scholarly journals Study of Antioxidant Activity of Some Medicinal Plants Having High Content of Caffeic Acid Derivatives

Antioxidants ◽  
2020 ◽  
Vol 9 (5) ◽  
pp. 412 ◽  
Author(s):  
Agnieszka Tajner-Czopek ◽  
Mateusz Gertchen ◽  
Elżbieta Rytel ◽  
Agnieszka Kita ◽  
Alicja Z. Kucharska ◽  
...  

Recently, there has been increasing interest in medicinal plants, due to their content of health-promoting compounds, e.g., caffeic acids derivatives. Hence, the aim of this work was to study the antioxidant activity of extracts obtained from the following medicinal plants: caraway (Carum carvi L.), coltsfoot (Tussilago farfara L.), dandelion (Taraxacum officinale F.H.Wigg.), lovage (Levisticum officinale L.), tarragon (Artemisia dracunculus L.) and white mulberry (Morus alba L.), characterized by their high content of caffeic acid derivatives. The water-ethanolic extracts were characterized on average by about 9 times higher contents of caffeic acid derivatives level than water extracts. Both in water and water-ethanolic extracts, the dominant phenolic acid was 5-CQA (5-O-caffeoylquinic acid) and 3,4-diCQA (3,4-dicaffeoylquinic acid), then CCA-1 (chicoric acid isomer 1), which appeared only in water-ethanolic extracts. Extracts from dandelion contained compounds such as CTA (caftaric acid), CCA-1 (chicoric acid isomer 1) and CCA-2 (chicoric acid isomer 2), which were not detected in other plant extracts examined in this work. The water-ethanolic extracts from coltsfoot and tarragon were characterized by a high content of di-caffeoylquinic acids, especially 3,4-diCQA and 3,5-diCQA, respectively. It has been stated that there is a positive correlation between caffeic acid derivatives and antioxidant activity (radical cation scavenging activity (ABTS) and radical scavenging activity (DPPH)), especially in water-ethanolic extract of medicinal plants.

2014 ◽  
Vol 68 (1) ◽  
pp. 43-49 ◽  
Author(s):  
Violeta Jakovljevic ◽  
Jasmina Milicevic ◽  
Jelica Stojanovic ◽  
Slavica Solujic ◽  
Miroslav Vrvic

The aim of this study was to investigate the biological and chemical activity on two species of fungi of the genus Penicillium isolated from wastewater. On the selected species of fungi the different antioxidant activity assays were carried out: DPPH free-radical scavenging activity, total antioxidant activity, Fe2+- chelating ability and Fe3+- reducing power. Total phenol content was also determinate for ethanolic extract of mycelia. Penicillium chrysogenum ethanolic extract contained higher total phenolic content and better total antioxidant capacity as well as ferrous ion chelating ability. Penicillium fumiculosum ethanolic extract showed higher DPPH free-radical scavenging activity, as well as reducing power. Based on the obtained results it can be concluded that two types of fungi are potential new sources of natural antioxidants.


2021 ◽  
Vol 119 ◽  
pp. 04001
Author(s):  
Khaled Abdu ◽  
Rahma Erahioui ◽  
Khadija Khedid ◽  
Hefdhal deen ◽  
Maha Elhawary ◽  
...  

This paper presents a comparative study about the evaluation of antioxidant activity and corrosion inhibition in Kenitra, Marrakesh and Taiz. An interesting topic, indeed polyphenols can improve or help to reduce oxidative stress in the treatment of digestive problems, weight management difficulties, diabetes, hypertension, arteriosclerosis and damage fatty tissue. Therefore, the authors deal with the measurement of polyphenol content and evaluation of the antioxidant activity of lemon peels in Kenitra , Marrakesh and Taiz.The authors performs empirical analyzes on lemon peels. The total polyphenol contents of the ethanolic extract of lemon peels were measured. It was to be 30. 23, 26. 346 and 20.961 mg/CE/g in Kenitra, Marrakech, and Taiz, respectively. Moreover, the DPPH radical scavenging activity of ethanolic extract of dried lemon peels was higher than 200μg/ml concentration. They were 73.47%, 47.36%, and 32.09in Kenitra, Marrakech, and Taiz, respectively. Also, the IC50 values of ethanolic extracts calculated from the percentage inhibitions at the same concentration. Inhibition (IC50) which obtained in Kenitra was 123.089 μg/ml. It was lower compared to the ethanol extract of Marrakech and Taiz that were 197.418, 276.750μg/mL, respectively. Therefore, the extract which is containing a high amount of phenolic is showed high radical scavenging activity. In addition, the maximum inhibition efficiencies for 2 mL L-1 of the inhibitor at 298 k were 98, 12% and 84, 85 % in Kenitra and Taiz, respectively. These values obtained through polarization curve measurements.


Author(s):  
Rasna Gupta ◽  
Ram Lakhan Singh ◽  
Neeraja Dwivedi

Objective: To investigate the antioxidant activity of ethanolic extract of Terminalia bellerica fruit pulp (TBFP), seed (TBS) and bark (TBB), and identification of phytochemical constituents of said extracts.Methods: The antioxidant potential of T. bellerica (TB) parts was evaluated by free radical scavenging activity (FRSA), superoxide anion radical scavenging activity (SARSA), hydroxyl radical scavenging activity (HRSA) and compared with reference standard quercetin. Lipid peroxidation (LPO), ferric thiocyanate activity (FTC) and reducing power (RP) of the plant extracts were also examined. The phytochemical constituents of said extracts have been quantified by gas chromatography-mass spectrometer (GC-MS) method.Results: Ethanolic extract of TBFP exhibited high phenolic content (254.33) followed by TBS (227.33) and TBB (185) mg/g of GAE. TBFP showed lowest IC50 for FRSA (62 µg/ml), SARSA (39 µg/ml) and HRSA (27 µg/ml) and highest RP (3.39 ascorbic acid equivalents/ml). TBB showed lowest IC50 for LPO (48 µg/ml) whereas TBS showed lowest IC50 for FTC (109 µg/ml). Predominant phyto-compounds present in TB extracts were quinic acid, gallic acid, ethyl galate, 9, 12 octadecadienoic acid and glucopyranose in varying concentrations as analyzed by GC-MS.Conclusion: On the basis of correlation of antioxidant studies with total phenolic content (TPC) and GC-MS analysis of different part of TB, it may be concluded that high concentration of total phenolics and other bioactive phytochemicals of TB extracts make it a potential source of nutraceutical antioxidants.


2021 ◽  
Vol 57 (2) ◽  
pp. 76-83
Author(s):  
Mihai TODORAN ◽  
◽  
Liana HANGA ◽  
Daniela BENEDEC ◽  
◽  
...  

Objectives. The present analysis was to investigate the content of caffeic acid derivatives, antioxidant activity, and the presence of caffeic acid in alcoholic extracts obtained from 16 simple teas from the following medicinal plants: Vaccinium mуrtilluѕ, Camеllia ѕinеnѕiѕ, Coffea arabica, Mеlіssa οffіcіnalіs, Οcіmum basіlіcum, Rοsmarіnus οffіcіnalіs, Salvіa οffіcіnalіs, Ηyssοрus οffіcіnalіs, Αrtеmіsіa absіntһіum, Cynara scοlymus, Calendula officinalis, Cοrіandrum satіvum, Fοеnіculum vulgarе, Carum carvі, Rοsa canіna, Crataеgus mοnοgyna. Materials and methods. The presence of caffeic acid was evaluated using the thin layer chromatography method (TLC). The total content of phenylpropanoids from the medicinal herbs alcoholic extracts was determined using a spectrophotometric method. Outcomes. Results were in the range of 0.046-2.426% caffeic acid derivatives (CAE%). The antioxidant properties were measured using the DPPH radical scavenging mechanism. The results were found to be in the range of 63.93% and 0.38% inhibition level (I%). The greatest antioxidant activity was measured for Camellia sinensis (I = 45.54%), followed by Vaccinium myrtillus (I = 50.289%). Conclusions. In this paper it was shown that these medicinal plants are rich in caffeic acid derivatives with antioxidant action capitalized in the prevention of serious diseases.


2018 ◽  
Vol 23 (3) ◽  
pp. 79
Author(s):  
Andayana Puspitasari Gani ◽  
Suwidjiyo Pramono ◽  
Sudibyo Martono ◽  
Sitarina Widyarini

Research of radical scavenging activity of sambiloto (Andrographis paniculata Nees) and patikan kebo (Euphorbia hirta L) ethanolic extracts and their combination toward 2,2-Diphenyl-1-Picrylhidrazyl (DPPH) has conducted. Subjects of this experiment comprise sambiloto ethanolic extract, patikan kebo ethanolic extract, dechlorophyllated extract of sambiloto and combinations among those three. Dechlorophyllation by electrocoagulation method is done to reduce chlorophyll content thereby increasing the level of active compound in the sambiloto extract. The results of dechlorophyllation showed that only total flavonoid content in the extract of sambiloto increased, meanwhile the radical scavenging activity results showed that patikan kebo ethanolic extract has the lowest IC50 value (22,36 µg/mL) compared to sambiloto ethanolic extract (IC50 value 499,03 µg/mL). Extract combination may increase DPPH radical scavenging activity of the sambiloto extract (IC50 value 76,06 µg/mL) but not higher than that of patikan kebo single extract.


2014 ◽  
Vol 31 (4) ◽  
pp. 245-252 ◽  
Author(s):  
Jovana Veljković ◽  
Jelena Brcanović ◽  
Aleksandra Pavlović ◽  
Snežana Mitić ◽  
Biljana Kaličanin ◽  
...  

Summary While there is a large number of scientific papers reporting chemical composition and biological activities of Aronia melanocarpa, there is a lack information regarding the commercially available bagged tea. In order to supply new information on the antioxidant activity of the Aronia melanocarpa tea infusions, the aim of this study was to evaluate individual phenolic compounds which could be responsible for antioxidant activities of these beverages. Selected anthocyanins (cyanidin-3-O-galactoside, cyanidin-3-O-glucoside, cyanidin- 3-O-arabinoside, and cyanidin-3-O-xyloside), gallic acid, caffeic acid, rutin, morin, and protocatechuic acid were simultaneously detected from commercially available tea infusions using a High Performance Liquid Chromatographic (HPLC) method. The antioxidant activity was measured using five in vitro spectrophotometric methods: 1,1-diphenyl- 2-picrylhydrazyl radical scavenging activity (DPPH), 2,2'-azino-bis (3-ethylbenzthiazoline- 6-sulphonic acid) radical cation scavenging activity (ABTS), ferric reducing-antioxidant power (FRAP) and reduction power (RP)Fe(III) to Fe(II). Obtained results showed that anthocyanins, predominantly of cyanidin-3-O-galactoside, are the major class of polyphenolic compounds in tea infusions. Among phenolic acids the most abundant is caffeic acid. A significant correlation between DPPH and ABTS and FRAP and RP suggested that antioxidant components in these beverages were capable scavenging free radicals and reducing oxidants. Generally, these beverages had relatively high antioxidant capacities and could be important dietary sources of antioxidant phenolics for the prevention of diseases caused by oxidative stress.


Author(s):  
Carole Nweze Chibuzo ◽  
Abdurrasheed Yusuf Hauwa ◽  
Ahmed Zubairu

This study investigated the phytochemical composition andin-vitroradical scavenging activity of ethanolic extracts of Nutraceutical-C24/7 andLandolphialanceolatafruit. Phytochemical screening ofLandolphialanceolatafruit extractindicated the presence of phenols, flavonoids, saponins, alkaloids, terpenoids, tannins, triterpenoids and steroids. While nutraceutical-C24/7 showed precence of with exception of saponins, terpenoids and triterpenoids. Some of the identified Phytochemicals and vitamins concentrations quantified were higher inLandolphialanceolatafruit extractthan nutraceutical-C24/7 with statistically significant difference (p<0.05) with the former having flavonoids 0.062±0.00mg/ml, phenol 0.097±0.00mg/ml, vitamin C 206±42mg/dL, vitamin B154.16±0.00mg/dL and vitamin B20.11±0.00mg/dL while, Nutraceutical-C24/7 extract had flavonoid 0.117±0.00mg/ml, phenol 0.032±0.00, vitamin C 102.24±0.00mg/dL, vitamin B123.36±0.00mg/dL, and vitamin B20.067±0.00mg/dL. The study also showed that at higher concentration, DPPH radical, ferric ion, and hydrogen peroxide the extracts radical scavenging activity increased. The study showed that the extracts scavenging activities at different concentrations had higher percentage inhibition on DPPH radical, reducing power of ferric ion capabilities and reducing composition of hydrogen peroxide indicated that ethanolic extract ofLandolphialanceolatafruits showed statistically significant different (p<0.05) when compared with ethanolic extract of Nutraceutical-C24/7. Standard vitamin C percentage inhibition for DPPH radical compared well with that ofLandolphialanceolatafruit extract at higher concentration with no statistical significant different (p>0.05).The indigenous functional foodLandolphialanceolatafruit can be concluded to be a good antioxidant that can scavenge, inhibit and quench free radicals. This suggests thatLandolphialanceolatamay be considered a good source and alternative antioxidant for developing countries like Nigeria.


Author(s):  
Gamal A. Gabr ◽  
Salwa M. El-Sayed ◽  
Mohamed S. Hikal

Background and Objectives: The cyanobacterium Spirulina also called blue green algae is a class of gram negative bacteria which possesses wide range of bioactive colored components as Phycocyanin, carotenoids and chlorophyll. Spirulina is one of the microalgae containing nutrients that have been used as a functional food in addition to therapeutic and pharmaceutical applications. This study aimed to evaluate the biochemical composition of Spirulina platensis biomass and its ethanolic and aqueous extracts, as well as, evaluate the antioxidant activities of the biomass, ethanolic, aqueous and the purified Phycocyanin. Materials and Methods: The chemical compositions of Spirulina platensis were determined, as well as the antioxidant activity of extracts, Phycocyanin, Phycocyanopeptide and Phycocyanobilin using (DPPH) radical-scavenging activity. Results: Results show that biomass has higher total proteins (49.72±0.508%), total carbohydrates (10.3±0.330%), moisture content (7.5±0.685%), lipids (7.2±0.105%) and Minerals (6.9±0.130%). In biomass, the total phenols (51.20±0.25 µg/mL) and flavonoids (97.73±1.858 µg/mL) were high compared to the ethanolic (49.48±0.130 and 69.07±1.814 µg/mL) and aqueous (15.27±0.639 and 4.67±0.611 µg/mL) extracts respectively. In the phenolic compounds, pyrogallol was identified as the major compound in biomass and aqueous extract (63.85 and 12.33%) respectively, E-vanillic acid in ethanolic (18.20 %), whereas, hespirdin (3.517 and1.639%) were major flavonoids found in aqueous and ethanolic extracts respectively. The DPPH scavenging activity was found higher in ethanolic extract compared to aqueous while in bioactive, the order of antioxidant activity was Phycocyanin > Phycocyanobilin > Phycocyanopeptide. Conclusions: The study data regarding to Spirulina nutritional value, makes Spirulina an excellent choice when formulating diets and combating malnutrition. Furthermore, it is a strong antioxidant and could be used as alternative treatments as anticancer, antidiabetic, and anti-inflammatory agent.


Author(s):  
S. Srividya ◽  
G. Sridevi ◽  
A. G. Manimegalai

The ethanolic extract of the leaves of Cassia occidentalis (Co) were subjected to phytochemical analysis by standard qualitative analysis and the invitro antioxidant activity was evaluated by determination of total antioxidant capacity, 1.1- diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activity, hydrogen peroxide (H2O2) radical scavenging activity, superoxide scavenging activity and Ferric reducing anti oxidant potential (FRAP). The analyses revealed that the ethanolic extract of Co was able to efficiently scavenge the free radicals in a dose dependant manner. The results were compared with the standard antioxidant ascorbic acid. The results have shown that crude ethanolic extract of the leaves of Co showed excellent antioxidant activity due to the presence of bioactive compounds namely alkaloids, betacyanin, cardiac glycosides, coumarins, flavonoids, phenols, steroids, saponins, tannins, terpenoids, anthraquinones and emodins.


2010 ◽  
Vol 5 (1) ◽  
pp. 1934578X1000500
Author(s):  
Yu-Tang Tung ◽  
Shang-Tzen Chang

The antioxidant activity of extracts of Acacia confusa of different ages has been evaluated. Ethanolic extracts of bark, twigs, branches (2- and 5 cm-diameter branch-bark, 2- and 5 cm-diameter branch-wood), and leaves of 15-, 25-, and 35-year-old A. confusa were examined. Results indicate that the ethanolic extracts of 5 cm-diameter branch-bark and bark of A. confusa exhibited stronger antioxidant activities, and higher phenolic and proanthocyanidin contents than the other plant parts. Furthermore, twig, 2 cm-diameter branch-wood, 2 cm-diameter branch-bark, and 5 cm-diameter branch-wood extracts of 35-year-old specimens showed better antioxidant activities and higher phenolic contents, followed by the 25- and 15-year-old specimens tested. This reveals that the composition of extracts and their differential antioxidant activities are dependent on the age of A. confusa. In addition, the total phenolic content of A. confusa ethanolic extracts has a high positive correlation with the DPPH free-radical scavenging activity (R2 = 0.83), superoxide radical-scavenging activity (R2 = 0.66), ferrous ion-chelating effect (R2 = 0.67), and reducing power (R2 = 0.92).


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