Enrichment of Broiler Chickens’ Meat with Dietary Linseed Oil and Lysine Mixtures: Influence on Nutritional Value, Carcass Characteristics and Oxidative Stress Biomarkers

This study aimed to evaluate the effect of four combinations of dietary linseed oil and lysine mixtures on performance, fatty and amino acid profiles, oxidative stress biomarkers, cell energy and meat quality parameters of broiler chickens. One hundred and sixty broiler chicks were allocated into four groups. Birds of groups 1–4 were fed diets containing optimum lysine and 2% of linseed oil, optimum lysine and 4% of linseed oil, high lysine and 2% of linseed oil, and high lysine and 4% of linseed oil, respectively, for a period of 35 days. High linseed oil or lysine levels did not affect the performance of the tested birds, but the high level of dietary linseed oil decreased the concentrations of muscles’ saturated fatty acids (SFA). The highest values of ω-3 polyunsaturated fatty (ω-3 PUFA) and arachidonic acids with lowest levels of monounsaturated fatty (MUFA) were detected in the muscles of birds fed diets containing high linseed oils and/or lysine levels. High linseed oil or lysine levels provided the best essential amino acid profile and improved antioxidant components as well as cell energy, and tenderness and redness of the meat. Conclusively, high dietary lysine and linseed oil combinations improved the nutritional value, antioxidant status and cell energy of broiler chickens’ meat.

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Antioxidative Efficacy of a Pistacia Lentiscus Supplement and Its Effect on the Plasma Amino Acid Profile in Inflammatory Bowel Disease: A Randomised, Double-Blind, Placebo-Controlled Trial

Nutrients ◽

10.3390/nu10111779 ◽

2018 ◽

Vol 10 (11) ◽

pp. 1779 ◽

Cited By ~ 8

Author(s):

Efstathia Papada ◽

Alastair Forbes ◽

Charalampia Amerikanou ◽

Ljilja Torović ◽

Nick Kalogeropoulos ◽

...

Keyword(s):

Oxidative Stress ◽

Inflammatory Bowel Disease ◽

Amino Acid ◽

Density Lipoprotein ◽

Pistacia Lentiscus ◽

Oxidative Stress Biomarkers ◽

Double Blind ◽

Double Blind Placebo ◽

Stress Biomarkers ◽

Inflammatory Bowel

Oxidative stress is present in patients with Inflammatory Bowel Disease (IBD), and natural supplements with antioxidant properties have been investigated as a non-pharmacological approach. The objective of the present study was to assess the effects of a natural Pistacia lentiscus (PL) supplement on oxidative stress biomarkers and to characterise the plasma-free amino acid (AA) profiles of patients with active IBD (Crohn’s disease (CD) N = 40, ulcerative colitis (UC) N = 20). The activity was determined according to 5 ≤ Harvey Bradshaw Index ≤ 16 or 2 ≤ Partial Mayo Score ≤ 6. This is a randomised, double-blind, placebo-controlled clinical trial. IBD patients (N = 60) were randomly allocated to PL (2.8 g/day) or to placebo for 3 months being under no treatment (N = 21) or under stable medical treatment (mesalamine N = 24, azathioprine N = 14, and corticosteroids N = 23) that was either single medication (N = 22) or combined medication (N = 17). Plasma oxidised, low-density lipoprotein (oxLDL), total serum oxidisability, and serum uric acid were evaluated at baseline and follow-up. OxLDL/LDL and oxLDL/High-Density Lipoprotein (HDL) ratios were calculated. The plasma-free AA profile was determined by applying a gas chromatography/mass spectrometry analysis. oxLDL (p = 0.031), oxLDL/HDL (p = 0.020), and oxLDL/LDL (p = 0.005) decreased significantly in the intervention group. The mean change differed significantly in CD between groups for oxLDL/LDL (p = 0.01), and, in the total sample, both oxLDL/LDL (p = 0.015) and oxLDL/HDL (p = 0.044) differed significantly. Several changes were reported in AA levels. PL ameliorated a decrease in plasma-free AAs seen in patients with UC taking placebo. In conclusion, this intervention resulted in favourable changes in oxidative stress biomarkers in active IBD.

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OXIDATIVE STRESS BIOMARKERS IN EXPIRATED AIR OF HEALTHY HUMANS AND PROMISING TECHNIQUES OF THEIR ANALYSIS DURING SPACE FLIGHT

Aerospace and Environmental Medicine ◽

10.21687/0233-528x-2020-54-3-5-21 ◽

2020 ◽

pp. 5-21

Author(s):

L.N. Mukhamedieva ◽

◽

D.S. Tsarkov ◽

D.S. Ozerov ◽

G.Yu. Grigoriev ◽

...

Keyword(s):

Oxidative Stress ◽

Space Flight ◽

Oxidative Stress Biomarkers ◽

Stress Biomarkers ◽

Healthy Humans

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Association between Vitamin D Status, Oxidative Stress Biomarkers and Viral Load in Human Immunodeficiency Virus Type 1 Infection

Current HIV Research ◽

10.2174/1570162x15666171005170227 ◽

2017 ◽

Vol 15 (5) ◽

Cited By ~ 4

Author(s):

Tamires Flauzino ◽

Andrea N.C. Simao ◽

Elaine R.D. de Almeida ◽

Helena K. Morimoto ◽

Sayonara R. Oliveira ◽

...

Keyword(s):

Oxidative Stress ◽

Vitamin D ◽

Human Immunodeficiency Virus ◽

Viral Load ◽

Human Immunodeficiency Virus Type ◽

Vitamin D Status ◽

Oxidative Stress Biomarkers ◽

Stress Biomarkers ◽

Immunodeficiency Virus

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Oxidative Stress Biomarkers in Urine of Metal Carpentry Workers Can Be Diagnostic for Occupational Exposure to Low Level of Welding Fumes from Associated Metals

Cancers ◽

10.3390/cancers13133167 ◽

2021 ◽

Vol 13 (13) ◽

pp. 3167

Author(s):

Flavia Buonaurio ◽

Maria Luisa Astolfi ◽

Daniela Pigini ◽

Giovanna Tranfo ◽

Silvia Canepari ◽

...

Keyword(s):

Oxidative Stress ◽

Occupational Exposure ◽

Oxidation Products ◽

Control Group ◽

Welding Fumes ◽

Oxidative Stress Biomarkers ◽

Limit Values ◽

Stress Biomarkers ◽

Specificity And Sensitivity ◽

The Impact

Urinary concentrations of 16 different exposure biomarkers to metals were determined at the beginning and at the end of a working shift on a group of workers in the metal carpentry industry. Five different oxidative stress biomarkers were also measured, such as the oxidation products of RNA and DNA metabolized and excreted in the urine. The results of workers exposed to metals were compared to those of a control group. The metal concentrations found in these workers were well below the occupational exposure limit values and exceeded the mean concentrations of the same metals in the urine of the control group by a factor of four at maximum. Barium (Ba), mercury (Hg), lead (Pb) and strontium (Sr) were correlated with the RNA oxidative stress biomarker, 8-oxo-7, 8-dihydroguanosine (8-oxoGuo), which was found able to discriminate exposed workers from controls with a high level of specificity and sensitivity. The power of this early diagnostic technique was assessed by means of the ROC curve. Ba, rubidium (Rb), Sr, tellurium (Te), and vanadium (V) were correlated with the level of the protein oxidation biomarker 3-Nitrotyrosine (3-NO2Tyr), and Ba, beryllium (Be), copper (Cu), and Rb with 5-methylcytidine (5-MeCyt), an epigenetic marker of RNA damage. These effect biomarkers can help in identifying those workers that can be defined as “occupationally exposed” even at low exposure levels, and they can provide information about the impact that such doses have on their health.

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Antioxidants, lysosomes and elements status during the life cycle of sea trout Salmo trutta m. trutta L.

Scientific Reports ◽

10.1038/s41598-021-85127-3 ◽

2021 ◽

Vol 11 (1) ◽

Author(s):

Natalia Kurhaluk ◽

Halyna Tkachenko

Keyword(s):

Oxidative Stress ◽

Lipid Peroxidation ◽

Muscle Tissue ◽

Salmo Trutta ◽

Protein Carbonyl ◽

Oxidative Modification ◽

Oxidative Stress Biomarkers ◽

Development Stages ◽

Stress Biomarkers ◽

Element Contents

AbstractThe aim of our study was to elucidate the effects of both development stages (parr, smolt, adult, spawner), and kelt as a survival form and sex (male, female) on the functional stability of the lysosomal complex, biomarkers of oxidative stress, and element contents in the muscle tissue of the sea trout (Salmo trutta m. trutta L.) sampled in the Pomerania region (northern Poland). We have evaluated the maximal activities of lysosomal enzymes (alanyl aminopeptidase, leucyl aminopeptidase, β-N-acetylglucosaminidase, and acid phosphatase), lipid peroxidation level, and protein carbonyl derivatives as indices of muscle tissue degradation. The relationship between lysosomal activity and oxidative stress biomarkers estimated by the lipid peroxidation level and protein carbonyl derivatives was also assessed, as well as the relationships between element levels and oxidative stress biomarkers. Trends of the main effects (i.e., the development stages and sex alone, the interaction of the sex and development stage simultaneously) on oxidative stress biomarkers, lysosomal functioning, and element contents in the muscle tissue were evaluated. The study has shown sex-related relationships between the pro- and antioxidant balance and the tissue type in the adult stage as well as modifications in the lysosomal functioning induced by long-term environmental stress associated with changing the habitats from freshwater to seawater and intense migrations. The highest level of toxic products generated in oxidative reactions and oxidative modification of proteins was noted in both the spawner stage and the kelt form. The holistic model of analysis of all parameters of antioxidant defense in all development stages and sex demonstrated the following dependencies for the level of lipid peroxidation, oxidative modification of proteins, lysosomal activities, and element contents: TBARS > OMP KD > OMP AD > TAC, AcP > NAG > LAP > AAP and Cu > Fe > Ca > Mn > Zn > Mg, respectively.

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