scholarly journals Applied Voltage Effect in Lbl Sensors While Detecting 17α-Ethinylestradiol in Water Samples

2021 ◽  
Vol 5 (1) ◽  
pp. 57
Author(s):  
Paulo M. Zagalo ◽  
Cátia Magro ◽  
Paulo A. Ribeiro ◽  
Maria Raposo

The effect of the applied voltage on impedance spectra, measured on sensors based on solid supports with interdigitated electrodes (IDE) that are either covered or not with a layer-by-layer film prepared with polyethylenimine and poly (sodium 4-styrenesulfonate), was analyzed to detect 17α-ethinylestradiol(EE2) in mineral water and tap water. The results show that the sensor response is strongly affected by the applied voltage, the presence of film, and the water matrix, meaning that electrochemical reactions develop near the IDE. However, for low values of applied voltage, the sensor response is reproducible with negligible electrochemical reactions, allowing us to conclude that 25 mV is the appropriate voltage.

2007 ◽  
Vol 50 (1) ◽  
pp. 1-9 ◽  
Author(s):  
Mirian Ueda Yamaguchi ◽  
Rita de Cássia Pontello Rampazzo ◽  
Sueli Fumie Yamada-Ogatta ◽  
Celso Vataru Nakamura ◽  
Tânia Ueda-Nakamura ◽  
...  

The main objective of this study was to analyse the occurrence of yeasts and filamentous fungi in drinking water as well as to investigate their correlation with the indicator bacteria of faecal pollution. Yeasts were detected in 36.6% and 11.6% of the bottled mineral on water dispensers and tap water samples from municipal system, respectively. Twenty-one (35.0%) of bottled mineral water and two (3.3%) of tap water samples were positive for filamentous fungi. For bottled mineral water 12 (20.0%) of 60 samples were positive for total coliform, compared with 3(5.0%)out of 60 samples from tap water. The mineral water from dispensers was more contaminated than tap water. Strains belonging to the genera Candida identified to the species level were C. parapsilosis, C. glabrata and C. albicans. Thus, bottled mineral water from water dispensers and tap water could be considered a possible transmission route for filamentous fungi and yeasts, and could constitute a potential health hazard, mainly to immunocompromised indivuals.


2012 ◽  
Vol 2012 ◽  
pp. 1-10 ◽  
Author(s):  
Azrina Azlan ◽  
Hock Eng Khoo ◽  
Mohd Aizat Idris ◽  
Amin Ismail ◽  
Muhammad Rizal Razman

The drinking and mineral water samples obtained from different geographical locations had concentrations of the selected minerals lower than the standard limits, except for manganese, arsenic, and fluoride. The concentrations of manganese and arsenic in two mineral water samples were slightly higher than the standard international recommended limits. One mineral water sample had a fluoride concentration higher than the standard limits, whereas manganese was not detected in nine drinking and mineral water samples. Most of the selected minerals found in the tap water samples were below the international standard limits, except for iron and manganese. The concentrations of iron and manganese in the tap water samples were higher than the standard limits, which were obtained from one and three of the studied locations, respectively. The potable water obtained from various manufacturers and locations in Peninsular Malaysia is safe for consumption, as the minerals concentrations were below the standard limits prescribed by the Malaysian Food Regulations of 1985. The data obtained may also provide important information related to daily intake of these minerals from drinking water.


Sensors ◽  
2020 ◽  
Vol 20 (24) ◽  
pp. 7324
Author(s):  
Paulo M Zagalo ◽  
Paulo A Ribeiro ◽  
Maria Raposo

Hormones have a harmful impact on the environment and their detection in water bodies is an urgent matter. In this work, we present and analyze a sensor device able to detect traces of the synthetic hormone 17α-ethinylestradiol (EE2) below 10−9 M in media of different complexities, namely, ultrapure, mineral and tap waters. This device consists of solid supports with interdigitated electrodes without and with a polyethylenimine (PEI) and poly (sodium 4-styrenesulfonate) (PSS) layer-by-layer film deposited on it. Device response was evaluated through capacitance, loss tangent and electric modulus spectra and the data were analyzed by principal component analysis method. While the three types of spectra were demonstrated to be able to clearly discriminate the different media, loss tangent spectra allow for the detection of EE2 concentration, with a sensitivity of −0.072 ± 0.009 and −0.44 ± 0.03 per decade of concentration, for mineral and tap water, respectively. Detection limits values were found to be lower than the ones present in the literature and presenting values of 8.6 fM (2.6 pg/L) and of 7.5 fM (22.2 pg/L) for tap and mineral waters, respectively. Moreover, the obtained response values follow the same behavior with EE2 concentration in any medium, meaning that loss tangent spectra allow the quantification of EE2 concentration in aqueous complex matrices.


2013 ◽  
Vol 2013 ◽  
pp. 1-5 ◽  
Author(s):  
Ahmad Reza Bahrami ◽  
Ebrahim Rahimi ◽  
Hajieh Ghasemian Safaei

Helicobacter pyloriinfection in human is one of the most common infections worldwide. However, the origin and transmission of this bacterium has not been clearly explained. One of the suggested theories is transmission via water. This study was conducted to determine the prevalence rate ofH. pyloriin tap water, dental units' water, and bottled mineral water in Iran. In the present study, totally 200 water samples were collected in Isfahan province and tested forH. pyloriby cultural method and polymerase chain reaction (PCR) by the detection of theureC (glmM)gene. Using cultural method totally 5 cultures were positive. Two out of 50 tap water samples (4%), 2 out of 35 dental units' water (5.8%) samples, and 1 out of 40 (2.5% ) from water cooler in public places were found to be contaminated withH. pylori.H. pylori ureCgene was detected in 14 (7%) of water samples including 5 tap water (10%), 4 dental units' water (11.4%), 1 refrigerated water with filtration, and 4 (10%) water cooler in public places samples. This may be due to the coccoid form of bacteria which is detected by PCR method.


2008 ◽  
Vol 51 (5) ◽  
pp. 1049-1055 ◽  
Author(s):  
Denise de Oliveira Scoaris ◽  
Fernando Cezar Bizerra ◽  
Sueli Fumie Yamada-Ogatta ◽  
Benício Alves de Abreu Filho ◽  
Tânia Ueda-Nakamura ◽  
...  

The aim of this work was to study the occurrence of Aeromonas sp in the bottled mineral water, well water and tap water from the municipal supplies. Positive samples were found for Aeromonas spp. 12.7% from the mineral water, 8.3% from the artesian water and 6.5% from the tap water. The recovery of Aeromonas spp. was significantly higher in the bottled mineral and artesian water than in the tap water from municipal supplies. The occurrence of the Aeromonas spp. did not correlate significantly with the contamination indicator bacteria (i.e. total coliforms) in the artesian water samples. However, a significant correlation was found between Aeromonas spp. and total coliforms in the both mineral water and tap water samples. The presence or absence of a correlation between the indicator bacteria and Aeromonas could reflect the occasional appearance of the pathogen in the drinking water and the different rates of survival and recovery of these agents compared with those fecal indicators. The finding that 41.6, 14.8 and 9.0 % of the artesian water, bottled mineral water and tap water, respectively, sampled in the current study failed to meet the Brazilian standard for total coliforms in the drinking water should therefore be of concern.


Water ◽  
2019 ◽  
Vol 12 (1) ◽  
pp. 97
Author(s):  
Maria Karpińska ◽  
Jacek Kapała ◽  
Agnieszka Raciborska ◽  
Stanisław Mnich

Hydrotherapy is a natural treatment and health protection method. Treatments using natural mineral water are gaining popularity as an alternative to pharmacotherapy or as additional support to pharmacotherapy in many types of diseases. The aim of the study was to determine the effective dose obtained as a result of baths and inhalations using popular mineral water samples. A total of 18 commercially available water samples used in hydrotherapy were tested for their radioactive isotope content. The following isotopes were found: 40K, 208Tl, 212Bi, 212Pb, 214Bi, 214Pb, 226Ra, 228Ac, 234Th. Effective doses received by patients during inhalation and bathing using the tested mineral water samples were determined. The collected sample activity was measured using gamma spectrometry. The effective doses received by patients from a series of inhalation treatments ranged from 170.4 to 22.9 µSv. Infants receive the highest effective dose as a result of inhalation of mineral water. The doses received by patients as a result of bathing in the studied mineral water samples were in the range of from 0.04 to 1.1 µSv and were comparable with doses from ordinary baths in tap water (0.06 µSv). The determined doses are very low; thus, they are unlikely to cause noticeable biological effects.


2011 ◽  
Vol 11 (5) ◽  
pp. 642-646 ◽  
Author(s):  
Hassan Momtaz ◽  
Ebrahim Rahimi ◽  
Saadat Moshkelani

The purpose of this study was to consider the use of a simple polymerase chain reaction (PCR) as an accurate, safe and rapid method to detect Pseudomonas aeruginosa in tap-water and bottled mineral water from Isfahan province, Iran. A total of 224 tap-water and bottled mineral water samples were taken over six months, from July to December 2010. DNA (deoxyribonucleic acid) was extracted from water samples after filtration and culture and PCR was performed by primers derived from the ETA (Exotoxin A) operon gene sequence of the P. aeruginosa. Out of all the samples, 13.8% and 1.97% were positive by this molecular method for tap-water and bottled mineral water, respectively. The results show that PCR assay could become a valuable diagnostic or screening test for water quality.


Author(s):  
Ahmad Z Al-Herrawy ◽  
Mohamed A Marouf ◽  
Mahmoud A. Gad

Genus Acanthamoeba causes 3 clinical syndromes amebic keratitis, granulomatous amebic encephalitis and disseminated granulomatous amebic disease (eg, sinus, skin and pulmonary infections). A total of 144 tap water samples were collected from Giza governorate, Egypt. Samples were processed for detection of Acanthamoeba species using non-nutrient agar (NNA) and were incubated at 30oC. The isolates of Acanthamoeba were identified to species level based on the morphologic criteria. Molecular characterization of the Acanthamoeba isolates to genus level was performed by using PCR. The obtained results showed that the highest occurrence percentage of Acanthamoeba species in water samples was observed in summer season (38.9%), then it decreased to be 30.6% in spring and 25% in each of autumn and winter. PCR analysis showed that 100% of 43 Acanthamoeba morphologically positive samples were positive by genus specific primer. In the present study eight species of Acanthamoeba can be morphologically recognized namely Acanthamoeba triangularis, Acanthamoeba echinulata, Acanthamoeba astronyxis, Acanthamoeba comandoni, Acanthamoeba griffini, Acanthamoeba culbertsoni, Acanthamoeba quina and Acanthamoeba lenticulata. In conclusion, the most common Acanthamoeba species in tap water was Acanthamoeba comandoni


Author(s):  
V.V. Lapenko ◽  
L.N. Bikbulatova ◽  
E.M. Ternikova

Water is very important for humans, as it is a solvent for metabolic products. Moreover, it is necessary for metabolism, biochemical and transport processes. The elemental status in persons depends on the geochemical environment and consumption of bioelements with food and water. The aim of the paper is to conduct a comprehensive assessment of chemical composition of drinking tap water in Khanty-Mansiysk and Salekhard. Materials and Methods. The chemical composition of 100 samples of drinking tap water was analyzed by atomic absorption spectrometry, spectrophotometry and capillary electrophoresis. All in all, there were 50 samples from Khanty-Mansiysk and 50 samples from Salekhard. The results were compared with Sanitary Rules and Norms 2.1.4.1074-01. Results. Drinking tap water in Salekhard contains a significantly higher concentration of iron, which is much above the maximum allowable concentration, if compared to water samples in Khanty-Mansiysk (p=0.03). In the cities under consideration, the water undergoes high-quality reagent-free treatment. However, the deterioration of the water supply networks in Salekhard is 3 times as high as in Khanty-Mansiysk. Calcium and magnesium concentration in water samples from Khanty-Mansiysk is 5.6 and 3.9 times lower than the MAC; in water samples from Salekhard calcium concentration is 6.3 (p=0.008) and magnesium concentration 4.6 (p<0.001) times lower than the MAC. Conclusion. The consumption of ultra-fresh drinking water leads to low intake of bioelements, which are a part of enzymes contributing to the human antioxidant defense and can result in manifestation of cardiovascular diseases. This is especially true for Salekhard with very soft drinking water and high iron concentration, which excess can exhibit prooxidant properties. Keywords: tap water, bioelements, northern region, antioxidants. Вода является важнейшим соединением для человека: необходима в качестве растворителя продуктов метаболизма и протекания обменных, биохимических и транспортных процессов. Элементный статус организма человека зависит от геохимического окружения и поступления биоэлементов с пищей и водой. Цель. Провести комплексную оценку химического состава водопроводной воды городов Ханты-Мансийск и Салехард. Материалы и методы. Методами атомно-абсорбционной спектрометрии, спектрофотометрии и капиллярного электрофореза проанализирован химический состав 100 проб водопроводной воды: по 50 из Ханты-Мансийска и Салехарда. Результаты сравнивали с СанПиН 2.1.4.1074-01. Результаты. В водопроводной воде Салехарда установлена превышающая ПДК и достоверно более высокая концентрация железа сравнительно с водой Ханты-Мансийска (р=0,03). При условии качественной безреагентной водоподготовки в изучаемых городах это обусловлено изношенностью водопроводных сетей в Салехарде, более чем в 3 раза превышающей этот показатель в Ханты-Мансийске. Концентрация кальция и магния в воде Ханты-Мансийска в 5,6 и 3,9 раза ниже ПДК; в воде Салехарда – в 6,3 (р=0,008) и 4,6 (р<0,001) раза ниже ПДК соответственно. Заключение. Употребление ультрапресной питьевой воды на фоне очень малого поступления с водой биоэлементов, входящих в состав ферментов антиоксидантной защиты организма человека, может привести к манифестации кардиоваскулярных заболеваний. Это особенно актуально для г. Салехарда с очень мягкой питьевой водой с повышенным содержанием железа, избыток которого может проявлять прооксидантные свойства. Ключевые слова: водопроводная вода, биоэлементы, северный регион, антиоксиданты.


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