scholarly journals Facile Green, Room-Temperature Synthesis of Gold Nanoparticles Using Combretum erythrophyllum Leaf Extract: Antibacterial and Cell Viability Studies against Normal and Cancerous Cells

Antibiotics ◽  
2021 ◽  
Vol 10 (8) ◽  
pp. 893
Author(s):  
Olufunto T. Fanoro ◽  
Sundararajan Parani ◽  
Rodney Maluleke ◽  
Thabang C. Lebepe ◽  
Jose R. Varghese ◽  
...  
Keyword(s):  
Gold Nanoparticles ◽  
Cell Viability ◽  
Leaf Extract ◽  
Room Temperature ◽  
Klebsiella Oxytoca ◽  
Cost Effective ◽  
Average Diameter ◽  
Antibacterial Activities ◽  
Inhibition Concentration ◽  
First Time

We herein report a facile, green, cost-effective, plant-mediated synthesis of gold nanoparticles (AuNPs) for the first time using Combretum erythrophyllum (CE) plant leaves. The synthesis was conducted at room temperature using CE leaf extract serving as a reducing and capping agent. The as-synthesized AuNPs were found to be crystalline, well dispersed, and spherical in shape with an average diameter of 13.20 nm and an excellent stability of over 60 days. The AuNPs showed broad-spectrum antibacterial activities against both pathogenic Gram-positive (Staphylococcus epidermidis (ATCC14990), Staphylococcus aureus (ATCC 25923), Mycobacterium smegmatis (MC 215)) and Gram-negative bacteria (Proteus mirabilis (ATCC 7002), Escherichia coli (ATCC 25922), Klebsiella pneumoniae (ATCC 13822), Klebsiella oxytoca (ATCC 8724)), with a minimum inhibition concentration of 62.5 µg/mL. In addition, the as-synthesized AuNPs were highly stable with exceptional cell viability towards normal cells (BHK- 21) and cancerous cancer cell lines (cervical and lung cancer).

scholarly journals Ecofriendly Synthesis of Silver Nanoparticles Using Ethno Medicinal Plant Leaf Extract (Jasminum auriculatum) and their Antibacterial Properties

2015 ◽  
Vol 58 ◽  
pp. 113-121 ◽  
Author(s):  
S. Balasubramanian ◽  
U. Jeyapaul ◽  
S. Mary Jelastin Kala
Keyword(s):  
Silver Nanoparticles ◽  
Leaf Extract ◽  
Antibacterial Properties ◽  
Cost Effective ◽  
Average Diameter ◽  
Xrd Analysis ◽  
Antibacterial Activities ◽  
Aqueous Leaf Extract ◽  
Plant Leaf Extract

The present investigation, we describe a cost effective biofabrication of silver nanoparticles using jasminum auriculatum leaf extract. The aqueous leaf extract of jasminum auriculatum are used as reducing agent and capping agent in silver nanoparticles synthesis. The biofabricated silver nanoparticles were characterized by UV-Visible spectrophotometer, X-Ray Diffraction, FTIR spectra and SEM with EDX analysis. FTIR studies revealed that the functional group responsible for silver reduction were found to be jasminol and triterpinoid compounds present in leaf extract. XRD analysis showed that prepared silver nanoparticles are crystalline in nature with an average diameter of 15-20 nm. The prepared silver nanoparticles have been evaluated in vitro for antibacterial activities and found to have higher antibacterial activities.

scholarly journals Green Biosynthesis of Silver Nanoparticles Using Leaf Extract of Carissa carandas L. and Their Antioxidant and Antimicrobial Activity against Human Pathogenic Bacteria

Biomolecules ◽  
2021 ◽  
Vol 11 (2) ◽  
pp. 299
Author(s):  
Reetika Singh ◽  
Christophe Hano ◽  
Gopal Nath ◽  
Bechan Sharma
Keyword(s):  
Silver Nanoparticles ◽  
Pathogenic Bacteria ◽  
Leaf Extract ◽  
Room Temperature ◽  
Xrd Analysis ◽  
Antibacterial Activities ◽  
Effect Of Temperature ◽  
Human Pathogenic Bacteria ◽  
Carissa Carandas ◽  
Antioxidant And Antimicrobial Activity

Carissa carandas L. is traditionally used as antibacterial medicine and accumulates many antioxidant phytochemicals. Here, we expand this traditional usage with the green biosynthesis of silver nanoparticles (AgNPs) achieved using a Carissa carandas L. leaf extract as a reducing and capping agent. The green synthesis of AgNPs reaction was carried out using 1mM silver nitrate and leaf extract. The effect of temperature on the synthesis of AgNPs was examined using room temperature (25 °C) and 60 °C. The silver nanoparticles were formed in one hour by stirring at room temperature. In this case, a yellowish brown colour was developed. The successful formation of silver nanoparticles was confirmed by UV–Vis, Fourier transform infrared (FT-IR) and X-ray diffraction (XRD) analysis. The characteristic peaks of the UV-vis spectrum and XRD confirmed the synthesis of AgNPs. The biosynthesised AgNPs showed potential antioxidant activity through DPPH assay. These AgNPs also exhibited potential antibacterial activity against human pathogenic bacteria. The results were compared with the antioxidant and antibacterial activities of the plant extract, and clearly suggest that the green biosynthesized AgNPs can constitute an effective antioxidant and antibacterial agent.

scholarly journals Synthesis of cobalt nanowires in aqueous solution under an external magnetic field

2016 ◽  
Vol 7 ◽  
pp. 990-994 ◽  
Author(s):  
Xiaoyu Li ◽  
Lijuan Sun ◽  
Hu Wang ◽  
Kenan Xie ◽  
Qin Long ◽  
...  
Keyword(s):  
Magnetic Field ◽  
Aqueous Solution ◽  
External Magnetic Field ◽  
Room Temperature ◽  
Magnetic Measurements ◽  
Chemical Reduction ◽  
Average Diameter ◽  
Firm Structure ◽  
Cobalt Nanowires ◽  
First Time

In contrast to the majority of related experiments, which are carried out in organic solvents at high temperatures and pressures, cobalt nanowires were synthesized by chemical reduction in aqueous solution with the assistance of polyvinylpyrrolidone (PVP) as surfactant under moderate conditions for the first time, while an external magnetic field of 40 mT was applied. Uniform linear cobalt nanowires with relatively smooth surfaces and firm structure were obtained and possessed an average diameter of about 100 nm with a coating layer of PVP. By comparison, the external magnetic field and PVP were proven to have a crucial influence on the morphology and the size of the synthesized cobalt nanowires. The prepared cobalt nanowires are crystalline and mainly consist of cobalt as well as a small amount of platinum. Magnetic measurements showed that the resultant cobalt nanowires were ferromagnetic at room temperature. The saturation magnetization (M s) and the coercivity (H c) were 112.00 emu/g and 352.87 Oe, respectively.

Biogenic Gold Nanoparticles and its Antibacterial Activities: Houttuynia Cordata Leaf Extract

2014 ◽  
Vol 1051 ◽  
pp. 392-397
Author(s):  
T.V.M. Sreekanth ◽  
In Yong Eom
Keyword(s):  
Gold Nanoparticles ◽  
Leaf Extract ◽  
Antibacterial Activities ◽  
Gram Negative Bacteria ◽  
Gram Positive ◽  
Houttuynia Cordata ◽  
Gram Negative ◽  
Gram Positive Bacteria ◽  
Visible Spectra ◽  
Uv Visible

Gold nanoparticles (AuNPs) can be prepared in a number of chemical techniques, which are not environmentally friendly. Biosynthesis of nanoparticles by plant extracts is currently under exploitation. In this work, we describe an eco-friendly technique for green synthesis of AuNPs from AuCl4 solution using the Houttuynia cordata leaf extract as reducing agent. The AuNPs were characterized using UV-Visible spectroscopy, SEM, TEM, FTIR and AFM. The UV-Visible spectra indicate a strong plasma resonance that is located at 535 nm. The antibacterial activity of AuNPs was performed on various gram positive and gram negative bacteria. The AuNPs showed more inhibitory activity on gram negative than gram positive bacteria.

Toxicity evaluation of gold nanoparticles using an Allium cepa bioassay

RSC Advances ◽  
2016 ◽  
Vol 6 (29) ◽  
pp. 24000-24009 ◽  
Author(s):  
A. Rajeshwari ◽  
S. Suresh ◽  
Natarajan Chandrasekaran ◽  
Amitava Mukherjee
Keyword(s):  
Gold Nanoparticles ◽  
Allium Cepa ◽  
Cost Effective ◽  
Toxicity Evaluation ◽  
Cytogenetic Effects ◽  
Gold Nps ◽  
First Time ◽  
Dose Dependent

The size- and dose-dependent cytogenetic effects of gold NPs were evaluated for the first time by a simple and cost-effective Allium cepa (A. cepa) bioassay.

scholarly journals Synthesis and characterization of zinc and copper oxide nanoparticles and their antibacterial activity

2020 ◽  
Author(s):  
Richard B Asamoah ◽  
Abu Yaya ◽  
Bismark Mensah ◽  
Pascal Nbelayim ◽  
Vitus Apalangya ◽  
...  
Keyword(s):  
Copper Oxide ◽  
Average Length ◽  
Average Diameter ◽  
Xrd Analysis ◽  
Antibacterial Activities ◽  
Copper Oxide Nanoparticles ◽  
Oxide Nanoparticles ◽  
E Coli ◽  
Transmission Electron ◽  
Inhibition Concentration

Inorganic nano-metal oxides can be effective alternatives to drug resistant organic antibiotics due to their broad spectrum antimicrobial activity against pathogenic and mutagenic gram-negative and positive bacteria.  In this study, zinc and copper oxides (ZnO and CuO) were synthesized using a facile wet chemical method. The oxide nanoparticles were characterized using X-ray diffraction (XRD), UV-Vis spectrometer (UV-Vis), Fourier Transformed Infra-red spectrometer and Transmission electron microscopy (TEM). The antibacterial activities of the nanoparticles were investigated against e. coli and s. aureus using the disk diffusion and microdilution tests. The XRD analysis revealed that both zinc and copper oxide nanoparticles were purely crystalline. The TEM micrographs showed that copper oxide nanoparticles assumed a nanorod shape of average length of 100 nm.  Whiles zinc oxide nanoparticles were spherical of average diameter of 15 nm. The FTIR results showed that the nanoparticles were free of impurities and organic surfactants. The optical band gaps of CuO and ZnO according to UV-Vis analysis were respectively 2.63 eV and 3.22 eV. According to the antibacteria tests, the minimum inhibition concentration (MIC) of CuO against e. coli and s. aureus were correspondingly 1mg/ml and 0.25 mg/ml whiles it was 0.1mg/ml for ZnO against s. aureus but ZnO produced no inhibition against e. coli. With the microdilution test, both nanoparticles exhibited activity against both bacteria species at all varying concentrations. CuO had an antibacteria efficiency of 80 to 97% and 85 to 99% for e. coli and s. aureus respectively. The efficiency of ZnO were 20 to 90% and 50 to 89% for e. coli and s. aureus accordingly. The results concluded that CuO had higher antibacteria activity as compared to ZnO.

scholarly journals Biosynthesis of Smaller-Sized Platinum Nanoparticles Using the Leaf Extract of Combretum erythrophyllum and Its Antibacterial Activities

Antibiotics ◽  
2021 ◽  
Vol 10 (11) ◽  
pp. 1275
Author(s):  
Olufunto T. Fanoro ◽  
Sundararajan Parani ◽  
Rodney Maluleke ◽  
Thabang C. Lebepe ◽  
Rajendran J. Varghese ◽  
...  
Keyword(s):  
Platinum Nanoparticles ◽  
Klebsiella Oxytoca ◽  
Particle Diameter ◽  
Cost Effective ◽  
Antibacterial Activities ◽  
Average Particle ◽  
Klebsiella Species ◽  
Green Materials ◽  
Transmission Electron ◽  
Materials Used

Nanobiotechnology is a promising field in the development of safe antibiotics to combat the increasing trend of antibiotic resistance. Nature is a vast reservoir for green materials used in the synthesis of non-toxic and environmentally friendly nano-antibiotics. We present for the first time a facile, green, cost-effective, plant-mediated synthesis of platinum nanoparticles (PtNPs) using the extract of Combretum erythrophyllum (CE) plant leaves. The extract of CE served as both a bio-reductant and a stabilizing agent. The as-synthesized PtNPs were characterized using ultraviolet-visible (UV–Vis) absorption spectroscopy, high-resolution transmission electron microscopy (HR-TEM), Fourier transform infrared spectroscopy (FTIR), X-ray diffraction (XRD), and dynamic light scattering (DLS) techniques. The HR-TEM image confirmed that the PtNPs are ultrasmall, spherical, and well dispersed with an average particle diameter of 1.04 ± 0.26 nm. The PtNPs showed strong antibacterial activities against pathogenic Gram-positive Staphylococcus epidermidis (ATCC 14990) at a minimum inhibitory concentration (MIC) of 3.125 µg/mL and Gram-negative Klebsiella oxytoca (ATCC 8724) and Klebsiella aerogenes (ATCC 27853) at an MIC value of 1.56 µg/mL. The CE-stabilized PtNPs was mostly effective in Klebsiella species that are causative organisms in nosocomial infections.

One Pot Green Synthesis of Gold Nanoparticles Using Piper Betle Leaf Extract and their Antibacterial Activities

2021 ◽  
Vol 1163 ◽  
pp. 106-116
Author(s):  
Harekrishna Bar
Keyword(s):  
Gold Nanoparticles ◽  
Leaf Extract ◽  
Antibacterial Activities ◽  
Diffusion Method ◽  
Piper Betle ◽  
Metallic State ◽  
Antibacterial Drug ◽  
One Pot ◽  
Stabilizing Agents ◽  
E Coli

Gold nanoparticles have been successfully synthesized using aqueous leaf extract of Piper betle In this extracellular synthesis, after exposing of metal ions to betel leaf extract, reduction leads into their metallic state and these are stabilized by the biomolecules present in leaf extract, where extract are being used as both reducing as well as stabilizing agents at ambient condition. Gold (AuNPs) nanoparticles are characterized by UV-Vis, FESEM, HRTEM and XRD measurements. Synthesized gold nanoparticles are mostly spherical in shape with diameter ~ 30-50 nm. Antibacterial activities of the synthesized nanoparticles are investigated against two Gram-negative (Escherichia coli and Pseudomonas aeruginosa) and two Gram-positive (Staphylococcus aureus and Bacillus thuringiensis) bacteria using the disc diffusion method. AuNPs show inhibition activity against P. aeruginosa andE. coli respectively nearly equivalent to the commercially available antibacterial drug e.g. Norfloxacin (Nx). The minimum inhibition concentration (MIC) results indicate that 36 μg/mL gold nanoparticles inhibit the growth of E. coli cells.

Enhanced Magnetoelectricity and Large Phonon Abnormality of Feather-Like CoFe2O4-BaTiO3 Nanostructures

2012 ◽  
Vol 560-561 ◽  
pp. 761-765
Author(s):  
Yu Deng ◽  
Di Wu ◽  
You Wei Du
Keyword(s):  
Growth Mechanism ◽  
Room Temperature ◽  
Hydrothermal Reaction ◽  
Three Dimensional ◽  
Cost Effective ◽  
Average Diameter

By cost-effective techniques of hydrothermal reaction and polymer-assisted deposition, Feather-like (CoFe2O4)0.3-(BaTiO3)0.7 (CFO-BTO) nanostructures have been synthesized. The nanostructures show an average diameter of 250 nm and lengths up to 5 μm, with CFO nanopillars embedded in BTO matrix. The three-dimensional microstructure and growth mechanism of the nanostructures have been investigated. A large magnetoelectric (ME) response at room-temperature and a strong phonon abnormality between 110 °C and 140 °C have been studied.

scholarly journals Designing and optimization of an electrochemical substitute for the MTT (3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) cell viability assay

2019 ◽  
Vol 9 (1) ◽  
Author(s):  
Mohammad Mazloum-Ardakani ◽  
Behnaz Barazesh ◽  
Seyed Mohammad Moshtaghioun ◽  
Mohammad Hasan Sheikhha
Keyword(s):  
Cell Viability ◽  
Cost Effective ◽  
The Novel ◽  
Electrochemical Assay ◽  
Cell Viability Assay ◽  
Cytotoxic Effects ◽  
Viability Assay ◽  
Toxic Materials ◽  
Diphenyltetrazolium Bromide ◽  
First Time

Abstract For the first time ever, this paper reports the development of an easily operated and cost-effective electrochemical assay to be used as an appropriate substitute for the MTT (3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) cell viability assay. The proposed assay is based on the electrochemical reaction of Saccharomyces cerevisiae (S. cerevisiae) with toxic materials, and it overcomes most of the limitations of MTT such as evaporation of volatile solvents, cytotoxic effects of MTT reagents, high cost, and sensitivity to light. The novel electrochemical assay can be used to detect diazinon in the range of 10−6 g mL−1 to 10−2 g mL−1 with the detection limit of 1.5 × 10−7 g mL−1.

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