scholarly journals Effects of Pulsed Electric Field on the Cell Wall and Infection Activity of Rhizoctonia solani

Biology ◽  
2019 ◽  
Vol 8 (4) ◽  
pp. 73
Author(s):  
Feng ◽  
He ◽  
Pan ◽  
Xu ◽  
Xue ◽  
...  
Keyword(s):  
Cell Wall ◽  
Electric Field ◽  
Rhizoctonia Solani ◽  
Pulse Width ◽  
Processing Time ◽  
Pulsed Electric Field ◽  
Pulse Voltage ◽  
Control Group ◽  
Voltage Amplitude ◽  
Polygalacturonase Activity

This paper adopts the Design-Expert software to design an orthogonal experiment with a pulse voltage amplitude of 30 kV, processing time of three minutes, and a pulse width of 45 μs as the center points, in order to study the effects of the pulsed electric field on the cell wall and infection activity of Rhizoctonia solani. High-voltage pulse power was used to treat the bacteria solution with the pulsed electric field. Untreated Rhizoctonia solani were used as the control group. Transmission electron microscope images were used to analyze the cell wall damage. ANOVA was performed on the experimental results and the fitting degree of the model was good (F>>1). Response surface analysis was used to optimize the parameters based on chitin content and polygalacturonase activity. The optimal treatment conditions were obtained as a pulse voltage amplitude of 25 kV, processing time of 2.54 min, and a pulse width of 34.35 μs. On this basis, experiments were designed to verify the optimized conditions. The results demonstrated that, under the optimal processing conditions, the damage index of the cell wall of Rhizoctonia solani was 9.59% lower in chitin content and 83.05% lower in polygalacturonase activity compared with those of the control group. All indexes were significantly different (P < 0.001), which is consistent with the parameter optimization results. The results provide a theoretical basis for the pulsed electric field assisted sterilization and reference for the design of plant protection machinery in the latter stage.

scholarly journals Damaging Effects of Pulsed Electric Field Process Parameters on Rhizoctonia solani Cells Using Response Surface Methodology

2019 ◽  
Vol 9 (21) ◽  
pp. 4706
Author(s):  
Feng ◽  
Jiang ◽  
Zhu ◽  
Jiang ◽  
Yin ◽  
...  
Keyword(s):  
Cell Wall ◽  
Cell Membrane ◽  
Electric Field ◽  
Pulse Duration ◽  
Process Parameters ◽  
Processing Time ◽  
Membrane Damage ◽  
Voltage Amplitude ◽  
Cell Membrane Damage ◽  
Damage Rate

This work aimed to analyse the damaging effects of pulsed electric fields on Rhizoctonia solani. Design Expert software was used to design an orthogonal experiment. The cell membrane damage and cell wall damage were observed by scanning electron microscopy and quantitatively determined while using a conductivity metre and an ultraviolet spectrophotometer. The results showed that the cell membrane damage rate was correlated with the voltage amplitude and processing time (p < 0.01), while the effect of pulse duration was not significant (p > 0.05). Besides, the cell wall damage was related to electric field strength (voltage amplitude) (p < 0.01), while the pulse duration and processing time had no significant effect on that (p > 0.05). The optimal process parameters for this method were 25 kV/cm, 5 min., and a pulse duration of 60 µs. The optimised conditions were tested based on these results. When compared with Control Check (CK), the cell membrane damage rate was 48.72%, which was significantly higher than CK (p < 0.01).

Influence of Pulse Width on Polyphenol Extraction from Agricultural Products by Pulsed Electric Field

2013 ◽  
Vol 133 (2) ◽  
pp. 32-37 ◽  
Author(s):  
Akira Nakagawa ◽  
Hitoshi Hatayama ◽  
Koichi Takaki ◽  
Shoji Koide ◽  
Yukio Kawamura
Keyword(s):  
Electric Field ◽  
Pulse Width ◽  
Pulsed Electric Field ◽  
Agricultural Products ◽  
Polyphenol Extraction

scholarly journals Mild and Selective Protein Release of Cell Wall Deficient Microalgae with Pulsed Electric Field

2017 ◽  
Vol 5 (7) ◽  
pp. 6046-6053 ◽  
Author(s):  
Gerard P. ‘t Lam ◽  
Jelmer A. van der Kolk ◽  
Akshita Chordia ◽  
Marian H. Vermuë ◽  
Giuseppe Olivieri ◽  
...  
Keyword(s):  
Cell Wall ◽  
Electric Field ◽  
Pulsed Electric Field ◽  
Protein Release

scholarly journals Simulation of BNNSs Dielectrophoretic Motion under a Nanosecond Pulsed Electric Field

Nanomaterials ◽  
2021 ◽  
Vol 11 (3) ◽  
pp. 682
Author(s):  
Yan Mi ◽  
Xin Ge ◽  
Jinyan Dai ◽  
Yong Chen ◽  
Yakui Zhu
Keyword(s):  
Electric Field ◽  
Orientation Angle ◽  
Pulsed Electric Field ◽  
Basic Mechanism ◽  
Pulse Voltage ◽  
Movement Direction ◽  
Ultrapure Water ◽  
Nanosecond Pulsed Electric Field ◽  
Relaxation Polarization ◽  
High Level

Using a nanosecond pulsed electric field to induce orientation and arrangement of insulating flake particles is a novel efficient strategy, but the specific mechanism remains unclear. In this study, the dielectrophoretic motion of boron nitride nanosheets (BNNSs) in ultrapure water under a nanosecond pulsed electric field is simulated for the first time. First, the simulation theory is proposed. When the relaxation polarization time of the dielectric is much shorter than the pulse voltage width, the pulse voltage high level can be considered a short-term DC voltage. On this basis, the Arbitrary Lagrangian–Euler (ALE) method is used in the model, considering the mutual ultrapure water–BNNS particles-nanosecond pulsed electric field dielectrophoretic interaction, to study the influence of different BNNSs self-angle α and relative angle β on local orientation and global arrangement. The particles are moved by the dielectrophoretic force during the pulse voltage high level and move with the ultrapure water flow at the zero level, without their movement direction changing during this period, so the orientation angle and distance changes show step-like and wave-like curves, respectively. The model explains the basic mechanism of dielectrophoretic motion of BNNSs under a pulsed electric field and summarizes the motion law of BNNSs, providing a reference for subsequent research.

scholarly journals Pulsed electric field reduces the permeability of potato cell wall

2008 ◽  
Vol 29 (4) ◽  
pp. 296-301 ◽  
Author(s):  
Federico Gómez Galindo ◽  
P. Thomas Vernier ◽  
Petr Dejmek ◽  
António Vicente ◽  
Martin A. Gundersen
Keyword(s):  
Cell Wall ◽  
Electric Field ◽  
Pulsed Electric Field ◽  
Potato Cell

Effect of Pulsed Electric Field on Freeze-Drying of Potato Tissue

2014 ◽  
Vol 10 (4) ◽  
pp. 857-862 ◽  
Author(s):  
Yali Wu ◽  
Dongguang Zhang
Keyword(s):  
Energy Consumption ◽  
Field Strength ◽  
Electric Field ◽  
Electric Field Strength ◽  
Pulse Width ◽  
Unit Area ◽  
Pulsed Electric Field ◽  
Pulse Number ◽  
Drying Rate ◽  
Drying Time

Abstract The influence of pulsed electric field (PEF) on the drying behavior of potato was investigated, and the optimal parameters were determined. Drying experiments were conducted with different PEF pre-treatments. The effects of process parameters of PEF pre-treatment were examined with respect to drying rate, drying time, productivity per unit area, and energy consumption. Results showed that the three parameters investigated were significant in the following sequence: pulse number, electric field strength, and pulse width. The optimal electric field strength, pulse width, and pulse number were 1,500 V cm−1, 120 μs, and 45 pulses, respectively. Under these optimal conditions, productivity per unit area increased by 32.28%, specific energy consumption decreased by 16.59%, drying time was shortened by 31.47%, and drying rate improved by 14.31% compared with the control group.

Inactivation of Phytoplankton in Ship’s Ballast Water by Pulsed Electric Field and Thermal Treatment

2015 ◽  
Vol 1092-1093 ◽  
pp. 1060-1063
Author(s):  
Jun Feng Zhou ◽  
Ting Fei Xie ◽  
Le Ping Xu ◽  
Rui Zhao
Keyword(s):  
Thermal Treatment ◽  
Electric Field ◽  
Ballast Water ◽  
Pulse Width ◽  
Pulsed Electric Field ◽  
Aquatic Species ◽  
Electrode Gap ◽  
Preheating Temperature ◽  
Ship’S Ballast Water ◽  
Ship Ballast Water

Invasive aquatic species discharged through ship ballast water is one of the most serious problems posed nowadays in the marine environment. The effect of inactivation of phytoplankton by pulsed electric field and thermal treatment was studied. Effect factors such as pulsed voltage, electrode gap, pulse width and preheating temperature were explored, and its mechanism of inactivation was analyzed. The combined effect of mild thermal and PEF treatments was shown to give more effective damage of phytoplankton than application of PEF treatment alone. The untreated ballast water sample pretreated at moderate temperature (323 K) and moderate PEF (E ≈10kV/cm) showed a noticeable enhancing of inactivation.

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