scholarly journals Changes in Elderberry (Sambucus nigra L.) Juice Concentrate Polyphenols during Storage

2021 ◽  
Vol 11 (15) ◽  
pp. 6941
Author(s):  
Cláudia M. B. Neves ◽  
António Pinto ◽  
Fernando Gonçalves ◽  
Dulcineia F. Wessel
Keyword(s):  
Phenolic Compounds ◽  
Chlorogenic Acid ◽  
Total Phenolic Content ◽  
Room Temperature ◽  
Phenolic Content ◽  
Total Phenolic ◽  
Sambucus Nigra ◽  
Juice Concentrate ◽  
Rutin And Quercetin ◽  
Polymeric Color

Elderberry (Sambucus nigra L.) juice concentrate is highly rich in polyphenols, particularly anthocyanins and flavonols, which have been associated with a wide range of health-promoting properties. Phenolic compounds, in particular anthocyanins, are unstable and may change during storage, which might influence the product color quality and its potential health effects. The aim of this study was to evaluate the changes in the polyphenols profile of elderberry juice concentrate produced at an industrial scale during seven months of storage at 5 °C and at room temperature. The total phenolic content, the total monomeric anthocyanins, the percent polymeric color, and the ABTS•+ scavenging activity were monitored over time. In addition, the profile and content of the main individual phenolic compounds were also assessed by HPLC-DAD. The results show that cyanidin-3-O-sambubioside, cyanidin-3-O-glucoside, cyanidin-3-O-sambubioside-5-O-glucoside, cyanidin-3,5-O-diglucoside, chlorogenic acid, rutin, and quercetin-3-O-glucoside were the main phenolic compounds identified. Storage at room temperature resulted in a strong reduction in total monomeric anthocyanin content accompanied by an increase in percent polymeric color values. Cyanidin-3-O-sambubioside and cyanidin-3-O-glucoside degraded faster than cyanidin-3,5-O-diglucoside and cyanidin-3-O-sambubioside-5-O-glucoside. Concentration of chlorogenic acid also decreased over storage, whereas rutin and quercetin-3-O-glucoside were quite stable. Storage at 5 °C caused a lower impact on the contents of anthocyanins and chlorogenic acid and the percent polymeric color was not affected. The total phenolic content and the in vitro antioxidant activity remained quite similar over the time, for both temperatures, suggesting that elderberry concentrates still preserve their health benefits of antioxidant capacity after seven months of storage.

scholarly journals Variety and Year Impact on Phenolic Content of Arkansas-Grown Sweetpotato Leaves

2020 ◽  
Vol 4 (Supplement_2) ◽  
pp. 371-371
Author(s):  
Josue Bolanos ◽  
Sun-Ok Lee ◽  
Luke Howard ◽  
Cindi Brownmiller ◽  
Shahidul Islam ◽  
...  
Keyword(s):  
Phenolic Compounds ◽  
Antioxidant Capacity ◽  
Chlorogenic Acid ◽  
Total Phenolic Content ◽  
Phenolic Content ◽  
Statistical Significance ◽  
Radical Scavenging ◽  
Dry Weight ◽  
Antioxidant Potential ◽  
Total Phenolic

Abstract Objectives Sweetpotato leaf (SPL) is a natural source of phenolic compounds with potential utility as an antioxidant. The study aimed to measure the impacts of the years on SPL total phenolic content and antioxidant capacity and to identify and quantify the individual phenolic compounds. Methods Sweetpotato leaves in 2018 (27 varieties) and 2019 (24 varieties) were grinded and lyophilized. Phenolic compounds were extracted with 70% ethanol from SPLs. Total phenolic content was determined by Folin-Ciocalteu method and antioxidant potential was determined by DPPH (2,2-diphenyl-1-picrylhydrazyl) radical scavenging assay. Five SPL samples with statistically highest antioxidant capacity were identified and quantified by HPLC. All statistical analyses were carried out by SAS software using ANOVA. Statistical significance was accepted at P < 0.05. Results The average of total phenolic contents in 2018 SPL samples was 43 ± 13 mg gallic acid equivalent (GAE)/g dry weight (DW) SPL whereas in 2019, it was 53 ± 9 mg GAE/g dry weight SPL. Antioxidant capacity in 2018 was 110 ± 75 µmol Trolox equivalent (TE)/g dry weight SPL whereas in 2019, it was 132 ± 32 µmol TE/g dry weight SPL. SPL1 had the highest antioxidant potential, followed by SPL9, SPL3, SPL28, and SPL11 (P < 0.05). The concentrations of major identified phenolics from the five SPL samples (#1, 9, 3, 28, and 11) in dry weight of SPL were: chlorogenic acid at 3.05 ± 0.35 mg/g, 5-monocaffeoylquinic acid (CQA) at 0.71 ± 0.06 mg chlorogenic acid equivalent (ChAE)/g, 4-CQA at 0.99 ± 0.07 mg ChAE/g, 3,4-diCQA at 1.22 ± 0.18 mg ChAE/g, 3,4,5-triCQA at 1.2 ± 0.18 mg ChAE/g, and 3,5-diCQA at 15.5 ± 4.05 mg ChAE/g. 3,5-diCQA, the predominant phenolic, was present in the highest amounts in SPL1. Conclusions The results showed that sweetpotato leaves collected in 2019 contained higher total phenolics and antioxidant capacity than the ones collected in 2018. Phenolic compounds have strong antioxidant activity in Arkansas-grown sweetpotato leaves. This study warrants further investigation of sweetpotato leaves to be utilized as an antioxidant. Funding Sources The work was supported by USDA-NIFA.

scholarly journals Comparison of Phenolic Compounds and Antioxidant Activities of Raw and Cooked Turkish Artichoke Cultivars

2021 ◽  
Vol 5 ◽  
Author(s):  
Seda Kayahan ◽  
Didem Saloglu
Keyword(s):  
Phenolic Compounds ◽  
Chlorogenic Acid ◽  
Acid Content ◽  
Total Phenolic Content ◽  
Phenolic Content ◽  
Antioxidant Activities ◽  
Total Phenolic ◽  
Total Phenolic Compounds ◽  
Microwave Cooking ◽  
Gallic Acid Equivalent

The objective of this work was to determine the total phenolic compounds and antioxidants in raw and cooked Sakiz and Bayrampasa variety artichokes in parts such as inner bracts, stems, receptacles, and outer bracts. The artichokes were cooked by boiling, microwaving, and baking methods, and total phenolic compounds and antioxidants of cooked artichokes were evaluated. While TPC (total phenolic content), DPPH (2,2-diphenyl-1-picryl-hydrazine), and CUPRAC values for the leaves of raw Bayrampasa artichoke were found to be 686 mg gallic acid equivalent (GAE)/100 g, 478 mg TE/100 g, and 4,875 mg TE/100 g, respectively, TPC, DPPH, and CUPRAC values for stems of Sakiz artichoke were determined to be 1,579 mg GAE/100 g, 1,259 mg TE/100 g, and 3,575 mg TE/100 g. A significant increase in the content of TPC, DPPH, and CUPRAC values was observed for all cooking applications of both artichokes. DPPH and CUPRAC values increased by 11 and 43 times and 17 and 6.7 times after baking of Sakiz and microwave cooking of Bayrampasa, respectively. Chlorogenic acid, cynarine, and cynaroside content of both artichokes had an increment after all cooking applications. Chlorogenic acid content was improved 29 and 58 times after baking of Sakiz and microwave cooking of Bayrampasa, respectively.

scholarly journals Green Extraction of Six Phenolic Compounds from Rattan (Calamoideae faberii) with Deep Eutectic Solvent by Homogenate-Assisted Vacuum-Cavitation Method

Molecules ◽  
2018 ◽  
Vol 24 (1) ◽  
pp. 113 ◽  
Author(s):  
Qin Cao ◽  
Junhan Li ◽  
Yu Xia ◽  
Wei Li ◽  
Sha Luo ◽  
...  
Keyword(s):  
Phenolic Compounds ◽  
Chlorogenic Acid ◽  
Gallic Acid ◽  
Total Phenolic Content ◽  
Protocatechuic Acid ◽  
Phenolic Content ◽  
Deep Eutectic Solvent ◽  
Epigallocatechin Gallate ◽  
Total Phenolic ◽  
Protocatechuic Aldehyde

A homogenate-assisted vacuum-cavitation extraction (HVE) method with a “green” solvent (a deep eutectic solvent, DES) was developed to extract phenolic compounds from rattan (Calamoideae faberii). In this study, the optimum molar ratio of choline chloride (ChCl) and ethylene glycol (EG) was 1:3, the optimum volume ratio of ChCl-EG:H2O was 6:4, the solid-liquid ratio of HVE was 1:15, and the extraction time of homogenate and vacuum-cavitation were 2.0 min and 25 min, respectively. Under the optimum parameters of HVE, the extraction yield of total phenolic content with ChCl-EG solution was 6.82 mg/g. The higher total phenolic content was detected in fruit tissues (seeds 81.24 ± 1.55 mg/g, episperm 43.21 ± 0.87 mg/g, and arillus 38.47 ± 0.74 mg/g), followed by in leaves (sheath 19.5 ± 0.38 mg/g and blade 17.81 ± 0.33 mg/g). In addition, the content of specific phenolic compounds in aqueous and DES extracts was determined. Chlorogenic acid was the most abundant phenol in most organs of the rattan plant. Gallic acid was mainly distributed in the arillus; protocatechuic acid was mainly distributed in the arillus, sheath, and blade; protocatechuic aldehyde was mainly distributed in the blade, seed, and sheath; (+)-catechins were mainly distributed in the episperm, seed, and sheath; and epigallocatechin gallate was mainly distributed in the blade. The recovery rates of gallic acid, protocatechuic acid, protocatechuic aldehyde, (+)-catechins, chlorogenic acid, and epigallocatechin gallate were 93.77%, 94.09%, 97.32%, 97.83%, 94.41%, and 92.47%, respectively, by AB-8 resin.

Microwave Pretreatment of Vegetable Materials to Increase the Extraction Yield of Natural Products

2018 ◽  
Vol 69 (8) ◽  
pp. 1976-1979
Author(s):  
Ioana Asofiei ◽  
Ioan Calinescu ◽  
Adina Ionuta Gavrila ◽  
Daniel Ighigeanu ◽  
Diana Martin
Keyword(s):  
Natural Products ◽  
Chemical Composition ◽  
Phenolic Compounds ◽  
Total Phenolic Content ◽  
Phenolic Content ◽  
Extraction Yield ◽  
Total Phenolic ◽  
Microwave Pretreatment ◽  
Pretreatment Time ◽  
Conventional Extraction

It was designed and built a laboratory experimental installation (LEI) for the microwave pretreatment of vegetable materials. To study the influence of microwave pretreatment on the total phenolic content (TPC), a conventional extraction of polyphenols from treated and untreated fresh sea buckthorn leaves was performed. For short extraction times, the amount of phenolic compounds was higher for the extracts obtained from treated leaves, but a long pretreatment time (28 s) led to a decrease in TPC. The qualitative analysis showed that the chemical composition is not affected by the microwave pretreatment.

Color and Antioxidant Changes in Various Natural Rubber Processes

2015 ◽  
Vol 754-755 ◽  
pp. 230-234 ◽  
Author(s):  
Suwimon Siriwong ◽  
Adisai Rungvichaniwat ◽  
Pairote Klinpituksa ◽  
Khalid Hamid Musa ◽  
Aminah Abdullah
Keyword(s):  
Acetic Acid ◽  
Natural Rubber ◽  
Retention Index ◽  
Total Phenolic Content ◽  
Room Temperature ◽  
Phenolic Content ◽  
Total Phenolic ◽  
Lovibond Colorimeter

Fresh field natural rubber was coagulated by acetic acid, soaked in water at room temperature (WRT) or 70°C (W70) for 1 hr, and then dried in an oven at 40°C. Non-soaked natural rubber samples (NoW) served as a control. Two grades of natural rubber, namely air-dry sheet (ADS) and ribbed smoked sheet No.3 (RSS3) derived from the same latex, were also investigated. All dry rubber samples were characterized with Lovibond colorimeter according to ASTM D3157, as well as with a HunterLab spectrophotometer. Furthermore, all the dry rubber samples were dissolved in a chloroform:methanol mixture (4:1 v:v). The rubber was then precipitated out of the solution with methanol, and the remaining solution was quantitatively analyzed for total phenolic content (TPC). The plasticity retention index (PRI) was determined for all the dried rubber samples according to ASTM D3194. It was found that WRT, W70 and ADS were similar in lightness L*, while RSS3 had the lowest L*. W70 had the lowest redness a*, which increased in the order WRT, NoW, RSS3 and ADS. W70 also had the lowest yellowness b*, which increased in the order RSS3, NoW and WRT and ADS. Moreover, TPC was the lowest for the W70 sample, increasing in the order ADS, WRT, NoW and RSS3. The PRI was highest for W70, and decreased in the order WRT, RSS3, NoW and ADS. All of the PRI values observed were comparatively high relative to blocked standard Thai rubber 20 (STR20).

scholarly journals Interspecific variation in total phenolic content in temperate brown algae

2017 ◽  
Vol 90 (1) ◽  
Author(s):  
Anna Maria Mannino ◽  
Valentina Vaglica ◽  
Elisabetta Oddo
Keyword(s):  
Phenolic Compounds ◽  
Brown Algae ◽  
Total Phenolic Content ◽  
Phenolic Content ◽  
Interspecific Variation ◽  
Multiple Roles ◽  
Total Phenolic ◽  
Phenolic Contents ◽  
Cystoseira Compressa ◽  
Protection Mechanisms

Marine algae synthesize secondary metabolites such as polyphenols that function as defense and protection mechanisms. Among brown algae, Fucales and Dictyotales (Phaeophyceae) contain the highest levels of phenolic compounds, mainly phlorotannins, that play multiple roles. Four temperate brown algae (Cystoseira amentacea, Cystoseira compressa, Dictyopteris polypodioides and Padina pavonica) were studied for total phenolic contents. Total phenolic content was determined colorimetrically with the Folin-Ciocalteu reagent. Significant differences in total phenolic content were observed between leathery and sheetlike algae and also within each morphological group. Among the four species, the sheet-like alga D. polypodioides, living in the upper infralittoral zone, showed the highest concentration of phenolic compounds. These results are in agreement with the hypothesis that total phenolic content in temperate brown algae is influenced by a combination of several factors, such as growth form, depth, and exposition to solar radiation.

scholarly journals Effects of Different Combination of Growing Media on Growth and Total Phenolic Content in Labisia pumila (Kacip Fatimah)

2020 ◽  
Vol 6 (2) ◽  
pp. 258-261
Author(s):  
Md. Ariff Farah Fazwa ◽  
Saffie Norhayati ◽  
Samsul Bahari Syafiqah Nabilah ◽  
Sundara Rajoo Keeren
Keyword(s):  
Phenolic Compounds ◽  
Total Phenolic Content ◽  
Phenolic Content ◽  
Growth Parameters ◽  
Abiotic Factors ◽  
Total Phenolic ◽  
Growth Media ◽  
Growing Media ◽  
Significant Difference ◽  
Labisia Pumila

Labisia pumila, locally known in Malaysia as Kacip Fatimah, is highly-prized for its phenols. Phenolic compounds in L. pumila has numerous pharmacological properties such as having anti-oxidant, anti-microbial, anti-inflammatory and anti-cancer potential. The production of phenolic contents in plants is influence by various factor, including abiotic factors such as soil properties. Thus, the objective of this study was to determine the effects of different combinations of growing media on the growth and total phenolic content in L. pumila leaves. Six combinations of growing media were used, each using different types of compost that had differing amounts of L. pumila leaves. The plants were grown in all media for a period of six months at FRIM’s nursery. Besides growth performance, the total phenolic content was also determined, six months after planting. There was no significant difference in growth parameters for all treatments, with the exception of control (significantly lower than all treatments). However, the total phenolic content of Treatment 1 (80.6 mg GAE/g) was significantly higher than all other treatments. This can be attributed to the properties of Compost 1 (which was in the growth media of Treatment 1), as it had the highest pH (5.26), the highest potassium concentration (0.7%) and high nitrogen concentrations (1.32%). The composition of Compost 1 was 5 kg dry leaves + 500 g dry Kacip Fatimah + Slow Release Fertilizer (SRF). Results from this study showed that growing media is a key factor that leads to significant increment in phenolic compounds in L. pumila.

scholarly journals Effects of temperature, steeping time and particle size used in infusion brewing on total phenolic content and antioxidant activity of tea produced from young upland rice leaves

2021 ◽  
Vol 21 (02) ◽  
pp. 17477-17491
Author(s):  
Narissara Uthai ◽  
Keyword(s):  
Antioxidant Activity ◽  
Total Phenolic Content ◽  
Room Temperature ◽  
Phenolic Content ◽  
Antioxidant Activities ◽  
Upland Rice ◽  
Total Phenolic ◽  
Antioxidant Power ◽  
Surface Areas ◽  
Young Leaves

Tea was brewed from young leaves of 4 weeks old Thai upland black waxy rice plants of the cultivar ‘Damgatondam’, which has a noticeable fragrant smell from both seeds and leaves. The plants were harvested about 1 cm above soil level, washed, and cut into pieces, about 1 cm long. These pieces were then steamed for 1 min in a steamer rack, withering for 5min by spreading them out in a thin layer, pan-roasted at 70°C for 30 min, and finally dried at 70°C for 90 min. They were either kept as pieces of leaves or ground into a powder, and both were used for brewing tea by infusion in water at 4oC, room temperature (about 25oC), for 1, 3, 5, 7, 9, 11, or 15 h and brewing at 70oC or 98oC for 1, 3, 5, 7, 9, 11 or 15 min. Directly after brewing, the tea was stored at -25oC awaiting analysis. Each sample was analyzed for total phenolic content (TPC) and for antioxidant activity, by ferric reducing antioxidant power (FRAP), 2,2 diphenyl-1-picrylhydrazyl (DPPH) and 2,2'-azino-bis (3-ethylbenzothiazo line-6-sulphonic acid) (ABTS) assays. Results showed that tea brewed from ground leaves had higher antioxidant activity than tea brewed from ungrounded leaves. The highest level of extraction was at 98oC for 15 min, where the TPC was 62.79 mg GAE/100g, antioxidant activity was 278.04 for DPPH, 195.38 for ABTS and 1150.13 mg TE/100g for FRAP. Samples extracted at 4oC or room temperature also had high levels of TPC and antioxidant activity with the maximum extraction at room temperature for 15 h; where TPC was 46.76 mg GAE/100g, and antioxidant activities were 152.72 for DPPH, 140.42 for ABTS and 950.32 mg TE/100 g for FRAP. Therefore, the level of extraction for tea brewed in all four conditions depended on both temperature and brewing time. Also, extraction of high levels of bioactive compounds depended on large surface areas of the leaves being in contact with water.

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