scholarly journals Fabrication and Evaluation of Silk Sericin-Derived Hydrogel for the Release of the Model Drug Berberine

Gels ◽  
2021 ◽  
Vol 7 (1) ◽  
pp. 23
Author(s):  
Chi Yan ◽  
Jianwei Liang ◽  
Hao Fang ◽  
Xizhi Meng ◽  
Jiale Chen ◽  
...  
Keyword(s):  
Antibacterial Activity ◽  
Gelation Time ◽  
Three Dimensional ◽  
Subsequent Development ◽  
Environmental Benefits ◽  
Dimensional Structure ◽  
Manufacturing Processes ◽  
Silk Sericin ◽  
Model Drug

Silk sericin (SS) produced by Bombyx mori is normally discarded as waste in manufacturing processes, which causes environmental pollution. Therefore, investigating the use of silk sericin has economic and environmental benefits. As a three-dimensional structure, the sericin-derived hydrogel was explored in different applications. However, many developed gelation procedures raise concerns regarding safety, cost, and duration of gelation time. In this work, “thiol-ene” click chemistry was used to quickly and controllably prepare an SS-derived hydrogel to resolve these early concerns. Then, berberine was loaded and used as a model for investigating the drug-release profiles of the prepared hydrogel. The experimental results revealed that this hydrogel is eligible for a long-term release of berberine. Throughout the antibacterial experiments, the released berberine maintained its antibacterial activity. Our work expands the application of SS in biomedical industries in an eco-friendly way. Furthermore, the discussed strategy could provide a reference for the subsequent development of SS-derived materials.

scholarly journals High-resolution three-dimensional probes of biomaterials and their interfaces

2012 ◽  
Vol 370 (1963) ◽  
pp. 1337-1351 ◽  
Author(s):  
Kathryn Grandfield ◽  
Anders Palmquist ◽  
Håkan Engqvist
Keyword(s):  
High Resolution ◽  
Electron Tomography ◽  
Three Dimensional ◽  
Controlled Drug Release ◽  
Biological Tissues ◽  
Dimensional Structure ◽  
Bone Interface ◽  
Biomaterial Interfaces ◽  
Titania Coating

Interfacial relationships between biomaterials and tissues strongly influence the success of implant materials and their long-term functionality. Owing to the inhomogeneity of biological tissues at an interface, in particular bone tissue, two-dimensional images often lack detail on the interfacial morphological complexity. Furthermore, the increasing use of nanotechnology in the design and production of biomaterials demands characterization techniques on a similar length scale. Electron tomography (ET) can meet these challenges by enabling high-resolution three-dimensional imaging of biomaterial interfaces. In this article, we review the fundamentals of ET and highlight its recent applications in probing the three-dimensional structure of bioceramics and their interfaces, with particular focus on the hydroxyapatite–bone interface, titanium dioxide–bone interface and a mesoporous titania coating for controlled drug release.

Hepatocyte Aggregate Culture Technique for Bioreactors in Hybrid Liver Support Systems

1993 ◽  
Vol 16 (12) ◽  
pp. 843-846 ◽  
Author(s):  
J.C. Gerlach ◽  
K. Klöppel ◽  
C. MÜller ◽  
N. Schnoy ◽  
M.D. Smith ◽  
...  
Keyword(s):  
High Performance ◽  
Large Scale ◽  
Support Systems ◽  
Three Dimensional ◽  
Culture Technique ◽  
Dimensional Structure ◽  
Cell Aggregates ◽  
Liver Support ◽  
Aggregate Culture

Utilizing a modified culture technique for hepatocytes, a high performance suspension culture is possible in which hepatocytes spontaneously form cell aggregates. The aggregates of 20-100 cells have been histologically confirmed to hold a three-dimensional structure, they show a long-term external metabolism and a survival time comparable with standard adhesion cultures. This technique has several advantages in the construction of large scale bioreactors for hybrid liver support systems.

Production Methodologies of Meat Analogues: A Review

2021 ◽  
Vol 58 (02) ◽  
pp. 137-148
Author(s):  
J Girija ◽  
S Kamalasundari ◽  
G Hemalatha ◽  
T Umamaheswari
Keyword(s):  
Three Dimensional ◽  
The Body ◽  
Protein Source ◽  
Dimensional Structure ◽  
Manufacturing Processes ◽  
Health Issues ◽  
Good Source ◽  
Meat Protein ◽  
Current Scenario ◽  
Meat Analogue

Meat is a non-vegetarian food and is considered as a good source of quality nutrients. Though meat protein provide the required content of good quality protein for the body, they are also associated with higher cholesterol and fat content, which prove to be a leading cause of serious health issues. This became the primary reason for increase in a shift in demands for plant-based protein source foods. The other reason is environmental impact of animal derived products. Meat analogues are plant-based good quality protein source of food that tastes like meat protein, and texture resemble that of meat. These plant-based meat analogues have some amount of anti-nutrients and allergic compounds, but they can be successfully removed by employing certain processing methods and resemble meat in its functionality properties. This approach of mimicking the plantbased foods to resemble meat involves understanding of the biochemical composition and three-dimensional structure of meat, and replicating those qualities using plant-based ingredients. In the current scenario, the best suitable methods of manufacturing meat analogue are by extrusion and structuring techniques. The meat analogues satisfy the need of meat for both vegetarians and non-vegetarians. This review attempts to outline the different manufacturing processes of meat analogue using plant-based foods, and to analyse the best suitable method.

Degradable Three Dimensional-Printed Polylactic Acid Scaffold with Long-Term Antibacterial Activity

2017 ◽  
Vol 6 (2) ◽  
pp. 2047-2054 ◽  
Author(s):  
Xiaoyuan Li ◽  
Yu Wang ◽  
Min Guo ◽  
Zongliang Wang ◽  
Nannan Shao ◽  
...  
Keyword(s):  
Antibacterial Activity ◽  
Polylactic Acid ◽  
Three Dimensional

scholarly journals Organoids and Colorectal Cancer

Cancers ◽  
2021 ◽  
Vol 13 (11) ◽  
pp. 2657
Author(s):  
Antonio Barbáchano ◽  
Asunción Fernández-Barral ◽  
Pilar Bustamante-Madrid ◽  
Isabel Prieto ◽  
Nuria Rodríguez-Salas ◽  
...  
Keyword(s):  
Colorectal Cancer ◽  
Cell Biology ◽  
Drug Testing ◽  
Current Knowledge ◽  
Three Dimensional ◽  
Subsequent Development ◽  
Intestinal Cell ◽  
Intestinal Epithelial ◽  
Mouse Small Intestine

Organoids were first established as a three-dimensional cell culture system from mouse small intestine. Subsequent development has made organoids a key system to study many human physiological and pathological processes that affect a variety of tissues and organs. In particular, organoids are becoming very useful tools to dissect colorectal cancer (CRC) by allowing the circumvention of classical problems and limitations, such as the impossibility of long-term culture of normal intestinal epithelial cells and the lack of good animal models for CRC. In this review, we describe the features and current knowledge of intestinal organoids and how they are largely contributing to our better understanding of intestinal cell biology and CRC genetics. Moreover, recent data show that organoids are appropriate systems for antitumoral drug testing and for the personalized treatment of CRC patients.

Long-term evolution of the three-dimensional structure of string-fluid complex plasmas in the PK-4 experiment

2021 ◽  
Vol 103 (6) ◽  
Author(s):  
S. Mitic ◽  
M. Y. Pustylnik ◽  
D. Erdle ◽  
A. M. Lipaev ◽  
A. D. Usachev ◽  
...  
Keyword(s):  
Three Dimensional ◽  
Long Term Evolution ◽  
Dimensional Structure ◽  
Three Dimensional Structure ◽  
Term Evolution ◽  
Complex Plasmas

scholarly journals Continued blood cell formation in spherical bodies in a long-term mouse spleen culture

Blood ◽  
1991 ◽  
Vol 77 (6) ◽  
pp. 1211-1217
Author(s):  
Y Matsuya ◽  
N Yanai ◽  
H Ohtani ◽  
H Naganuma ◽  
M Obinata
Keyword(s):  
Blood Cell ◽  
Cell Formation ◽  
Three Dimensional ◽  
Cell Types ◽  
Spherical Body ◽  
The Body ◽  
Dimensional Structure ◽  
Newborn Mice ◽  
Prolonged Culture

During the primary culture of spleen fragments of newborn mice, a spherical body (d = circa 200 to 300 microns) as a three-dimensional cellular organization was formed. Continued production of blood cells from the spherical body was observed without changing its size for about 2 months of culture. Without growth factor, the spherical bodies produced mainly lymphocytes and macrophages. Addition of interleukin-3 enhanced their granulocyte formation, and this enhancement was observed even after a prolonged maintenance without growth factors. The spherical bodies were composed of a uniform mixture of endothelial cells and fibroblasts within the body, and cell-cell contacts between lymphocytes and fibroblasts were notable in the periphery. With prolonged culture, the spherical bodies showed a definite change in their structure by sorting two cell types and the blood cell production gradually decreased. These results suggested that a three-dimensional structure was required for the maintenance, growth, and differentiation of blood cell progenitors in the long-term spleen culture.

Effect of Manganese on the Activity of Antibiotic Against Microorganisms

1970 ◽  
Vol 4 (1) ◽  
Author(s):  
S Rahman ◽  
Pinky Karim ◽  
Abu Asad Chowdhury ◽  
Abul Hasnat
Keyword(s):  
Antimicrobial Activity ◽  
Antibacterial Activity ◽  
Nucleic Acid ◽  
Biological System ◽  
Three Dimensional ◽  
Dimensional Structure ◽  
Optimum Level ◽  
Bacterial Strains ◽  
Key Words Antibiotics ◽  
Life Threatening

Manganese is an important element in biological system. It is essential for enzymatic activity, maintaining three-dimensional structure of protein, for the synthesis of nucleic acid and protein etc. Deficiency of manganese causes different life threatening diseases. Because of this, the optimum level of manganese must be maintained in all biological system. This experiment, therefore, was designed to evaluate the effect of manganese on the activity of different antibiotics (ciprofloxacin, cephradine, amoxicillin, gentamycin, tetracycline, cloxacillin, nalidixic acid, ceftriaxone, metronidazole and carbenicillin) against different microorganisms. It has been observed that antimicrobial activity of an antibiotic increased significantly with concomitant use of manganese salt ranging from 600-6000 ng per antibiotic disc (P < 0.05). It is revealed from the experiment that manganese increases the activity of antibiotic against bacterial strains. Key words: Antibiotics, Manganese, Antibacterial activity. Dhaka Univ. J. Pharm. Sci. Vol.4(1) 2005 The full text is of this article is available at the Dhaka Univ. J. Pharm. Sci. website

scholarly journals NMR resonance assignments of the four isoforms of the hazelnut allergen Cor a 1.04

2019 ◽  
Vol 14 (1) ◽  
pp. 45-49 ◽  
Author(s):  
Sebastian Führer ◽  
Ricarda Zeindl ◽  
Martin Tollinger
Keyword(s):  
Fruits And Vegetables ◽  
Chemical Shifts ◽  
Three Dimensional ◽  
Resonance Assignments ◽  
Subsequent Development ◽  
Dimensional Structure ◽  
Side Chain ◽  
Bet V 1 ◽  
Chemical Shift Data ◽  
Α Helix

Abstract In large parts of Europe, Northern America and China people are suffering from allergies after consuming certain kinds of fruits and vegetables. Typical allergic symptoms range from scratching and itching of the throat to severe symptoms like rhino conjunctivitis and anaphylaxis. For hazelnuts (Corylus avellana), these allergies result from initial sensitization to the birch (Betula verrucosa) pollen allergen Bet v 1 and subsequent development of allergic cross-reactions to proteins that are similar in their three-dimensional structure to the sensitizing protein Bet v 1. The cross-reactive proteins in hazelnut are the four isoforms Cor a 1.04 with a molecular weight of about 17.5 kDa. Significant differences regarding the immunologic behavior of these proteins have been reported. In this work we assigned backbone and side chain 1H, 13C, and 15N chemical shifts of these four isoforms, Cor a 1.0401, Cor a 1.0402, Cor a 1.0403, and Cor a 1.0404 by solution NMR spectroscopy. The chemical shift data confirm the characteristic Bet v onefold for all four isoforms, consisting of seven β-strands that are separated by two short α-helices, along with a long C-terminal α-helix. These data provide the basis for a comparative structural and dynamic analysis of these proteins by NMR in order to characterize their different immunologic cross-reactivities on a molecular level.

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