In vivo and in vitro antimicrobial activity of phytol, a diterpene molecule, isolated and characterized from Adhatoda vasica Nees. (Acanthaceae), to control severe bacterial disease of ornamental fish, Carassius auratus, caused by Bacillus licheniformis PKBMS16

2020 ◽  
Vol 141 ◽  
pp. 103977 ◽  
Author(s):  
Mandira Saha ◽  
P.K. Bandyopadhyay
Keyword(s):  
Antimicrobial Activity ◽  
Bacillus Licheniformis ◽  
Carassius Auratus ◽  
Ornamental Fish ◽  
Bacterial Disease ◽  
Adhatoda Vasica ◽  
In Vitro Antimicrobial Activity

scholarly journals Attenuation of Aeromonas hydrophila Infection in Carassius auratus by YtnP, a N-acyl Homoserine Lactonase from Bacillus licheniformis T-1

Antibiotics ◽  
2021 ◽  
Vol 10 (6) ◽  
pp. 631
Author(s):  
Mengfan Peng ◽  
Wentao Tong ◽  
Zhen Zhao ◽  
Ling Xiao ◽  
Zhaoyue Wang ◽  
...  
Keyword(s):  
Virulence Factors ◽  
Bacillus Licheniformis ◽  
Aeromonas Hydrophila ◽  
Biofilm Formation ◽  
Carassius Auratus ◽  
Quorum Quenching ◽  
Inhibition Rate ◽  
Sequence Identity

In this experiment, the quorum quenching gene ytnP of Bacillus licheniformis T-1 was cloned and expressed, and the effect against infection of Aeromonas hydrophila ATCC 7966 was evaluated in vitro and vivo. The BLAST results revealed a 99% sequence identity between the ytnP gene of T-1 and its homolog in B.subtilis sub sp. BSP1, and the dendroGram showed that the similarity in the YtnP protein in T-1 was 100% in comparison with B.subtilis 3610, which was categorized as the Aidc cluster of the MBL family. The AHL lactonase activity of the purified YtnP was detected as 1.097 ± 0.7 U/mL with C6-HSL as the substrate. Otherwise, purified YtnP protein could significantly inhibit the biofilm formation of A.hydrophila ATCC 7966 with an inhibition rate of 68%. The MIC of thiamphenicol and doxycycline hydrochloride against A. hydrophila reduced from 4 μg/mL and 0.5 μg/mL to 1 μg/mL and 0.125 μg/mL, respectively, in the presence of YtnP. In addition, YtnP significantly inhibited the expression of five virulence factors hem, ahyB, ast, ep, aerA of A. hydrophila ATCC 7966 as well (p < 0.05). The results of inhibition on virulence showed a time-dependence tendency, while the strongest anti-virulence effects were within 4–24 h. In vivo, when the YtnP protein was co-injected intraperitoneally with A. hydrophila ATCC 7966, it attenuated the pathogenicity of A. hydrophila and the accumulated mortality was 27 ± 4.14% at 96 h, which was significantly lower than the average mortality of 78 ± 2.57% of the Carassius auratus injected with 108 CFU/mL of A. hydrophila ATCC 7966 only (p < 0.001). In conclusion, the AHL lactonase in B. licheniformis T-1 was proven to be YtnP protein and could be developed into an agent against infection of A. hydrophila in aquaculture.

scholarly journals Wild Italian Prunus spinosa L. Fruit Exerts In Vitro Antimicrobial Activity and Protects Against In Vitro and In Vivo Oxidative Stress

Foods ◽  
2019 ◽  
Vol 9 (1) ◽  
pp. 5
Author(s):  
Luisa Pozzo ◽  
Rossella Russo ◽  
Stefania Frassinetti ◽  
Francesco Vizzarri ◽  
Július Árvay ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Antimicrobial Activity ◽  
Total Phenolic ◽  
Antioxidant Power ◽  
In Vitro Antimicrobial Activity ◽  
Food Industries ◽  
Gram Positive Bacteria ◽  
Prunus Spinosa

Polyphenol-rich foods could have a pivotal function in the prevention of oxidative stress-based pathologies and antibacterial action. The purpose of this study was to investigate the in vitro antimicrobial activity, as well as the in vitro and In Vivo antioxidant capacities of wild Prunus spinosa L. fruit (PSF) from the southeast regions of Italy. The total phenolic content (TPC) was quantified, and the single polyphenols were analyzed by HPLC-DAD, showing high rutin and 4-hydroxybenzoic acid levels, followed by gallic and trans-sinapic acids. PSF extract demonstrated antimicrobial activity against some potentially pathogenic Gram-negative and Gram-positive bacteria. Besides, we investigated the cellular antioxidant activity (CAA) and the hemolysis inhibition of PSF extract on human erythrocytes, evidencing both a good antioxidant power and a marked hemolysis inhibition. Furthermore, an In Vivo experiment with oxidative stress-induced rats treated with a high-fat diet (HFD) and a low dose of streptozotocin (STZ) demonstrated that PSF has a dose-dependent antioxidant capacity both in liver and in brain. In conclusion, the wild Italian Prunus spinosa L. fruit could be considered a potentially useful material for both nutraceutical and food industries because of its antioxidant and antimicrobial effects.

scholarly journals In Vitro Antimicrobial Activity of GSQ1530, a New Heteroaromatic Polycyclic Compound

2002 ◽  
Vol 46 (10) ◽  
pp. 3168-3174 ◽  
Author(s):  
Yigong Ge ◽  
Stacey Difuntorum ◽  
Sofia Touami ◽  
Ian Critchley ◽  
Roland Bürli ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Therapeutic Potential ◽  
Clinical Isolates ◽  
In Vivo Studies ◽  
Cross Resistance ◽  
Minimal Bactericidal Concentration ◽  
Gram Positive ◽  
In Vitro Antimicrobial Activity

ABSTRACT GSQ1530 is a compound derived from a newly identified class of antibiotics referred to as heteroaromatic polycyclic (HARP) antibiotics. The aim of this study was to assess the in vitro antimicrobial activity of GSQ1530. By using an NCCLS broth microdilution assay, the activities of GSQ1530 and other antibiotics were coevaluated against 215 clinical isolates. The MICs at which 90% of isolates are inhibited (MIC90s) of GSQ1530 for methicillin-susceptible Staphylococcus aureus and methicillin-resistant S. aureus (MRSA) were 2 and 4 μg/ml, respectively. The MIC90s of GSQ1530 for the streptococci tested were 2 μg/ml or less, regardless of their susceptibilities to other antibiotics. The MIC90 of GSQ1530 for the enterococci tested (including vancomycin-resistant enterococci) was 4 μg/ml. No cross-resistance was found between GSQ1530 and other known antibiotics. In a separate assay, GSQ1530 demonstrated excellent activity against vancomycin-intermediate-susceptible staphylococci (MIC90, 1 μg/ml). The minimal bactericidal concentration test was conducted with 73 clinical isolates; GSQ1530 was cidal against streptococci and staphylococci but static against enterococci. An in vitro killing kinetic study revealed a time-dependent profile, with at least a 3-log reduction of bacterial growth within 6 h after exposure to four times the MICs of GSQ1530 for both S. aureus and Streptococcus pneumoniae. The checkerboard study showed that GSQ1530 had a synergistic interaction with rifampin against MRSA. The test medium was found to have little effect on in vitro antimicrobial potency. The MICs of GSQ1530 for gram-positive cocci were 4- to 32-fold higher in the presence of serum proteins. GSQ1530 has high levels of plasma protein binding (91 and 89% for rat and human plasma, respectively). These preliminary results demonstrate that GSQ1530, a representative compound of our novel HARP antibiotics, has broad-spectrum activity against gram-positive bacteria. This novel class of antibacterial compounds is profiled in vivo to assess the therapeutic potential in humans. Ongoing in vivo studies will assess whether this class of molecules has promising in vivo efficacy and safety profiles.

scholarly journals In Vitro Antimicrobial Activity and Probiotic Potential of Bifidobacterium and Lactobacillus against Species of Clostridium

Nutrients ◽  
2019 ◽  
Vol 11 (2) ◽  
pp. 448 ◽  
Author(s):  
Cinara Monteiro ◽  
Monique do Carmo ◽  
Bruna Melo ◽  
Matheus Alves ◽  
Camilla dos Santos ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Gas Production ◽  
Host Cells ◽  
Clostridium Species ◽  
Adhesive Properties ◽  
In Vitro Antimicrobial Activity ◽  
Probiotic Potential ◽  
Susceptibility Profiles

Many Clostridium species are found as commensal members of the intestinal microbiota. However, imbalances of the microbiota may lead to certain infections caused by these microorganisms, mainly Clostridium butyricum, Clostridium difficile, and Clostridium perfringens. In many cases, infection recurrence can occur after antibiotics, indicating the need for novel therapeutic options that act on the pathogens and also restore the microbiota. Herein, the in vitro antimicrobial activity and probiotic potential of clinical and reference strains of Bifidobacterium and Lactobacillus were investigated against Clostridium species. Antimicrobial activity was evaluated by the agar spot test and inhibition of gas production. Then, the probiotic potential of selected strains was assessed by analyzing their coaggregation ability, adhesive properties to host cells and mucin, tolerance to acidic pH and bile salts, and antimicrobial susceptibility profiles. Lactobacillus plantarum ATCC 8014 was the most promising strain based on its inhibitory activity against Clostridium spp. Also, this strain met criteria to be considered a probiotic based on its coaggregation ability, adhesive properties, and tolerance to harsh pH and bile acid salt conditions. The results indicate that among the studied strains, L. plantarum ATCC 8014 presents probiotic potential for controlling infections induced by the studied Clostridium species and should be further evaluated in in vivo animal models.

In vitro antimicrobial activity of T-91825, a novel anti-MRSA cephalosporin, and in vivo anti-MRSA activity of its prodrug, TAK-599

2004 ◽  
Vol 10 (3) ◽  
pp. 146-156 ◽  
Author(s):  
Yuji Iizawa ◽  
Junko Nagai ◽  
Tomoyasu Ishikawa ◽  
Shohei Hashiguchi ◽  
Masafumi Nakao ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
In Vitro Antimicrobial Activity
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