The Dynamics of the Bacterial Community of the Photobioreactor-Cultivated Green Microalga Haematococcus lacustris during Stress-Induced Astaxanthin Accumulation

Haematococcus lacustris is a natural source of a valuable ketocarotenoid astaxanthin. Under autotrophic growth conditions, it exists in the form of a community with bacteria. The close coexistence of these microorganisms raises two questions: how broad their diversity is and how they interact with the microalga. Despite the importance these issues, little is known about microorganisms existing in Haematococcus cultures. For the first time, we characterize the dynamic of the H. lacustris microbiome of the microbiome of Haematococcus (a changeover of the bacterial associated species as function of the time) cultivated autotrophically in a photobioreactor based on 16S rRNA metabarcoding data. We found that Proteobacteria and Bacteroidetes are predominant phyla in the community. The Caulobacter bacterium became abundant during astaxanthin accumulation. These data were supported by microscopy. We discuss possible roles and interactions of the community members. These findings are of potential significance for biotechnology. They provide an insight into possible bacterial contamination in algal biomass and reveal the presence of bacteria essential for the algal growth.

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Aleksey Peshkov as an editor of Samarskaya Gazeta

Voprosy literatury ◽

10.31425/0042-8795-2020-4-128-138 ◽

2020 ◽

pp. 128-138

Author(s):

A. S. Bik-Bulatov

Keyword(s):

Local History ◽

Organizational Skills ◽

First Time ◽

New Evidence ◽

Insight Into ◽

Shed Light

The article uses little known letters of M. Gorky, many of which were published for the first time in 1997, as well as findings of Samara-based experts in local history to shed light on the writer’s work as editor-in-chief of the Samarskaya Gazeta newspaper in 1895. The researcher introduces hitherto unstudied reminiscences of the journalist D. Linyov (Dalin) about this period, which reference a letter by Gorky, now lost. The paper details a newly discovered episode of Gorky’s professional biography as a journalist: it concerns his campaign against a Samara ‘she-wolf,’ the madam of a local brothel A. Neucheva. Linyov’s reminiscences turn out to be an important and interesting source, offering an insight into the daily grind of the young editor Gorky, providing new evidence of his excellent organizational skills, and describing his moral and social stance. The author presents his work in the context of a recently initiated broader discussion about the need to map out all Russian periodicals for the period until 1917, as well as all research devoted to individual publications.

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The Clogging of Sewage Lagoon Aerators

Water Science & Technology ◽

10.2166/wst.1989.0269 ◽

1989 ◽

Vol 21 (6-7) ◽

pp. 669-676

Author(s):

E. Davis

Keyword(s):

Waste Water ◽

Water Treatment ◽

Waste Water Treatment ◽

Algal Growth ◽

Growth Conditions ◽

Field Investigation ◽

Large Bubble ◽

Physical Factors ◽

Pull System ◽

Fine Bubble

The Lagoon method of waste water treatment was introduced into Canada in the mid 1950's. At present, of the 2019 waste water treatment systems in Canada, 1163 are Lagoon systems and 265 of these include aeration equipment. Continuing difficulties associated with the plugging of the aerators have led to a study of this maintenance problem. Physical factors have been identified as the major cause of the plugging of large bubble aerators; whereas chemical solution and deposition under high algal growth conditions have been set forward as the causes of fine bubble aerator plugging. Selected field investigation data collected indicate that the proposed push-pull system of precipitation of calcium carbonate and silica dioxide may in fact be responsible for the plugging of fine bubble aerators with these materials.

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The participation of Palestinian-Israeli politicians in public and commercial television and radio in Israel as capability

International Communication Gazette ◽

10.1177/1748048521990565 ◽

2021 ◽

pp. 174804852199056

Author(s):

Baruch Shomron ◽

Amit Schejter

Keyword(s):

Capabilities Approach ◽

Media Representations ◽

Community Members ◽

Commercial Television ◽

Radio Stations ◽

Modes Of Representation ◽

Current Affairs ◽

Different Types ◽

The Media ◽

Insight Into

This study examines how media representations of Palestinian-Israeli politicians, can help community members realize their capabilities. The study’s database is comprised of 1,207 interviews conducted with Palestinian-Israeli politicians on news and current affairs programs on the three national television channels and the two national radio stations in Israel, for 24 months (2016-2017). We identified and analyzed the differences in the modes of representation between national and local Palestinian-Israeli politicians and between Palestinian-Israeli parliament members in the Joint List and Palestinian-Israeli parliament members in Zionist parties, all through the capabilities prism. In this study, we demonstrated how different types of Palestinian-Israeli politicians may potentially affect the realization of different political functions and capabilities. Analyzing political representations in the media through the theoretical framework of the ‘capabilities approach’ contributes to a more comprehensive insight into the roles the media can play promoting people’s wellbeing and human rights, relative to traditional media theories.

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Comparative Study of Chemical Compositions and Antioxidant Capacities of Oils Obtained from 15 Macadamia (Macadamia integrifolia) Cultivars in China

Foods ◽

10.3390/foods10051031 ◽

2021 ◽

Vol 10 (5) ◽

pp. 1031

Author(s):

Xixiang Shuai ◽

Taotao Dai ◽

Mingshun Chen ◽

Ruihong Liang ◽

Liqing Du ◽

...

Keyword(s):

Palmitoleic Acid ◽

Linear Regression Analysis ◽

Monounsaturated Fatty Acids ◽

Multiple Linear Regression Analysis ◽

Chemical Compositions ◽

Macadamia Integrifolia ◽

Antioxidant Capacities ◽

Squalene Content ◽

First Time ◽

Insight Into

The planting area of macadamia in China accounted for more than one third of the world’s planted area. The lipid compositions, minor components, and antioxidant capacities of fifteen varieties of macadamia oil (MO) in China were comparatively investigated. All varieties of MO were rich in monounsaturated fatty acids, mainly including oleic acid (61.74–66.47%) and palmitoleic acid (13.22–17.63%). The main triacylglycerols of MO were first time reported, including 19.2–26.1% of triolein, 16.4–18.2% of 1-palmitoyl-2,3-dioleoyl-glycerol, and 11.9–13.7% of 1-palmitoleoyl-2-oleoyl-3-stearoyl-glycerol, etc. The polyphenol, α-tocotrienol and squalene content varied among the cultivars, while Fuji (791) contained the highest polyphenols and squalene content. Multiple linear regression analysis indicated the polyphenols and squalene content positively correlated with the antioxidant capacity. This study can provide a crucial directive for the breeding of macadamia and offer an insight into industrial application of MO in China.

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ASKAP observations of multiple rapid scintillators reveal a degrees-long plasma filament

Monthly Notices of the Royal Astronomical Society ◽

10.1093/mnras/stab139 ◽

2021 ◽

Vol 502 (3) ◽

pp. 3294-3311

Author(s):

Yuanming Wang ◽

Artem Tuntsov ◽

Tara Murphy ◽

Emil Lenc ◽

Mark Walker ◽

...

Keyword(s):

Physical Properties ◽

Angular Width ◽

Scattering Medium ◽

Central Frequency ◽

Linear Arrangement ◽

Plasma Filament ◽

Kinematic Models ◽

Tidal Stream ◽

First Time ◽

Insight Into

ABSTRACT We present the results from an Australian Square Kilometre Array Pathfinder search for radio variables on timescales of hours. We conducted an untargeted search over a 30 deg2 field, with multiple 10-h observations separated by days to months, at a central frequency of 945 MHz. We discovered six rapid scintillators from 15-min model-subtracted images with sensitivity of $\sim\! 200\, \mu$Jy/beam; two of them are extreme intra-hour variables with modulation indices up to $\sim 40{{\ \rm per\ cent}}$ and timescales as short as tens of minutes. Five of the variables are in a linear arrangement on the sky with angular width ∼1 arcmin and length ∼2 degrees, revealing the existence of a huge plasma filament in front of them. We derived kinematic models of this plasma from the annual modulation of the scintillation rate of our sources, and we estimated its likely physical properties: a distance of ∼4 pc and length of ∼0.1 pc. The characteristics we observe for the scattering screen are incompatible with published suggestions for the origin of intra-hour variability leading us to propose a new picture in which the underlying phenomenon is a cold tidal stream. This is the first time that multiple scintillators have been detected behind the same plasma screen, giving direct insight into the geometry of the scattering medium responsible for enhanced scintillation.

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Rapid systemic surge of IL-33 after severe human trauma: a prospective observational study

Molecular Medicine ◽

10.1186/s10020-021-00288-1 ◽

2021 ◽

Vol 27 (1) ◽

Author(s):

Olav Sundnes ◽

William Ottestad ◽

Camilla Schjalm ◽

Peter Lundbäck ◽

Lars la Cour Poulsen ◽

...

Keyword(s):

High Resolution ◽

Prospective Observational Study ◽

Tissue Injury ◽

Trauma Patients ◽

Decoy Receptor ◽

Interleukin 33 ◽

Injured Patients ◽

First Time ◽

Kinetics Of ◽

Insight Into

Abstract Background Alarmins are considered proximal mediators of the immune response after tissue injury. Understanding their biology could pave the way for development of new therapeutic targets and biomarkers in human disease, including multiple trauma. In this study we explored high-resolution concentration kinetics of the alarmin interleukin-33 (IL-33) early after human trauma. Methods Plasma samples were serially collected from 136 trauma patients immediately after hospital admission, 2, 4, 6, and 8 h thereafter, and every morning in the ICU. Levels of IL-33 and its decoy receptor sST2 were measured by immunoassays. Results We observed a rapid and transient surge of IL-33 in a subset of critically injured patients. These patients had more widespread tissue injuries and a greater degree of early coagulopathy. IL-33 half-life (t1/2) was 1.4 h (95% CI 1.2–1.6). sST2 displayed a distinctly different pattern with low initial levels but massive increase at later time points. Conclusions We describe for the first time early high-resolution IL-33 concentration kinetics in individual patients after trauma and correlate systemic IL-33 release to clinical data. These findings provide insight into a potentially important axis of danger signaling in humans.

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Airborne Algal growth on roofs of membrane-structured residences in cold area of Japan

Journal of Building Physics ◽

10.1177/1744259120980034 ◽

2020 ◽

pp. 174425912098003

Author(s):

Makiko Nakajima ◽

Daisuke Masueda ◽

Shuichi Hokoi ◽

Takayuki Matsushita

Keyword(s):

Algal Growth ◽

Residential Building ◽

Growth Conditions ◽

Building Structure ◽

Transparent Film ◽

Cold Region ◽

Algal Population ◽

Building Facades ◽

Cold Area ◽

Survey Results

The discoloration of building facades due to airborne algae is observed in our surroundings. The growth conditions of these algae are not yet fully understood, and efficient measures for preventing the growth of the algae are not presently available. The objective of this study was to investigate the effects of the ambient environment and building structure on algal growth. A residential building in a cold region of Japan was surveyed. The roof was a multi-layered structure comprising a semi-transparent film, an air layer, and a layer of insulation from the outside, supported by rafters. The soiled state was visually observed by taking photographs. On the northeast (NE) and northwest (NW) roofs, several black stripes appeared 4 months after cleaning. The soiling increased in the spring and autumn. The soiling first appeared on the film backed by the rafter and then extended to the film backed by the air layer. The condensation time during the day in the rafter part was longer than that in the air-layer part. Condensation occurred during the night, but its frequency exhibited no dependence on the orientation of the roof. Algae tend to die when exposed to an environment with a temperature higher than 45°C. The NE roof had the shortest period with a surface temperature of >45°C. These measurements agreed well with the survey results, which indicated that the soiling mainly occurred on the NE and NW sides of the roofs. The time for algal growth was estimated under the assumption that algae can grow at surface temperatures ranging from 0 to 45°C, in agreement with the observed soiling. The observed soiling changes were well explained by the algal population calculated via a growth predictive model according to the algal temperature and relative humidity.

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Insight into the Secondary Metabolites of Geum urbanum L. and Geum rivale L. Seeds (Rosaceae)

Plants ◽

10.3390/plants10061219 ◽

2021 ◽

Vol 10 (6) ◽

pp. 1219

Author(s):

Marek Bunse ◽

Peter Lorenz ◽

Florian C. Stintzing ◽

Dietmar R. Kammerer

Keyword(s):

Fatty Acids ◽

Phenolic Compounds ◽

Unsaturated Fatty Acids ◽

Asiatic Acid ◽

Cosmetic Products ◽

Nutritional Properties ◽

First Time ◽

Derivatives Of ◽

Geum Urbanum ◽

Insight Into

The present study aimed at the identification and quantitation of phenolic compounds, fatty acids, and further characteristic substances in the seeds of Geum urbanum L. and Geum rivale L. For this purpose, individual components of extracts recovered with MeOH, CH2Cl2, and by cold-pressing, respectively, were characterized by HPLC-DAD/ESI-MSn and GC/MS and compared with reference compounds. For both Geum species, phenolic compounds, such as flavonoids and gallic acid derivatives, and triterpenes, such as saponins and their aglycones, were detected. Surprisingly, both Geum species revealed the presence of derivatives of the triterpenoid aglycons asiatic acid and madecassic acid, which were characterized for the first time in the genus Geum. Furthermore, the fatty acids of both species were characterized by GC–MS after derivatization. Both species showed a promising fatty-acid profile in terms of nutritional properties because of high proportions of unsaturated fatty acids. Linoleic acid and linolenic acid were most abundant, among other compounds such as palmitic acid and stearic acid. In summary, the present study demonstrates the seeds of G. urbanum and G. rivale to be a valuable source of unsaturated fatty acids and bioactive phenolics, which might be exploited for nutritional and cosmetic products and for phytotherapeutic purposes.

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The carmine dilemma: does the natural colourant preference outweigh nausea?

British Food Journal ◽

10.1108/bfj-12-2017-0671 ◽

2018 ◽

Vol 120 (8) ◽

pp. 1915-1928

Author(s):

Judith Müller-Maatsch ◽

Johannes Jasny ◽

Katharina Henn ◽

Claudia Gras ◽

Reinhold Carle

Keyword(s):

Design Methodology ◽

Consumer Perception ◽

Natural Food ◽

German Population ◽

Misleading Information ◽

Content Type ◽

Broad Cross Section ◽

First Time ◽

Self Administered Questionnaire ◽

Insight Into

Purpose The purpose of this paper is to provide insight into the consumers’ perception of natural and artificial food colourants. Furthermore, attitudes towards the application of carmine, being technically important and ubiquitously used to impart red shades, are assessed and analysed. Originating from insects, carmine is considered as natural but may arouse disgust. Design/methodology/approach In total, 625 individuals were surveyed using an online, self-administered questionnaire to represent a broad cross-section of the German population. Findings Independent of their origin, the application of colourants was rejected by 57.0 per cent of the interviewees. In total, 31.8 per cent of the participants stated a neutral attitude, while only 11.2 per cent expressed a positive notion. Most respondents preferred colourants from natural sources to artificial ones. While consumers perceive natural food colourants composed of genuine plant pigments positively, 61.6 per cent of respondents disliked the application of animal-derived colourants, 24.8 per cent of them did neither reject nor like it, and only 13.6 per cent of the interviewees stated a positive attitude towards them. The findings of this paper further indicate consumers’ preference for colourants to be either artificial or plant-derived rather than carmine. Food colourants are being rejected, possibly due to misleading information and confusing labelling. Consequently, information about carmine, including its origin and production, did not increase the aversion to products that are dyed with it, but increased their acceptance. Originality/value This study outlines consumer perception and attitudes towards food colourants. For the first time, the findings of this paper report the effect of revealing information about an additive, which initially aroused disgust, and its influence on consumer perception.

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Direct measurement of Coulomb-laser coupling

Scientific Reports ◽

10.1038/s41598-020-79805-x ◽

2021 ◽

Vol 11 (1) ◽

Author(s):

Doron Azoury ◽

Michael Krüger ◽

Barry D. Bruner ◽

Olga Smirnova ◽

Nirit Dudovich

Keyword(s):

Coulomb Interaction ◽

Time Domain ◽

Coulomb Potential ◽

Laser Field ◽

Large Range ◽

Extreme Ultraviolet ◽

Strong Field ◽

The Time Domain ◽

First Time ◽

Insight Into

AbstractThe Coulomb interaction between a photoelectron and its parent ion plays an important role in a large range of light-matter interactions. In this paper we obtain a direct insight into the Coulomb interaction and resolve, for the first time, the phase accumulated by the laser-driven electron as it interacts with the Coulomb potential. Applying extreme-ultraviolet interferometry enables us to resolve this phase with attosecond precision over a large energy range. Our findings identify a strong laser-Coulomb coupling, going beyond the standard recollision picture within the strong-field framework. Transformation of the results to the time domain reveals Coulomb-induced delays of the electrons along their trajectories, which vary by tens of attoseconds with the laser field intensity.

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