Antioxidants Present in Reproductive Tract Fluids and Their Relevance for Fertility

Nowadays, infertility is classified as a disease of the reproductive system. Although it does not compromise the life of the individual, it can have detrimental effects on the physiological and psychological health of the couple. Male fertility evaluation is mainly focused on the analysis of sperm parameters. However, the ejaculated fluid is also composed of seminal plasma, and the study of this fluid can provide crucial information to help in the assessment of male fertility status. Total antioxidant capacity of the seminal plasma has been positively correlated with the fertility of men. Moreover, evidence highlights to a similar importance as that of female reproductive tract fluid antioxidant capabilities and female fertility. Herein, we describe the functions of seminal plasma and female reproductive tract fluids, as well as their main antioxidant components and their relationships with fertility outcomes. Additionally, this review contains the most up to date information regarding the mechanisms of the interaction between the male and the female reproductive fluids and the importance of proper antioxidant capacity for fertilization.

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The Vehicle Determines the Destination: The Significance of Seminal Plasma Factors for Male Fertility

International Journal of Molecular Sciences ◽

10.3390/ijms21228499 ◽

2020 ◽

Vol 21 (22) ◽

pp. 8499

Author(s):

Fengli Wang ◽

Weina Yang ◽

Sijin Ouyang ◽

Shuiqiao Yuan

Keyword(s):

Seminal Plasma ◽

Male Fertility ◽

Reproductive Tract ◽

Biological Fluid ◽

Female Reproductive Tract ◽

Molecular Structures ◽

Contributing Factors ◽

Non Invasive ◽

Long Time ◽

Plasma Factors

Of all human infertility cases, up to 50% show contributing factors leading to defects in the male reproductive physiology. Seminal plasma (SP) is the biological fluid derived from the male accessory sex gland which carries spermatozoa passing throughout the male and female reproductive tract during ejaculation. It contains a complicated set of heterogeneous molecular structures, including proteins, cell-free nucleic acid (DNA, microRNA and LncRNA), and small-molecule metabolites as well as inorganic chemicals (ions). For a long time, the substantial significance of seminal plasma factors’ functions has been underestimated, which is restricted to spermatozoa transport and protection. Notably, significant advancements have been made in dissecting seminal plasma components, revealing new insights into multiple aspects of sperm function, as well as fertilization and pregnancy outcomes in recent years. In this review, we summarize the state-of-art discoveries regarding SP compositions and their implications in male fertility, particularly describing the novel understanding of seminal plasma components and related modifications using “omics” approaches and mainly focusing on proteome and RNA-seq data in the latest decade. Meanwhile, we highlighted the proposed mechanism of the regulation of SP molecules on immunomodulation in the female reproductive tract. Moreover, we also discussed the proteins investigated as non-invasive diagnosis biomarkers for male infertility in the clinic.

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Zinc: A Necessary Ion for Mammalian Sperm Fertilization Competency

International Journal of Molecular Sciences ◽

10.3390/ijms19124097 ◽

2018 ◽

Vol 19 (12) ◽

pp. 4097 ◽

Cited By ~ 19

Author(s):

Karl Kerns ◽

Michal Zigo ◽

Peter Sutovsky

Keyword(s):

Male Fertility ◽

Reproductive Tract ◽

Zinc Supplementation ◽

Female Reproductive Tract ◽

Sperm Maturation ◽

Cellular Mechanisms ◽

Human Reproductive ◽

Semen Processing ◽

New Research ◽

And Function

The importance of zinc for male fertility only emerged recently, being propelled in part by consumer interest in nutritional supplements containing ionic trace minerals. Here, we review the properties, biological roles and cellular mechanisms that are relevant to zinc function in the male reproductive system, survey available peer-reviewed data on nutritional zinc supplementation for fertility improvement in livestock animals and infertility therapy in men, and discuss the recently discovered signaling pathways involving zinc in sperm maturation and fertilization. Emphasis is on the zinc-interacting sperm proteome and its involvement in the regulation of sperm structure and function, from spermatogenesis and epididymal sperm maturation to sperm interactions with the female reproductive tract, capacitation, fertilization, and embryo development. Merits of dietary zinc supplementation and zinc inclusion into semen processing media are considered with livestock artificial insemination (AI) and human assisted reproductive therapy (ART) in mind. Collectively, the currently available data underline the importance of zinc ions for male fertility, which could be harnessed to improve human reproductive health and reproductive efficiency in agriculturally important livestock species. Further research will advance the field of sperm and fertilization biology, provide new research tools, and ultimately optimize semen processing procedures for human infertility therapy and livestock AI.

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High total antioxidant capacity of the porcine seminal plasma (SP-TAC) relates to sperm survival and fertility

Scientific Reports ◽

10.1038/srep18538 ◽

2015 ◽

Vol 5 (1) ◽

Cited By ~ 27

Author(s):

Isabel Barranco ◽

Asta Tvarijonaviciute ◽

Cristina Perez-Patiño ◽

Inmaculada Parrilla ◽

Jose J. Ceron ◽

...

Keyword(s):

Antioxidant Capacity ◽

Seminal Plasma ◽

Total Antioxidant Capacity ◽

Total Antioxidant ◽

Sperm Survival

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Spermatozoa Obtained From Alpaca vas deferens. Effects of Seminal Plasma Added at Post-thawing

Frontiers in Veterinary Science ◽

10.3389/fvets.2021.611301 ◽

2021 ◽

Vol 8 ◽

Author(s):

Eduardo G. Aisen ◽

Wilfredo Huanca López ◽

Manuel G. Pérez Durand ◽

Edita Torres Mamani ◽

Juan C. Villanueva Mori ◽

...

Keyword(s):

Seminal Plasma ◽

Artificial Insemination ◽

Reproductive Tract ◽

Vas Deferens ◽

Female Reproductive Tract ◽

Low Fertility ◽

Sperm Cells ◽

South American Camelids ◽

Thawing Process

The viscous seminal plasma (SP) is currently a major impediment to the handling of ejaculate and the development of some biotechnologies in South American camelids. The vas deferens-collected spermatozoa of alpacas is a useful technique to avoid this problem. On the other hand, SP contains a large protein component that has been implicated in the function of spermatozoa within the female reproductive tract. In this sense, the low fertility achieved using transcervical insemination with frozen-thawed spermatozoa in alpacas could be improved by adding SP. This study aimed to evaluate the effect of the whole SP on some in vitro parameters of alpaca spermatozoa after the freezing-thawing-process and the fertility after artificial insemination. It would contribute to a better understanding of the interaction between thawed sperm cells and SP. Spermatozoa were obtained by surgically diverted vas deferens. The samples were diluted with a Tris-based extender, packaged in straws, and frozen. At thawing, each straw was divided into two post-thawing conditions: with the addition of 10% of PBS (control) or with 10% SP (treatment). The sperm cells were evaluated using dynamic parameters, sperm cell morphology, and morphometry. Fertility was assessed by an artificial insemination trial. All in vitro parameters were analyzed by ANOVA. A heterogeneity test was scheduled for the fertility trial. After the freezing-thawing process, motility and plasma membrane functionality was improved when SP was added. No differences were found for post-thaw viability between the control and treatment samples. The percentage of normal cells was higher with SP at post-thawing, and a decrease of the presence of bent tailed spermatozoa with a droplet in the SP group was observed. The length of the head spermatozoa was 3.4% higher in the samples with PBS compared to those in which SP was added. Females pregnant at day 25 post-insemination were 0/12 (with SP inside the straw) and 1/10 (without SP inside the straw). In conclusion, the presence of 10% SP at post-thawing improves sperm cells' motility, functionality, and morphology, indicating that it would be beneficial to improve the frozen-thawed alpaca's physiology spermatozoa. More fertility trials must be developed to increase this knowledge.

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Heparin- and Zn2+-binding proteins from boar seminal plasma.

Acta Biochimica Polonica ◽

10.18388/abp.1999_4116 ◽

1999 ◽

Vol 46 (4) ◽

pp. 935-939 ◽

Cited By ~ 5

Author(s):

D Hołody ◽

J Strzezek

Keyword(s):

Amino Acid ◽

Affinity Chromatography ◽

Molecular Mass ◽

Seminal Plasma ◽

Binding Proteins ◽

Reproductive Tract ◽

Female Reproductive Tract ◽

Heparin Binding ◽

Sds Page ◽

Protein Components

Low molecular mass, heparin-binding proteins from seminal plasma play an important role in gametes interaction whereas plasmatic Zn2+-binding proteins stabilize chromatin and plasmalemma structures and protect spermatozoa in the female reproductive tract. By means of affinity chromatography the heparin- and Zn2+-binding proteins were isolated from boar seminal plasma and both preparations were analyzed by reverse HPLC. Most of the proteins bound to heparine and Zn2+-ions were classified as spermadhesins. Three fractions binding exclusively Zn2+ were isolated. They differ in amino-acid composition, content of glucosamine and content of protein components revealed by SDS/PAGE.

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Seminal Plasma Triggers the Differential Expression of the Glucocorticoid Receptor (NR3C1/GR) in the Rabbit Reproductive Tract

Animals ◽

10.3390/ani10112158 ◽

2020 ◽

Vol 10 (11) ◽

pp. 2158

Author(s):

Mateo Ruiz-Conca ◽

Jaume Gardela ◽

Amaia Jauregi-Miguel ◽

Cristina A. Martinez ◽

Heriberto Rodríguez-Martinez ◽

...

Keyword(s):

Differential Expression ◽

Seminal Plasma ◽

Time Course ◽

Reproductive Tract ◽

Receptor Gene ◽

Early Embryo ◽

Female Reproductive Tract ◽

Relevant Role ◽

Morula Stage ◽

Embryo Transport

Rabbits are interesting as research animal models for reproduction, due to their condition of species of induced ovulation, with the release of endogenous gonadotropin-releasing hormone (GnRH) due to coitus. Glucocorticoid (GC) signaling, crucial for physiological homeostasis, is mediated through a yet unclear mechanism, by the GC receptor (NR3C1/GR). After mating, the female reproductive tract undergoes dynamic modifications, triggered by gene transcription, a pre-amble for fertilization and pregnancy. This study tested the hypothesis that when ovulation is induced, the expression of NR3C1 is influenced by sperm-free seminal plasma (SP), similarly to after mating (whole semen), along the different segments of the internal reproductive tract of female rabbits. Semen (mating) was compared to vaginal infusion of sperm-free SP (Experiment 1), and changes over time were also evaluated, i.e., 10, 24, 36, 68, and 72 h post-mating, corresponding to specific stages, i.e., ovulation, fertilization, and the interval of early embryo development up to the morula stage (Experiment 2). All does were treated with GnRH to induce ovulation. Samples were retrieved from seven segments of the reproductive tract (from the cervix to infundibulum), at 20 h post-mating or sperm-free SP infusion (Experiment 1) or at 10, 24, 36, 68, and 72 h post-mating (Experiment 2). Gene expression of NR3C1 was analyzed by qPCR. Results showed an increase in NR3C1 expression in the infundibulum compared to the other anatomical regions in the absence of spermatozoa when sperm-free SP infusion was performed (Experiment 1). Moreover, during the embryo transport through the oviduct, the distal isthmus was time-course upregulated, especially at 72 h, when morulae are retained in this anatomical region, while it was downregulated in the distal uterus at 68 h (Experiment 2). The overall results suggest that NR3C1, the GC receptor gene, assessed in the reproductive tract of does for the first time, shows differential expression changes during the interval of oviductal and uterine embryo transport that may imply a relevant role of the GC action, not only close to the site of ovulation and fertilization, but also in the endometrium.

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