scholarly journals 3D Multicellular Stem-Like Human Breast Tumor Spheroids Enhance Tumorigenicity of Orthotopic Xenografts in Athymic Nude Rat Model

Cancers ◽  
2021 ◽  
Vol 13 (11) ◽  
pp. 2784
Author(s):  
Reza Bayat Mokhtari ◽  
Bessi Qorri ◽  
Manpreet Sambi ◽  
Narges Baluch ◽  
Sushil Kumar ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Stem Cells ◽  
Stem Cell ◽  
Rat Model ◽  
Breast Tumor ◽  
Stem Cell Factor ◽  
Human Breast ◽  
Human Breast Tumor ◽  
Nude Rat ◽  
Orthotopic Xenografts

Therapeutic targeting of stem cells needs to be strategically developed to control tumor growth and prevent metastatic burden successfully. Breast cancer presents a unique clinical problem because of the variety of cellular subtypes present, including cancer stem cells (CSCs). The development of 3D stem-like properties of human breast tumor spheroids in stem cell factor conditioned media was investigated in orthotopic xenografts for enhanced tumorgenicity in the athymic nude rat model. MCF-7, ZR-75-1, and MDA-MB-231 breast cancer cell lines were cultured in serum-free, stem cell factor-supplemented medium under non-adherent conditions and passaged to generate 3rd generation spheroids. The spheroids were co-cultured with fetal lung fibroblast (FLF) cells before orthotopic heterotransplantation into the mammary fat pads of athymic nude rats. Excised xenografts were assessed histologically by H&E staining and immunohistochemistry for breast cancer marker (ERB1), proliferation marker (Ki67), mitotic marker (pHH3), hypoxia marker (HIF-2α), CSC markers (CD47, CD44, CD24, and CD133), and vascularization markers (CD31, CD34). Breast cancer cells cultured in stem cell factor supplemented medium generated 3D spheroids exhibited increased stem-like characteristics. The 3D stem-like spheroids co-cultured with FLF as supporting stroma reproducibly and efficiently established orthotopic breast cancer xenografts in the athymic nude rat.

Adipose-derived stem cells enhance human breast cancer growth and cancer stem cell-like properties through adipsin

Oncogene ◽  
2018 ◽  
Vol 38 (6) ◽  
pp. 767-779 ◽  
Author(s):  
Hideaki Goto ◽  
Yohei Shimono ◽  
Yohei Funakoshi ◽  
Yoshinori Imamura ◽  
Masanori Toyoda ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Stem Cells ◽  
Stem Cell ◽  
Cancer Stem Cell ◽  
Human Breast Cancer ◽  
Cancer Growth ◽  
Adipose Derived Stem Cells ◽  
Human Breast ◽  
Breast Cancer Growth

Development of a novel explant culture method for the isolation of mesenchymal stem cells from human breast tumor

2018 ◽  
Vol 39 (2) ◽  
pp. 207-217 ◽  
Author(s):  
Koushan Sineh Sepehr ◽  
Alireza Razavi ◽  
Mohsen Saeidi ◽  
Majid Mossahebi-Mohammadi ◽  
Meghdad Abdollahpour-Alitappeh ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Breast Tumor ◽  
Culture Method ◽  
Explant Culture ◽  
Human Breast ◽  
Human Breast Tumor

GGNBP2 to suppress tumor growth and cancer stem cell load of triple negative breast cancer by controlling STAT3 pathway.

2017 ◽  
Vol 35 (15_suppl) ◽  
pp. e23003-e23003
Author(s):  
Jin Zhang ◽  
Yunhui Hu ◽  
Zhenmin Lei
Keyword(s):  
Breast Cancer ◽  
Gene Expression ◽  
Cell Cycle ◽  
Flow Cytometry ◽  
Stem Cell ◽  
Cancer Stem Cell ◽  
Western Blot ◽  
Breast Tumor ◽  
Triple Negative ◽  
Human Breast

e23003 Background: Gametogenetin binding protein 2 (GGNBP2) is encoded in human chromosome 17q12-q23, a region known as a breast and ovarian cancer susceptibility locus. GGNBP2 has a single C2H2 zinc finger and a consensus LxxLL nuclear receptor (NR) binding motif. We have reported that GGNBP2 suppresses ERα-positive breast tumorigenesis by acting as a nuclear receptor co-repressor to restrain ERα activity. However, the detailed molecular mechanisms of GGNBP2 and its role in triple negative breast cancer (TNBC) remain largely unclear. Methods: A human breast cancer tissue array containing 138 human breast tumor tissues were utilized to examine GGNBP2 expression in breast cancer samples by IHC. To address the potential anti-breast tumor activity of GGNBP2 in vitro, we expressed exogenous GGNBP2 in TNBC cells, including MDA-MB-231 and Cal51 cell lines. Cell proliferation and cell cycle were assessed by cell growth curve/EdU assays and flow cytometry after propidium Iodide staining. Apoptosis was determined by flow cytometry after annexin V staining, by caspase 3/7 and caspase 9 activity assays. Cancer stem cell properties were determined by expression of CD44/CD24/ALDH1 markers. The levels of phosphorylated STAT3 and total STAT3 were determined by western blot. Quantitative PCR and Western blot were carried out to evaluate the effects of GGNBP2 overexpression on STAT3 target genes, CCND1, Mcl-1, survivin, bax and bim expression. Results: GGNBP2 expression is down-regulated in TNBC cells and patient tumors. GGNBP2 down-regulated expression is associated with poor patient survival. Overexpression of GGNBP2 significanly induces cell cycle G0/G1 phase arrest and apoptosis in TNBC cell lines. Expression of cancer stem cell markers also decreased in GGNBP2-overexpressed TNBC cells. GGNBP2 reduces the expression levels of CCND1, Mcl-1 and survivin, promotes the expression levels of bax and bim proteins. Importantly, overexpression of GGNBP2 inhibits STAT3 phosphorylation and STAT3 downstream target gene expression, including CCND1, Mcl-1 and survivin. Conclusions: GGNBP2 serves as a critical nuclear negative regulator of STAT3-mediated gene expression and tumorigenesis in TNBC.

Comparative immunomodulatory properties of mesenchymal stem cells derived from human breast tumor and normal breast adipose tissue

2020 ◽  
Vol 69 (9) ◽  
pp. 1841-1854
Author(s):  
Koushan Sineh Sepehr ◽  
Alireza Razavi ◽  
Zuhair Mohammad Hassan ◽  
Abdolreza Fazel ◽  
Meghdad Abdollahpour-Alitappeh ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Adipose Tissue ◽  
Breast Tumor ◽  
Normal Breast ◽  
Human Breast ◽  
Human Breast Tumor ◽  
Breast Adipose Tissue ◽  
Breast Adipose ◽  
Immunomodulatory Properties

scholarly journals An unsupervised MVA method to compare specific regions in human breast tumor tissue samples using ToF-SIMS

The Analyst ◽  
2016 ◽  
Vol 141 (6) ◽  
pp. 1947-1957 ◽  
Author(s):  
Blake M. Bluestein ◽  
Fionnuala Morrish ◽  
Daniel J. Graham ◽  
Jamie Guenthoer ◽  
David Hockenbery ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Breast Tumor ◽  
Tumor Tissue ◽  
Regions Of Interest ◽  
Human Breast ◽  
Human Breast Tumor ◽  
Tissue Samples ◽  
Breast Tumor Tissue ◽  
Tof Sims ◽  
Selection Of

PCA-generated regions of interest (ROI) selection of imaging ToF-SIMS data allows chemical comparison of multiple breast cancer specimens.

Abstract 1220: Investigation of the biological properties of human breast cancer in a nude rat model

2014 ◽  
Author(s):  
Reza Bayat Mokhtari ◽  
Joris Tchouala Nofiele ◽  
Syed S. Islam ◽  
Herman Yeger ◽  
Hai-Ling Margaret Cheng
Keyword(s):  
Breast Cancer ◽  
Rat Model ◽  
Human Breast Cancer ◽  
Biological Properties ◽  
Human Breast ◽  
Nude Rat

scholarly journals Thymic stromal lymphopoietin fosters human breast tumor growth by promoting type 2 inflammation

2011 ◽  
Vol 208 (3) ◽  
pp. 479-490 ◽  
Author(s):  
Alexander Pedroza-Gonzalez ◽  
Kangling Xu ◽  
Te-Chia Wu ◽  
Caroline Aspord ◽  
Sasha Tindle ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Tumor Growth ◽  
Breast Tumor ◽  
Tumor Development ◽  
Thymic Stromal Lymphopoietin ◽  
Human Breast ◽  
Human Breast Tumor ◽  
Interleukin 13 ◽  
Th2 Inflammation

The human breast tumor microenvironment can display features of T helper type 2 (Th2) inflammation, and Th2 inflammation can promote tumor development. However, the molecular and cellular mechanisms contributing to Th2 inflammation in breast tumors remain unclear. Here, we show that human breast cancer cells produce thymic stromal lymphopoietin (TSLP). Breast tumor supernatants, in a TSLP-dependent manner, induce expression of OX40L on dendritic cells (DCs). OX40L+ DCs are found in primary breast tumor infiltrates. OX40L+ DCs drive development of inflammatory Th2 cells producing interleukin-13 and tumor necrosis factor in vitro. Antibodies neutralizing TSLP or OX40L inhibit breast tumor growth and interleukin-13 production in a xenograft model. Thus, breast cancer cell–derived TSLP contributes to the inflammatory Th2 microenvironment conducive to breast tumor development by inducing OX40L expression on DCs.

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