scholarly journals Wound Healing Effects of Aloe muth-muth: In Vitro Investigations Using Immortalized Human Keratinocytes (HaCaT)

Biology ◽  
2020 ◽  
Vol 9 (11) ◽  
pp. 350
Author(s):  
Morné Fouché ◽  
Clarissa Willers ◽  
Sias Hamman ◽  
Christiaan Malherbe ◽  
Jan Steenekamp
Keyword(s):  
Wound Healing ◽  
Hacat Cell ◽  
Aloe Vera ◽  
Human Keratinocytes ◽  
Assay Method ◽  
Traditional Use ◽  
Cell Line Cell ◽  
Aloe Ferox ◽  
Cultivated Species

The traditional use of Aloe spp. for the purpose of wound healing has a long history and is widespread internationally. Recently, a hybrid aloe plant (Aloe muth-muth) has been cultivated by cross pollination between Aloe vera and Aloe ferox. The Aloe muth-muth plant has not yet been investigated for medicinal properties and provides an opportunity for potential biological activity, including wound healing. The aim of this study was to investigate the in vitro wound healing effects of both Aloe muth-muth gel and whole leaf material with the use of the immortalized human keratinocyte (HaCaT) cell line. Cell viability was conducted using methyl thiazolyl tetrazolium (MTT) assays. In vitro wound healing was tested on HaCaT cells using an established scratch assay method. The effect of Aloe muth-muth gel material on HaCaT cell migration was also investigated. Aloe muth-muth gel material exhibited statistically significantly (p < 0.05) higher percentage wound closure compared to the control at all three concentrations investigated. These findings confirm that this newly cultivated species, Aloe muth-muth, also possesses wound healing activity corresponding to that reported for the two species it is derived from, namely, Aloe vera and Aloe ferox. Therefore, Aloe muth-muth has the potential to be used in future wound therapeutics.

scholarly journals Achyrocline satureioides (Lam.) DC (Asteraceae) Extract-Loaded Nanoemulsions as a Promising Topical Wound Healing Delivery System: In Vitro Assessments in Human Keratinocytes (HaCaT) and HET-CAM Irritant Potential

Pharmaceutics ◽  
2021 ◽  
Vol 13 (8) ◽  
pp. 1241
Author(s):  
Lucélia Albarello Balestrin ◽  
Tainá Kreutz ◽  
Flávia Nathiely Silveira Fachel ◽  
Juliana Bidone ◽  
Nicolly Espindola Gelsleichter ◽  
...  
Keyword(s):  
Wound Healing ◽  
Hacat Cell ◽  
Chorioallantoic Membrane ◽  
Human Keratinocytes ◽  
Scratch Assay ◽  
Hen’S Egg ◽  
And Migration ◽  
Achyrocline Satureioides ◽  
Proliferation And Migration

Achyrocline satureioides (Lam.) DC Asteraceae extracts (ASEs) have been investigated for the treatment of various skin disorders. This study reports the effects of ASE-loaded nanoemulsions (NEASE) on the cellular viability, death by necrosis, and migration of immortalized human keratinocytes (HaCaT cell line), as well as the irritant potential through the hen’s egg chorioallantoic membrane test (HET-CAM). NEASE exhibited a polydispersity index above 0.12, with a droplet size of 300 nm, ζ-potential of −40 mV, and content of flavonoids close to 1 mg/mL. No cytotoxicity of the ASE was observed on HaCaT by MTT assay (up to 10 µg/mL). A significant increase of HaCaT viability was observed to NEASE (up to 5 μg/mL of flavonoids), compared to treatment with the ASE. The necrosis death evaluation demonstrated that only NEASE did not lead to cell death at all the tested concentrations. The scratch assay demonstrated that NEASE was able to increase the cell migration at low flavonoid concentrations. Finally, the HET-CAM test proved the non-irritative potential of NEASE. Overall, the results indicate the potential of the proposed formulations for topical use in wound healing, in view of their promising effects on proliferation and migration in keratinocytes, combined with an indication of the absence of cytotoxicity and non-irritating potential.

scholarly journals Human Keratinocyte Growth and Differentiation on Acellular Porcine Dermal Matrix in relation to Wound Healing Potential

2012 ◽  
Vol 2012 ◽  
pp. 1-8 ◽  
Author(s):  
Robert Zajicek ◽  
Vaclav Mandys ◽  
Ondrej Mestak ◽  
Jan Sevcik ◽  
Radana Königova ◽  
...  
Keyword(s):  
Wound Healing ◽  
Human Keratinocytes ◽  
Dermal Matrix ◽  
Keratinocyte Proliferation ◽  
Proliferation And Differentiation ◽  
A Cell ◽  
Cell Culture Assay ◽  
Air Liquid Interface

A number of implantable biomaterials derived from animal tissues are now used in modern surgery. Xe-Derma is a dry, sterile, acellular porcine dermis. It has a remarkable healing effect on burns and other wounds. Our hypothesis was that the natural biological structure of Xe-Derma plays an important role in keratinocyte proliferation and formation of epidermal architecturein vitroas well asin vivo. The bioactivity of Xe-Derma was studied by a cell culture assay. We analyzed growth and differentiation of human keratinocytes culturedin vitroon Xe-Derma, and we compared the results with formation of neoepidermis in the deep dermal wounds treated with Xe-Derma. Keratinocytes cultured on Xe-Derma submerged in the culture medium achieved confluence in 7–10 days. After lifting the cultures to the air-liquid interface, the keratinocytes were stratified and differentiated within one week, forming an epidermis with basal, spinous, granular, and stratum corneum layers. Immunohistochemical detection of high-molecular weight cytokeratins (HMW CKs), CD29, p63, and involucrin confirmed the similarity of organization and differentiation of the cultured epidermal cells to the normal epidermis. The results suggest that the firm natural structure of Xe-Derma stimulates proliferation and differentiation of human primary keratinocytes and by this way improves wound healing.

scholarly journals In Vitro Evaluation of Antioxidant, Anti-Inflammatory, Antimicrobial and Wound Healing Potential of Thymus Sipyleus Boiss. Subsp. Rosulans (Borbas) Jalas

Molecules ◽  
2019 ◽  
Vol 24 (18) ◽  
pp. 3353 ◽  
Author(s):  
Oya Ustuner ◽  
Ceren Anlas ◽  
Tulay Bakirel ◽  
Fulya Ustun-Alkan ◽  
Belgi Diren Sigirci ◽  
...  
Keyword(s):  
Wound Healing ◽  
Healing Process ◽  
Folk Medicine ◽  
Traditional Use ◽  
Necrosis Factor Alpha ◽  
Treatment Protocols ◽  
Fibroblast Migration ◽  
Anti Inflammatory ◽  
Treatment Of Wounds

Thymus sipyleus Boiss. subsp. rosulans (Borbas) Jalas (TS) is a commonly used plant in the treatment of various complaints, including skin wounds in Turkish folk medicine. Despite the widespread traditional use of TS, there is not any scientific report confirming the effectiveness of this plant on the healing process. This research aimed to investigate the effects of different extracts obtained from TS on biological events during wound healing, on a cellular basis. In this context, proliferative activities of the extracts, as well as the effects on wound closure and hydroxyproline synthesis, were determined. In addition to wound healing properties, the antioxidant, antibacterial and anti-inflammatory activities of the extracts were evaluated. Decoction (D) and infusion (I) extracts contained the highest amount of phenolic content and showed the most potent activity against DPPH radical. All extracts exhibited complete protection against the damage induced by hydrogen peroxide (H2O2) by increasing cell viability compared to only H2O2-treated groups, both in co-treatment and pre-treatment protocols. None of the extracts exhibited cytotoxic activity, and most of the extracts from the TS stimulated fibroblast proliferation and migration. All TS extracts exert anti-inflammatory activity by suppressing the overproduction of tumor necrosis factor-alpha (TNF-α) and nitric oxide (NO). The most pronounced activity on hydroxyproline synthesis was observed in D extract. In summary, it was observed that TS extracts can promote the healing process by enhancing fibroblast migration, proliferation and collagen synthesis as well as suppressing pro-inflammatory cytokines. The obtained data in this work support the traditional use of TS as a valuable plant-based compound for the treatment of wounds.

Aloesin from Aloe vera accelerates skin wound healing by modulating MAPK/Rho and Smad signaling pathways in vitro and in vivo

Phytomedicine ◽  
2017 ◽  
Vol 28 ◽  
pp. 19-26 ◽  
Author(s):  
Hussain Mustatab Wahedi ◽  
Minsun Jeong ◽  
Jae Kyoung Chae ◽  
Seon Gil Do ◽  
Hyeokjun Yoon ◽  
...  
Keyword(s):  
Wound Healing ◽  
Signaling Pathways ◽  
Aloe Vera ◽  
Skin Wound ◽  
Skin Wound Healing ◽  
Smad Signaling

scholarly journals Exploring theUrtica dioicaLeaves Hemostatic and Wound-Healing Potential

2017 ◽  
Vol 2017 ◽  
pp. 1-10 ◽  
Author(s):  
Karama Zouari Bouassida ◽  
Sana Bardaa ◽  
Meriem Khimiri ◽  
Tarek Rebaii ◽  
Slim Tounsi ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Wound Healing ◽  
Unsaturated Fatty Acids ◽  
Antioxidant Activities ◽  
High Concentration ◽  
Leaf Extracts ◽  
Traditional Use ◽  
Wound Healing Effect ◽  
Epidermal Regeneration

The present paper investigated the efficiency ofUrtica dioica (U. dioica)on hemostatic and wound healing activities.U. dioicaleaf extracts were evaluated for their antibacterial and antioxidant effects as well as their flavonoid and polyphenol content. The hydroethanolic extract (EtOH-H2OE), showing the most potent antibacterial and antioxidant activitiesin vitro, thanks to its flavonoid and polyphenol richness, was selected for hemostatic and wound healing evaluation. Twenty-four rats completing full-thickness wounds were split into four groups. The wounds were topically treated with saline solution, glycerol, “CICAFLORA,” andU. dioicaEtOH-H2OE (50 µL/mm2) until day 11. The wound healing effect was assessed by macroscopic, histological, and biochemical parameters. Rats treated with EtOH-H2OE showed fast wound closure (92.39%) compared to the control animals (60.91%) on the 11th day of wounding (P<0.01). Histopathological and biochemical explorations showed full epidermal regeneration and an improvement of the hydroxyproline content in theU. dioicaEtOH-H2OE treated rats. Analysis of fatty acids and sterols by GC-MS showed the presence of unsaturated fatty acids and a high concentration of lupeol known for their involvement in reepithelialization. These results prove the efficiency ofU. dioicaEtOH-H2OE in wound healing and supported its traditional use.

scholarly journals Antibacterial Multi-Layered Nanocellulose-Based Patches Loaded with Dexpanthenol for Wound Healing Applications

Nanomaterials ◽  
2020 ◽  
Vol 10 (12) ◽  
pp. 2469
Author(s):  
Daniela F. S. Fonseca ◽  
João P. F. Carvalho ◽  
Verónica Bastos ◽  
Helena Oliveira ◽  
Catarina Moreirinha ◽  
...  
Keyword(s):  
Wound Healing ◽  
Mechanical Performance ◽  
In Vitro Release ◽  
Human Keratinocytes ◽  
Controlled Drug Release ◽  
Release Mechanism ◽  
Layer By Layer ◽  
Number Of Layers ◽  
Migration Capacity

Antibacterial multi-layered patches composed of an oxidized bacterial cellulose (OBC) membrane loaded with dexpanthenol (DEX) and coated with several chitosan (CH) and alginate (ALG) layers were fabricated by spin-assisted layer-by-layer (LbL) assembly. Four patches with a distinct number of layers (5, 11, 17, and 21) were prepared. These nanostructured multi-layered patches reveal a thermal stability up to 200 °C, high mechanical performance (Young’s modulus ≥ 4 GPa), and good moisture-uptake capacity (240–250%). Moreover, they inhibited the growth of the skin pathogen Staphylococcus aureus (3.2–log CFU mL−1 reduction) and were non-cytotoxic to human keratinocytes (HaCaT cells). The in vitro release profile of DEX was prolonged with the increasing number of layers, and the time-dependent data imply a diffusion/swelling-controlled drug release mechanism. In addition, the in vitro wound healing assay demonstrated a good cell migration capacity, headed to a complete gap closure after 24 h. These results certify the potential of these multi-layered polysaccharides-based patches toward their application in wound healing.

Towards a modern approach to traditional use: in vitro and in vivo evaluation of Alchemilla vulgaris L. gel wound healing potential

2019 ◽  
Vol 238 ◽  
pp. 111789 ◽  
Author(s):  
Marija Tasić-Kostov ◽  
Ivana Arsić ◽  
Dragana Pavlović ◽  
Sanja Stojanović ◽  
Stevo Najman ◽  
...  
Keyword(s):  
Wound Healing ◽  
In Vivo Evaluation ◽  
Traditional Use ◽  
Modern Approach

scholarly journals In Vitro Gingival Wound Healing Activity of Extracts from Reynoutria japonica Houtt Rhizomes

Pharmaceutics ◽  
2021 ◽  
Vol 13 (11) ◽  
pp. 1764
Author(s):  
Izabela Nawrot-Hadzik ◽  
Adam Matkowski ◽  
Artur Pitułaj ◽  
Barbara Sterczała ◽  
Cyprian Olchowy ◽  
...  
Keyword(s):  
Wound Healing ◽  
Laser Scanning ◽  
Immunocytochemical Staining ◽  
Confocal Laser ◽  
Phytochemical Analysis ◽  
Chinese Medicinal Herb ◽  
Traditional Use ◽  
Reynoutria Japonica ◽  
Collagen Iii

Rhizomes of Reynoutria japonica Houtt. are a traditional Chinese medicinal herb (Polygoni cuspidati rhizoma, hu zhang) used for treatment of numerous diseases including wound healing support. The aim of this study was to provide evidence for the value of this herbal drug’s traditional use as a gingival healing treatment as well as to obtain the most active extract. In vitro studies were performed using primary human gingival fibroblasts (HGFs) with determination of viability (MTT assay), cell proliferation (the confocal laser scanning microscope (CLSM) was used to visualize histone 3 expression), cell migration (wound healing assay), and evaluation of the expression of collagen type III (immunocytochemical staining) after incubation with extracts from R. japonica rhizomes (25% or 40% ethanol or 60% acetone). In addition to these extracts, commercial dental rinse (containing chlorhexidine digluconate 0.2%) was tested as the gold standard of choice for gum healing in dental practice. The studied extracts were qualitatively and quantitatively characterized using the validated HPLC/DAD/ESI-HR-QTOF-MS method. Total phenols and tannins content were determined using the Folin–Ciocalteu assay. Low concentration of all extracts after 24 h incubation caused significant increase in HGF viability. This effect was most pronounced at a concentration of 50 µg/mL, which was selected for further experiments. All extracts (at 50 µg/mL) stimulated HGF to proliferate, migrate, and increase collagen III synthesis, but with different strength. The highest stimulated proliferation and migration activity was observed after incubation with 25% EtOH, which according to phytochemical analysis may be related to the highest content of resveratrol and an appropriate composition of procyanidins. The 25% EtOH extract from R. japonica rhizomes appears to be a promising gingival wound healing agent worthy of animal and clinical trials.

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