scholarly journals Differential Expression and Localization of EHBP1L1 during the First Wave of Rat Spermatogenesis Suggest Its Involvement in Acrosome Biogenesis

Biomedicines ◽  
2022 ◽  
Vol 10 (1) ◽  
pp. 181
Author(s):  
Massimo Venditti ◽  
Sergio Minucci
Keyword(s):  
Molecular Mechanisms ◽  
Post Partum ◽  
Spatial Localization ◽  
Vesicular Trafficking ◽  
Perinuclear Cytoplasm ◽  
Temporal And Spatial ◽  
Rat Spermatogenesis ◽  
Identification And Characterization ◽  
Combined Data

The identification and characterization of new proteins involved in spermatogenesis is fundamental, considering that good-quality gametes are basic in ensuring proper reproduction. Here, we further analyzed the temporal and spatial localization during the first spermatogenic wave of rat testis of EHBP1L1, which is involved in vesicular trafficking due to the CH and bMERB domains, which bind to actin and Rab8/10, respectively. Western blot and immunofluorescence analyses showed that EHBP1L1 protein expression started at 21 days post-partum (dpp) concomitantly with the appearance of primary spermatocytes (I SPC). In subsequent stages, EHBP1L1 specifically localized together with actin in the perinuclear cytoplasm close to the acrosomal and Golgian regions of spermatids (SPT) during the different phases of acrosome biogenesis (AB). Moreover, it was completely absent in elongated SPT and in mature spermatozoa, suggesting that its role was completed in previous stages. The combined data, also supported by our previous report demonstrating that EHBP1L1 mRNA was expressed by primary (I) and secondary (II) SPC, lead us to hypothesize its specific role during AB. Although these results are suggestive, further studies are needed to better clarify the underlying molecular mechanisms of AB, with the aim to use EHBP1L1 as a potential new marker for spermatogenesis.

scholarly journals EARLY BUD-BREAK 1 and EARLY BUD-BREAK 3 control resumption of poplar growth after winter dormancy

2021 ◽  
Vol 12 (1) ◽  
Author(s):  
Abdul Azeez ◽  
Yiru Chen Zhao ◽  
Rajesh Kumar Singh ◽  
Yordan S. Yordanov ◽  
Madhumita Dash ◽  
...  
Keyword(s):  
Cell Cycle ◽  
Molecular Mechanisms ◽  
Negative Regulator ◽  
Bud Break ◽  
Positive Regulator ◽  
Regulatory Module ◽  
Trees And Shrubs ◽  
Poplar Growth ◽  
Identification And Characterization

AbstractBud-break is an economically and environmentally important process in trees and shrubs from boreal and temperate latitudes, but its molecular mechanisms are poorly understood. Here, we show that two previously reported transcription factors, EARLY BUD BREAK 1 (EBB1) and SHORT VEGETATIVE PHASE-Like (SVL) directly interact to control bud-break. EBB1 is a positive regulator of bud-break, whereas SVL is a negative regulator of bud-break. EBB1 directly and negatively regulates SVL expression. We further report the identification and characterization of the EBB3 gene. EBB3 is a temperature-responsive, epigenetically-regulated, positive regulator of bud-break that provides a direct link to activation of the cell cycle during bud-break. EBB3 is an AP2/ERF transcription factor that positively and directly regulates CYCLIND3.1 gene. Our results reveal the architecture of a putative regulatory module that links temperature-mediated control of bud-break with activation of cell cycle.

Abstract PR-008: Identification and characterization of the molecular mechanisms of SCLC chemo-radiation resistance

2021 ◽  
Author(s):  
Francesca Anna Carrieri ◽  
Nick Connis ◽  
Eloise Grasset ◽  
Eddie Luidy-Imada ◽  
Andrew Ewald ◽  
...  
Keyword(s):  
Radiation Resistance ◽  
Molecular Mechanisms ◽  
Identification And Characterization

Identification and Characterization of Rice Circular RNAs Responding to Xanthomonas Oryzae pv. Oryzae Invasion

2021 ◽  
Author(s):  
Peihong Wang ◽  
Sai Wang ◽  
Yan Wu ◽  
Wenhan Nie ◽  
Ayizekeranmu Yiming ◽  
...  
Keyword(s):  
Molecular Mechanisms ◽  
Expression Profiles ◽  
Circular Rnas ◽  
Pathogen Invasion ◽  
Rice Leaves ◽  
Transcriptional Gene Regulation ◽  
Identification And Characterization ◽  
Host Genes

Abstract BackgroundThe emerging role of circular RNAs (circRNAs) in various biological processes have advanced our knowledge of transcriptional and post-transcriptional gene regulation. The number and expression of plant circRNAs vary with species and treatments. However, the expression profile and the potential role of circRNAs during plant response to pathogen invasion are still elusive. ResultsIn this study, we identified 3517 circRNAs from PXO99A-infected rice leaves using the ribosomal RNA (rRNA) depleted RNA-Sequencing technique coupled with the CIRI2 and CIRCexplorer2 pipeline. Among them, 2994 (85.13%) circRNAs arised from the exons of their parent genes, 1214 circRNAs were previously unknown and 276 circRNAs exhibited differential expression profiles upon PXO99A infection over time. In addition, 31 differentially expressed circRNAs (DEcircRNAs) were predicted as the corresponding 121 miRNAs sponges. Functional analysis of both host genes and target mRNAs suggested that these identified circRNAs might play an important role in reprogramming rice responses to PXO99A invasion, mainly by mediating photorespiration, chloroplast, peroxisome and diterpenoid biosynthesis associated pathways.ConclusionThese results inferred a potential functional role of circRNAs in the regulation of rice immunity and provide novel clues for revealing the molecular mechanisms of rice-PXO99A interaction.

Identification and characterization of microRNAs in white and black coated yak

2018 ◽  
Author(s):  
Wang-Dui Basang ◽  
Tian-Wu An ◽  
Xiao-Lin Luo ◽  
Yan-Bin Zhu ◽  
Luo-Bu Danjiu Danjiu ◽  
...  
Keyword(s):  
Molecular Mechanisms ◽  
Coat Color ◽  
Wnt Signaling Pathway ◽  
Differentially Expressed ◽  
Black And White ◽  
Kegg Pathways ◽  
Differentially Expressed Mirnas ◽  
Paired End Sequencing ◽  
Identification And Characterization

In this study, we used high-throughput technology to provide the first transcriptome dataset for differentially expressed miRNA in mixed pools of dermis tissue from black- and white-coated yak to research the possible molecular mechanisms of yak coat pigmentation. In this study, 92,636,002 and 95,917,842 clear reads were generated through Illumina paired-end sequencing. A total of 78 differentially expressed miRNAs (DEMs) were identified, including 59 upregulated and 19 downregulated miRNAs in the mixed pools of white-coated yak compared with the mixed pools of black-coated yak. In addition, 3634 genes were predicted as putative targets of DEMs. These DEGs related to 59 GO categories and were enriched in 216 Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways, including melanogenesis and the Wnt signaling pathway. The results of the current study indicated that the coat color of the yak involved the transcriptional regulation process of miRNAs. These results provide helpful data to understand the molecular mechanisms of yak coat pigmentation.

SAT0043 Identification and Characterization of Novel Molecular Mechanisms for ACPA-Driven Osteoclastogenesis

2015 ◽  
Vol 74 (Suppl 2) ◽  
pp. 663.3-664
Author(s):  
A. Krishnamurthy ◽  
V. Joshua ◽  
K. Amara ◽  
C. Cerqueira ◽  
K. Lundberg ◽  
...  
Keyword(s):  
Molecular Mechanisms ◽  
Identification And Characterization

scholarly journals Identification and Characterization of a Linear-Plasmid-Encoded Factor H-Binding Protein (FhbA) of the Relapsing Fever Spirochete Borrelia hermsii

2004 ◽  
Vol 186 (9) ◽  
pp. 2612-2618 ◽  
Author(s):  
Kelley M. Hovis ◽  
John V. McDowell ◽  
LaToya Griffin ◽  
Richard T. Marconi
Keyword(s):  
Molecular Mechanisms ◽  
Binding Protein ◽  
Genetic Locus ◽  
Factor H ◽  
Linear Plasmid ◽  
Relapsing Fever ◽  
Gene Encoding ◽  
Borrelia Hermsii ◽  
Identification And Characterization

ABSTRACT In North America, tick-borne relapsing fever (TBRF) is caused by the spirochete species Borrelia hermsii, Borrelia parkeri, and Borrelia turicatae. We previously demonstrated that some isolates of B. hermsii and B. parkeri are capable of binding factor H and that cell-bound factor H can participate in the factor I-mediated cleavage of C3b. Isolates that bound factor H expressed a factor H-binding protein (FHBP) that we estimated to be approximately 19 to 20 kDa in size and thus, pending further characterization, temporarily designated FHBP19. Until this report, none of the FHBPs of the TBRF spirochetes had been characterized. Here we have recovered the gene encoding the FHBP of B. hermsii YOR from a lambda ZAP II library and determined its sequence. The gene encodes a full-length protein of 22.7 kDa, which after processing is predicted to be 20.5 kDa. This protein, which we redesignate factor H-binding protein A (FhbA), is unique to B. hermsii. Two-dimensional pulsed-field gel electrophoresis and hybridization analyses revealed that the B. hermsii gene encoding FhbA is a single genetic locus that maps to a linear plasmid of approximately 220 kb. The general properties of FhbA were also assessed. The protein was found to be surface exposed and lipidated. Analysis of the antibody response to FhbA in infected mice revealed that it is antigenic during infection, indicating expression during infection. The identification and characterization of FhbA provides further insight into the molecular mechanisms of pathogenesis of the relapsing fever spirochetes.

Identification and characterization of rice circular RNAs responding to Xanthomonas oryzae pv. oryzae invasion

2021 ◽  
Author(s):  
Peihong Wang ◽  
Sai Wang ◽  
Yan Wu ◽  
Wenhan Nie ◽  
Ayizekeranmu Yiming ◽  
...  
Keyword(s):  
Molecular Mechanisms ◽  
Enrichment Analysis ◽  
Xanthomonas Oryzae ◽  
Pcr Analysis ◽  
Circular Rnas ◽  
Transcriptional Gene Regulation ◽  
Identification And Characterization ◽  
Host Genes

Emerging role of circular RNAs (circRNAs) in various biological processes have advanced our knowledge of transcriptional and post-transcriptional gene regulation. To date, no research has been conducted to explore their roles in the rice- Xanthomonas oryzae pv. oryzae (Xoo) interaction. Therefore, we identified 3517 circRNAs from the highly virulent Xoo strain PXO99A-infected rice leaves using the ribosomal RNA (rRNA) depleted RNA-sequencing technique coupled with the CIRI2 and CIRCexplorer2 pipeline. Characterization analyses showed that these circRNAs were distributed across the whole genome of rice, and most circRNAs arised from exons (85.13 %), ranged from 200 bp to 1000 bp and were with a non-canonical GT/AG (including CT/AC equivalent) splicing signal. Functional annotation and enrichment analysis of the host genes that produced the DEcircRNAs suggested that these identified circRNAs might play an important role in reprogramming rice responses to PXO99A invasion, mainly by mediating photorespiration, chloroplast, peroxisome and diterpenoid biosynthesis. Moreover, 31 differentially expressed circRNAs (DEcircRNAs) were predicted to act as miRNA decoys in rice. The expression profile of 4 DEcircRNAs were validated by RT-qPCR with divergent primers, and the back-splicing sites of seven DEcircRNAs were verified by PCR analysis and Sanger sequencing. Collectively, these results inferred a potential functional role of circRNAs in the regulation of rice immunity and provide novel clues for revealing the molecular mechanisms of rice-PXO99A interaction.

scholarly journals PTPN11 is the first identified proto-oncogene that encodes a tyrosine phosphatase

Blood ◽  
2006 ◽  
Vol 109 (3) ◽  
pp. 862-867 ◽  
Author(s):  
Rebecca J. Chan ◽  
Gen-Sheng Feng
Keyword(s):  
Tumor Suppressor Genes ◽  
Molecular Mechanisms ◽  
Cell Transformation ◽  
Tyrosine Phosphatase ◽  
Research Field ◽  
Sh2 Domains ◽  
Src Homology 2 ◽  
Src Homology ◽  
Identification And Characterization

Abstract Elucidation of the molecular mechanisms underlying carcinogenesis has benefited tremendously from the identification and characterization of oncogenes and tumor suppressor genes. One new advance in this field is the identification of PTPN11 as the first proto-oncogene that encodes a cytoplasmic tyrosine phosphatase with 2 Src-homology 2 (SH2) domains (Shp2). This tyrosine phosphatase was previously shown to play an essential role in normal hematopoiesis. More recently, somatic missense PTPN11 gain-of-function mutations have been detected in leukemias and rarely in solid tumors, and have been found to induce aberrant hyperactivation of the Ras-Erk pathway. This progress represents another milestone in the leukemia/cancer research field and provides a fresh view on the molecular mechanisms underlying cell transformation.

scholarly journals Identification and characterization of Gbeta3s2, a novel splice variant of the G-protein beta3 subunit

2003 ◽  
Vol 371 (1) ◽  
pp. 223-232 ◽  
Author(s):  
Dieter ROSSKOPF ◽  
Iris MANTHEY ◽  
Christiane HABICH ◽  
Marzena KIELBIK ◽  
Andreas EISENHARDT ◽  
...  
Keyword(s):  
G Protein ◽  
Splice Variant ◽  
Mammalian Cells ◽  
Molecular Mechanisms ◽  
Biologically Active ◽  
Complex Phenotype ◽  
825T Allele ◽  
Propeller Blades ◽  
Identification And Characterization

The T-allele of a polymorphism (C825T) in the gene for the G-protein β3 subunit (GNB3) is associated with cardiovascular and metabolic disorders, distinct cellular features and altered drug responses. The molecular mechanisms that give rise to this complex phenotype have been linked to the occurrence of Gβ3s, a splice variant of GNB3. Gβ3s is predominantly expressed in cells with the 825T-allele. In the present study we describe the identification and characterization of an additional Gβ3 splice variant referred to as Gβ3s2. Its mRNA is expressed in heart, blood cells and tumour tissue, and its expression is also tightly associated with the GNB3 825T-allele. Gβ3s2 is generated by alternative splicing using non-canonical splice sites. Gβ subunits belong to the family of propeller proteins and consist of seven regular propeller blades. Transcripts for Gβ3s2 are lacking 129bp of the coding sequence of the wild-type Gβ3 protein. Thus the predicted structure consists of only six propeller blades, which resembles the structure of Gβ3s. Co-immunoprecipitation analyses indicated that Gβ3s2 dimerizes with different Gγ subunits, e.g. Gγ5, Gγ8C and Gγ12. In Sf9 insect cells, expression of Gβ3s2 together with Gγ12 enhances receptor-stimulated activation of Gαi2. Expression of Gβ3s2 in mammalian cells activated the mitogen-activated protein kinase cascade. Together, these results suggest that Gβ3s2 is a biologically active Gβ variant which may play a role in the manifestation of the complex phenotype associated with the 825T-allele.

scholarly journals Proteomic Analysis of Phagocytosis in the Enteric Protozoan Parasite Entamoeba histolytica

2005 ◽  
Vol 4 (4) ◽  
pp. 827-831 ◽  
Author(s):  
Mami Okada ◽  
Christopher D. Huston ◽  
Barbara J. Mann ◽  
William A. Petri ◽  
Kiyoshi Kita ◽  
...  
Keyword(s):  
Entamoeba Histolytica ◽  
Proteomic Analysis ◽  
Protozoan Parasite ◽  
Immunofluorescence Assay ◽  
Vesicular Trafficking ◽  
Surface Recognition ◽  
Identification And Characterization ◽  
Enteric Protozoan ◽  
Cytoskeleton Rearrangement

ABSTRACT Proteomic analysis of phagosomes isolated from Entamoeba histolytica by liquid chromatography and mass spectrometry identified 85 proteins involved in surface recognition, actin cytoskeleton rearrangement, vesicular trafficking, and degradation. Phagosome localization of representative proteins was verified by immunofluorescence assay. This study should provide a basis for molecular identification and characterization of phagosome biogenesis.

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