scholarly journals Antimicrobial Activity against Paenibacillus larvae and Functional Properties of Lactiplantibacillus plantarum Strains: Potential Benefits for Honeybee Health

Antibiotics ◽  
2020 ◽  
Vol 9 (8) ◽  
pp. 442 ◽  
Author(s):  
Massimo Iorizzo ◽  
Bruno Testa ◽  
Silvia Jane Lombardi ◽  
Sonia Ganassi ◽  
Mario Ianiro ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Biochemical Properties ◽  
Antagonistic Activity ◽  
Paenibacillus Larvae ◽  
Bacterial Disease ◽  
Osmotic Tolerance ◽  
Potential Benefits ◽  
Honeybee Health ◽  
Exopolysaccharides Production

Paenibacillus larvae is the causative agent of American foulbrood (AFB), a severe bacterial disease that affects larvae of honeybees. The present study evaluated, in vitro, antimicrobial activity of sixty-one Lactiplantibacillus plantarum strains, against P. larvae ATCC 9545. Five strains (P8, P25, P86, P95 and P100) that showed the greatest antagonism against P. larvae ATCC 9545 were selected for further physiological and biochemical characterizations. In particular, the hydrophobicity, auto-aggregation, exopolysaccharides production, osmotic tolerance, enzymatic activity and carbohydrate assimilation patterns were evaluated. The five L. plantarum selected strains showed suitable physical and biochemical properties for their use as probiotics in the honeybee diet. The selection and availability of new selected bacteria with good functional characteristics and with antagonistic activity against P. larvae opens up interesting perspectives for new biocontrol strategies of diseases such as AFB.

scholarly journals Pseudomonas sp. COW3 Produces New Bananamide-Type Cyclic Lipopeptides with Antimicrobial Activity against Pythium myriotylum and Pyricularia oryzae

Molecules ◽  
2019 ◽  
Vol 24 (22) ◽  
pp. 4170 ◽  
Author(s):  
Olumide Owolabi Omoboye ◽  
Niels Geudens ◽  
Matthieu Duban ◽  
Mickaël Chevalier ◽  
Christophe Flahaut ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Genome Mining ◽  
Antagonistic Activity ◽  
Pyricularia Oryzae ◽  
Pseudomonas Sp ◽  
Pythium Myriotylum ◽  
Cyclic Lipopeptides ◽  
Peptide Synthetase ◽  
Kendrick Mass Defect

Pseudomonas species are metabolically robust, with capacity to produce secondary metabolites including cyclic lipopeptides (CLPs). Herein we conducted a chemical analysis of a crude CLP extract from the cocoyam rhizosphere-derived biocontrol strain Pseudomonas sp. COW3. We performed in silico analyses on its whole genome, and conducted in vitro antagonistic assay using the strain and purified CLPs. Via LC-MS and NMR, we elucidated the structures of four novel members of the bananamide group, named bananamides D-G. Besides variability in fatty acid length, bananamides D-G differ from previously described bananamides A-C and MD-0066 by the presence of a serine and aspartic acid at position 6 and 2, respectively. In addition, bananamide G has valine instead of isoleucine at position 8. Kendrick mass defect (KMD) allowed the assignment of molecular formulae to bananamides D and E. We unraveled a non-ribosomal peptide synthetase cluster banA, banB and banC which encodes the novel bananamide derivatives. Furthermore, COW3 displayed antagonistic activity and mycophagy against Pythium myriotylum, while it mainly showed mycophagy on Pyricularia oryzae. Purified bananamides D-G inhibited the growth of P. myriotylum and P. oryzae and caused hyphal distortion. Our study shows the complementarity of chemical analyses and genome mining in the discovery and elucidation of novel CLPs. In addition, structurally diverse bananamides differ in their antimicrobial activity.

scholarly journals Antimicrobial activity of extracellular metabolites from antagonistic bacteria isolated from potato (Solanum phureja) crops

2014 ◽  
Vol 40 (3) ◽  
pp. 212-220 ◽  
Author(s):  
Sinar David Granada García ◽  
Antoni Rueda Lorza ◽  
Carlos Alberto Peláez
Keyword(s):  
Antimicrobial Activity ◽  
Cyclic Peptide ◽  
Biological Activities ◽  
Pathogenic Fungi ◽  
Antagonistic Activity ◽  
Solanum Phureja ◽  
Dual Culture ◽  
Crude Extracts ◽  
Extracellular Metabolites

Microorganisms for biological control are capable of producing active compounds that inhibit the development of phytopathogens, constituting a promising tool toob tain active principles that could replace synthetic pesticides. This study evaluatedtheability of severalpotentialbiocontrol microorganismsto produce active extracellular metabolites. In vitro antagonistic capability of 50 bacterial isolates from rhizospheric soils of "criolla" potato (Solanum phureja) was tested through dual culture in this plant with different plant pathogenic fungi and bacteria. Isolates that showed significantly higher antagonistic activity were fermented in liquid media and crude extracts from the supernatants had their biological activities assessed by optical density techniques. Inhibitory effecton tested pathogens was observed for concentrations between 0.5% and 1% of crude extracts. There was a correlation between the antimicrobial activity of extracts and the use of nutrient-rich media in bacteria fermentation. Using a bioguided method, a peptidic compound, active against Fusarium oxysporum, was obtained from the 7ANT04 strain (Pyrobaculum sp.). Analysis by nuclear magnetic resonance and liquid chromatography coupled to mass detector evidenced an 11-amino acid compound. Bioinformatic software using raw mass data confirmed the presence of a cyclic peptide conformed by 11 mostly non-standard amino acids.

scholarly journals Constituents and antimicrobial activity of the essential oils of six Himalayan Nepeta species

2010 ◽  
Vol 75 (6) ◽  
pp. 739-747 ◽  
Author(s):  
Dinesh Bisht ◽  
Rajendra Padalia ◽  
Lalit Singh ◽  
Veena Pande ◽  
Priyanka Lal ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Essential Oil ◽  
Essential Oils ◽  
Antagonistic Activity ◽  
Significant Activity ◽  
Variable Degree ◽  
Bacterial Strains ◽  
Fungal Strains ◽  
Microbial Strains

The essential oils from six Himalayan Nepeta species, viz. Nepeta leucophylla Benth., Nepeta discolor Royle ex Benth., Nepeta govaniana Benth., Nepeta clarkei Hook f., Nepeta elliptica Royle ex Benth. and Nepeta erecta Benth., were tested for their in vitro antimicrobial activity against six pathogenic bacterial and two fungal strains. The results showed that Pseudomonas aeruginosa was the most sensitive strain tested to the essential oils of Nepeta species. The essential oils of N. elliptica and N. erecta exhibited the highest activity against P. aeruginosa, followed by the essential oils of N. leucophylla and N. clarkei. The essential oils from N. elliptica and N. erecta were also found to be very effective against Serratia marcescens; while the essential oil from N. leucophylla displayed significant activity against Proteus vulgaris and Staphylococcus aureus. Other bacterial strains displayed variable degree of susceptibility against one or more of the tested essential oils. The essential oil from N. leucophylla also showed the highest antifungal activity against both tested fungal strains, viz. Candida albicans and Trichophyton rubrum, followed by the essential oils from N. clarkei, N. govaniana and N. erecta. Iridodial derivatives, viz. iridodial ?-monoenol acetate (25.4 %), dihydroiridodial diacetate (18.2 %) and iridodial dienol diacetate (7.8 %) were identified as the major constituents of N. leucophylla, while the essential oils from N. elliptica and N. erecta were dominated by (7R)-trans, trans nepetalactone (83.4 %) and isoiridomyrmecin (66.7 %), respectively. The essential oil of N. discolor was characterized by 1,8-cineole (25.5 %) and ?-caryophyllene (18.6 %), while N. clarkei was dominated by ?-sesquiphellandrene (22.0 %) and germacrene D (13.0 %). Isoiridomyrmecin (35.2 %) and pregeijerene (20.7 %) were identified as the major constituents of N. govaniana. In general the Nepeta species containing constituents with an iridoid or lactone skeleton were found to have the greater antagonistic activity against most of the microbial strains as compared to those containing regular terpene constituents.

scholarly journals In Vitro Antagonistic Effect of Gut Bacteriota Isolated from Indigenous Honey Bees and Essential Oils against Paenibacillus Larvae

2020 ◽  
Vol 21 (18) ◽  
pp. 6736 ◽  
Author(s):  
Miroslava Kačániová ◽  
Margarita Terentjeva ◽  
Jana Žiarovská ◽  
Przemysław Łukasz Kowalczewski
Keyword(s):  
Antimicrobial Activity ◽  
Essential Oils ◽  
Digestive Tract ◽  
Antagonistic Effect ◽  
Paenibacillus Larvae ◽  
Thymus Vulgaris ◽  
Plant Essential Oils ◽  
Microbial Groups ◽  
Selection Of

The aim of study was to isolate and identify the gut bacteria of Apis mellifera and to evaluate antagonistic effect of the bacteriota against Paenibacillus larvae, which causes American foulbrood (AFB) in honeybees. The dilution plating method was used for the quantification of selected microbial groups from digestive tract of bees, with an emphasis on the bacteriota of the bees’ intestines. Bacteria were identified using mass spectrometry (MALDI-TOF-MS Biotyper). Overall, five classes, 27 genera and 66 species of bacteria were identified. Genera Lactobacillus (10 species) and Bacillus (8 species) were the most abundant. Gram-negative bacteria were represented with 16 genera, whereas Gram-positive with 10 genera. Delftia acidovorans and Escherichia coli were the most abundant in the digestive tract of honey bee. Resistance to a selection of antimicrobials was assessed for the bacterial isolates from bee gut and confirmed against all antimicrobials included in the study, with the exception of cefepime. Lactobacillus spp., especially L. kunkeei, L. crispatus and L. acidophilus. showed the strongest antimicrobial activity against P. larvae, the causal pathogen of AFB. Antimicrobial activity of essential oils against isolated bacteria and two isolates of P. larvae were assessed. Application of a broad selection of plant essential oils indicated that Thymus vulgaris had the highest antimicrobial activity against P. larvae.

scholarly journals In vitro study of the antimicrobial activity of European propolis against Paenibacillus larvae

Apidologie ◽  
2017 ◽  
Vol 48 (3) ◽  
pp. 411-422 ◽  
Author(s):  
Valery A. Isidorov ◽  
Krzysztof Buczek ◽  
Grzegorz Zambrowski ◽  
Krzysztof Miastkowski ◽  
Izabela Swiecicka
Keyword(s):  
Antimicrobial Activity ◽  
In Vitro Study ◽  
Vitro Study ◽  
Paenibacillus Larvae

scholarly journals Paenibacillus larvae-Directed Bacteriophage HB10c2 and Its Application in American Foulbrood-Affected Honey Bee Larvae

2015 ◽  
Vol 81 (16) ◽  
pp. 5411-5419 ◽  
Author(s):  
Hannes Beims ◽  
Johannes Wittmann ◽  
Boyke Bunk ◽  
Cathrin Spröer ◽  
Christine Rohde ◽  
...  
Keyword(s):  
Honey Bee ◽  
Phage Therapy ◽  
Real Life ◽  
Antagonistic Activity ◽  
Bacterial Disease ◽  
American Foulbrood ◽  
Whole Genome Analysis ◽  
Content Type ◽  
The Family

ABSTRACTPaenibacilluslarvaeis the causative agent of American foulbrood (AFB), the most serious honey bee brood bacterial disease. We isolated and characterizedP. larvae-directed bacteriophages and developed criteria for safe phage therapy. Whole-genome analysis of a highly lytic virus of the familySiphoviridae(HB10c2) provided a detailed safety profile and uncovered its lysogenic nature and a putative beta-lactamase-like protein. To rate its antagonistic activity against the pathogens targeted and to specify potentially harmful effects on the bee population and the environment,P. larvaegenotypes ERIC I to IV, representatives of the bee gut microbiota, and a broad panel of members of the orderBacillaleswere analyzed for phage HB10c2-induced lysis. Breeding assays with infected bee larvae revealed that thein vitrophage activity observed was not predictive of the real-life scenario and therapeutic efficacy. On the basis of the disclosedP. larvae-bacteriophage coevolution, we discuss the future prospects of AFB phage therapy.

In vitro study of the antimicrobial activity of Brazilian propolis against Paenibacillus larvae

2008 ◽  
Vol 97 (3) ◽  
pp. 273-281 ◽  
Author(s):  
Esther Margarida A.F. Bastos ◽  
Michael Simone ◽  
Daniela Macedo Jorge ◽  
Ademilson Espencer Egea Soares ◽  
Marla Spivak
Keyword(s):  
Antimicrobial Activity ◽  
In Vitro Study ◽  
Vitro Study ◽  
Paenibacillus Larvae ◽  
Brazilian Propolis

scholarly journals Short communication: Antimicrobial activity of indoleacetic, gibberellic and coumaric acids against Paenibacillus larvae and its toxicity against Apis mellifera

2020 ◽  
Vol 18 (1) ◽  
pp. e05SC01
Author(s):  
Nicolás Szawarski ◽  
Pablo Giménez-Martínez ◽  
Giulia Mitton ◽  
Pedro Negri ◽  
Facundo Meroi Arcerito ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Apis Mellifera ◽  
Inhibitory Concentration ◽  
Clinical Symptoms ◽  
Paenibacillus Larvae ◽  
National University ◽  
Entre Rios ◽  
Mic Minimum Inhibitory Concentration ◽  
Honey Bee Larvae

Aim of study: To explore three isolated phytomolecules: indoleacetic acid (IAA), gibberellic acid (GA), and the secondary metabolite p-coumaric acid (CUM): (1) evaluating their toxicity against Apis mellifera larvae and adults under controlled conditions in the laboratory; (2) searching for antimicrobial activity against Paenibacillus larvae.Area of study: Honey bee larvae and adults were collected from the experimental apiary of the “Centro de Investigación en Abejas Sociales (CIAS)” (-37.9348798, -57.682817), Institute of the National University of Mar del Plata (UNMdP), Argentina.Material and methods: Paenibacillus larvae strains were isolated from beehives from different provinces of Argentina (Buenos Aires, Córdoba and Entre Ríos) showing clinical symptoms of the American foulbrood. All strains (S1, S2, S3, S4) were genotypically identified using PL5 and PL4 primers and characterized as genotype ERIC1. Then standard essays were performed to determined toxicity of phytomolecules in honey bees and antimicrobial activity through the broth microdilution method.Main results:  The diet with GA, IAA and CUM did not present toxic effects in larvae or adult bees, and only CUM showed antimicrobial activity against P. larvae. In this study, we obtained in vitro values of MNIC (minimum non-inhibitory concentration) of 500 μg mL-1 and a MIC (minimum inhibitory concentration) of 650 μg mL-1 for CUM.Research highlights: The obtained results remark its potential as a natural alternative for the control of P. larvae, avoiding the problems generated by the use of synthetic antibiotics such as the resistance phenomena and the contamination of hive’s products.

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