scholarly journals Ethnopharmacological Survey and Comparative Study of the Healing Activity of Moroccan Thyme Honey and Its Mixture with Selected Essential Oils on Two Types of Wounds on Albino Rabbits

Foods ◽  
2021 ◽  
Vol 11 (1) ◽  
pp. 28
Author(s):  
Mouna Mekkaoui ◽  
Hamza Assaggaf ◽  
Ahmed Qasem ◽  
Adel El-Shemi ◽  
Emad M. Abdallah ◽  
...  
Keyword(s):  
Wound Healing ◽  
Essential Oils ◽  
Healing Time ◽  
Skin Wound ◽  
Thymus Vulgaris ◽  
Standard Group ◽  
Bacterial Strains ◽  
Skin Wound Healing ◽  
Treatment Groups ◽  
Healing Effect

Wound healing consists of several continuous phases involving various cells and chemical intermediates. As a rich source of nutrition elements, honey has proved to have potential benefits in the treatment of various diseases. The present study was designed to investigate the healing effect of a honey mixture with selected essential oils on chemical and thermal wound models in rabbits. Dressing mixtures of Thymus vulgaris honey with three essential oils (Origanum vulgare, Rosmarinus officinalis, and Thymus vulgaris) were prepared and applied daily in the treatment groups. These essential oils were rich in phytochemicals and had significant antibacterial activity against four selected ATCC bacterial strains. Madecasol ointment was used as a standard control. The healing effect of the mixtures was evaluated by measuring wound surface area and comparing healing time. The results showed that the healing rate in the treatment groups was significantly higher than that of the untreated group and standard group. The best healing effect for burns was seen in the mixture of honey and Thymus vulgaris essential oil, which had wound closure rates of 85.21% and 82.14% in thermal- and chemical-induced burns, respectively, and showed the shortest healing time (14 days) in comparison to other groups. Therefore, it can be concluded that honey mixtures have significant beneficial effects on skin wound healing and, thus, they may be used as a healing agent in different types of wounds in humans after specific clinical trials.

scholarly journals Carbonic Anhydrase VI in Skin Wound Healing Study on Car6 Knockout Mice

2020 ◽  
Vol 21 (14) ◽  
pp. 5092
Author(s):  
Toini Pemmari ◽  
Jaakko Laakso ◽  
Maarit S. Patrikainen ◽  
Seppo Parkkila ◽  
Tero A. H. Järvinen
Keyword(s):  
Wound Healing ◽  
Knockout Mice ◽  
Wound Closure ◽  
Skin Wound ◽  
Carbonic Anhydrases ◽  
Skin Wound Healing ◽  
Tumor Cell Migration ◽  
Treatment Groups ◽  
Thickness Skin ◽  
Carbonic Anhydrase Vi

Carbonic anhydrases (CAs) contribute to tumor cell migration by generating an acidic environment through the conversion of carbon dioxide to bicarbonate and a proton. CA VI is secreted to milk and saliva, and it could contribute to wound closure, as a potential trophic factor, in animals that typically lick their wounds. Our aim was to investigate whether human CA VI improves skin-wound healing in full-thickness skin-wound models. The effect was studied in Car6 −/− knockout mice and wild type littermates. Half of both mice strains were given topically administered, milk-derived CA VI after wounding and eight hours later. The amount of topically given CA VI exceeded the predicted amount of natural saliva-delivered CA VI. The healing was followed for seven days and studied from photographs and histological sections. Our results showed no significant differences between the treatment groups in wound closure, re-epithelization, or granulation tissue formation, nor did the Car6 genotype affect the healing. Our results demonstrate that CA VI does not play a major role in skin-wound healing and also suggest that saliva-derived CA VI is not responsible for the licking-associated improved wound healing in animals.

scholarly journals From Inflammation to Cutaneous Repair: Topical Application of Lupeol Improves Skin Wound Healing in Rats by Modulating the Cytokine Levels, NF-κB, Ki-67, Growth Factor Expression, and Distribution of Collagen Fibers

2020 ◽  
Vol 21 (14) ◽  
pp. 4952 ◽  
Author(s):  
Fernando Pereira Beserra ◽  
Lucas Fernando Sérgio Gushiken ◽  
Ana Júlia Vieira ◽  
Danilo Augusto Bérgamo ◽  
Patrícia Luísa Bérgamo ◽  
...  
Keyword(s):  
Gene Expression ◽  
Wound Healing ◽  
Growth Factor ◽  
Skin Wound ◽  
Collagen Fibers ◽  
Skin Wound Healing ◽  
Ki 67 ◽  
Healing Effect ◽  
Cytokine Levels ◽  
Wound Healing Effect

Skin wound healing is a highly complex event that involves different mediators at the cellular and molecular level. Lupeol has been reported to possess different biological activities, such as anti-inflammatory, antioxidant, antidiabetic, and in vitro wound healing properties, which motivated us to proceed with in vivo studies. We aimed to investigate the wound healing effect of lupeol-based cream for 3, 7, and 14 days. Wound excisions were induced on the thoraco-lumbar region of rats and topically treated immediately after injury induction. Macroscopic, histopathological, and immunohistochemical analyses were performed. Cytokine levels were measured by ELISA and gene expression was evaluated by real-time RT-qPCR. Our results showed a strong wound-healing effect of lupeol-based cream after 7 and 14 days. Lupeol treatment caused a reduction in proinflammatory cytokines (TNF-a, IL-1β, and IL-6) and gene and protein NF-κB expression, and positively altered IL-10 levels, showing anti-inflammatory effects in the three treatment periods. Lupeol treatment showed involvement in the proliferative phase by stimulating the formation of new blood vessels, increasing the immunostaining of Ki-67 and gene expression, and immunolabeling of vascular endothelial growth factor (VEGF) and epidermal growth factor (EGF), and increasing gene expression of transforming growth factor beta-1 (TGF-β1) after seven days of treatment. Lupeol was also involved in the tissue regeneration phase by increasing the synthesis of collagen fibers noted in the three treatment periods analyzed. Our findings suggest that lupeol may serve as a novel therapeutic option to treat cutaneous wounds by regulating mechanisms involved in the inflammatory, proliferative, and tissue-remodeling phases.

scholarly journals The Effects of Hydrogen-Rich Water on Skin Wound Healing in Dogs

2021 ◽  
Author(s):  
Dong-Dong Qi ◽  
Ting Wang ◽  
Muhammad Abid Hayat ◽  
Tao Liu ◽  
JianTao Zhang
Keyword(s):  
Wound Healing ◽  
Blood Vessels ◽  
Healing Time ◽  
Skin Wound ◽  
Control Group ◽  
Sod Activity ◽  
Skin Wound Healing ◽  
Histopathological Results ◽  
Wound Healing Time ◽  
Skin Homogenate

Abstract Background: Hydrogen-rich water (HRW) has been shown to be one of underlying therapeutic strategies regarding wound treatments. This study explored the effects of drinking HRW on skin wound healing in dogs. Eight circular wounds were analyzed in each dog. The experimental group was treated with HRW twice daily, while the control group was provided with distilled water (DW). The wound tissues of dogs examined histopathologically. The fibroblasts, inflammatory cell infiltration, the average number of new blood vessels, and the level of malondialdehyde (MDA) and superoxide dismutase (SOD) activity in the skin homogenate of the wound was measured using the corresponding kits. The expression of Nrf-2, HO-1, NQO-1, VEGF, and PDGF were measured by real-time fluorescence quantitative method. Results: We observed that HRW wound had significant average healing rate and faster average healing time. Histopathological results showed that the average number of blood vessels and the average thickness of epidermis were significantly different from the DW group. The MDA levels were higher in the DW group than in the HRW group but the SOD levels were higher in the HRW group than in the DW group. The results of qRT-PCR showed that the expression of each gene was significantly different between the two groups. Conclusions: The results showed that HRW treatment could promote skin wound healing in dogs, accelerate wound epithelization, reduce inflammatory reaction, stimulate the expression of cytokines related to wound healing, and shorten wound healing time.

scholarly journals Ulmus Parvifolia Accelerate Skin Wound Healing by Regulating the Expression of MMPs and TGF-β

2019 ◽  
Author(s):  
Min Cheol Kang ◽  
Silvia Yumnam ◽  
Woo Sung Park ◽  
Hae Min So ◽  
Ki Hyun Kim ◽  
...  
Keyword(s):  
Wound Healing ◽  
Growth Factor ◽  
Transforming Growth Factor ◽  
Skin Wound ◽  
Root Bark ◽  
Potential Candidate ◽  
Skin Wound Healing ◽  
Healing Effect ◽  
Ulmus Parvifolia ◽  
Wound Healing Effect

Ulmus species have been widely used in Korean folk medicine because of their anti-inflammatory and antimicrobial properties. We intended to investigate the wound healing effect of the powder of Ulmus parvifolia (UP) root bark in a mouse wound healing model. We also determined the mechanisms of effects of Ulmus parvifolia (UP) in skin and skin wound healing effect using keratinocyte model. in vivo experiments showed that the wound lesions in the mice decreased by U. parvifolia with 200 mesh size of root bark powder and significantly reduced by treatment with UP, compared with those treated with U. macrocarpa (UM). Results from in vitro experiments also revealed that UP extract promoted the migration of human skin keratinocytes. UP powder treatment upregulated the expression of the matrix metalloproteinase-2 and -9 protein and significantly increased transforming growth factor (TGF)-β levels. We confirmed that topical administration of the bark powder of exerted a significant effect on skin wound healing by upregulating the expression of MMP and transforming growth factor-β. TGF-β In, Our study suggests that U. parvifolia may be a potential candidate for skin wound healing including epidermal skin rejuvernation.

Mesenchymal stem cells' interaction with skin: Wound-healing effect on fibroblast cells and skin tissue

2010 ◽  
Vol 18 (6) ◽  
pp. 655-661 ◽  
Author(s):  
Young Keul Jeon ◽  
Yun Ho Jang ◽  
Dong Ryeol Yoo ◽  
Si Na Kim ◽  
Sang Koo Lee ◽  
...  
Keyword(s):  
Stem Cells ◽  
Wound Healing ◽  
Mesenchymal Stem Cells ◽  
Skin Wound ◽  
Skin Tissue ◽  
Fibroblast Cells ◽  
Skin Wound Healing ◽  
Healing Effect ◽  
Wound Healing Effect

Wound healing effect benzosulfonate 1-ethyl-3-methyl-4,5-bis(n-methylcarbamoyl) imidazolium

2020 ◽  
Vol 18 (3) ◽  
pp. 229-235
Author(s):  
Lydmila K. Khnychenko ◽  
Elena N. Selina ◽  
Olga M. Rodionova ◽  
Levon B. Piotrovskiy ◽  
Petr D. Shabanov
Keyword(s):  
Wound Healing ◽  
Healing Process ◽  
Experimental Models ◽  
Skin Wound ◽  
Complete Healing ◽  
Male Rats ◽  
Skin Wounds ◽  
Skin Wound Healing ◽  
Healing Effect ◽  
Wound Healing Effect

Materials and methods. In experiments on 180 male rats weighing 180200 g, the wound-healing effect of derivatives of imidazole (IEM-1181) was evaluated as a 10% ointment on models of aseptic full-layer linear and planar skin wounds. Results. It was found that the compound IEM-1181 in the form of 10% ointment has a pronounced on skin wound healing effect, manifested in the qualitative features of the regenerative healing process. With local application of the ointment containing the tested compound, the strength of the tissue formed on the site of a full-layer linear skin wound was increased when its edges were stretched, and the healing time of full-layer planar wounds was reduced. The morphological picture of the tissue formed at the site of the wound defect corresponded to complete healing by primary tension with complete epithelization of the surface and squamous cell differentiation of the epithelial regenerate without signs of inflammation and scar formation. Conclusion. The results of the study on experimental models of full-layer linear and planar skin wounds indicate that the wound healing effect of the tested ointment is due to the anti-inflammatory activity of the IEM-1181 compound

scholarly journals The Therapeutic Effects of Oral Intake of Hydrogen Rich Water on Cutaneous Wound Healing in Dogs

2021 ◽  
Vol 8 (11) ◽  
pp. 264
Author(s):  
Dong-Dong Qi ◽  
Meng-Yuan Ding ◽  
Ting Wang ◽  
Muhammad Abid Hayat ◽  
Tao Liu ◽  
...  
Keyword(s):  
Wound Healing ◽  
Blood Vessels ◽  
Oral Intake ◽  
Healing Time ◽  
Skin Wound ◽  
Control Group ◽  
Therapeutic Effects ◽  
Sod Activity ◽  
Skin Wound Healing ◽  
Wound Healing Time

This study explored the effects of drinking Hydrogen-rich water (HRW) on skin wound healing in dogs. Eight circular wounds were analyzed in each dog. The experimental group was treated with HRW thrice daily, while the control group was provided with distilled water (DW). The wound tissues of dogs were examined histopathologically. The fibroblasts, inflammatory cell infiltration, the average number of new blood vessels, and the level of malondialdehyde (MDA) and superoxide dismutase (SOD) activity in the skin homogenate of the wound was measured using the corresponding kits. The expressions of Nrf-2, HO-1, NQO-1, VEGF, and PDGF were measured using the real-time fluorescence quantitative method. We observed that HRW wounds showed an increased rate of wound healing, and a faster average healing time compared with DW. Histopathology showed that in the HRW group, the average thickness of the epidermis was significantly lower than the DW group. The average number of blood vessels in the HRW group was higher than the DW group. The MDA levels were higher in the DW group than in the HRW group, but the SOD levels were higher in the HRW group than in the DW group. The results of qRT-PCR showed that the expression of each gene was significantly different between the two groups. HRW treatment promoted skin wound healing in dogs, accelerated wound epithelization, reduced inflammatory reaction, stimulated the expression of cytokines related to wound healing, and shortened wound healing time.

scholarly journals Characterization and Topical Study of Aloe Vera Hydrogel on Wound-Healing Process

Polymers ◽  
2021 ◽  
Vol 13 (22) ◽  
pp. 3958
Author(s):  
Karen Zulema Meza-Valle ◽  
Rosa Alicia Saucedo-Acuña ◽  
Karla Lizzette Tovar-Carrillo ◽  
Juan Carlos Cuevas-González ◽  
Erasto Armando Zaragoza-Contreras ◽  
...  
Keyword(s):  
Wound Healing ◽  
Healing Process ◽  
Aloe Vera ◽  
Healing Time ◽  
Skin Wound ◽  
Swelling Kinetics ◽  
Wound Healing Process ◽  
Sem Images ◽  
Skin Wound Healing

Wound healing is fundamental to restore the tissue integrity. A topical study of the influence of Aloe vera hydrogel, formulated with 1,2-propanediol (propanediol) and triethanolamine (TEA), on the skin wound-healing process was investigated in female Wistar rats. FTIR spectroscopy confirms the presence of carboxylic acid and methyl ester carboxylate groups related with important compounds that confer the hydrogel a good interaction with proteins and growth factors. SEM images show a microstructure and micro-roughness that promote a good adhesion to the wound. Therefore, the swelling kinetics and the contact angle response contribute to the understanding of the in vivo results of the animal test. The results indicated that the Aloe vera hydrogel, prepared with propanediol and TEA, together with its superficial characteristics, improve its rapid penetration without drying out the treated tissue. This produced a positive influence on inflammation, angiogenesis, and wound contraction, reducing 29% the total healing time, reaching the total closure of the wound in 15 days.

scholarly journals Overexpression of the Oral Mucosa-Specific microRNA-31 Promotes Skin Wound Closure

2019 ◽  
Vol 20 (15) ◽  
pp. 3679 ◽  
Author(s):  
Lin Chen ◽  
Alyne Simões ◽  
Zujian Chen ◽  
Yan Zhao ◽  
Xinming Wu ◽  
...  
Keyword(s):  
Wound Healing ◽  
Oral Mucosa ◽  
Wound Closure ◽  
Expression Patterns ◽  
Skin Wound ◽  
Skin Wound Healing ◽  
Specific Expression ◽  
Keratinocyte Proliferation

Wounds within the oral mucosa are known to heal more rapidly than skin wounds. Recent studies suggest that differences in the microRNAome profiles may underlie the exceptional healing that occurs in oral mucosa. Here, we test whether skin wound-healing can be accelerating by increasing the levels of oral mucosa-specific microRNAs. A panel of 57 differentially expressed high expresser microRNAs were identified based on our previously published miR-seq dataset of paired skin and oral mucosal wound-healing [Sci. Rep. (2019) 9:7160]. These microRNAs were further grouped into 5 clusters based on their expression patterns, and their differential expression was confirmed by TaqMan-based quantification of LCM-captured epithelial cells from the wound edges. Of these 5 clusters, Cluster IV (consisting of 8 microRNAs, including miR-31) is most intriguing due to its tissue-specific expression pattern and temporal changes during wound-healing. The in vitro functional assays show that ectopic transfection of miR-31 consistently enhanced keratinocyte proliferation and migration. In vivo, miR-31 mimic treatment led to a statistically significant acceleration of wound closure. Our results demonstrate that wound-healing can be enhanced in skin through the overexpression of microRNAs that are highly expressed in the privileged healing response of the oral mucosa.

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