INDUCED BREAST CANCER MCF-7 CELLS APOPTOSIS FROM EXTRACT COMBINATION OF JENGKOL PODS (Archidendron jiringa) AND PETAI CINA LEAVES (Leucaena leucocephala)

The jengkol pod exocarp and petai cina leaves potentially as breast anticancer due to its highly toxic. The activity of cytotoxicity to the MCF-7 cells by the combination of jengkol pod exocarp and petai cina leaves is included in the potential category. The research aimed to determine the influence of the combination of jengkol pod exocarp and petai cina leaves on induction of the MCF-7 breast cancer apoptosis. Induction cell apoptosis of MCF-7 from a combination of extracts by using a double staining method. The cytotoxicity test from the extract combination of jengkol pod exocarp and petai cina leaves was determined by the MTT method. The extracts were made by comparing the mass of jengkol pod exocarp and petai cina leaves with comparisons of 5:1, 7:1, and 9:1. The IC50 values of the combination of jengkol pod exocarp and petai cina leave the ratio of 5:1, 7:1, and 9:1 were 11.7; 7.5; and 1.9 ppm, respectively. Apoptosis activity of the extract combination of the double staining test results showed MCF-7 cells experiencing orange and bright green fluorescence. The cellular form becomes wrinkled from the initial condition of the cell. Based on the results of the study showed a combination of jengkol pod exocarp and petai cina leaves could induce the MCF-7 breast cancer apoptosis cell.

PHYTOCHEMICAL AND PHARMACOLOGICAL ASPECTS OF ANTHRAQUINONES OF RHEUM AUSTRALE D. DON

Anthraquinones are phenolic compounds known best for their laxative activity. Rheum australe D. Don (Indian rhubarb, Polygonaceae) is a prominent medicinal plant with anthraquinones as the bioactive compounds, among a few others. The online literature search was carried out to collect data on the phytochemistry and pharmacological activity of anthraquinones, particularly those isolated from R. australe. Anthraquinones are commonly found in the genera of Rheum, Senna, Aloe, Frangula, and Rubia, which can be quantitatively and qualitatively determined using both conventional and advanced analytical methods. The anthraquinones of R. australe were found in free and glycosidic forms, which were best extracted by the microwave-assisted extraction method. Various chromatographic techniques were commonly conducted to isolate the pure compounds. In addition to its laxative activity, anthraquinones of R. australe also showed potential antibacterial, cytotoxic, and antioxidant properties.

A SURVEY OF PHARMACY STAFF KNOWLEDGE RELATED TO ANTIBIOTICS IN SIDOARJO, INDONESIA

Antibiotics resistance still remains the problem in treating infection disease both in developing and developed country. One of the factor contribute to the increase of antibiotics resistance is irrational antibiotics use without prescriptions. There is still lack of information about factors related to the antibiotics dispense by pharmacy staff. This study was aimed to observe pharmacy staff knowledge about the pharmacology and the regulation of antibiotics. A cross sectional online survey using questionnaire was conducted to pharmacy staff who work for service in Sidoarjo from February to April 2020. The knowledge of antibiotics was classified into lack, enough, and good knowledge. A total of 233 respondents from 56 pharmacy recruited in this study. Of them, 17,17% were pharmacist; 10,73% were pharmacy technician; 52,36% were pharmacy assistant and 19,74% non pharmacy worker. Overall the majority of respondents had a good knowledge about antibiotics (48,93%). However, most of them did not know that only pharmacist who had responsible to dispense antibiotics based on prescriptions (58,37%), fail to describe antibiotics indications (46,35%) and had poor knowledge about the minimum length of antibiotics course (58,37%). Educating and upskilling pharmacy personel about antibiotics will minimize the irrational use of antibiotics which lead to the increase of antibiotics resistance caused by the lack information received by patients.

ANTIDIABETIC ACTIVITY FROM TABLETS EFFERVESCENT EXTRACT OF MIMOSA PUDICA LINN LEAVES

The community has trusted mimosa pudica linn as an alternative antidiabetic treatment. Compounds that have antidiabetic activity in Mimosa pudica linn are flavonoids, alkaloids, and tannins. The selection of effervescent tablet dosage formulations is practical for diabetics with the addition of stevia leaf sweeteners. This study aimed to determine the antidiabetic activity of effervescent tablets of Mimosa pudica linn leaves extract as a natural antidiabetic. The research method begins with the extraction of plants using the maceration method of 70% ethanol; the tablet formulation uses dry granulation. Antidiabetic activity test using 28 male mice (Mus musculus) Swiss strain divided into four groups, namely group I (negative control): Na-CMC 0.5%, group II: effervescent tablets of Mimosa pudica Linn leaves Extract dose 600 mg/kgBB, group III (positive control): metformin 65 mg/kgBB, group IV (normal control): feed and drink. Each group, except normal controls, was induced by DM using alloxan 150 mg/kg body weight. Data on decreased blood glucose levels were analyzed using the Kruskal-Wallis statistical test the study results show that effervescent tablets meet the parameters of disperse time test, pH test, size uniformity test, weight uniformity test, hardness test, moisture test, and friability test. This study concludes is that there is a significant difference between the negative, positive, normal, and treatment control groups, which means that the administration of effervescent tablets of 70% ethanol extract of Mimosa pudica Linn leaves at a dose of 600 mg/kgBB can reduce blood sugar levels in mice (Mus musculus).

FORMULATION AND PHYSICAL PROPERTIES OF CANDLENUT OIL GEL POMADE (Aleurites Mollocana Wild)

The use of pomade for a long time will cause hair damage. Pomade with preferred ingredients, because it is very good for beauty and hair health. Liquid oil is one of the basic ingredients used in making pomade. Candlenut oil can be used as liquid oil for the pomade base. This article examines the formulation and physical properties of pomade from candlenut oil. There are four formulas that have been made with different F0 candlenut oils without candlenut oil, F1 with 10% candlenut oil, F2 with 20% candlenut oil, F3 with 30% candlenut oil. The difference in concentration is indicated to affect the physical differences of the candlenut oil pomade. Physical properties of Candlenut Oil Pomade gel include organoleptic, homogeneity, scattering test, sticky test. The results showed that all formulas showed homogeneous gel preparations. Formula 3, with the highest concentration of candlenut oil concentration of 30%, shows a greater dispersion than a formula with other substances reaching 6.5 cm and meets SNI standards, which will increase the adhesion, which does not meet the standard.

DETERMINATION OF FLAVONOID LEVELS OF AlCl3 METHODE IN THE EXTRACT OF METANOL FLOWERS (Clitoria ternatea L.)

Telang flowers is one of the medicinal plants that can grow well in Indonesia, but have not been widely used. Previous research stated that methanol extract of telang flowers had secondary metabolite compounds such as tannin, saponin, flavonoid and alkaloid. Flavonoid are natural compounds that have the potential as antioxidants that can capture free radicals that play a role in the emergence of degenerative diseases throught the mechanisme of damage to the body’s immune system, lipid oxidation and protein.The purpose of this study was to determine whether telang flowers content was determined whether by the AlCl3 method and measured by using UV-Vis Spektrophotometry from the methanol extract of telang flowers.The results of this study indicate that the methanol extract of telang flowers contains flavonoid with red color. The level of flavonoids intended is 4.65%.

COMPARISON BETWEEN PHYSICAL AND NON-PHYSICAL WORK ENVIRONMENT IN PBF X YOGYAKARTA

The convenience of working for employees is one of the keys to success for the company. If employees feel comfortable, their work productivity will increase and have a positive impact on the company. One factor that can provide comfort to employees is the work environment. The work environment is dividing into a physical work environment and a non-physical work environment. This study aims to determine the comparison of physical and non-physical work environments in PBF X in Yogyakarta. The method used is descriptive-analytic, with a cross-sectional approach and using saturated samples. Primary data were obtained by distributing questionnaires to all 25 PBF X employees. Data were processed using Microsoft Excel and presented in tables, diagrams, and descriptive (narrative) forms. The results obtained show a comparison of the mean value of the non-physical work environment is 3.0, and the mean value of the physical work environment is 2.8. The highest mean value in the non-physical work environment is about overtime salaries, while in the physical work environment, selecting paint colors at workplaces appropriate. The conclusion obtained that the non-physical work environment is more influential on respondents doing work in PBF X than the physical work environment. The company or PBF X needs to evaluate to create a comfortable physical work environment for employees so that the working atmosphere can increase company productivity

COMPLIANCE TO MEDICATION IN HYPERTENSIVE PATIENTS OF CHRONIC DISEASE MANAGEMENT CONSULTING PHARMACIST AT PUBLIC HEALTH CENTER OF SEMARANG CITY

One of the pharmaceutical services that can be done by pharmacists is counseling. Counseling lead to increase the obedience to taking medication in reduce hypertension. The objective of this research is to evaluate the obedience to drug therapy of hypertensive patient which already get counseling by pharmacists in Public Health Center in Semarang. This research was carried out by cross sectional design. The research was conducted towards 40 patients who were willing to take part in the research by filling out inform consent. “Pill count” method was used to evaluate the obedience level by counting the remaining drug for 1 month starting from the beginning until the end of July 2019. These results will be analyzed by chi-square test to determine the relationship between the characteristics and the obedience level. Wilcoxon test was done to determine the differences in groups before and after given counseling. The results showed that there was a significant decrease in systolic and diastolik pressure (p = 0,000). Chi-square test results showed that counseling given by pharmacists had no significance effect related to the obedience to drug therapy of hypertensive prolanis patient with a statistic of p> 0.05.

PARENT'S KNOWLEDGE REGARDING INFLUENZA DISEASE AND VACCINE IN INDONESIA

Influenza vaccine is a vaccine that is not included in the national immunization program. An approach to support decision making for the introduction of influenza vaccine into national immunization programs by conducting a survey knowledge of parents in Indonesia that influences decision making for vaccinations. The purpose of this study was to determine the level of parental knowledge about influenza and influenza vaccines and to know the factors that influence. The research design used is observasional with a multi-center cross-sectional approach. The research instrument used a questionnaire to determine sociodemographic characteristics and level of knowledge. Data was collected through a survey of 500 parents respondents in 5 provinces in Indonesia, namely in West Java, DIY, Lampung, Central Sulawesi and Central Kalimantan using convenience sampling techniques. Data were analyzed using the Mann Whitney test. Resul of the study showed the average knowledge of respondents related to influenza disease and vaccine was 78.55±12.43. Only 145 (29%) parents know that influenza vaccines are not included in Indonesia's national immunization program. There was a significant difference in the value of knowledge between groups of characteristics in the experience of hearing influenza vaccine (p = 0,000) and the source of disease information (p = 0.045). It is recommended for government and health workers to carry out educational programs related to influenza and influenza vaccines to achieve equitable distribution of information and increase public knowledge.

EFFECT OF PHARMACEUTICAL CARE ON LEVEL OF SATISFACTION AND KNOWLEDGE IN HYPERTENSIVE PATIENTS AT THE RSUD DR. SOEKARDJO CITY OF TASIKMALAYA

High blood pressure is described as an increase in blood vessel pressure when the heart pumps against the walls of the blood vessels. Satisfaction is the feeling of satisfaction received by the patient/person experiencing the treatment. This study aims to measure the level of adherence, satisfaction, and knowledge of drug use before and after counselling. This research was conducted experimentally with primary data collection in the form of information from patients prospectively from February to April 2019. The instruments used were the compliance questionnaire (MMAS-8), satisfaction, and knowledge. Respondents involved in this study were 50 people. The testing group was divided into two, namely the group that was given treatment and the group that was not given treatment, where the treatment group was given counselling by the pharmacist. The results of statistical testing showed a value (p <0.05) on the level of compliance, satisfaction, and knowledge. The test results showed a significant value (P <0.05) between compliance with knowledge and satisfaction. Meanwhile, there is no significant result between satisfaction and knowledge. It can be concluded that there is a correlation between the level of knowledge with compliance, compliance with satisfaction and there are differences in the level of compliance, satisfaction, and knowledge before and after being given pharmaceutical/counselling care.