scholarly journals Association of Indolebutyric Acid with Azospirillum brasilense in the Rooting of Herbaceous Blueberry Cuttings

Horticulturae ◽  
2019 ◽  
Vol 5 (4) ◽  
pp. 68 ◽  
Author(s):  
Koyama ◽  
Aparecido Ribeiro Júnior ◽  
Mariani Zeffa ◽  
Tadeu Faria ◽  
Mitsuharu Saito ◽  
...  
Keyword(s):  
Root Length ◽  
Azospirillum Brasilense ◽  
Dry Mass ◽  
Plant Growth Promoting Bacteria ◽  
Indolebutyric Acid ◽  
Rooted Cuttings ◽  
Plant Growth Promoting ◽  
Rooting Of Cuttings ◽  
Mist Chamber ◽  
Root Growth And Development

Association between auxins and plant growth-promoting bacteria can stimulate root growth and development of fruit crop nursery plants, and can be a promising biological alternative to increase the rooting of cuttings. The objective of this study was to assess the viability of producing ‘Powderblue’ blueberry nursery plants from cuttings using different doses of indolebutyric acid (IBA) in association with Azospirillum brasilense. The following treatments were tested: 0 (control); 500 mg L−1 of IBA; 1000 mg L−1 of IBA; A. brasilense; 500 mg L−1 of IBA + A. brasilense; and 1000 mg L−1 of IBA + A. brasilense. The experimental design was completely randomized, with six treatments and four replicates, and each plot (box) consisted of 10 cuttings. The boxes were arranged in a mist chamber with an intermittent regimen controlled by a timer and solenoid valve. After 90 days, the following variables were assessed: rooted cuttings; survival of cuttings; foliar retention; sprouting; cuttings with callus; root dry mass per cutting; number of roots per cutting; and root length. It was observed that the application of IBA with the A. brasilense rhizobacteria increased the number of roots of ‘Powderblue’ blueberry cuttings, while the treatments with IBA alone and IBA 1000 mg L−1 + A. brasilense increased the root length of cuttings. However, treatments with IBA and A. brasilense had no impact on % rooted cuttings and % survival of cuttings.

The effect of native and ACC deaminase-containing Azospirillum brasilense Cd1843 on the rooting of carnation cuttings

2005 ◽  
Vol 51 (6) ◽  
pp. 511-514 ◽  
Author(s):  
Qiaosi Li ◽  
Saleema Saleh-Lakha ◽  
Bernard R Glick
Keyword(s):  
Plant Growth ◽  
Azospirillum Brasilense ◽  
Indoleacetic Acid ◽  
Acc Deaminase ◽  
Plant Growth Promoting Bacteria ◽  
Indolebutyric Acid ◽  
Proposed Model ◽  
Plant Growth Promoting ◽  
Growth Promoting

Carnation cuttings treated with non-transformed and 1-aminocyclopropane (ACC) deaminase-containing Azospirillum brasilense Cd1843 produced significantly more roots than untreated controls and fewer roots than cuttings treated with 0.1% indolebutyric acid (IBA). The roots produced by cuttings treated with ACC deaminase-containing Azospirillum brasilense Cd1843 were the longest roots resulting from any of the treatments, followed by non-transformed Azospirillum brasilense Cd1843, 0.1% IBA, and treatment with water. The results are interpreted in terms of a previously proposed model of bacterial promotion of plant growth by ACC deaminase and indoleacetic acid, and may have implications for the use of plant growth-promoting bacteria in the flower industry.Key words: ACC deaminase, carnation, cuttings, rooting, Azospirillum brasilense.

scholarly journals Rooting of herbaceous cuttings of Malpighia emarginata D.C. (CAMB-06 and APU-04 selections) associated with the use of indolebutyric acid and liquid extract of Cyperus rotundus L.

2020 ◽  
Vol 42 (1) ◽  
Author(s):  
Débora Thaís Mühlbeier ◽  
Renata Koyama ◽  
Osmar Jose Chaves Junior ◽  
Ibrar Hussain ◽  
Neusa Maria Colauto Stenzel ◽  
...  
Keyword(s):  
Experimental Design ◽  
Aqueous Extract ◽  
Dry Mass ◽  
Cyperus Rotundus ◽  
Indolebutyric Acid ◽  
Rooted Cuttings ◽  
Liquid Extract ◽  
Malpighia Emarginata ◽  
Cutting Length ◽  
Rooting Of Cuttings

Abstract The objective of this work was to evaluate the rooting of herbaceous cuttings of CAMB-06 and APU-04 acerola cherry selections associated with the use of indolebutyric acid (IBA) and a concentration of aqueous extract of coco-grass. Cuttings were treated by immersion with different concentrations of IBA (0; 1,000; 2,000; 3,000; 4,000 mg L-1) and one application of aqueous extract of coco-grass in proportion 100% and placed to root in plastic boxes containing vermiculite. The experimental design was a completely randomized in a 2×6 factorial arrangement (two acerola cherry selections and six solutions for rooting) with four replicates of ten cuttings. After 90 days of cutting, the following variables were evaluated: cutting survival (%); leaf retention (%); rooted cuttings (%); sprouting (%); number of roots per cutting (%); length of roots (cm) and dry mass of roots per cutting (g). It was concluded that the application of IBA at 4,000 mg L-1 presented a better root development, resulting in a higher percentage of rooted cuttings and higher number and dry mass of roots in relation to IBA at 0 mg L-1 for CAMB-06 selection, while the APU-04 selection can be efficiently multiplied by herbaceous cuttings without the use of IBA. Under the conditions that the trials was conducted, the application of 100% aqueous extract of coco-grass was not effective in promoting the rooting of cuttings of CAMB-06 and APU-04 acerola cherry selections.

Isolation, identification and characterization of native plant growth promoting bacteria and their plant growth promotion in Zea mays

2021 ◽  
Vol 16 (8) ◽  
pp. 75-80
Author(s):  
Pitchaiah Pelapudi ◽  
Sasikala Ch ◽  
Swarnabala Ganti
Keyword(s):  
Plant Growth ◽  
Plant Growth Promotion ◽  
Root Length ◽  
Growth Promotion ◽  
Shoot Length ◽  
Root Weight ◽  
Plant Growth Promoting Bacteria ◽  
Plant Growth Promoting ◽  
Growth Promoting ◽  
Shoot Weight

In the present rapid growing world, need for a sustainable agricultural practice which helps in meeting the adequate food demand is much needed. In this context, plant growth promoting bacteria were brought into the spot light by the researchers. Though the plant growth promoting bacteria have several beneficial applications, due to some of the disadvantages in the field conditions, they lagged behind. In the current research work, native PGPR were isolated from the rhizosphere soil samples of maize with an aim to isolate the nitrogen fixing, phosphate solubilising and potash solubilising bacteria. Out of the several isolates, potent PGPR isolates viz., Paenibacillus durus PCPB067, Bacillus megaterium PCBMG041 and Paenibacillus glucanolyticus PCPG051 were isolated and identified by using the 16 S rRNA gene sequencing studies. Genomic DNA sequences obtained were deposited in the NCBI Genbank and accession numbers were assigned as MW793452, MW793456 and MW843633. In order to check the efficacy of the PGPR isolates, pot trials were conducted by taking maize as the host plant. Several parameters viz. shoot length, shoot weight, root length, root weight and weight of the seeds were tested in which PGP treatment showed good results (shoot length - 187±3.5 cm, shoot weight - 31±4 g, root length - 32±3.6 cm, root weight - 17±2 g, yield- 103.3±6.1 g) when compared to the chemical fertilizer treatment (shoot length - 177±3.5 cm, shoot weight - 25±3.6 g, root length - 24±3.5 cm, root weight - 14.6±1.52 g, yield- 85.6±7.6 g). Based on the results, it can be stated that these native PGPR isolates can be effectively used in the plant growth promotion of maize.

A Vegetative Propagation System for Tamarack

1986 ◽  
Vol 3 (3) ◽  
pp. 91-93 ◽  
Author(s):  
Robert E. Farmer ◽  
Heather A. Foster ◽  
Olenka Bakowsky ◽  
Brian MacDonald ◽  
Gwenoth O'Reilly ◽  
...  
Keyword(s):  
Vegetative Propagation ◽  
Shoot Growth ◽  
Indolebutyric Acid ◽  
Rooted Cuttings ◽  
Normal Shoot ◽  
Rooting Of Cuttings ◽  
Natural Stands ◽  
North Western

Abstract Greenwood cuttings from greenhouse-produced tamarack seedlings and 3- to 10-year-old tamarack wildlings transplanted from natural stands in north-western Ontario were rooted under mist in peat-vermiculite. Eighty-five percent of cuttings from seedlings rooted; treatment with indolebutyric acid increased number of roots per cutting, but not rooting percent. At 6 weeks after planting, rooting of cuttings from wildlings averaged 66%, and at 12 weeks, 91%. Twelve-week rooting percent of cuttings from individual ortets ranged from 12 to 100, but cuttings from the majority of ortets exhibited 100% rooting. Nearly all rooted cuttings survived overwintering outdoors and initiated normal shoot growth after forcing in mid-winter. The described propagation system is recommended for production of container stock for tamarack plantations. North. J. Appl. For. 3:91-93, Sept. 1986.

scholarly journals Protection of Tomato Seedlings against Infection by Pseudomonas syringae pv. Tomato by Using the Plant Growth-Promoting Bacterium Azospirillum brasilense

2002 ◽  
Vol 68 (6) ◽  
pp. 2637-2643 ◽  
Author(s):  
Yoav Bashan ◽  
Luz E. de-Bashan
Keyword(s):  
Plant Growth ◽  
Pseudomonas Syringae ◽  
Azospirillum Brasilense ◽  
Dry Weight ◽  
Tomato Plants ◽  
Bacterial Speck ◽  
Plant Growth Promoting ◽  
Bacterial Speck Disease ◽  
Growth Promoting ◽  
Mist Chamber

ABSTRACT Pseudomonas syringae pv. tomato, the causal agent of bacterial speck of tomato, and the plant growth-promoting bacterium Azospirillum brasilense were inoculated onto tomato plants, either alone, as a mixed culture, or consecutively. The population dynamics in the rhizosphere and foliage, the development of bacterial speck disease, and their effects on plant growth were monitored. When inoculated onto separate plants, the A. brasilense population in the rhizosphere of tomato plants was 2 orders of magnitude greater than the population of P. syringae pv. tomato (107 versus 105 CFU/g [dry weight] of root). Under mist chamber conditions, the leaf population of P. syringae pv. tomato was 1 order of magnitude greater than that of A. brasilense (107 versus 106 CFU/g [dry weight] of leaf). Inoculation of seeds with a mixed culture of the two bacterial strains resulted in a reduction of the pathogen population in the rhizosphere, an increase in the A. brasilense population, the prevention of bacterial speck disease development, and improved plant growth. Inoculation of leaves with the mixed bacterial culture under mist conditions significantly reduced the P. syringae pv. tomato population and significantly decreased disease severity. Challenge with P. syringae pv. tomato after A. brasilense was established in the leaves further reduced both the population of P. syringae pv. tomato and disease severity and significantly enhanced plant development. Both bacteria maintained a large population in the rhizosphere for 45 days when each was inoculated separately onto tomato seeds (105 to 106 CFU/g [dry weight] of root). However, P. syringae pv. tomato did not survive in the rhizosphere in the presence of A. brasilense. Foliar inoculation of A. brasilense after P. syringae pv. tomato was established on the leaves did not alleviate bacterial speck disease, and A. brasilense did not survive well in the phyllosphere under these conditions, even in a mist chamber. Several applications of a low concentration of buffered malic acid significantly enhanced the leaf population of A. brasilense (>108 CFU/g [dry weight] of leaf), decreased the population of P. syringae pv. tomato to almost undetectable levels, almost eliminated disease development, and improved plant growth to the level of uninoculated healthy control plants. Based on our results, we propose that A. brasilense be used in prevention programs to combat the foliar bacterial speck disease caused by P. syringae pv. tomato.

scholarly journals Compatibility of Azospirillum brasilense with Pesticides Used for Treatment of Maize Seeds

2020 ◽  
Vol 2020 ◽  
pp. 1-8
Author(s):  
Mariana S. Santos ◽  
Artur B. L. Rondina ◽  
Marco A. Nogueira ◽  
Mariangela Hungria
Keyword(s):  
Plant Growth ◽  
Root Hair ◽  
Azospirillum Brasilense ◽  
Plant Protection ◽  
Plant Growth Promoting Bacteria ◽  
Root Volume ◽  
Maize Seeds ◽  
Plant Growth Promoting ◽  
Growth Promoting

Seed treatment with chemical pesticides is commonly used as an initial plant protection procedure against pests and diseases. However, the use of such chemicals may impair the survival and performance of beneficial microorganisms introduced via inoculants, such as the plant growth-promoting bacterium Azospirillum brasilense. We assessed the compatibility between the most common pesticide used in Brazil for the treatment of maize seeds, composed of two fungicides, and one insecticide, with the commercial strains Ab-V5 and Ab-V6 of A. brasilense, and evaluated the impacts on initial plant development. The toxicity of the pesticide to A. brasilense was confirmed, with an increase in cell mortality after only 24 hours of exposure in vitro. Seed germination and seedling growth were not affected neither by the A. brasilense nor by the pesticide. However, under greenhouse conditions, the pesticide affected root volume and dry weight and root-hair incidence, but the toxicity was alleviated by the inoculation with A. brasilense for the root volume and root-hair incidence parameters. In maize seeds inoculated with A. brasilense, the pesticide negatively affected the number of branches, root-hair incidence, and root-hair length. Therefore, new inoculant formulations with cell protectors and the development of compatible pesticides should be searched to guarantee the benefits of inoculation with plant growth-promoting bacteria.

scholarly journals Quantification of Azospirillum brasilense FP2 Bacteria in Wheat Roots by Strain-Specific Quantitative PCR

2015 ◽  
Vol 81 (19) ◽  
pp. 6700-6709 ◽  
Author(s):  
Maria Isabel Stets ◽  
Sylvia Maria Campbell Alqueres ◽  
Emanuel Maltempi Souza ◽  
Fábio de Oliveira Pedrosa ◽  
Michael Schmid ◽  
...  
Keyword(s):  
Plant Growth ◽  
Quantitative Pcr ◽  
Dna Sequences ◽  
Azospirillum Brasilense ◽  
Plant Growth Promoting Bacteria ◽  
Genome Sequences ◽  
Wheat Roots ◽  
Content Type ◽  
Plant Growth Promoting ◽  
Growth Promoting

ABSTRACTAzospirillumis a rhizobacterial genus containing plant growth-promoting species associated with different crops worldwide.Azospirillum brasilensestrains exhibit a growth-promoting effect by means of phytohormone production and possibly by N2fixation. However, one of the most important factors for achieving an increase in crop yield by plant growth-promoting rhizobacteria is the survival of the inoculant in the rhizosphere, which is not always achieved. The objective of this study was to develop quantitative PCR protocols for the strain-specific quantification ofA. brasilenseFP2. A novel approach was applied to identify strain-specific DNA sequences based on a comparison of the genomic sequences within the same species. The draft genome sequences ofA. brasilenseFP2 and Sp245 were aligned, and FP2-specific regions were filtered and checked for other possible matches in public databases. Strain-specific regions were then selected to design and evaluate strain-specific primer pairs. The primer pairs AzoR2.1, AzoR2.2, AzoR5.1, AzoR5.2, and AzoR5.3 were specific for theA. brasilenseFP2 strain. These primer pairs were used to monitor quantitatively the population ofA. brasilensein wheat roots under sterile and nonsterile growth conditions. In addition, coinoculations with other plant growth-promoting bacteria in wheat were performed under nonsterile conditions. The results showed thatA. brasilenseFP2 inoculated into wheat roots is highly competitive and achieves high cell numbers (∼107CFU/g [fresh weight] of root) in the rhizosphere even under nonsterile conditions and when coinoculated with other rhizobacteria, maintaining the population at rather stable levels for at least up to 13 days after inoculation. The strategy used here can be applied to other organisms whose genome sequences are available.

scholarly journals Initial performance of maize in response to NPK fertilization combined with Herbaspirillum seropedicae

Revista CERES ◽  
2012 ◽  
Vol 59 (6) ◽  
pp. 841-849 ◽  
Author(s):  
Marihus Altoé Baldotto ◽  
Lílian Estrela Borges Baldotto ◽  
Rogério Batista Santana ◽  
Cláudio Roberto Marciano
Keyword(s):  
Dry Mass ◽  
Plant Growth Promoting Bacteria ◽  
Initial Growth ◽  
Herbaspirillum Seropedicae ◽  
Biotechnological Approach ◽  
Plant Growth Promoting ◽  
Maize Plants ◽  
Growth Promoting ◽  
Treatment Application ◽  
Npk Fertilization

The inoculation with plant growth-promoting bacteria can be a technological approach useful for increasing the production of maize. The objective of this study was to evaluate the initial performance of maize in response to application of doses of NPK combined with the inoculation of the diazotrophic bacteria Herbaspirillum seropedicae in an greenhouse experiment. The experiment consisted of six fertilizer levels: 0, 25, 50, 75, 100 and 200% of the recommended dose of NPK applied to maize inoculated and non-inoculated with H. seropedicae. At 30 days after the treatment application, the growth characteristics and nutritional status of the plants were evaluated. Plant development was influenced by fertilization, but it was enhanced by combination with the bacteria, which resulted in significant increases in the dry mass of shoots (7%) and leaf area (9%) when compared with non-inoculated plants. The results showed increases in the concentration of N (11%), P (30%) and K (17%) of maize plants in response to bacterial inoculation together with NPK compared with plants that were applied fertilize only. The greater consistency and stability response of the host plant to bacterization in the presence of chemical fertilizer indicate a promissory biotechnological approach for improving the initial growth and adaptation of maize to the cultivation environment.

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