scholarly journals Determination of Optimal Doses and Minimum Effective Concentrations of Tricaine Methanesulfonate, 2-Phenoxyethanol and Eugenol for Laboratory Managements in Nile Tilapia (Oreochromis niloticus)

Animals ◽  
2021 ◽  
Vol 11 (6) ◽  
pp. 1521
Author(s):  
Tirawat Rairat ◽  
Yu Chi ◽  
Chia-Yu Hsieh ◽  
Yi-Kai Liu ◽  
Niti Chuchird ◽  
...  
Keyword(s):  
Nile Tilapia ◽  
Optimal Dose ◽  
Stage Iii ◽  
Serum Concentrations ◽  
Minimum Effective Concentration ◽  
Anesthetic Agents ◽  
Nile Tilapia Oreochromis Niloticus ◽  
Recovery Times ◽  
Tricaine Methanesulfonate

Anesthetic agents are often used in fish experiments to reduce the stress and struggle and to improve animal welfare. The present study aimed to determine the optimal doses and serum minimum effective concentration (MEC) of tricaine methanesulfonate (MS-222), 2-phenoxyethanol (2-PE), and eugenol (EUG) in Nile tilapia. Twenty-one fish were immersed in three different doses of each anesthetic and the minimal dose that produce stage III anesthesia within 5 min, maintain anesthesia status for 3 min, and recover within 5 min was considered the optimal dose. The serum concentrations of anesthetics immediately after the fish reached stage III anesthesia was defined as the MEC. The results revealed that the anesthetics dose-dependently shorten the induction time while the effect of doses on the recovery times were variable. The determined optimal doses for MS-222, 2-PE, and EUG were 300, 900, and 90 ppm, respectively. The MECs were 70, 263, and 53 µg/mL, respectively, about two to four times lower than the optimal doses and were independent of the doses. After immersion stopped, the serum concentrations decreased by >90% within the first hour and >99% after 4 h. Our research provides useful information for a smooth fish handling and design for researches requiring stage III anesthesia.

scholarly journals Determination of presence and quantification of cadmium, lead and copper in Nile tilapia (Oreochromis niloticus) fillets obtained from three cold storage plants in the state of Parana, Brazil

2011 ◽  
Vol 31 (2) ◽  
pp. 361-365 ◽  
Author(s):  
Alexandre Nobuhiro Tajiri ◽  
Daisy Pontes Netto ◽  
Márcia Sassahara ◽  
Mari Sylmara Martins Rodrigues ◽  
Cristina Arduini Cavalcanti de Arruda
Keyword(s):  
Cold Storage ◽  
Oreochromis Niloticus ◽  
Nile Tilapia ◽  
The State ◽  
Nile Tilapia Oreochromis Niloticus ◽  
Cadmium Lead

Characterisation of thermostable trypsin and determination of trypsin isozymes from intestine of Nile tilapia (Oreochromis niloticus L.)

2012 ◽  
Vol 134 (3) ◽  
pp. 1533-1541 ◽  
Author(s):  
Sasimanas Unajak ◽  
Piyachat Meesawat ◽  
Atchara Paemanee ◽  
Nontawith Areechon ◽  
Arunee Engkagul ◽  
...  
Keyword(s):  
Oreochromis Niloticus ◽  
Nile Tilapia ◽  
Nile Tilapia Oreochromis Niloticus ◽  
Trypsin Isozymes

scholarly journals KANDUNGAN LOGAM BERAT TIMBAL (Pb) PADA IKAN NILA (Oreochromis niloticus) DI SUNGAI TENGGANG, SEMARANG, JAWA TENGAH Heavy Metal Concentration of Pb in Nile tilapia (Oreochromis niloticus) in the Tenggang River, Semarang, Central Java

2019 ◽  
Vol 8 (3) ◽  
pp. 242-249
Author(s):  
Dana Yuli Agustina ◽  
Djoko Suprapto ◽  
Sigit Febrianto
Keyword(s):  
Heavy Metal ◽  
Oreochromis Niloticus ◽  
Nile Tilapia ◽  
Bioconcentration Factor ◽  
Heavy Metal Concentration ◽  
Purposive Sampling ◽  
Central Java ◽  
Nile Tilapia Oreochromis Niloticus ◽  
Paint Industry

ABSTRAK Timbal merupakan logam berat yang bersifat toksik dan biasanya bersumber dari industri cat, baterai dan percetakan. Sungai Tenggang berdekatan dengan Lingkungan Industri Kecil (LIK) dan pemukiman sehingga meningkatkan limbah ke dalam perairan termasuk logam berat. Hal tersebut menjadi alasan mengapa penelitian mengenai kandungan logam berat timbal pada ikan nila (Oreochromis niloticus) di Sungai tenggang, Semarang, Jawa Tengah ini dilaksanakan. Penelitian ini bertujuan untuk mengetahui kandungan logam berat timbal pada air dan ikan nila, mengetahui nilai biokonsentrasi faktor ikan nila serta mengetahui batas konsumsi mingguan daging ikan nila. Penelitian ini dilaksanakan pada bulan April 2019. Metode yang digunakan adalah metode deskriptif dengan pendekatan secara kuantitatif serta sampling menggunakan metode purposive sampling. Hasil perhitungan konsentrasi logam berat Pb dalam air rata-rata sebesar 0,155 mg/l. Konsentrasi logam berat Pb dalam daging ikan nila rata-rata 2,35 mg/kg. Hasil perhitungan bioconcentration factor (BCF) berkisar antara 12,03 – 17,17.  Hasil perhitungan berat maksimal konsumsi ikan nila mingguan diperoleh rata-rata sebesar 0,63 kg/minggu. Berdasarkan hasil yang telah diperoleh dapat disimpulkan bahwa kadar konsentrasi logam berat timbal pada air dan ikan nila di Sungai Tenggang tergolong cukup tinggi serta sudah melebihi ambang batas baku mutu yang telah ditentukan. Angka BCF logam berat timbal pada ikan nila termasuk dalam kategori tingkat akumulatif rendah (BCF < 100). ABSTRACT Plumbum is heavy metal that is toxic and source from paint industry, battery and printing. Tenggang river near with small industrial environment (LIK) and settlement so that increasing the waste entering to river including heavy metal waste. This is the reason for this research do. This study aims to determine the heavy metal Pb of water and Nile tilapia meat, bioconcentration factor of nile and knowing the limit of weekly consumption of  nile tilapia meat (MTI). The study was conducted in April 2019. The method used was a descriptive method with the determination of sampling using purposive sampling. The result of heavy metal Pb concentration in water averaged by 0,155 mg/l. Concentration of heavy metal Pb in Nile tilapia (Oreochromis niloticus) meat averaged by 2,35 mg/kg. The calculation result of bioconcentration factor (BCF) is range between 12,03 – 17,17. The maximum limit result of weekly fish consumption is averaged by 0,63 kg/minggu. Based on the results obtained it can be concluded that concentration of heavy metal Pb in water and nile tilapia tenggang river quite high and has reached above the permisible limit. BCF number of heavy metal in nile tilapia fall into the category of low accumulative levels (BCF < 100).

scholarly journals Influence of glyceryl guaiacolate ether on anesthetics in tilapia compared to benzocaine and eugenol

2014 ◽  
pp. 3944-3953 ◽  
Author(s):  
Geovana R. Cosenza ◽  
Gustavo S. Claudiano ◽  
Paulo F. Marcusso ◽  
Silas F. Eto ◽  
Wilson G. Manrique ◽  
...  
Keyword(s):  
Total Protein ◽  
Nile Tilapia ◽  
Hematological Parameters ◽  
Running Water ◽  
Rapid Induction ◽  
Fish Blood ◽  
Nile Tilapia Oreochromis Niloticus ◽  
Recovery Times ◽  
The Times ◽  
Hematological Changes

ABSTRACTObjective. The study aimed to investigate the effectiveness of glyceryl guaiacolate ether (GGE) and compare the times of induction, recovery, hematological changes, total protein and glycaemia among anesthetics in Nile tilapia, Oreochromis niloticus. Materials and methods. A total of 60 tilapia distributed in 3 aquariums (N=20) were used, which formed the group benzocaine (100 mg/L), eugenol (50 mg/L) and guaiacol glyceryl ether (9.000 mg/L). After the induction of anesthesia fish blood samples were collected to determine the complete hemogram and glycemia. Then the animals were placed in aquariums with running water for assessing the anesthesia recovery. Results. It was verified that GGE showed longer induction and recovery times as well a significant increase (p<0.05) of glycemia, when compared with the other groups (p<0.05). The concentration of total protein did not differ between groups (p>0.05). An increase in the number of monocytes in the group treated with benzocaine (p <0.05) was observed in the analysis of the hematological parameters with no difference between groups for other variables. Conclusions. Eugenol and benzocaine allow rapid induction and recovery in Nile tilapia, without evidence of stress during handling and GGE showed high induction and recovery times, being inadequate for anesthetic use in Nile tilapia.

scholarly journals UKURAN PERTAMA KALI MATANG GONAD DAN SELEKTIVITAS JARING INSANG IKAN NILA (Oreochromis niloticus) DI WADUK JATILUHUR, JAWA BARAT

2019 ◽  
Vol 18 (3) ◽  
Author(s):  
Andri Warsa ◽  
Lismining Pujiyani Astuti
Keyword(s):  
Fisheries Management ◽  
Oreochromis Niloticus ◽  
Nile Tilapia ◽  
Mesh Size ◽  
Fishing Gear ◽  
Fish Size ◽  
Total Length ◽  
Nile Tilapia Oreochromis Niloticus ◽  
Size Interval

A gillnet is a common fishing gear for exploitation and fish capture at Jatiluhur Reservoir. The fish size captured by the gillnet depends on the mesh size for its selective fishing gear. Determination of gillnet mesh size is one of effort for fisheries management.  The management of nile tilapia (Oreochromis niloticus) is based on gillnet selectivity and reproductive biology of nile tilapia. The aim of the research was to estimate length at the first mature (Lm) and gillnet selectivity for nile tilapia. The research was conducted at February-September 2017 through experimental fishing using gillnet with 1.0–4.0 mesh size (interval 0.5 inch). The gillnet installation was performed at six stations, namely Cihuni-Cibadak, Sungai Cikanyayan, Sungai Ciririp, Sungai Cihonje, Pasir Kole and Cilalawi. The Lm of nile tilapia was 18.3 and 23.3 cm for female and male, respectively. The total length retained in the gillnet with the highest probability for mesh size 1.0; 1.5; 2.0; 2.5; 3.0; 3.5 and 4.0 inches were 9.5; 12.5; 14.5; 18.5; 20.5; 26.5 and 30.5 cm, respectively. The minimum mesh size used for nile tilapia exploitation was ≥3 inches because it was able to catch nile tilapia with the total length larger than Lm (Lc>Lm) and with an SPR value of > 40% so that the tilapia resource in the Jatiluhur Reservoir remains sustainable.  

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