scholarly journals Sorghum Brown Midrib19 (Bmr19) Gene Links Lignin Biosynthesis to Folate Metabolism

Genes ◽  
2021 ◽  
Vol 12 (5) ◽  
pp. 660
Author(s):  
Adedayo O. Adeyanju ◽  
Scott E. Sattler ◽  
Patrick J. Rich ◽  
Luis A. Rivera-Burgos ◽  
Xiaochen Xu ◽  
...  
Keyword(s):  
Genetic Analysis ◽  
Sorghum Bicolor ◽  
Point Mutation ◽  
Lignin Biosynthesis ◽  
Recurrent Parent ◽  
Brown Midrib ◽  
High Homology ◽  
Tilling Population ◽  
Causal Genes ◽  
Mutant Lines

Genetic analysis of brown midrib sorghum (Sorghum bicolor) mutant lines assembled in our program has previously shown that the mutations fall into four allelic groups, bmr2, bmr6, bmr12 or bmr19. Causal genes for allelic groups bmr2, bmr6 and bmr12, have since been identified. In this report, we provide evidence for the nature of the bmr19 mutation. This was accomplished by introgressing each of the four bmr alleles into nine different genetic backgrounds. Polymorphisms from four resequenced bulks of sorghum introgression lines containing either mutation, relative to those of a resequenced bulk of the nine normal midrib recurrent parent lines, were used to locate their respective causal mutations. The analysis confirmed the previously reported causal mutations for bmr2 and bmr6 but failed in the case of bmr12-bulk due to a mixture of mutant alleles at the locus among members of that mutant bulk. In the bmr19-bulk, a common G → A mutation was found among all members in Sobic.001G535500. This gene encodes a putative folylpolyglutamate synthase with high homology to maize Bm4. The brown midrib phenotype co-segregated with this point mutation in two separate F2 populations. Furthermore, an additional variant allele at this locus obtained from a TILLING population also showed a brown midrib phenotype, confirming this locus as Bmr19.

scholarly journals PENGARUH INOKULAN BAKTERI ASAM LAKTAT DAN ADITIF TERHADAP KUALITAS DAN KARAKTERISTIK SILASE SORGUM MUTAN 2 1 BROWN MIDRIB (Sorghum bicolor L. Moench)

Pastura ◽  
2019 ◽  
Vol 9 (1) ◽  
pp. 40
Author(s):  
R. Sriagtula ◽  
I. Martaguri ◽  
J. Hellyward ◽  
S. Sowmen
Keyword(s):  
Sorghum Bicolor ◽  
Brown Midrib ◽  
Duncan Multiple Range Test ◽  
Sorghum Bicolor L ◽  
Range Test

Penelitian ini bertujuan untuk mengobservasi pengaruh penambahan inokulasi bakteri asam laktat (BAL) dan aditif terhadap kualitas dan karakterietik silase whole crop sorgum mutan brown midrib (Sorghum bicolor L. Moench) galur Patir 3.7 yang dipanen pada fase soft dough. Penelitian dilaksanakan secara eksperimen menggunakan rancangan acak lengkap pola faktorial dengan 4 ulangan. Faktor A yaitu A1 = tanpa BAL, A2= penambahan BAL. Faktor B terdiri dari B1= tanpa aditif, B2= dedak, B3= jagung. Sumber BAL yang digunakan berasal dari inokulan komersil dari minuman fermentasi merk Yakult dengan dosis 1 ml (v/w) atau 11×109 CFU/ml/berat segar. Aditif terdiri dari dedak padi dan jagung halus digunakan sebanyak 3% (g/g)/berat segar. Parameter yang diamati adalah karakteristik dan kualitas silase meliputi nilai pH, nilai fleigh (NF), kandungan bahan kering (BK), protein kasar (PK), serat kasar (SK), lemak kasar (LK) dan Abu. Data dianalisis berdasarkan analisis keragaman menurut Duncan Multiple Range Test (DMRT). Hasil penelitian menunjukkan bahwa tidak terdapat interaksi (P>0,05) antara penambahan BAL dan aditif terhadap pH, NF, BK, PK, SK, LK dan abu, sedangkan faktor tunggal adititif memberikan pengaruh berbeda nyata (P<0,05) lebih tinggi terhadap kandungan BK silase whole crop sorgum mutan BMR. Dari penelitian ini dapat disimpulkan bahwa secara umum penambahan inokulan BAL dan aditif menghasilkan karakteristik dan kualitas silase yang sama, namun demikian penambahan dedak padi dan jagung halus menghasilkan BK silase yang lebih tinggi dibanding tanpa BAL dan aditif. Kata kunci: aditif, BAL, brown midrib, silase, sorgum

scholarly journals Genetic analysis of the Arabidopsis TIR1/AFB auxin receptors reveals both overlapping and specialized functions

eLife ◽  
2020 ◽  
Vol 9 ◽  
Author(s):  
Michael J Prigge ◽  
Matthieu Platre ◽  
Nikita Kadakia ◽  
Yi Zhang ◽  
Kathleen Greenham ◽  
...  
Keyword(s):  
Genetic Analysis ◽  
Early Embryo ◽  
Wild Type ◽  
The Family ◽  
Dependent Inhibition ◽  
Root Gravitropism ◽  
Specialized Function ◽  
Mutant Lines

The TIR1/AFB auxin co-receptors mediate diverse responses to the plant hormone auxin. The Arabidopsis genome encodes six TIR1/AFB proteins representing three of the four clades that were established prior to angiosperm radiation. To determine the role of these proteins in plant development we performed an extensive genetic analysis involving the generation and characterization of all possible multiply-mutant lines. We find that loss of all six TIR1/AFB proteins results in early embryo defects and eventually seed abortion, and yet a single wild-type allele of TIR1 or AFB2 is sufficient to support growth throughout development. Our analysis reveals extensive functional overlap between even the most distantly related TIR1/AFB genes except for AFB1. Surprisingly, AFB1 has a specialized function in rapid auxin-dependent inhibition of root growth and early phase of root gravitropism. This activity may be related to a difference in subcellular localization compared to the other members of the family.

scholarly journals GENETIC ANALYSIS OF THE TYPES OF DEVELOPMENT OF MUTANT LINES FROM COMMON WHEAT VARIETIES

REPORTS ◽  
2020 ◽  
Vol 6 (334) ◽  
pp. 22-28
Author(s):  
N. Sarybay ◽  
◽  
Zh. Zh. Chunetova ◽  
D. M. Iskakova ◽  
B.A. Zhumabaeva ◽  
...  
Keyword(s):  
Cell Division ◽  
Genetic Analysis ◽  
Zinc Chloride ◽  
Cadmium Chloride ◽  
Stem Elongation ◽  
Morphological Variability ◽  
Main Task ◽  
Wheat Varieties ◽  
Mutant Lines ◽  
Cadmium Salt

Among cereals, a special place is occupied by wheat as the main food of humanity and in improving the country's economy. The main task of breeding is to produce valuable wheat varieties that are stable to the unfavorable external environment of various natural climatic zones of Kazakhstan, consisting of a combination of valuable features. The prospects for the use of mutants in the process of hybridization are reflected in the achievements of world and domestic selection. The effect of various solutions of chemical compounds on the plant was observed from the first days during the growth of wheat sprouts. The growth of the first sprout of seeds treated with a certain solution of cadmium chloride and zinc chloride - 0.01%, the activity of cell division and structural disorders of chromosomes were manifested by variability in the varieties Kazakhstani3, Shagala, Zhenis and Lutescens 32. When treating dry wheat grain with a concentration of 0.1% cadmium chloride, it brought the growth of the Sprout to a lethal state, 0.01% inhibited the growth of the Sprout for 4 days, and in a 0.001% solution, the growth rate of the control grains was the same. On the contrary, a 0.1% vertical solution of zinc chloride inhibited the growth of the Sprout for 1 Week, 0.01% for three days. Among the studied concentrations, a 0.01% solution of cadmium chloride inhibited the growth of sprouts, causing chromosomal aberrations and morphological variability in cell division. Therefore, a concentration of 0.01% of cadmium salt was obtained as an optimal concentration to expand the limits of variability in wheat. In this regard, in our study, it was found that a 0.01% solution of cadmium chloride is an effective concentration that expands the limits of variability in wheat. The effect of this amount of solution leads to morphological changes in the plant (Binding of the stem, elongation of the earlobe, increase in the number and weight of grains in the headlobe, increase in the weight of 1000 grains, etc.), and changes in the characteristics of the M1 - M4 offspring are constantly inherited. Mutant lines L1, L2 and L3 differ in valuable breeding characteristics under the influence of a 0.01 percent solution of cadmium salt were obtained for the Kazakhstanskaya 3 and Shagala varieties. A genetic analysis of the type of development of these altered lines was carried out, as a result of which a long (16 cm) dense ear (0.80) changed from the Gull variety to a prismatic plant of the control variety (0.50), as a result of hybridization, its summer type (from the vrn gene) to the autumn type (Vrn gene). As a result of the study, Kazakhstanskaya 3, Kazakhstanskaya mutant 3 varieties contain the genes Vrn 1, Vrn 3. Well, it was found that Shagala varieties consist of the genes Vrnl, Vrn2. Varieties containing the genes Vrn1, Vrn3 show rapid maturation. However, they differed from each other in the period of intoxication.

IKZF1 Deletion Is Strongly Associated with Risk of Relapse in Intermediate Risk Group in JACLS ALL02 Cohort

Blood ◽  
2011 ◽  
Vol 118 (21) ◽  
pp. 1455-1455 ◽  
Author(s):  
Daisuke Asai ◽  
Toshihiko Imamura ◽  
Souichi Suenobu ◽  
Daiichiro Hasegawa ◽  
Takao Deguchi ◽  
...  
Keyword(s):  
Genetic Analysis ◽  
Point Mutation ◽  
Peripheral Blood ◽  
Risk Group ◽  
Current Therapy ◽  
Intermediate Risk ◽  
Rt Pcr ◽  
Over Expression ◽  
Cell Counts ◽  
Risk Of Relapse

Abstract Abstract 1455 Introduction: Despite the progress of the current therapy, approximately 20 % of pediatric patients with B-cell-presursor (BCP)-ALL experience relapse who had no conventional adverse prognostic factor. Recently, alteration of the IKZF1 gene has been reported to be associated with a poor outcome in pediatric BCP-ALL without BCR-ABL. In addition, it is also reported that a significant proportion of these cases with the alteration of IKZF1 shared the point mutation of JAK2 and over-expression of cytokine receptor-like factor 2 (CRLF2). Herein, in order to assess the prognostic value of these genetic abnormalities in Japanese cohort, we conducted genetic analysis of IKZF1, JAK2 and CRLF2 in pediatric BCP-ALL. Materials and Methods: Diagnostic bone marrow or peripheral blood samples of 215 pediatric BCP-ALL patients treated according to Japan Association of Childhood Leukemia Study (JACLS) ALL02 protocol from April, 2002 to May, 2008 were examined in this study. All patients were categorized into high risk (HR) group defined as follows;(1) initial white blood cell counts more than 10,000 /μl, (2) age at diagnosis is > 10 years and (3) good response to initial predonisolone (PSL) treatment ( blast counts in peripheral blood is less than 1,000 /μl after one week PSL treatment). Ph+ALL and infantile ALL patients were excluded in this protocol. The patients with Down syndrome were also excluded in this genetic analysis. The deletion of IKZF1 was determined using multiplex ligation-dependent probe amplification (MLPA) in 212 patients whose diagnostic DNA samples were available. The expression of isoform of IKZF1 was determined by RT-PCR in 113 patients whose diagnostic RNA samples were available. The expression level of CRLF2 was also determined by real time RT-PCR in 112 patients and over-expression was defined as over ten times more than median expression value. The presence of P2RY8-CRLF2 fusion was examined by RT-PCR or MLPA in the patients with CRLF2 over-expression or IKZF1 deletion. JAK2 mutations were also determined by direct sequencing of the exon 16, 20 and 21 in the patients with IKZF1 deletion. Results: The deletion of IKZF1 gene was present in 19 of 212 (9.0 %) patients. In detail, the mono-allelic deletion of entire IKZF1 gene was present in 10 of 19 patients. On the other hand, the expression of dominant-negative IK6 isoform was present in 9 of 112 (8.0%) patients including 12 patients with IKZF1 deletion. The expression of IK6 was present in 4 of 12 (33.3%) IKZF1 deleted patients. Interestingly, 5 of 9 patients with IK6 expression had no alteration of IKZF1 gene. In terms of CRLF2, over-expression was detected in 16 of 112 patients (14.3 %). However, P2RY8-CRLF2 fusion was not detected in these 16 patients with altered CRLF2 expression. Strikingly, none of the patients with IKZF1 deletion (n=19) had either P2RY8-CRLF2 fusion or JAK2 exon 16, 20 and 21 mutation. Patients with IKZF1 gene deletion had significantly worse relapse rate than those without IKZF1 deletion (7/19 vs 22/193, p<0.01). On the contrary, none of the patients with IK6 expression experienced relapse. Discussions: This study confirmed that the presence of IKZF1 deletion was strongly correlated with risk of relapse in intermediate risk group in JACLS ALL02 cohort. Thus, we expect that IKZF1 deletion is an independent predictor of treatment outcome and represents a candidate of prognostic marker to be integrated in future algorithms for early risk stratification in pediatric BCP-ALL. Strikingly, point mutation of JAK2 exon 16, 20 and 21 or P2RY8 -CRLF2 fusion was rarely present even in BCP-ALL patients with IKZF1 deletion. There might be unrevealed class I mutation cooperating with IKZF1 alteration in Japanese BCP-ALL cohort. Disclosures: No relevant conflicts of interest to declare.

A series of novel mutants of Arabidopsis thaliana that are defective in the formation of continuous vascular network: calling the auxin signal flow canalization hypothesis into question

Development ◽  
2000 ◽  
Vol 127 (15) ◽  
pp. 3197-3204 ◽  
Author(s):  
K. Koizumi ◽  
M. Sugiyama ◽  
H. Fukuda
Keyword(s):  
Arabidopsis Thaliana ◽  
Pattern Formation ◽  
Genetic Analysis ◽  
Molecular Mechanisms ◽  
Vascular Tissue ◽  
Early Stage ◽  
Diffusion Reaction ◽  
Signal Flow ◽  
Vein Formation ◽  
Mutant Lines

For the genetic analysis of molecular mechanisms underlying temporal and spatial regulation of vascular pattern formation, we isolated mutants of Arabidopsis thaliana that are impaired in vascular patterning. Microscopic examination of the cotyledonary venation of 3,400 M(3) lines led to the identification of 12 mutant lines. Genetic analysis of 8 of these mutant lines indicated that vein pattern formation in these lines resulted from monogenic recessive mutations in 7 different genes, designated VAN1 through VAN7. Mutations in VAN1 through VAN6 genes caused fragmentation (disconnection or partial loss) of lateral veins of the cotyledon and tertiary veins of the rosette leaf whereas they were less injurious to the formation of major veins. Detailed characterization of the van3 mutant using pAthb8::GUS and pTED3::GUS, as molecular markers for the early stage of vascular tissue formation showed that the provascular tissue of the cotyledonary lateral veins was differentiated in fragments during late embryogenesis. These phenotypes of the van mutants are discussed in relation to the auxin signal flow canalization hypothesis and the diffusion-reaction prepattern hypothesis, with the fragility of the continuity in the minor vein formation favoring the latter hypothesis.

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