scholarly journals Effect of Physical and Enzymatic Pre-Treatment on the Nutritional and Functional Properties of Fermented Beverages Enriched with Cricket Proteins

Foods ◽  
2021 ◽  
Vol 10 (10) ◽  
pp. 2259
Author(s):  
Chaima Dridi ◽  
Mathieu Millette ◽  
Blanca Aguilar ◽  
Johanne Manus ◽  
Stephane Salmieri ◽  
...  
Keyword(s):  
Enzymatic Hydrolysis ◽  
Protein Solubility ◽  
Protein Digestibility ◽  
Surface Hydrophobicity ◽  
Combined Treatments ◽  
Γ Irradiation ◽  
Fermented Beverages ◽  
Fermented Beverage ◽  
Pre Treatment

The aim of this study was to evaluate the effects of γ-irradiation (IR), ultrasound (US), and combined treatments of ultrasound followed by γ-irradiation (US-IR), ultrasound followed by enzymatic hydrolysis with and without centrifugation (US-E and US-EWC, respectively), and ultrasound followed by γ-irradiation and enzymatic hydrolysis (US-IRE), on the digestibility and the nutritional value of fermented beverages containing probiotics. Results showed that US (20 min), IR (3 kGy) and US-IR (tUS = 20 min, dose = 3 kGy) treatments raised protein solubility from 11.5 to 21.5, 24.3 and 29.9%, respectively. According to our results, these treatments were accompanied by the increased amount of total sulfhydryl groups, surface hydrophobicity and changes to the secondary structure of the proteins measured by Fourier-transform infrared spectroscopy (FTIR). Fermented probiotic beverages, non-enriched (C) and enriched with untreated (Cr) or treated cricket protein with combined treatments were also evaluated for their in vitro protein digestibility. Results showed that the soluble fraction of US-IRE fermented beverage had the highest digestibility (94%) as compared to the whole fermented tested beverages. The peptides profile demonstrated that US-IRE had a low proportion of high molecular weight (MW) peptides (0.7%) and the highest proportion of low MW peptides by over 80% as compared to the other treatments.

Effects of Fermentation on Nutritional and Functional Properties of Soybean, Maize, and Germinated Sorghum Composite Flour

2012 ◽  
Vol 8 (1) ◽  
Author(s):  
Nicole Murekatete ◽  
Yufei Hua ◽  
Xiangzhen Kong ◽  
Caimeng Zhang
Keyword(s):  
Protein Solubility ◽  
Titratable Acidity ◽  
Protein Digestibility ◽  
Composite Flour ◽  
In Vitro Protein Digestibility ◽  
Food Ingredient ◽  
Fermentation Time ◽  
Nutrient Density ◽  
Vitro Protein

Sorghum germination resulted in a substantial tannin loss (95.7 %). Proximate composition, titratable acidity, pasting properties, in vitro protein digestibility, and protein solubility were studied post fermentation (Saccharomyces Cerevisiae) of the blended soybean, maize, and germinated sorghum flours. The pH progressively decreased with fermentation time, while titratable acidity increased from 0.029 to 0.118 ml/ml. Crude protein content increased with fermentation (251.7-274.8 mg/g) as a result of a shift in the dry matter composition. In-vitro protein digestibility markedly increased (12 %) as a result of fermentation. Protein solubility curves were above 30% of which highest for both fermented (12 and 24 hours) and unfermented composite flours were at pH 12 (51.77-77.64%) and lowest at pH 4 (30.31-35.98%). SDS-PAGE showed that protein hydrolysis occurred during fermentation over 12 and 24 hours. Unfermented composite flour was potentially stable as food ingredient due to its pasting stability, but the fermented flour low viscosity potential was preferred in this study as more flour will be used during porridge making, hence giving a food with a high nutrient density.

Antinutritional factors, ruminal degradation and in vitro protein digestibility of gamma irradiated full-fat soybean

2009 ◽  
Vol 2009 ◽  
pp. 184-184
Author(s):  
M Taghinejad Roudbaneh ◽  
A Nikkhah ◽  
A A Sadeghi ◽  
G Raisali ◽  
M Chamani
Keyword(s):  
Gamma Irradiation ◽  
Dairy Cows ◽  
Nutritional Value ◽  
Protein Digestibility ◽  
High Energy ◽  
Antinutritional Factors ◽  
Γ Irradiation ◽  
Rumen Microbes ◽  
Gamma Irradiated

Whole soybean is used as a high energy-protein supplement for dairy cows, but contains various antinutritional factors and the protein is highly degradable by rumen microbes. Roasting and extrusion are very popular ways of feeding soybeans to dairy cows but these processing methods may adversely affect the protein digestibility and lysine availability of the final product in the small intestine. Gamma irradiation has been recognized as a reliable and safe method for improving the nutritional value and inactivation or removal of certain antinutritional factors in foods and feeds (Siddhuraju et al., 2002). As far as we know, information about the effects of γ-irradiation on ruminal crude protein (CP) degradation of full-fat soybean in the literature is scarce. The aim of this study was to evaluate the effects of gamma irradiation (γ-irradiation) on nutritional value and antinutritional factors of soybean (SB) for ruminants.

scholarly journals Prediction of crude protein digestibility of animal by-product meals for dogs by the protein solubility in pepsin method

2014 ◽  
Vol 3 ◽  
Author(s):  
Iris M. Kawauchi ◽  
Nilva K. Sakomura ◽  
Cristiana F. F. Pontieri ◽  
Aline Rebelato ◽  
Thaila C. Putarov ◽  
...  
Keyword(s):  
Crude Protein ◽  
Pearson Correlation ◽  
Protein Solubility ◽  
Correlation Coefficients ◽  
Basal Diet ◽  
Protein Digestibility ◽  
Large Variability ◽  
The Relationship

AbstractAnimal by-product meals have large variability in crude protein (CP) content and digestibility. In vivo digestibility procedures are precise but laborious, and in vitro methods could be an alternative to evaluate and classify these ingredients. The present study reports prediction equations to estimate the CP digestibility of meat and bone meal (MBM) and poultry by-product meal (PM) using the protein solubility in pepsin method (PSP). Total tract CP digestibility of eight MBM and eight PM samples was determined in dogs by the substitution method. A basal diet was formulated for dog maintenance, and sixteen diets were produced by mixing 70 % of the basal diet and 30 % of each tested meal. Six dogs per diet were used to determine ingredient digestibility. In addition, PSP of the MBM and PM samples was determined using three pepsin concentrations: 0·02, 0·002 and 0·0002 %. The CP content of MBM and PM ranged from 39 to 46 % and 57 to 69 %, respectively, and their mean CP digestibility by dogs was 76 (2·4) and 85 (2·6) %, respectively. The pepsin concentration with higher Pearson correlation coefficients with the in vivo results were 0·0002 % for MBM (r 0·380; P = 0·008) and 0·02 % for PM (r 0·482; P = 0·005). The relationship between the in vivo and in vitro results was better explained by the following equations: CP digestibility of MBM = 61·7 + 0·2644 × PSP at 0·0002 % (P = 0·008; R2 0·126); and CP digestibility of PM = 54·1 + 0·3833 × PSP at 0·02 % (P = 0·005; R2 0·216). Although significant, the coefficients of determination were low, indicating that the models were weak and need to be used with caution.

Antifungal activity of combined treatments of active methylcellulose-based films containing encapsulated nanoemulsion of essential oils and γ–irradiation: in vitro and in situ evaluations

Cellulose ◽  
2018 ◽  
Vol 26 (2) ◽  
pp. 1335-1354 ◽  
Author(s):  
Farah Hossain ◽  
Peter Follett ◽  
Khanh Dang Vu ◽  
Stephane Salmieri ◽  
Carole Fraschini ◽  
...  
Keyword(s):  
Antifungal Activity ◽  
Essential Oils ◽  
Combined Treatments ◽  
Γ Irradiation

scholarly journals Peningkatan Mutu Jerami Padi, Dedak Padi dan Onggok dengan Fermentasi Fungi dan Yeast

2012 ◽  
Vol 12 (2) ◽  
pp. 24-32
Author(s):  
S.N.O. Suwandyastuti ◽  
Efka Aris Rimbawanto ◽  
Prayitno Prayitno
Keyword(s):  
Rice Straw ◽  
Rice Bran ◽  
Nutritive Value ◽  
Block Design ◽  
Protein Solubility ◽  
Protein Digestibility ◽  
Randomized Block Design ◽  
Pollution Problem ◽  
Cassava Waste

The quality improvement of rice straw, rice bran and cassava waste by fermentation of fungi and yeast ABSTRACT. Biomass of agricultural residues are highly potential as ruminant feedstuff. However, it is characterized by high content of indigestible fiber and low nutritive value, due to the strong hydrogen bonds in the lignocelluloses. Biological treatment by using microbes seems to be an alternative, because of the capability, with no pollution problem. An experiment has been conducted to seek for the fungi and yeast which capable to improve the quality of rice straw, rice bran and cassava waste. The trial was done by the technique of in sacco and in vitro, in a completely randomized block design. The variables measure were : dry matter and protein digestibility, protein solubility and nutrient composition of the fermentation product. Based on the all variables measure, the current study concluded that the microbes chosen were : monoculture of T. viride for rice straw, monoculture of A. niger for rice bran and biculture of A luchuensis and S. cereviseae for cassava waste.

Assessment of Protein Digestibility by In Vitro Enzymatic Hydrolysis with Simultaneous Dialysis

1982 ◽  
Vol 112 (9) ◽  
pp. 1718-1725 ◽  
Author(s):  
Sylvie F. Gauthier ◽  
Carol Vachon ◽  
John D. Jones ◽  
Laurent Savoie
Keyword(s):  
Enzymatic Hydrolysis ◽  
Protein Digestibility

INHIBITION OF THYROGLOBULIN BIOSYNTHESIS AND DEGRADATION BY EXCESS IODIDE. SYNERGISM WITH LITHIUM

1976 ◽  
Vol 81 (2) ◽  
pp. 495-506 ◽  
Author(s):  
A. Radvila ◽  
R. Roost ◽  
H. Bürgi ◽  
H. Kohler ◽  
H. Studer
Keyword(s):  
Protein Synthesis ◽  
Thyroid Gland ◽  
Inhibitory Action ◽  
Inhibit Protein Synthesis ◽  
Thyrotrophic Hormone ◽  
Thyroid Glands ◽  
Pre Treatment ◽  
Excess Iodide ◽  
Kidney Liver

ABSTRACT Lithium and excess iodide inhibit the release of thyroid hormone from preformed stores. We thus tested the hypothesis that this was due to an inhibition of thyroglobulin breakdown. Rats were pre-treated with propylthiouracil (PTU) for 3 weeks in order to deplete their thyroids of thyroglobulin. While the PTU was continued, lithium chloride (0.25 mEq./100 g weight) or potassium iodide (3 mg per rat) were injected every 12 h for 3 days. Thereafter the thyroglobulin content in thyroid gland homogenates was measured. PTU pre-treatment lowered the thyroglobulin content from 4.21 to 0.22 mg/100 mg gland. Lithium caused a marked re-accumulation of thyroglobulin to 0.60 mg/100 mg within 3 days. While iodide alone had only a borderline effect, it markedly potentiated the action of lithium and a combination of the two drugs increased the thyroglobulin content to 1.04 mg/100 mg. Thyroxine was injected into similarly pre-treated animals to suppress secretion of thyrotrophic hormone. This markedly inhibited the proteolysis of thyroglobulin and 1.3 mg/100 mg gland accumulated after 3 days. Excess iodide, given in addition to thyroxine, decreased the amount of thyroglobulin accumulated to 0.75 mg/100 mg gland. To study whether this could be explained by an inhibitory action of iodide on thyroglobulin biosynthesis, thyroid glands from animals treated with excess iodide were incubated in vitro in the presence of 0.2 mm iodide for 3 h. Iodide decreased the incorporation of radioactive leucine into total thyroidal protein and into thyroglobulin by 25 and 35 % respectively. Iodide did not inhibit protein synthesis in the kidney, liver or muscle tissue. Thus, large doses of iodide selectively inhibit thyroglobulin biosynthesis.

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