Non-Thermal Atmospheric Pressure Plasma-Conditioned Root Dentin Promotes Attraction and Attachment of Primary Human Dental Pulp Stem Cells in Real-Time Ex Vivo

This study investigated if non-thermal atmospheric pressure plasma (NTAPP) treatment of root dentin surfaces promotes human dental pulp stem cell (hDPSCs) adhesion. Freshly extracted human single-rooted teeth (n = 36) were decoronated and cut (first vertically, then horizontally) into root dentin slices (3 mm thick). Primary hDPSCs cultures were seeded onto slices randomly assigned to pretreatment groups (n = 9/group): NaOCl (1.5%), EDTA (17%) then NTAPP (Group I); NaOCl then NTAPP (Group II); NaOCl then EDTA (Group III); and NaOCl alone (Group IV). Cell viability and proliferation were measured using MTT assay with log-linear statistical analysis. Cell attachment and spreading morphologies on dentin slices (n = 3/group) were examined through scanning electron microscopy. Early cell adhesion events and subcellular activities were observed in real time by live-cell imaging through holotomographic microscopy. Cell viability and proliferation were significantly higher on NTAPP-treated dentin (p < 0.05), without interactions with EDTA (p > 0.05). The attachment, spreading, extensions and multiple layers of hDPSCs were heightened on NTAPP-treated dentin. Cell adhesion, spreading, and dentinal tubule penetration were hastened on NTAPP-treated dentin surfaces in real-time, with elevated subcellular activities and intracellular lipid droplet formation. NTAPP-treated root dentin surfaces support enhanced cellular responses, potentially promoting pulp-dentin regeneration.

Download Full-text

Production of intracellular reactive oxygen species and change of cell viability induced by atmospheric pressure plasma in normal and cancer cells

Applied Physics Letters ◽

10.1063/1.4824986 ◽

2013 ◽

Vol 103 (15) ◽

pp. 153705 ◽

Cited By ~ 52

Author(s):

Sun Ja Kim ◽

Hea Min Joh ◽

T. H. Chung

Keyword(s):

Reactive Oxygen Species ◽

Cell Viability ◽

Cancer Cells ◽

Atmospheric Pressure ◽

Reactive Oxygen ◽

Atmospheric Pressure Plasma ◽

Intracellular Reactive Oxygen Species ◽

Oxygen Species ◽

Pressure Plasma

Download Full-text

Non-thermal atmospheric-pressure plasma can influence cell adhesion molecules on HaCaT-keratinocytes

Experimental Dermatology ◽

10.1111/j.1600-0625.2010.01159.x ◽

2010 ◽

Vol 20 (3) ◽

pp. 282-284 ◽

Cited By ~ 40

Author(s):

Beate Haertel ◽

Kristian Wende ◽

Thomas von Woedtke ◽

Klaus Dieter Weltmann ◽

Ulrike Lindequist

Keyword(s):

Cell Adhesion ◽

Adhesion Molecules ◽

Atmospheric Pressure ◽

Cell Adhesion Molecules ◽

Atmospheric Pressure Plasma ◽

Hacat Keratinocytes ◽

Pressure Plasma

Download Full-text

Hydrophilic surface modification of coronary stent using an atmospheric pressure plasma jet for endothelialization

Journal of Biomaterials Applications ◽

10.1177/0885328217748465 ◽

2017 ◽

Vol 32 (8) ◽

pp. 1083-1089 ◽

Cited By ~ 2

Author(s):

Jae Won Shim ◽

In-Ho Bae ◽

Dae Sung Park ◽

So-Youn Lee ◽

Eun-Jae Jang ◽

...

Keyword(s):

Contact Angle ◽

Cell Adhesion ◽

Endothelial Cell ◽

Atmospheric Pressure ◽

Atmospheric Pressure Plasma ◽

Plasma Jet ◽

Treated Group ◽

Endothelial Cell Migration ◽

Atmospheric Pressure Plasma Jet ◽

Pressure Plasma

The first two authors contributed equally to this study. Bioactivity and cell adhesion properties are major factors for fabricating medical devices such as coronary stents. The aim of this study was to evaluate the advantages of atmospheric-pressure plasma jet in enhancing the biocompatibility and endothelial cell-favorites. The experimental objects were divided into before and after atmospheric-pressure plasma jet treatment with the ratio of nitrogen:argon = 3:1, which is similar to air. The treated surfaces were basically characterized by means of a contact angle analyzer for the activation property on their surfaces. The effect of atmospheric-pressure plasma jet on cellular response was examined by endothelial cell adhesion and XTT analysis. It was difficult to detect any changeable morphology after atmospheric-pressure plasma jet treatment on the surface. The roughness was increased after atmospheric-pressure plasma jet treatment compared to nonatmospheric-pressure plasma jet treatment (86.781 and 7.964 nm, respectively). The X-ray photoelectron spectroscopy results showed that the surface concentration of the C–O groups increased slightly from 6% to 8% after plasma activation. The contact angle dramatically decreased in the atmospheric-pressure plasma jet treated group (22.6 ± 15.26°) compared to the nonatmospheric-pressure plasma jet treated group (72.4 ± 15.26°) ( n = 10, p < 0.05). The effect of the increment in hydrophilicity due to the atmospheric-pressure plasma jet on endothelial cell migration and proliferation was 85.2% ± 12.01% and 34.2% ± 2.68%, respectively, at 7 days, compared to the nonatmospheric-pressure plasma jet treated group (58.2% ± 11.44% in migration, n = 10, p < 0.05). Taken together, the stent surface could easily obtain a hydrophilic property by the atmospheric-pressure plasma jet method. Moreover, the atmospheric-pressure plasma jet might affect re-endothelialization after stenting.

Download Full-text