associations in wild boars (Sus scrofa L., 1758) were investigated using 12 years' data from a wild population in Champagne, France. In the wild boar, a polygynous ungulate species, females (i) can reproduce as soon as they are yearlings and (ii) generally have large litters, in contrast to many other ungulate species. It is generally thought that their social organization is centered around groups of adult females and their offspring, but genealogical relationships in female groups have never been studied. Hence this species is suitable for testing the hypothesis of a matrilineal social organization. We studied the occurrence and strength of mother–daughter associations before and after the first potential breeding of yearling females, using a total of 85 individuals. Seasonal fluctuations in associations were observed, but after weaning, daughters generally remained with the mother. When leaving their natal group, yearling females formed new kin groups with sisters. Two important factors involved in the postweaning associations were adult and yearling reproductive participation and maternal age. The present study constitutes the first clear demonstration that family groups, with overlapping generations of females, represent the typical social organization in a forest wild boar population.

scholarly journals Lack of Evidence on the Susceptibility of Ticks and Wild Rodent Species to PCV3 Infection

Pathogens ◽  
2020 ◽  
Vol 9 (9) ◽  
pp. 682
Author(s):  
Laura Grassi ◽  
Valentina Tagliapietra ◽  
Annapaola Rizzoli ◽  
Marco Martini ◽  
Michele Drigo ◽  
...  
Keyword(s):  
Roe Deer ◽  
Rodent Species ◽  
Porcine Circovirus ◽  
Myodes Glareolus ◽  
Wild Rodents ◽  
Wild Rodent ◽  
Serum Samples ◽  
Swine Industry ◽  
Wild Boars ◽  
Pcr Targeting

Porcine circovirus 3 (PCV3) is an emerging virus, first detected in 2016 and widespread in the swine industry. Although not considered a primary pathogen, PCV3 is potentially linked to several clinical conditions that threaten swine farming. Wild boars are considered the main reservoir species for PCV3 infection in the wild, but recent detection in roe deer, chamois and associated ticks has complicated our understanding of its epidemiology. Much emphasis has been placed on ticks, as competent vectors, and wild rodents, which typically feed immature tick stages. The aim of this study was to clarify whether wild rodent species and associated ticks are susceptible to PCV3 infection and involved in its spread. Wild small mammals’ serum samples and hosted ticks were, therefore, collected from areas where no wild boars were present and tested by PCR, targeting the PCV3 rep gene. A total of 90 yellow-necked mice (Apodemus flavicollis), two wood mice (A. sylvaticus), 26 bank voles (Myodes glareolus) and 262 Ixodes spp. ticks were investigated. PCV3 DNA was not detected in serum or in tick samples. These findings support the hypothesis that the investigated species do not have an actual role as PCV3 reservoirs. Further studies would be necessary to state whether these species, or others that we did not test, are involved in PCV3 infection spread—in particular when susceptible species share the same habitat.

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