Dietary Natural Plant Extracts Can Promote Growth and Modulate Oxidative Status of Senegalese Sole Postlarvae under Standard/Challenge Conditions

Plant extracts are known for their high content and diversity of polyphenols, which can improve fish oxidative status. A growth trial with Senegalese sole postlarvae (45 days after hatching) fed with one of four experimental diets—control (CTRL), and supplemented with curcumin (CC), green tea (GT), and grape seed (GS) extracts—was performed to assess if supplementation could improve growth performance and oxidative status. At the end of the growth trial, postlarvae were submitted to a thermal stress to assess their robustness. Sole growth was improved by CC and GS diets when compared to those fed the CTRL. CC and CTRL postlarvae presented the lowest oxidative damage (lipid peroxidation and protein carbonylation values). Stress-related biomarkers (heat shock protein 70 and glutathione-S-transferase) decreased in CC fish compared to those fed the CTRL diet, which might be due to a direct antioxidant capacity. In contrast, oxidative damage increased in GT and GS sole reared in standard conditions. However, after a thermal stress, GT and GS diets prevented the increase of protein carbonylation content and the decrease of antioxidant glutathione, depending on exposure time. Overall, dietary supplementation with natural extracts modulated oxidative status and stress response after a short/long-term exposure to temperature.

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Dietary Natural Plant Extracts Modulate Growth Performance and Antioxidant Status of Senegalese Sole Postlarvae

10.21203/rs.3.rs-117940/v2 ◽

2021 ◽

Author(s):

Maria J. Xavier ◽

Luís E.C. Conceição ◽

Luisa M.P. Valente ◽

Rita Colen ◽

Andreia C.M. Rodrigues ◽

...

Keyword(s):

Thermal Stress ◽

Oxidative Damage ◽

Antioxidant Capacity ◽

Green Tea ◽

Stress Resistance ◽

Grape Seed ◽

Oxidative Status ◽

Senegalese Sole ◽

Fish Diets ◽

Growth Trial

Abstract Background Oxidative stress has a direct impact on fish production, affecting both growth and health status. Plant based extracts, such as those from green tea, curcumin and grape seeds, are known for their abundant content and diversity of polyphenols. These bioactive compounds have a high antioxidant capacity making such extracts good additives to include in fish diets potentially improving the oxidative status of fish, and therefore enhancing growth and stress resistance of farmed fish.A growth trial with Senegalese sole postlarvae (45 days after hatching) fed with four experimental diets, a control (CTRL) and three supplemented with natural antioxidants, namely curcumin (CC), green tea (GT) and grape seed (GS) extracts, was performed during 25 days to check if these supplements could improve growth performance and fish oxidative status. Moreover, a thermal stress was applied at the end of the growth trial, to assess the effect of these supplemented diets in stress resistance of sole postlarvae.ResultsSole growth was improved by the dietary inclusion of CC and GS compared to CTRL. Postlarvae from CC and CTRL present the lowest values of oxidative damage (lipid peroxidation and protein carbonylation). Moreover, the fish fed CC showed a decrease in stress related biomarkers (heat shock protein 70 and glutathione-S-transferase) compare to CTRL, which might be due to direct antioxidant capacity. In contrast the supplementation of GT and GS increased the content of oxidative damage in sole reared in standard conditions. However, after a thermal stress exposure both GT and GS treatment seem to prevent the increase of protein carbonylation content and the decrease in the levels of antioxidant glutathione in sole, depending on the time of exposure. ConclusionsOverall, dietary supplementation with these natural extracts modulates oxidative status and stress response after a short/long term temperature increase. The incorporation of curcumin seems to be a safe additive to include in sole diets, enhancing growth and oxidative status. The supplementation of the studied doses of green tea and grape seed extracts in fish diets needs further research as they may act as pro-oxidant depending of the culture conditions.

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Dietary Natural Plant Extracts Modulate Growth Performance and Antioxidant Status of Senegalese Sole Postlarvae

10.21203/rs.3.rs-117940/v1 ◽

2020 ◽

Author(s):

Maria Xavier ◽

Luís Conceição ◽

Luisa Valente ◽

Rita Colen ◽

Andreia Rodrigues ◽

...

Keyword(s):

Thermal Stress ◽

Oxidative Damage ◽

Antioxidant Capacity ◽

Green Tea ◽

Stress Resistance ◽

Grape Seed ◽

Oxidative Status ◽

Senegalese Sole ◽

Fish Diets ◽

Growth Trial

Abstract Oxidative stress has a direct impact on fish production, affecting both growth and health status. Plant based extracts, such as those from green tea, curcumin and grape seeds, are known for their abundant content and diversity of polyphenols. These bioactive compounds have a high antioxidant capacity making such extracts good additives to include in fish diets potentially improving the oxidative status of fish, and therefore enhancing growth and stress resistance of farmed fish. A growth trial with Senegalese sole postlarvae (45 DAH) fed with four experimental diets, a control (CTRL) and three supplemented with natural antioxidants, namely curcumin (CC), green tea (GT) and grape seed (GS) extracts, was performed during 25 days to check if these supplements could improve growth performance and fish oxidative status. Moreover, a thermal stress was applied at the end of the growth trial, to assess the effect of these supplemented diets in stress resistance of sole juveniles.Sole growth was improved by the dietary inclusion of CC and GS compared to CTRL. Juveniles from CC and CTRL present the lowest values of oxidative damage (lipid peroxidation (LPO) and protein carbonylation (PC)). Moreover, the fish fed CC showed a decrease in stress related biomarkers (heat shock protein 70 (HSP70) and glutathione-S-transferase (GST)) compare to CTRL, which might be due to direct antioxidant capacity. In contrast the supplementation of GT and GS increased the content of oxidative damage in sole reared in standard conditions. However, after a thermal stress exposure both GT and GS treatment seem to prevent the increase of PC and decrease of antioxidant glutathione (GSH) in sole and depending on the time of exposure. In conclusion, dietary supplementation with these natural extracts modulates oxidative status and stress response after a short/long term temperature increase. The incorporation of curcumin seems to be a safe additive to include in sole diets, enhancing growth and oxidative status. The supplementation of the studied doses of green tea and grape seed extracts in fish diets needs further research as they may act as pro-oxidant depending of the culture conditions.

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Melatonin protects against cadmium-induced oxidative damage in different tissues of rat: a mechanistic insight

Melatonin Research ◽

10.32794/mr11250018 ◽

2019 ◽

Vol 2 (2) ◽

pp. 1-21 ◽

Cited By ~ 3

Author(s):

Elina Mitra ◽

Bharati Bhattacharjee ◽

Palash Kumar Pal ◽

Arnab Kumar Ghosh ◽

Sanatan Mishra ◽

...

Keyword(s):

Cytochrome C ◽

Oxidative Damage ◽

Protein Carbonyl ◽

Environmental Pollutant ◽

Glutathione S Transferase ◽

Protein Carbonyl Content ◽

Wide Range ◽

Liver And Kidney ◽

Nadh Cytochrome ◽

Cd Exposure

Cadmium (Cd) is a notorious environmental pollutant known for its wide range of toxicities to organisms. Thus, the present study is designed to examine whether melatonin, a potent antioxidant, protects against Cd-induced oxidative damage in the heart, liver and kidney of rats. Cd treatment at a dose of 0.44 mg/kg for 15 days caused severe damage in all these organs. These included significantly increased activities of SGPT, SGOT, lactate dehydrogenase- 1 and 5 and ALP and levels of total lactate, creatinine, lipid peroxidation, protein carbonyl content and reduced glutathione while the activities of superoxide dismutases, catalase, glutathione peroxidase, glutathione reductase and glutathione-S-transferase along with mitochondrial pyruvate dehydrogenase, isocitrate dehydrogenase, α-keto glutarate dehydrogenase, succinate dehydrogenase, NADH-cytochrome-c-oxidoreductase and cytochrome-c-oxidase were significantly reduced by Cd. However, if melatonin was given orally 30 min before Cd injection, all these alterations induced by Cd were significantly preserved by melatonin. Histological observations also demonstrated that Cd exposure caused cellular lesions, promoting necrotic or apoptotic changes. Notably, all these changes were significantly protected by melatonin. The results suggest that melatonin is a beneficial molecule to ameliorate Cd-induced oxidative damage in the heart, liver and kidney tissues of rats with its powerful antioxidant capacity, heavy metal chelating activity and competition of binding sites with Cd to the GSH and catalase.

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Chemoprotective Effects of Propolis on Aflatoxin B1-Induced Hepatotoxicity in Rats: Oxidative Damage and Hepatotoxicity by Modulating TP53, Oxidative Stress

Current Proteomics ◽

10.2174/1570164617666190925121720 ◽

2020 ◽

Vol 17 (3) ◽

pp. 191-199

Author(s):

Seval Yilmaz ◽

Fatih Mehmet Kandemir ◽

Emre Kaya ◽

Mustafa Ozkaraca

Keyword(s):

Oxidative Stress ◽

Gene Expression ◽

Oxidative Damage ◽

Aflatoxin B1 ◽

Tp53 Gene ◽

Control Group ◽

Glutathione S Transferase ◽

Gene Expressions ◽

Cat Gene ◽

Gst Activity

Objective: This study aimed to detect hepatic oxidative damage caused by aflatoxin B1 (AFB1), as well as to examine how propolis protects against hepatotoxic effects of AFB1. Method: Rats were split into four groups as control group, AFB1 group, propolis group, AFB1+ propolis group. Results: There was significant increase in malondialdehyde (MDA) level and tumor suppressor protein (TP53) gene expression, Glutathione (GSH) level, Catalase (CAT) activity, CAT gene expression decreased in AFB1 group in blood. MDA level and Glutathione-S-Transferase (GST) activity, GST and TP53 gene expressions increased in AFB1 group, whereas GSH level and CAT activity alongside CAT gene expression decreased in liver. AFB1+propolis group showed significant decrease in MDA level, GST activity, TP53 and GST gene expressions, GSH level and CAT activity and CAT gene expression increased in liver compared to AFB1 group. Conclusion: These results suggest that propolis may potentially be natural agent that prevents AFB1- induced oxidative stress and hepatotoxicity.

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Natural Antioxidants in Anemia Treatment

International Journal of Molecular Sciences ◽

10.3390/ijms22041883 ◽

2021 ◽

Vol 22 (4) ◽

pp. 1883

Author(s):

Coralia Cotoraci ◽

Alina Ciceu ◽

Alciona Sasu ◽

Anca Hermenean

Keyword(s):

Plant Extracts ◽

Iron Homeostasis ◽

Therapeutic Potential ◽

Public Health Problem ◽

Natural Antioxidants ◽

Peptide Hormone ◽

Biologically Active ◽

Major Public Health Problem ◽

Stomach Pain

Anemia, characterized by a decrease of the hemoglobin level in the blood and a reduction in carrying capacity of oxygen, is a major public health problem which affects people of all ages. The methods used to treat anemia are blood transfusion and oral administration of iron-based supplements, but these treatments are associated with a number of side effects, such as nausea, vomiting, constipation, and stomach pain, which limit its long-term use. In addition, oral iron supplements are poorly absorbed in the intestinal tract, due to overexpression of hepcidin, a peptide hormone that plays a central role in iron homeostasis. In this review, we conducted an analysis of the literature on biologically active compounds and plant extracts used in the treatment of various types of anemia. The purpose of this review is to provide up-to-date information on the use of these compounds and plant extracts, in order to explore their therapeutic potential. The advantage of using them is that they are available from natural resources and can be used as main, alternative, or adjuvant therapies in many diseases, such as various types of anemia.

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Effects of Desiccation on Metamorphic Climax in Bombina variegata: Changes in Levels and Patterns of Oxidative Stress Parameters

Animals ◽

10.3390/ani11040953 ◽

2021 ◽

Vol 11 (4) ◽

pp. 953

Author(s):

Tamara G. Petrović ◽

Ana Kijanović ◽

Nataša Kolarov Kolarov Tomašević ◽

Jelena P. Gavrić ◽

Svetlana G. Despotović ◽

...

Keyword(s):

Oxidative Stress ◽

Oxidative Damage ◽

Antioxidant System ◽

Water Availability ◽

System Control ◽

Glutathione S Transferase ◽

Oxidative Stress Parameters ◽

Metamorphic Climax ◽

Bombina Variegata ◽

Stress Parameters

In this paper, we examined how the oxidative status (antioxidant system and oxidative damage) of Bombina variegata larvae changed during the metamorphic climax (Gosner stages: 42—beginning, 44—middle and 46—end) and compared the patterns and levels of oxidative stress parameters between individuals developing under constant water availability (control) and those developing under decreasing water availability (desiccation group). Our results revealed that larvae developing under decreasing water availability exhibited increased oxidative damage in the middle and end stages. This was followed by lower levels of glutathione in stages 44 and 46, as well as lower values of catalase, glutathione peroxidase, glutathione S-transferase and sulfhydryl groups in stage 46 (all in relation to control animals). Comparison between stages 42, 44 and 46 within treatments showed that individuals in the last stage demonstrated the highest intensities of lipid oxidative damage in both the control and desiccation groups. As for the parameters of the antioxidant system, control individuals displayed greater variety in response to changes induced by metamorphic climax than individuals exposed to desiccation treatment. The overall decrease in water availability during development led to increased oxidative stress and modifications in the pattern of AOS response to changes induced by metamorphic climax in larvae of B. variegata.

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