Bioconversion of Fish Discards through the Production of Lactic Acid Bacteria and Metabolites: Sustainable Application of Fish Peptones in Nutritive Fermentation Media

In the current work, we study the capacity of 30 peptones obtained by enzyme proteolysis of ten discarded fish species (hake, megrim, red scorpionfish, pouting, mackerel, gurnard, blue whiting, Atlantic horse mackerel, grenadier, and boarfish) to support the growth and metabolite production of four lactic acid bacteria (LAB) of probiotic and technological importance. Batch fermentations of Lactobacillus plantarum, L. brevis, L. casei, and Leuconostoc mesenteroides in most of the media formulated with fish peptones (87% of the cases) led to similar growths (quantified as dry-weight biomass and viable cells) and metabolites (mainly lactic acid) than in commercial control broth (MRS). Comparisons among cultures were performed by means of the parameters obtained from the mathematical fittings of experimental kinetics to the logistic equation. Modelling among experimental and predicted data from each bioproduction was generally accurate. A simple economic assessment demonstrated the profitability achieved when MRS is substituted by media formulated with fish discards: a 3–4-fold reduction of costs for LAB biomass, viable cells formation, and lactic and acetic acid production. Thus, these fish peptones are promising alternatives to the expensive commercial peptones as well as a possible solution to valorize discarded fish biomasses and by-products.

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Co-Microencapsulated Black Rice Anthocyanins and Lactic Acid Bacteria: Evidence on Powders Profile and In Vitro Digestion

Molecules ◽

10.3390/molecules26092579 ◽

2021 ◽

Vol 26 (9) ◽

pp. 2579

Author(s):

Carmen-Alina Bolea ◽

Mihaela Cotârleț ◽

Elena Enachi ◽

Vasilica Barbu ◽

Nicoleta Stănciuc

Keyword(s):

Lactic Acid ◽

Lactic Acid Bacteria ◽

Oryza Sativa L ◽

Milk Proteins ◽

Ethanolic Extract ◽

Dry Weight ◽

Hot Water ◽

Raw Material ◽

Black Rice

Two multi-functional powders, in terms of anthocyanins from black rice (Oryza sativa L.) and lactic acid bacteria (Lactobacillus paracasei, L. casei 431®) were obtained through co-microencapsulation into a biopolymer matrix composed of milk proteins and inulin. Two extracts were obtained using black rice flour as a raw material and hot water and ethanol as solvents. Both powders (called P1 for aqueous extract and P2 for ethanolic extract) proved to be rich sources of valuable bioactives, with microencapsulation efficiency up to 80%, both for anthocyanins and lactic acid bacteria. A higher content of anthocyanins was found in P1, of 102.91 ± 1.83 mg cyanindin-3-O-glucoside (C3G)/g dry weight (DW) when compared with only 27.60 ± 17.36 mg C3G/g DW in P2. The morphological analysis revealed the presence of large, thin, and fragile structures, with different sizes. A different pattern of gastric digestion was observed, with a highly protective effect of the matrix in P1 and a maximum decrease in anthocyanins of approximatively 44% in P2. In intestinal juice, the anthocyanins decreased significantly in P2, reaching a maximum of 97% at the end of digestion; whereas in P1, more than 45% from the initial anthocyanins content remained in the microparticles. Overall, the short-term storage stability test revealed a release of bioactive from P2 and a decrease in P1. The viable cells of lactic acid bacteria after 21 days of storage reached 7 log colony forming units (CFU)/g DW.

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Physicochemical, microbiological, and sensory characteristics of probiotic Bulgarian yoghurts obtained by ultrafiltration of goat’s milk

Zeitschrift für Naturforschung C ◽

10.1515/znc-2021-0064 ◽

2021 ◽

Vol 0 (0) ◽

Author(s):

Mariya Dushkova ◽

Siyka Kodinova ◽

Zapryana Denkova ◽

Velichka Yanakieva ◽

Nikolay Dimitrov Menkov

Keyword(s):

Lactic Acid ◽

Lactic Acid Bacteria ◽

Lactobacillus Acidophilus ◽

Dry Matter ◽

Titratable Acidity ◽

Bifidobacterium Bifidum ◽

Sensory Characteristics ◽

Viable Cells ◽

Goat’S Milk ◽

Goat's Milk

Abstract The purpose of this study was to investigate the microbiological (number of viable lactic acid bacteria and bifidobacteria), physicochemical (dry matter, protein and fat contents, titratable acidity, and pH), and sensory characteristics (appearance of coagulum, taste and aroma, structure at cutting, color, and consistency at shattering) of probiotic Bulgarian yoghurts obtained by ultrafiltration of goat’s milk. These yoghurts were obtained using volume reduction ratios of 2 and 3 with the probiotic starters MZ2f, MZ2f + Bifidobacterium bifidum BB – 87, and MZ2f + Lactobacillus acidophilus LAB – 8. The increase in the level of the concentration by ultrafiltration led to an increase in the dry matter, protein and fat contents of the yoghurts, in the number of lactic acid bacteria and titratable acidity, and to a decrease in the pH. The twofold concentration by ultrafiltration resulted in a higher number of lactic acid bacteria in comparison with yoghurts made without ultrafiltration, and with better sensory characteristics compared to yoghurts without and with threefold ultrafiltration. The higher number of viable cells and better sensory characteristics were obtained for yoghurts with MZ2f + Bifidobacterium bifidum BB – 87 and MZ2f + Lactobacillus acidophilus LAB – 8 in comparison with MZ2f alone.

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Influence of thermophilic lactic acid bacteria strains on propionibacteria growth and lactate consumption in an Emmental juice-like medium

Journal of Dairy Research ◽

10.1017/s0022029998003331 ◽

1999 ◽

Vol 66 (1) ◽

pp. 105-113 ◽

Cited By ~ 11

Author(s):

ANNE THIERRY ◽

DELPHINE SALVAT-BRUNAUD ◽

JEAN-LOUIS MAUBOIS

Keyword(s):

Amino Acids ◽

Lactic Acid ◽

Lactic Acid Bacteria ◽

Free Amino Acids ◽

Free Amino ◽

Streptococcus Thermophilus ◽

Lactobacillus Helveticus ◽

The Media ◽

Lactate Consumption ◽

Thermophilic Lactic Acid Bacteria

Swiss-type cheeses such as Emmental are characterized by the successive development of thermophilic lactic acid bacteria (TLAB) and propionibacteria. The aim of this study was to determine whether the choice of TLAB strain influenced propionibacteria. TLAB and propionibacteria were cultured sequentially under the conditions prevailing in cheese. Firstly, 11 Emmental juice-like media were prepared by fermenting casein-enriched milk with pure or mixed cultures of TLAB (Lactobacillus helveticus, Lb. delbrueckii subsp. lactis and Streptococcus thermophilus), differing in their proteolytic activities. TLAB cells were then removed by microfiltration. Finally, five strains of Propionibacterium freudenreichii were grown on these media at 24°C under anaerobiosis and their growth characteristics and lactate consumption determined. The media mainly differed in their contents of peptides (1·9–5·3 g/kg) and free amino acids (1·0–5·6 g/kg) and the proportions of lactate isomers (42–92% of the L(+) isomer). Propionibacteria were significantly (P<0·05) influenced by TLAB strains (differences in doubling times of up to 20% and differences in lactate consumption after 600 h culture of up to 52%). The influence of TLAB was similar for all the propionibacteria tested, depended on the TLAB strains and could not be generalized to the TLAB species. Propionibacteria were stimulated by high peptide levels, low levels of free amino acids and NaCl, a low proportion of L(+)-lactate and other undetermined factors. However, variations due to TLAB were less than those between propionibacteria strains.

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Cholesterol Assimilation by Lactic Acid Bacteria and Bifidobacteria Isolated from the Human Gut

Applied and Environmental Microbiology ◽

10.1128/aem.68.9.4689-4693.2002 ◽

2002 ◽

Vol 68 (9) ◽

pp. 4689-4693 ◽

Cited By ~ 231

Author(s):

Dora I. A. Pereira ◽

Glenn R. Gibson

Keyword(s):

Lactic Acid ◽

Lactic Acid Bacteria ◽

Bile Acids ◽

Dry Weight ◽

Human Gut ◽

Healthy Human ◽

Bacterial Populations ◽

Acid And Bile Tolerance ◽

Commercial Probiotics

ABSTRACT The objective of this study was to evaluate the effect of human gut-derived lactic acid bacteria and bifidobacteria on cholesterol levels in vitro. Continuous cultures inoculated with fecal material from healthy human volunteers with media supplemented with cholesterol and bile acids were used to enrich for potential cholesterol assimilators among the indigenous bacterial populations. Seven potential probiotics were found: Lactobacillus fermentum strains F53 and KC5b, Bifidobacterium infantis ATCC 15697, Streptococcus bovis ATCC 43143, Enterococcus durans DSM 20633, Enterococcus gallinarum, and Enterococcus faecalis. A comparative evaluation regarding the in vitro cholesterol reduction abilities of these strains along with commercial probiotics was undertaken. The degree of acid and bile tolerance of strains was also evaluated. The human isolate L. fermentum KC5b was able to maintain viability for 2 h at pH 2 and to grow in a medium with 4,000 mg of bile acids per liter. This strain was also able to remove a maximum of 14.8 mg of cholesterol per g (dry weight) of cells from the culture medium and therefore was regarded as a candidate probiotic.

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Uji Daya Hidup Bakteri Asam Laktat Dari Usus Itik Pada Media Tumbuh Dengan Penambahan Variasi Konsentrasi Molasis

Jurnal Penelitian Pertanian Terapan ◽

10.25181/jppt.v16i1.74 ◽

2017 ◽

Vol 16 (1) ◽

Author(s):

Rudy Sutrisna ◽

Christina Nugroho Ekowati ◽

Riska Damayanti

Keyword(s):

Lactic Acid ◽

Lactic Acid Bacteria ◽

Key Words ◽

Population Size ◽

Total Population ◽

Concentration Variation ◽

The Media ◽

And Control ◽

Mrs Medium

This study aims to determine differences in the viability of Lactic Acid Bacteria (LAB) isolates on MRS medium supplemented with various concentrations molasses. Concentration variation molasis used as controls were 0%, 1%, 2% and 3%. Variation of time incubation on each of the media that is 0 hours, 24 hours, 48 hours and 72 hours. The viability of each isolate was viewed with the population of Log (cells/ml) which grow on the medium and the number of generations and time of generations. The results showed that the concentration molasses added to MRS affect the viability of LAB. Concentration of 1% makes LAB better viability than controls. Total population continues to increase at the end of the incubation with a yield of 1.17 generations in time 20.46 hours/generation. In contrast to controls, reduced population size at the end of the incubation so as not to produce a generation. LAB viability in addition molasses 2% and 3% live on 48 hours of incubation. This is indicated by the number of population increased at 48 hours of incubation, and then decreased at the end of incubation. The addition of concentration variation in the types of bacteria showed different viability. At B1 adding molasses 2% and 3% live on 48 hours of incubation and control can only live on a 24 hours incubation, whereas the B3, the control can remain alive at 48 hours of incubation. In contrast to the controls on B4 can survive on 48 hours of incubation, whereas the addition of 3% molasses can only live on a 24 hours incubation. Key words : Lactic Acid Bacteria/LAB, Molasses, Viability

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ANALISIS VIABILITAS LACTOBACILLUS LACTIS PADA INOVASI MEDIA DASAR PERTUMBUHAN ALTERNATIF DAN MEDIA DASAR PENEPUNGAN BAKTERI ASAM LAKTAT

Jurnal TAMBORA ◽

10.36761/jt.v4i2.635 ◽

2020 ◽

Vol 4 (2) ◽

pp. 16-22

Author(s):

Ani Sulastri ◽

Baso Manguntungi

Keyword(s):

Lactic Acid ◽

Lactic Acid Bacteria ◽

Shelf Life ◽

Bacterial Growth ◽

Alternative Media ◽

Plate Count ◽

Growth Media ◽

Media Formulation ◽

The Media ◽

Lactobacillus Lactis

The limited shelf life in a food requires a natural preservative so that the food used is not easily damaged and has a longer shelf life, namely by using lactic acid bacteria (BAL) using alternative media. By using lactic acid bacteria, the time in the storage period food products can be extended. The purpose of this study was to determine the viability of the Lactobacillus lactis bacteria on an alternative growth base media and a media on the media of bacteria. Lactic acid bacteria were rejuvenated and culture propagation of 5 ?l was inoculated into 5 mL of MRSB media. Formulation media used for bacterial growth such as whey tofu + 5% sucrose + 1% urea. The alternative media was incubated for 24 hours. Bacterial growth was observed at 0, 4, 8 and 16 hours using the TPC (Total Plate count) method. Various media Lactobacillus lactis bacterial deposition was grown on MRSB media and dried with freeze dry for 48 hours and the viability of Lactobacillus lactis was tested. The basic growth media that can be used are Lactobacillus lactis bacteria, namely whey tofu + sucrose 5% + urea 1% as well as Lactobacillus lactis viability results in various media which are grown on MRS media and various alternative media shows that the media has a 100% carrageenan composition able to maintain the viability of Lactobacillus lactis cells.

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Co-Microencapsulation of Flavonoids from Yellow Onion Skins and Lactic Acid Bacteria Lead to Multifunctional Ingredient for Nutraceutical and Pharmaceutics Applications

Pharmaceutics ◽

10.3390/pharmaceutics12111053 ◽

2020 ◽

Vol 12 (11) ◽

pp. 1053

Author(s):

Ștefania Adelina Milea ◽

Mihaela Aida Vasile ◽

Oana Crăciunescu ◽

Ana-Maria Prelipcean ◽

Gabriela Elena Bahrim ◽

...

Keyword(s):

Lactic Acid ◽

Lactic Acid Bacteria ◽

Value Added ◽

Dry Weight ◽

Natural Antioxidants ◽

In Vitro Digestion ◽

Inhibitory Effect ◽

Coating Materials ◽

Yellow Onion

In this study, flavonoids extracted from yellow onion skins and Lactobacillus casei were encapsulated in a combination of whey protein isolate, inulin and maltodextrin with an encapsulation efficiency of 84.82 ± 0.72% for flavonoids and 72.49 ± 0.11% for lactic acid bacteria. The obtained powder showed a flavonoid content of 89.49 ± 4.12 mg quercetin equivalents/g dry weight (DW) and an antioxidant activity of 39.27 ± 0.45 mM Trolox/g DW. The powder presented a significant antidiabetic and anti-inflammatory potential, with an inhibitory effect on α-amylase, lipase and lipoxygenase of 76.40 ± 2.30%, 82.58 ± 3.36% and 49.01 ± 0.62%, respectively. The results obtained for in vitro digestion showed that the coating materials have a protective effect on the flavonoids release. Cytotoxicity results indicated that the powder was cytocompatible up to a concentration of 500 μg/mL. The functional potential of the powder was tested by adding in a selected food matrix, highlighting a good stability of the phytochemicals, whereas an increase with 1 log cell forming unit (CFU)/g DW was observed after 21 days of storage. The obtained results are promising in the valorization of natural antioxidants in combination with lactic acid bacteria in order to develop multifunctional ingredients with value-added for food and pharmaceutics applications.

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Effect of ultrafiltration of yeast extracts on their ability to promote lactic acid bacteria growth

Canadian Journal of Microbiology ◽

10.1139/w99-089 ◽

1999 ◽

Vol 45 (11) ◽

pp. 891-897 ◽

Cited By ~ 10

Author(s):

H Gaudreau ◽

C P Champagne ◽

J Conway ◽

R Degré

Keyword(s):

Lactic Acid ◽

Lactic Acid Bacteria ◽

Nitrogen Content ◽

Amino Nitrogen ◽

Growth Curves ◽

Maximum Growth ◽

Maximum Growth Rate ◽

Bacteria Growth ◽

The Media ◽

Uf Membranes

Five yeast extracts (YE) were fractionated by ultrafiltration (UF) with 1, 3, and 10 kDa molecular weight cutoff membranes, concentrated by freeze-drying, and the resulting powders of yeast extract filtrates (YEF) were evaluated for their growth-promoting properties on nine cultures of lactic acid bacteria (LAB). There was an increase in α-amino nitrogen content of the YEF powders as the pore size of the UF membranes used to filter the YE solutions decreased. The source of YE had a much greater effect than UF on the growth of LAB. This was also the case for the YEF contents in total and α-amino nitrogen. Growth curves of the LAB showed that maximum growth rate (μmax) data were on average 30% higher with bakers' YE than with brewers' YE, while maximum optical density (ODmax) values were on average 16% higher with bakers' YE. This could be related to the higher nitrogen content of the bakers' YE used in this study. Modification by UF of the YE had no significant influence on the growth of 4 of the 9 LAB strains. The three strains of Lactobacillus casei were negatively influenced by UF, as they did not grow as well in the media containing the YEF obtained after filtering with 1 and 3 kDa membranes. On a total solids basis, the 2.5× retentates from the 10 kDa membrane gave, on average, 4% lower μmax and 5% lower ODmax values as compared to cultures where the corresponding YEF was used as medium supplement. This could also be partially related to the different nitrogen contents of the filtrates and retentates. Key words: Lactococcus, Pediococcus, Lactobacillus, amino acids.

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Potensi Ekstrak Cincau Hijau (Premna oblongifolia Merr.) dalam Menstimulasi Pertumbuhan Bakteri Asam Laktat Saluran Pencernaan

JURNAL REKAYASA DAN MANAJEMEN AGROINDUSTRI ◽

10.24843/jrma.2020.v08.i03.p01 ◽

2020 ◽

Vol 8 (3) ◽

pp. 319

Author(s):

Friska Ekagia Ginting ◽

Nyoman Semadi Antara ◽

I Made Mahaputra Wijaya

Keyword(s):

Lactic Acid ◽

Lactic Acid Bacteria ◽

Dietary Fiber ◽

Digestive Tract ◽

Bacterial Growth ◽

Good Source ◽

Fermentation Time ◽

The Media

The green grass (Premna oblongifolia Merr), is a woody shrub plant mainly found in the forest. The main content of the plant is pectin, so green grass jelly is considered good source of dietary fiber, because the fiber can be fermented properly by digestive microflora (Gallaher, 2000). The fiber contained in green grass extract is believed to stimulate the growth of lactic acid bacteria in the digestive tract. The purpose of this study was to determine the potential of green grass jelly extract in stimulating the growth of digestive bacteria. The research was carried out in a laboratory with the treatment of adding concentrations of green grass jelly extract powder, and glucose as a control. The media used was Yeast-Pepton which added 1% glucose, green cincau extract powder 0.1%, 0.2%, 0.3%, 0.4%, 0.5%. Fermentation was carried out at 37 ° C with 18 hours fermentation time. The results showed that the concentration of the green grass extract 0.2% gave the best total lactic acid bacteria, this could because at this concentration the media had not increased the viscosity which inhibited bacterial growth. Keywords : green gras, pdigestive lactic acid bacteria, ectin.

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Aflatoxin B1 Binding Capacity of Autochthonous Strains of Lactic Acid Bacteria

Journal of Food Protection ◽

10.4315/0362-028x-72.1.189 ◽

2009 ◽

Vol 72 (1) ◽

pp. 189-192 ◽

Cited By ~ 45

Author(s):

MOHAMMAD R. FAZELI ◽

M. HAJIMOHAMMADALI ◽

AZAMOSSADAT MOSHKANI ◽

NASRIN SAMADI ◽

HOSSEIN JAMALIFAR ◽

...

Keyword(s):

Lactic Acid ◽

Lactic Acid Bacteria ◽

Aflatoxin B1 ◽

High Performance ◽

Detrimental Effect ◽

Binding Capacity ◽

Toxin Complex ◽

The Media ◽

The Stability ◽

Strain Dependent

Some foods are prone to contamination with aflatoxins, with detrimental effect on human health. Lactic acid bacteria have been reported to bind aflatoxins and remove them from foods and feeds. Reduction of aflatoxin B1 (AFB1) from the liquid media by the autochthonous lactic acid bacteria (Lactobacillus casei, Lactobacillus plantarum, and Lactobacillus fermentum) isolated from traditional Iranian sourdough and dairy products is reported in the current study. The effect of incubation time on the binding capacity of the strains to AFB1 was also investigated. Duplicates of individual bacteria with population equivalent to 2 × 1010 CFU/ml were incubated in the presence of AFB1 at 37°C for a period of 72 h, and the amounts of unbound AFB1 were quantitated by reverse-phase high-performance liquid chromatography. All the strains were capable of removal of AFB1, and the reduction of AFB1 ranged from 25 to 61% throughout the incubation period. Removal of AFB1 was a rapid process, with approximately 61 and 56% of the toxin taken instantly by L. fermentum and L. plantarum, respectively. Binding was of a reversible nature, and some of the bound AFB1 was released into the media by the repeated centrifugation and resuspension of the cell pellets. The stability of the bacteria–toxin complex was strain dependent, and L. casei was a stronger binder of AFB1 compared with the other bacteria. No toxin release was observed after 24 h. These findings tend to suggest that certain novel probiotic bacteria with high aflatoxin binding capacity could be selected for detoxification of foods.

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