scholarly journals Latest Advances in Protein-Recovery Technologies from Agricultural Waste

Foods ◽  
2021 ◽  
Vol 10 (11) ◽  
pp. 2748
Author(s):  
Farhana Iylia Fatinee Mohd Yusree ◽  
Angela Paul Peter ◽  
Mohd Zuhair Mohd Nor ◽  
Pau Loke Show ◽  
Mohd Noriznan Mokhtar
Keyword(s):  
High Efficiency ◽  
Agricultural Waste ◽  
Cost Effective ◽  
Extraction Methods ◽  
Water Soluble ◽  
Membrane Extraction ◽  
Protein Recovery ◽  
Two Phase ◽  
Phase Partitioning ◽  
Extraction And Purification

In recent years, downstream bioprocessing industries are venturing into less tedious, simple, and high-efficiency separation by implementing advanced purification and extraction methods. This review discusses the separation of proteins, with the main focus on amylase as an enzyme from agricultural waste using conventional and advanced techniques of extraction and purification via a liquid biphasic system (LBS). In comparison to other methods, such as membrane extraction, precipitation, ultrasonication, and chromatography, the LBS stands out as an efficient, cost-effective, and adaptable developing method for protein recovery. The two-phase separation method can be water-soluble polymers, or polymer and salt, or alcohol and salt, which is a simpler and lower-cost method that can be used at a larger purification scale. The comparison of different approaches in LBS for amylase purification from agricultural waste is also included. Current technology has evolved from a simple LBS into microwave-assisted LBS, liquid biphasic flotation (LBF), thermoseparation (TMP), three-phase partitioning (TPP), ultrasound-assisted LBS, and electrically assisted LBS. pH, time, temperature, and concentration are some of the significant research parameters considered in the review of advanced techniques.

scholarly journals Alternative Extraction and Downstream Purification Processes for Anthocyanins

Molecules ◽  
2022 ◽  
Vol 27 (2) ◽  
pp. 368
Author(s):  
Ana N. Nunes ◽  
Alexandra Borges ◽  
Ana A. Matias ◽  
Maria Rosário Bronze ◽  
Joana Oliveira
Keyword(s):  
Cost Effective ◽  
Deep Eutectic Solvents ◽  
Extraction Methods ◽  
Chemical Diversity ◽  
Preparative Scale ◽  
Extraction And Purification ◽  
Cosmetic Industry ◽  
Cost Effective Alternative ◽  
Compressed Fluid ◽  
Counter Current

Anthocyanins are natural pigments displaying different attractive colors ranging from red, violet, to blue. These pigments present health benefits that increased their use in food, nutraceuticals, and the cosmetic industry. However, anthocyanins are mainly extracted through conventional methods that are time-consuming and involve the use of organic solvents. Moreover, the chemical diversity of the obtained complex extracts make the downstream purification step challenging. Therefore, the growing demand of these high-value pigments has stimulated the interest in designing new, safe, cost-effective, and tunable strategies for their extraction and purification. The current review focuses on the potential application of compressed fluid-based (such as subcritical and supercritical fluid extraction and pressurized liquid extraction) and deep eutectic solvents-based extraction methods for the recovery of anthocyanins. In addition, an updated review of the application of counter-current chromatography for anthocyanins purification is provided as a faster and cost-effective alternative to preparative-scale HPLC.

scholarly journals EXTRACTION OF COLLAGENOLYTIC ENZYME FROM FISH VISCERA BY PHASE PARTITIONING (PEG/CITRATE) AND ITS POTENTIAL FOR INDUSTRIAL APPLICATION

2021 ◽  
Vol 46 (4) ◽  
pp. 1-8
Author(s):  
Jessica Costa SILVA ◽  
Luiz Henrique Svintiskas LINO ◽  
Márcia Nieves CARNEIRO DA CUNHA ◽  
Juanize Matias da Silva BATISTA ◽  
Vagne Melo OLIVEIRA ◽  
...  
Keyword(s):  
Low Cost ◽  
Aqueous System ◽  
Added Value ◽  
Collagenolytic Activity ◽  
Two Phase ◽  
Phase Partitioning ◽  
Centropomus Undecimalis ◽  
Common Snook ◽  
Extraction And Purification ◽  
Fish Viscera

Internal viscera fish are potential sources of protein biomolecules of biopharmaceutical interest. However, this residue is frequently discarded inappropriately. The possibility to obtain by products of higher added value is a reality. Inside this view attention must be given to processes for the recovery and extraction of target molecules. However, the high cost of processing these residues is one of the obstacles to their reuse; techniques that facilitate their handling and make the process cheaper are desirable, such as extraction in a two-phase aqueous system. Thus, the aim of this study was to extract collagenolytic enzymes from common snook (Centropomus undecimalis) using a two-phase aqueous system (polyethylene glycol/citrate), according to the 24 factorial design, using as variables: molar mass of PEG (MPEG), PEG concentration (CPEG), citrate concentration (CCIT), pH, still, considering purification factor (FP), partition coefficient (K), and yield (Y). The collagenolytic activity of the crude extract was 102.41 U mg-1, after partitioning, was purified 3.91 times (MPEG: 8000; CPEG: 20.0%; CCIT: 20.0% and pH 6.0). Inhibition (U mg-1) was observed in benzamidine (22.51), TLCK (21.05), TPCK (21.29), and PMSF (23.05), signaling to be a serine-protease. The results showed the advantage of this semipurification technique as concerns to the low cost of extraction and purification, adding value to the fishing source material and allocating the residues from its processing to the industry.

scholarly journals Preparative Purification of Recombinant Proteins: Current Status and Future Trends

2013 ◽  
Vol 2013 ◽  
pp. 1-18 ◽  
Author(s):  
Mayank Saraswat ◽  
Luca Musante ◽  
Alessandra Ravidá ◽  
Brian Shortt ◽  
Barry Byrne ◽  
...  
Keyword(s):  
High Performance ◽  
Cost Effective ◽  
Downstream Processing ◽  
Current Status ◽  
Comprehensive Overview ◽  
Two Phase ◽  
Phase Partitioning ◽  
Absolute Requirement ◽  
Flow Filtration ◽  
Selection Of

Advances in fermentation technologies have resulted in the production of increased yields of proteins of economic, biopharmaceutical, and medicinal importance. Consequently, there is an absolute requirement for the development of rapid, cost-effective methodologies which facilitate the purification of such products in the absence of contaminants, such as superfluous proteins and endotoxins. Here, we provide a comprehensive overview of a selection of key purification methodologies currently being applied in both academic and industrial settings and discuss how innovative and effective protocols such as aqueous two-phase partitioning, membrane chromatography, and high-performance tangential flow filtration may be applied independently of or in conjunction with more traditional protocols for downstream processing applications.

Long-term stability of organic–inorganic hybrid perovskite solar cells with high efficiency under high humidity conditions

2017 ◽  
Vol 5 (4) ◽  
pp. 1374-1379 ◽  
Author(s):  
Yue Sun ◽  
Yihui Wu ◽  
Xiang Fang ◽  
Linjun Xu ◽  
Zhijie Ma ◽  
...  
Keyword(s):  
Solar Cells ◽  
High Efficiency ◽  
Perovskite Solar Cells ◽  
Cost Effective ◽  
Water Soluble ◽  
Inorganic Hybrid ◽  
Term Stability ◽  
Long Term Stability ◽  
Hybrid Perovskite

Perovskite solar cells with superior tolerance to humidity (85–95% RH) and long-term stability have been achieved via adding a certain amount of a cost-effective and available water soluble additive, polyvinyl alcohol (PVA).

scholarly journals Oil Palm’s Empty Fruit Bunch as a Sorbent Material in Filter System for Oil-Spill Clean Up

Plants ◽  
2022 ◽  
Vol 11 (1) ◽  
pp. 127
Author(s):  
Nurul Aini Puasa ◽  
Siti Aqlima Ahmad ◽  
Nur Nadhirah Zakaria ◽  
Khalilah Abdul Khalil ◽  
Siti Hajar Taufik ◽  
...  
Keyword(s):  
Oil Spill ◽  
High Efficiency ◽  
Oil Pollution ◽  
Agricultural Waste ◽  
Cost Effective ◽  
Renewable Resource ◽  
Empty Fruit Bunch ◽  
Treatment Conditions ◽  
Predicted Values ◽  
Experimental Values

Oil pollution such as diesel poses a significant threat to the environment. Due to this, there is increasing interest in using natural materials mainly from agricultural waste as organic oil spill sorbents. Oil palm’s empty fruit bunch (EFB), a cost-effective material, non-toxic, renewable resource, and abundantly available in Malaysia, contains cellulosic materials that have been proven to show a good result in pollution treatment. This study evaluated the optimum screening part of EFB that efficiently absorbs oil and the physicochemical characterisation of untreated and treated EFB fibre using Fourier Transform Infrared Spectroscopy (FTIR) and Scanning Electron Microscopy (SEM). The treatment conditions were optimised using one-factor-at-a-time (OFAT), which identified optimal treatment conditions of 170 °C, 20 min, 0.1 g/cm3, and 10% diesel, resulting in 23 mL of oil absorbed. The predicted model was highly significant in statistical Response Surface Methodology (RSM) and confirmed that all the parameters (temperature, time, packing density, and diesel concentration) significantly influenced the oil absorbed. The predicted values in RSM were 175 °C, 22.5 min, 0.095 g/cm3, and 10%, which resulted in 24 mL of oil absorbed. Using the experimental values generated by RSM, 175 °C, 22.5 min, 0.095 g/cm3, and 10%, the highest oil absorption achieved was 24.33 mL. This study provides further evidence, as the data suggested that RSM provided a better approach to obtain a high efficiency of oil absorbed.

Urban pollution control strategy: SBR technology to treat agricultural waste from pig farm in Indonesia

2019 ◽  
Vol 2 (2) ◽  
pp. 21
Author(s):  
Lindawati Lindawati
Keyword(s):  
Cost Estimation ◽  
Batch Reactor ◽  
Agricultural Waste ◽  
Cost Effective ◽  
Land Application ◽  
Pig Manure ◽  
Small Scale ◽  
Piggery Wastewater ◽  
Pig Farm ◽  
Number Of Customers

Reduction of food rations and shortages is one of the impacts of the increasing human population. Food sector industries then try to cope with the fast growing number of customers. Agribusiness sector gains its popularity in these recent years, including pig farm. The increase trend of animal farming industry is likely to bring increasing pollution problem unless effective treatment methods are used. The main problems related to the pig farm include odor nuisance and pig manure disposal. The existing land application of piggery wastewater is the traditional way to discharge the wastewater. This may yield in land and water contamination, due to the accumulation of unused nutrients by crop plant. A case study of a large commercial pig farm from Australia is proposed to apply in smaller scale in Indonesia. Operational strategies for the small-scale SBR (Sequencing Batch Reactor) treating piggery effluent were developed based on lab-scale experiments. Due to SBR characteristics, which are money-saving and space-saving, it is very suitable to be applied in urban area. An economic evaluation was made of various process options. The cost estimation showed that SBR is a cost effective process, allowing operational batches to be adjusted to reduce unnecessary aeration cost. A reduction in the aeration cost was achieved by shortening the batch time from 24-h to 8-h. A comparison of three different SBR options showed that smaller size reactors could be more flexible and cost effective when compared with the larger ones.

Apparatus for aqueous two-phase partitioning for terrestrial and space applications

1995 ◽  
Author(s):  
Mark Deuser ◽  
John Vellinger ◽  
Robert Naumann ◽  
Martin Guinn ◽  
Paul Todd
Keyword(s):  
Two Phase ◽  
Space Applications ◽  
Phase Partitioning
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