scholarly journals Salivary Chromogranin A (CgA) Response to the Noradrenaline Transporter Blocker Atomoxetine in Dogs

Animals ◽  
2021 ◽  
Vol 11 (10) ◽  
pp. 2844
Author(s):  
Takanori Kooriyama ◽  
Abhijit Mukhopadhyay ◽  
George E. Moore ◽  
Niwako Ogata
Keyword(s):  
Chromogranin A ◽  
Saliva Sample ◽  
Potential Candidate ◽  
Sample Collection ◽  
Treatment Groups ◽  
Noradrenaline Transporter ◽  
Stress Markers ◽  
Behavior Observation ◽  
Treatment Administration ◽  
And Behavior

Since salivary chromogranin A (CgA) is one of the known sympathetic adrenomedullar system (SAM) stress markers in humans and pigs, this study aimed to investigate whether salivary CgA in dogs reflects SAM activation. Our hypothesis was that salivary CgA would increase when central noradrenaline was pharmacologically induced. A selective noradrenaline transporter blocker, atomoxetine, was orally administered without causing any aversive responses in nine laboratory dogs to see if it would increase salivary CgA. Three treatment groups (i.e., atomoxetine, placebo, and pre-administration of a selective alpha-2 adrenoreceptor agonist (dexmedetomidine) followed by atomoxetine) were prepared with a randomized crossover design. Saliva sample collection, heart rate measurement and behavior observation were performed at Time 0 (baseline) and at 30, 60, 90 and 150 min after each treatment administration. The results demonstrated that salivary CgA significantly increased at 90 and 150 min in the atomoxetine treatment (p < 0.05), whereas it was not observed in the other two treatments. The present study showed that salivary CgA was increased by atomoxetine-induced SAM activation. However, this increase was blocked if dexmedetomidine was pre-administered. Overall, the results indicate that salivary CgA is a potential candidate for SAM-mediated stress markers in dogs. Further study to determine the dynamics of salivary CgA will be helpful in its practical use.

scholarly journals Lawsonia intracellularis infected enterocytes lack sucrase-isomaltase which contributes to reduced pig digestive capacity

2021 ◽  
Vol 52 (1) ◽  
Author(s):  
Emma T. Helm ◽  
Eric R. Burrough ◽  
Fernando L. Leite ◽  
Nicholas K. Gabler
Keyword(s):  
Dry Matter ◽  
Post Inoculation ◽  
Intestinal Function ◽  
Lawsonia Intracellularis ◽  
Sample Collection ◽  
Treatment Groups ◽  
Swine Herds ◽  
The Impact ◽  
Lesion Severity

AbstractLawsonia intracellularis is endemic to swine herds worldwide, however much is still unknown regarding its impact on intestinal function. Thus, this study aimed to characterize the impact of L. intracellularis on digestive function, and how vaccination mitigates these impacts. Thirty-six L. intracellularis negative barrows were assigned to treatment groups (n  =  12/trt): (1) nonvaccinated, L. intracellularis negative (NC); (2) nonvaccinated, L intracellularis challenged (PC); and (3) L. intracellularis challenged, vaccinated (Enterisol® Ileitis, Boehringer Ingelheim) 7 weeks pre-challenge (VAC). On days post-inoculation (dpi) 0 PC and VAC pigs were inoculated with L. intracellularis. From dpi 19–21 fecal samples were collected for apparent total tract digestibility (ATTD) and at dpi 21, pigs were euthanized for sample collection. Post-inoculation, ADG was reduced in PC pigs compared with NC (41%, P  <  0.001) and VAC (25%, P  <  0.001) pigs. Ileal gross lesion severity was greater in PC pigs compared with NC (P  =  0.003) and VAC (P  =  0.018) pigs. Dry matter, organic matter, nitrogen, and energy ATTD were reduced in PC pigs compared with NC pigs (P  ≤  0.001 for all). RNAscope in situ hybridization revealed abolition of sucrase-isomaltase transcript in the ileum of PC pigs compared with NC and VAC pigs (P  <  0.01). Conversely, abundance of stem cell signaling markers Wnt3, Hes1, and p27Kip1 were increased in PC pigs compared with NC pigs (P  ≤  0.085). Taken together, these data demonstrate that reduced digestibility during L. intracellularis challenge is partially driven by abolition of digestive machinery in lesioned tissue. Further, vaccination mitigated several of these effects, likely from lower bacterial burden and reduced disease severity.

Relationship Between Lifestyle Scores and Salivary Stress Markers Cortisol and Chromogranin A

2005 ◽  
Vol 60 (5) ◽  
pp. 266-269 ◽  
Author(s):  
Masahiro Toda ◽  
Rei Den ◽  
Shingo Nagasawa ◽  
Kazuyuki Kitamura ◽  
Kanehisa Morimoto
Keyword(s):  
Chromogranin A ◽  
Stress Markers

LEARNING CLEAN AND HEALTHY LIVING BEHAVIOR WITH POP-UP BOOK AND POSTER MEDIA ON CHILDREN AGES 6-12 YEARS AT ELEMENTARY SCHOOL, MALANG

2021 ◽  
Vol 5 (2) ◽  
pp. 174
Author(s):  
Naimah Naimah ◽  
Wahyu Setyaningsih
Keyword(s):  
Statistical Testing ◽  
Personal Hygiene ◽  
Healthy Living ◽  
Cross Sectional ◽  
Treatment Groups ◽  
Health Quality ◽  
And Behavior ◽  
Manova Analysis ◽  
Attitudes And Behavior ◽  
Improve Health

  Abstract Clean and Healthy Living Behavior (CHLB) is an effort to improve health quality through clean and healthy daily life behaviors. The problem of health behavior in children is related to personal hygiene, the environment, and the emergence of various diseases that often attack children. One of the preventive measures is to teach CHLB to children as early as possible by using interesting and easy-to-understand media for children. This study aims to determine the effect of a combination of a pop-up book and poster media on the ability of CHLB children aged 6-12 years. This study used a cross-sectional approach with 90 respondents divided into 3 groups. Collecting data using a questionnaire and analyzed by univariate, bivariate, and multivariate. The results showed that 66.67% of respondents had knowledge in the sufficient category and 10% in the less category before being given a combination of the two methods. Each respondent experienced a significant change in their knowledge. Statistical testing with MANOVA analysis on the comparison between treatment groups on knowledge, attitudes, and behavior obtained a calculated F value of 172,000 with a significance of <0.001 indicating that there were significant differences between treatment groups on knowledge, attitudes, and behavior. The use of pop-up books and posters can be used in the learning process because they have a major impact on increasing children's knowledge, attitudes, and behavior in an effort to maintain health through clean and healthy living behaviors. 

Impact of Transportation of Feedlot Cattle to the Harvest Facility on the Prevalence of Escherichia coli O157:H7, Salmonella, and Total Aerobic Microorganisms on Hides

2007 ◽  
Vol 70 (1) ◽  
pp. 17-21 ◽  
Author(s):  
A. L. REICKS ◽  
M. M. BRASHEARS ◽  
K. D. ADAMS ◽  
J. C. BROOKS ◽  
J. R. BLANTON ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Feedlot Cattle ◽  
Lower Percentage ◽  
Escherichia Coli O157 ◽  
Sample Collection ◽  
Treatment Groups ◽  
E Coli ◽  
Sampling Locations ◽  
Before And After

Prevalences of Escherichia coli O157:H7, Salmonella, and total aerobic microorganisms were determined on the hides of beef feedlot cattle before and after transport from the feedyard to the harvest facility in clean and dirty trailers. Swab samples were taken from the midline and withers of 40 animals on each of 8 days before and after shipping. After samples were collected, animals were loaded in groups of 10 on upper and lower levels of clean and dirty trailers. Animals were unloaded at the harvest facility and kept in treatment groups for sample collection after exsanguination. Salmonella was found more often on hide swabs collected from the midline than on than samples collected from the withers from animals transported in both clean and dirty trailers. Salmonella was found on significantly more hide swabs collected at harvest from both sampling locations than on those collected at the feedyard, with no differences attributed to the type of trailer. At the feedyard, clean trucks had a lower percentage of Salmonella-positive samples than did dirty trucks before animals were loaded. However, after transport, both clean and dirty trucks had a similar prevalence of Salmonella. There were no differences in Salmonella prevalence on hides collected from animals transported on the top and bottom levels of clean and dirty trucks. E. coli O157:H7 was detected on less than 2% of the samples; therefore, no practical conclusions about prevalence could be drawn. Hides sampled at harvest had higher concentrations of aerobic microorganisms than did hides sampled at the feedyard, and concentrations were higher on the midline than on the withers. Although the prevalences of Salmonella and total aerobic microorganisms increased on hides after transport from the feedyard to the plant, this increase was not related to the cleanliness of the trailers or the location of the cattle in the trailers.

Effect of method of blood sample collection on adrenal activity in farmed red deer and sheep following administration of ACTH

1998 ◽  
Vol 67 (1) ◽  
pp. 157-164 ◽  
Author(s):  
I. Ferre ◽  
P. J. Goddard ◽  
A. J. Macdonald ◽  
C. A. Littlewood ◽  
E. I. Duff
Keyword(s):  
Blood Sample ◽  
Plasma Cortisol ◽  
Sampling Method ◽  
Red Deer ◽  
Blood Sampling ◽  
Domestic Sheep ◽  
Sample Collection ◽  
Acth Stimulation ◽  
Treatment Groups ◽  
Stimulation Tests

AbstractThe effect of method of blood sample collection (automatic blood sampling equipment (ABSE) v. manual) on cortisol and progesterone concentrations was investigated in 20 farmed red deer hinds and 20 domestic sheep ewes following dexamethasone and exogenous ACTH administration. Ten animals were subjected to either automatic sampling or manual sampling via jugular venipuncture in 1 week, with the treatment groups reversed in the 2nd week. The ABSE was programmed to collect a blood sample, then deliver 2 mg dexamethasone, collect a further blood sample 120 min later and then inject 100 fig ACTH. Thereafter, samples were collected at 15-min intervals during a 2·5 h period (12 samples in total). In the manual injection and sampling treatment, four samples were collected: (1) before dexamethasone administration, (2) before ACTH administration, (3) 60 min after ACTH administration, and (4) 150 min after ACTH administration. The success rate of blood sampling with ABSE was 80%. The overall mean packed cell volume (PCV) from samples collected by ABSE from both hinds and ewes was significantly lower than that from samples collected manually (P < 0·01) and PCV declined with time in manually sampled animals (P < 0·01). Plasma cortisol concentrations peaked at 45 min after ACTH administration in sheep and deer. In sheep, there was a marked fluctuation in the plasma cortisol concentrations with time. Both deer and sheep showed a reduced cortisol response to ACTH during week 2 irrespective of sampling method suggesting down-regulation of the response to ACTH. Maximum mean plasma progesterone concentration was reached at 15 to 30 min after ACTH administration. No significant differences in cortisol and progesterone responses due to blood sampling method were found in animals receiving prior dexamethasone treatment. This demonstrates that the ABSE has the ability to be used to effectively conduct ACTH stimulation tests without the need to handle the animals during the test.

scholarly journals The Intergenerational Impact of Genetic and Psychological Factors on Blood Pressure (InterGEN) Study

2016 ◽  
Vol 18 (5) ◽  
pp. 521-530 ◽  
Author(s):  
Jacquelyn Y. Taylor ◽  
Michelle L. Wright ◽  
Cindy A. Crusto ◽  
Yan V. Sun
Keyword(s):  
Blood Pressure ◽  
Psychological Factors ◽  
Saliva Sample ◽  
Design Procedure ◽  
Interaction Effects ◽  
Sample Collection ◽  
Weight Percentage ◽  
False Discovery ◽  
Gene Environment ◽  
Collection Tube

The Intergenerational Impact of Genetic and Psychological Factors on Blood Pressure (InterGEN) study aims to delineate the independent and interaction effects of genomic (genetic and epigenetic) and psychological–environmental (maternally perceived racial discrimination, mental health, and parenting behavior) factors on blood pressure (BP) among African American mother–child dyads over time. The purpose of this article is to describe the two-step genetic and epigenetic approach that will be executed to explore Gene × Environment interactions on BP using a longitudinal cohort design. Procedure for the single collection of DNA at Time 1 includes the use of the Oragene 500-format saliva sample collection tube, which provides enough DNA for both the Illumina Multi-Ethnic Genotyping and 850K EPIC methylation analyses. BP readings, height, weight, percentage of body fat, and percentage of body water will be measured on all participants every 6 months for 2 years for a total of 4 time points. Genomic data analyses to be completed include multivariate modeling, assessment of population admixture and structure, and extended analyses including Bonferroni correction, false discovery rate methods, Monte Carlo approach, EIGENSTRAT methods, and so on, to determine relationships among both main and interaction effects of genetic, epigenetic, and psychological environmental factors on BP.

scholarly journals Effect of snack eating on sensitive salivary stress markers cortisol and chromogranin A

2004 ◽  
Vol 9 (1) ◽  
pp. 27-29 ◽  
Author(s):  
Masahiro Toda ◽  
Kanehisa Morimoto ◽  
Shingo Nagasawa ◽  
Kazuyuki Kitamura
Keyword(s):  
Chromogranin A ◽  
Stress Markers

scholarly journals Xanthine Oxidase Inhibitor, Allopurinol, Prevented Oxidative Stress, Fibrosis, and Myocardial Damage in Isoproterenol Induced Aged Rats

2015 ◽  
Vol 2015 ◽  
pp. 1-9 ◽  
Author(s):  
Md. Abu Taher Sagor ◽  
Nabila Tabassum ◽  
Md. Abdullah Potol ◽  
Md. Ashraful Alam
Keyword(s):  
Oxidative Stress ◽  
Myocardial Infarction ◽  
Inflammatory Cells ◽  
Oxidase Inhibitor ◽  
Oxidative Stress Markers ◽  
Aged Rats ◽  
Sample Collection ◽  
Xanthine Oxidase Inhibitor ◽  
Glutathione Concentration ◽  
Stress Markers

We evaluated the preventive effect of allopurinol on isoproterenol (ISO) induced myocardial infarction in aged rats. Twelve- to fourteen-month-old male Long Evans rats were divided into three groups: control, ISO, and ISO + allopurinol. At the end of the study, all rats were sacrificed for blood and organ sample collection to evaluate biochemical parameters and oxidative stress markers analyses. Histopathological examinations were also conducted to assess inflammatory cell infiltration and fibrosis in heart and kidneys. Our investigation revealed that the levels of oxidative stress markers were significantly increased while the level of cellular antioxidants, catalase activity, and glutathione concentration in ISO induced rats decreased. Treatment with allopurinol to ISO induced rats prevented the elevated activities of AST, ALT, and ALP enzymes, and the levels of lipid peroxidation products and increased reduced glutathione concentration. ISO induced rats also showed massive inflammatory cells infiltration and fibrosis in heart and kidneys. Furthermore, allopurinol treatment prevented the inflammatory cells infiltration and fibrosis in ISO induced rats. In conclusion, the results of our study suggest that allopurinol treatment is capable of protecting heart of ISO induced myocardial infarction in rats probably by preventing oxidative stress, inflammation, and fibrosis.

Does Casein Phosphopeptid Amorphous Calcium Phosphate Provide Remineralization on White Spot Lesions and Inhibition of Streptococcus mutans?

2014 ◽  
Vol 38 (4) ◽  
pp. 302-306 ◽  
Author(s):  
Arzu Aykut-Yetkiner ◽  
Nazan Kara ◽  
Mustafa Ateş ◽  
Nazan Ersin ◽  
Fahinur Ertuğrul
Keyword(s):  
Calcium Phosphate ◽  
Streptococcus Mutans ◽  
Amorphous Calcium Phosphate ◽  
Saliva Sample ◽  
Test Group ◽  
White Spot ◽  
Control Group ◽  
White Spot Lesions ◽  
Sample Collection ◽  
Significant Difference

Objective: The aim of this study was to evaluate the remineralization effect of Casein Phosphopeptid Amorphous Calcium Phosphate (CPP-ACP) on white spot lesions (WSL) and its inhibitory effect on Streptococcus mutans colonization. Study design: The study group consisted of 60 children exhibiting at least 1-WSL. Subjects were randomly divided into 2 groups: a test group of using CPP-ACP cream (Tooth Mousse, GC Europe N.V., Leuven, Belgium) and a control group using only fluoride containing toothpaste for a period of 3-months. Baseline WSLs were scored using DIAGNOdent device (KaVo Germany) and the saliva samples were collected to measure S. mutans counts. After the 3-month period the WSLs were again recorded and the saliva sample collection was repeated. Wilcoxon Signed Ranks Test was used for statistical analysis. Results: DIAGNOdent measurements were increased by time (p=0.002) in control group and no statistically significant difference (p=0.217) was found in test group by the 3-month period. In both groups, the mutans counts were decreased in 3-month experimental period. Conclusions: These clinical and laboratory results suggested that CPP-ACP containing cream had a slight remineralization effect on the WSL in the 3-month evaluation period however longer observation is recommended to confirm whether the greater change in WSLs is maintained.

Application of the Model of Allostasis to Older Women’s Relocation to Senior Housing

2013 ◽  
Vol 16 (2) ◽  
pp. 197-208 ◽  
Author(s):  
Heidi H. Ewen ◽  
Jennifer Kinney
Keyword(s):  
Older Women ◽  
Allostatic Load ◽  
Stress Reactivity ◽  
Saliva Sample ◽  
Well Being ◽  
Lifestyle Changes ◽  
Sample Collection ◽  
Senior Housing ◽  
Detailed Picture ◽  
Analyze Data

Objectives:Adjustment to senior housing entails significant lifestyle changes and is a stressful process. The adaptation process is dynamic and has yet to be studied using the conceptual model of allostasis. This article presents exemplars of women whose profiles represent three allostatic states: successful adaptation (homeostasis), ongoing adaptation (allostasis), and maladaptation (allostatic load).Method:Older women who had relocated to senior housing participated in three interviews and monthly saliva sample collection over a 6-month period. Saliva was assayed for diurnal cortisol secretion. Triangulation of mixed methods was used to analyze data, and psychosocial data were mapped onto the cortisol graphs to illustrate changes in stress reactivity and well-being.Results:Coping abilities, perceptions of stressors, and cortisol measures provide a detailed picture of the interplay among events and perceptions and the effects of both on well-being.Discussion:The case exemplars provide detailed information on the complexity of psychosocial and physiological components of the model of allostasis. This study also fills a gap in knowledge on negative relocation outcomes using the allostatic model.

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