Cancer-Specific Biomarker hNQO1-Activatable Fluorescent Probe for Imaging Cancer Cells In Vitro and In Vivo

Human NAD(P)H quinone oxidoreductase-1 (hNQO1) is an important cancer-related biomarker, which shows significant overexpression in malignant cells. Developing an effective method for detecting NQO1 activity with high sensitivity and selectivity in tumors holds a great potential for cancer diagnosis, treatment, and management. In the present study, we report a new dicyanoisophorone (DCP) based fluorescent probe (NQ-DCP) capable of monitoring hNQO1 activity in vitro and in vivo in both ratiometric and turn-on model. NQ-DCP was prepared by conjugating dicyanoisophorone fluoroprobe with hNQO1 activatable quinone propionic acid (QPA), which remain non-fluorescent until activation by tumor-specific hNQO1. NQ-DCP featured a large Stokes shift (145 nm), excellent biocompatibility, cell permeability, and selectivity towards hNQO1 allowed to differentiate cancer cells from healthy cells. We have successfully employed NQ-DCP to monitor non-invasive endogenous hNQO1 activity in brain tumor cells in vitro and in xenografted tumors developed in nude mice.

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Synthesis of an MOF-based Hg2+-fluorescent probe via stepwise post-synthetic modification in a single-crystal-to-single-crystal fashion and its application in bioimaging

Dalton Transactions ◽

10.1039/c9dt02866h ◽

2019 ◽

Vol 48 (44) ◽

pp. 16502-16508 ◽

Cited By ~ 2

Author(s):

Wen-Yan Li ◽

Song Yang ◽

Yan-An Li ◽

Qian-Ying Li ◽

Qun Guan ◽

...

Keyword(s):

Single Crystal ◽

Fluorescent Probe ◽

Fluorescent Sensor ◽

High Sensitivity ◽

Sensitivity And Selectivity

A new MOF-based Hg2+ fluorescent sensor, UiO-68-R6G, was prepared via successive single-crystal-to-single-crystal post-synthetic modification; this sensor could detect Hg2+ both in vitro and in vivo with high sensitivity and selectivity.

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Aminoluciferin 4-hydroxyphenyl amide enables bioluminescence detection of endogenous tyrosinase

Organic & Biomolecular Chemistry ◽

10.1039/c8ob01777h ◽

2018 ◽

Vol 16 (47) ◽

pp. 9197-9203 ◽

Cited By ~ 2

Author(s):

Chunchao Tang ◽

Lei Jin ◽

Yuxing Lin ◽

Jing Su ◽

Yingai Sun ◽

...

Keyword(s):

High Sensitivity ◽

Living Cells ◽

Tyrosinase Activity ◽

Cell Permeability ◽

Sensitivity And Selectivity ◽

Good Cell

We report a new BL probe,TyrBP-3, which not only exhibits high sensitivity and selectivity for imaging tyrosinase in vitro, and good cell-permeability for detecting tyrosinase in living cells, but can also visualize the level of tyrosinase activity in tumors of living animals.

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A near-infrared and two-photon ratiometric fluorescent probe with a large Stokes shift for sulfur dioxide derivatives detection and its applications in vitro and in vivo

Sensors and Actuators B Chemical ◽

10.1016/j.snb.2019.01.170 ◽

2019 ◽

Vol 288 ◽

pp. 519-526 ◽

Cited By ~ 14

Author(s):

Yuping Zhao ◽

Yanyan Ma ◽

Weiying Lin

Keyword(s):

Sulfur Dioxide ◽

Fluorescent Probe ◽

Near Infrared ◽

Stokes Shift ◽

Large Stokes Shift ◽

Two Photon ◽

Ratiometric Fluorescent Probe

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Label-Free Split Aptamer Sensor for Femtomolar Detection of Dopamine by Means of Flexible Organic Electrochemical Transistors

Materials ◽

10.3390/ma13112577 ◽

2020 ◽

Vol 13 (11) ◽

pp. 2577 ◽

Cited By ~ 5

Author(s):

Yuanying Liang ◽

Ting Guo ◽

Lei Zhou ◽

Andreas Offenhäusser ◽

Dirk Mayer

Keyword(s):

Detection Limit ◽

Electrochemical Sensors ◽

Limit Of Detection ◽

High Sensitivity ◽

Label Free ◽

Dopamine Detection ◽

Electrochemical Transistor ◽

Sensitivity And Selectivity

The detection of chemical messenger molecules, such as neurotransmitters in nervous systems, demands high sensitivity to measure small variations, selectivity to eliminate interferences from analogues, and compliant devices to be minimally invasive to soft tissue. Here, an organic electrochemical transistor (OECT) embedded in a flexible polyimide substrate is utilized as transducer to realize a highly sensitive dopamine aptasensor. A split aptamer is tethered to a gold gate electrode and the analyte binding can be detected optionally either via an amperometric or a potentiometric transducer principle. The amperometric sensor can detect dopamine with a limit of detection of 1 μM, while the novel flexible OECT-based biosensor exhibits an ultralow detection limit down to the concentration of 0.5 fM, which is lower than all previously reported electrochemical sensors for dopamine detection. The low detection limit can be attributed to the intrinsic amplification properties of OECTs. Furthermore, a significant response to dopamine inputs among interfering analogues hallmarks the selective detection capabilities of this sensor. The high sensitivity and selectivity, as well as the flexible properties of the OECT-based aptasensor, are promising features for their integration in neuronal probes for the in vitro or in vivo detection of neurochemical signals.

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Nanomaterials-Based Electrochemical Sensors for In Vitro and In Vivo Analyses of Neurotransmitters

Applied Sciences ◽

10.3390/app8091504 ◽

2018 ◽

Vol 8 (9) ◽

pp. 1504 ◽

Cited By ~ 17

Author(s):

Sharmila Durairaj ◽

Boopathi Sidhureddy ◽

Joseph Cirone ◽

Aicheng Chen

Keyword(s):

Electrochemical Sensors ◽

Point Of Care ◽

Electrochemical Techniques ◽

High Sensitivity ◽

Electrochemical Analysis ◽

Detection Techniques ◽

Efficient Detection ◽

Sensitivity And Selectivity

Neurotransmitters are molecules that transfer chemical signals between neurons to convey messages for any action conducted by the nervous system. All neurotransmitters are medically important; the detection and analysis of these molecules play vital roles in the diagnosis and treatment of diseases. Among analytical strategies, electrochemical techniques have been identified as simple, inexpensive, and less time-consuming processes. Electrochemical analysis is based on the redox behaviors of neurotransmitters, as well as their metabolites. A variety of electrochemical techniques are available for the detection of biomolecules. However, the development of a sensing platform with high sensitivity and selectivity is challenging, and it has been found to be a bottleneck step in the analysis of neurotransmitters. Nanomaterials-based sensor platforms are fascinating for researchers because of their ability to perform the electrochemical analysis of neurotransmitters due to their improved detection efficacy, and they have been widely reported on for their sensitive detection of epinephrine, dopamine, serotonin, glutamate, acetylcholine, nitric oxide, and purines. The advancement of electroanalytical technologies and the innovation of functional nanomaterials have been assisting greatly in in vivo and in vitro analyses of neurotransmitters, especially for point-of-care clinical applications. In this review, firstly, we focus on the most commonly employed electrochemical analysis techniques, in conjunction with their working principles and abilities for the detection of neurotransmitters. Subsequently, we concentrate on the fabrication and development of nanomaterials-based electrochemical sensors and their advantages over other detection techniques. Finally, we address the challenges and the future outlook in the development of electrochemical sensors for the efficient detection of neurotransmitters.

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A novel near-infrared fluorescent probe with a large Stokes shift for biothiol detection and application in in vitro and in vivo fluorescence imaging

Journal of Materials Chemistry B ◽

10.1039/c7tb00187h ◽

2017 ◽

Vol 5 (21) ◽

pp. 3836-3841 ◽

Cited By ~ 35

Author(s):

Keyin Liu ◽

Huiming Shang ◽

Xiuqi Kong ◽

Weiying Lin

Keyword(s):

Fluorescent Probe ◽

Fluorescence Imaging ◽

Near Infrared ◽

Stokes Shift ◽

Large Stokes Shift ◽

In Vivo Fluorescence ◽

In Vivo Fluorescence Imaging

A novel near-infrared fluorescent probe with a large Stokes shift was developed for biothiol detection and application in in vitro and in vivo fluorescence imaging.

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