scholarly journals Chemical Composition and Anti-Microbial Activity of Hog Plum (Spondias mombin L.) Peel Oil Extracted from Different Regions of Tropical Climates

Horticulturae ◽  
2021 ◽  
Vol 7 (11) ◽  
pp. 428
Author(s):  
Md. Emrul Ahsan Plabon ◽  
Shakti Chandra Mondal ◽  
Md. Mamun Or Rashid ◽  
M. Kaium Chowdhury ◽  
Abu Saeid ◽  
...  

The hydro-distilled essential oil of hog plum peel may be used for enhancing the flavor and taste of food products as well as for hiding the unpleasant odor of drugs. Thus, the waste peels of Spondias mombin appear to have economic importance. To find out the chemical composition and anti-microbial properties of hog plum peel oil, the samples were collected from different regions of Bangladesh for extraction and identification of volatile compounds by GC-MS, where dichloromethane was used as an extraction solvent. The required standard analytical methods were used to assay the anti-microbial properties of hog plums. In this study, pentenyl-3-thy-met-4-alpha-methyl-alpha-ethanol-oxiranen (29.04%), (3,3.1,1)-4-dimethylethyl-1,1-phenol (8.00%), cycohexanol-3 (10.85%), 4-hydroxy-penzeneethanamine (7.09%), hydroxylamine (4.63%), dibutyl phthalate (6.85%), etc., were majorly determined. Consequently, the highest content of 75.81% volatile compounds was found in the Dinajpur district, where the lowest content of 35.00% was found in the Rajshahi district. In contrast, 33 volatile compounds were identified in hog plum peels collected from the Barishal district, whereas 22 compounds were detected in the peel samples collected from the Dinajpur district. In addition, the antimicrobial activity of the oil was analyzed by the disk diffusion method, and the results revealed that the highest Ciprocin content was recorded in the hog plums of Barishal (22.0–23.0 mm), while the lowest was recorded in the Mymensingh sample (20.67–21.63 mm), which was on par with Rajshahi sample (20.70–21.50 mm). The results of the anti-fungal activities of the peel oil showed the highest zone of inhibition against the Aspergillus niger (11.63 ± 0.0003 mm) and Penicillium oxalicum (13.67 ± 1.97 mm) content of the Rajshahi and Pabna district samples, respectively.

2012 ◽  
Vol 7 (5) ◽  
pp. 1934578X1200700 ◽  
Author(s):  
Alexis Peña ◽  
Luis Rojas ◽  
Rosa Aparicio ◽  
Libia Alarcón ◽  
José Gregorio Baptista ◽  
...  

The essential oil of the leaves of Espeletia nana Cuatrec, obtained by hydrodistillation, was analyzed by GC-MS, which allowed the identification of 24 components, which made up 99.9% of the oil. The most abundant compounds were α-pinene (38.1%), β-pinene (17.2%), myrcene (15.0%), spathulenol (4.2%), bicyclogermacrene (4.0%), α-zingiberene (4.0%), and γhimachalene (3.7%). Antibacterial activity was tested against Gram-positive and Gram-negative bacteria using the agar disk diffusion method. Activity was observed only against Gram-positive bacteria. MIC values were determined for Staphylococcus aureus ATCC 25923(200 μg/mL) and Enterococcus faecalis ATCC 29212 (600 μg/mL).


2020 ◽  
Vol 48 (1) ◽  
pp. 53-65
Author(s):  
Tatjana D. GOLUBOVIĆ ◽  
Gordana S. STOJANOVIĆ ◽  
Dušanka V. KITIĆ ◽  
Bojan K. ZLATKOVIĆ ◽  
Dragana R. PAVLOVIĆ ◽  
...  

The ethanol extracts of selected Acinos Miller species were investigated in terms of chemical composition, antimicrobial and antioxidative activities. Qualitative and quantitative analyses of the extracts were performed using GC and GC-MS. Eighty-six constituents, accounting for 93.70-99.99% of the total composition of the extracts, were identified. The ethanol extracts of A. majoranifolius, A. suaveolens and A. alpinus were characterized by domination of monoterpenes, representing 85.03%, 57.39% and 28.02% of the total extracts, respectively. Fatty acids and their esters in the A. arvensis and A. graveolens extracts reached 28.97% and 30.75%. Also, n-alkanes were the major compounds found in A. hungaricus ethanol extract (30.98%). The extracts were characterized by determination of total polyphenols, flavonoids and tannins. Besides, the antioxidant activity of the investigated extracts was estimated by two assays: DPPH and FRAP test. The highest antioxidant activity was observed in the extract of A. alpinus which had high levels of all examined polyphenol classes. A disk diffusion method was employed for the determination of the antimicrobial activities of the ethanol extracts. Gram-positive: Bacillus subtilis, Sarcina lutea, Staphylococcus aureus, Clostridium pyogenes, Enterococcus sp., Micrococcus flavus; Gram-negative: Klebsiella pneumoniae, Pseudomonas aerugionsa, Salmonella enteritidis, Proteus vulgaris, Escherichia coli and fungal organisms: Aspergillus niger, Candida albicans and Saccharomyces cerevisiae were used as test microorganisms. The results of preliminary bioassay demonstrated that the A. alpinus ethanol extract could be a possible source of compounds with antioxidant and antimicrobial activity.


2015 ◽  
Vol 43 (2) ◽  
pp. 327-334 ◽  
Author(s):  
Mihaela NICULAE ◽  
Laura STAN ◽  
Emoke PALL ◽  
Anamaria Ioana PAȘTIU ◽  
Iulia Maria BALACI ◽  
...  

The study was aimed to characterize the chemical composition and the antimicrobial activity of Romanian propolis ethanolic extracts (EEP) against antibiotic-sensitive and antibiotic-resistant E. coli strains isolated from bovine mastitis. The preliminary antimicrobial screening was performed by a disk diffusion method, followed by determination of minimum inhibitory concentrations (MIC) and minimum bactericidal concentrations (MBC) based on broth microdilution assay; further, the synergistic action of propolis with antimicrobial drugs was assessed by a disk diffusion method on agar containing subinhibitory concentrations of propolis. For the chemical characterisation of EEP, the flavonoids (flavones/flavonols, flavanones/dihydroflavonols) and total phenolics were evaluated by spectrophotometric methods. The phenolic compounds of these extracts were also determined using HPLC. The results indicated for Romanian propolis ethanolic extracts the typical poplar composition profile with flavonoids and phenolic acids as main biological active compounds, with chromatographic analysis data confirmed also spectrophotometrically. In addition, positively correlated with the chemical composition, a strong antimicrobial efficacy was exhibited towards E. coli strains, along with interesting synergistic interaction with antibiotics that can be further investigated to obtain propolis-based formulation with antibacterial properties. Subsequent in vitro and in vivo studies evaluating the safety and efficacy are intended to consider propolis in veterinary therapeutic protocols.


2020 ◽  
Vol 42 (1) ◽  
Author(s):  
Chu Thi Thu Ha ◽  
Tran Huy Thai ◽  
Le Ngoc Diep ◽  
Dinh Thi Thu Thuy ◽  
Nguyen Duc Ky ◽  
...  

The chemical composition of the essential oil obtained by hydrodistillation of the twigs and leaves of Magnolia macclurei collected in Hoang Su Phi, Ha Giang Province, Vietnam was analyzed using GC/MS. A total of 38 compounds were detected in essential oil, accounting for 94.49% of the oil, in which 37 compounds were identified accounting for 93.33%. cis-β-Elemene (14,30%), bicyclogermacrene (18,57%), and 10-epi-ᵧ-eudesmol (16,86%) were the main components of essential oil. The minor components present with rather high amount were: Germacrene D (2,12%), guaiol (8,52%), hinesol (2,34%), valerianol (6,76%), and bulnesol (2,93%). Antibiotic activity of the essential oil sample was tested against a Gram positive bacteria Staphylococcus aureus, a Gram negative bacteria Escherichia coli, and a yeast Candida albicans using an agar disk diffusion method. All three microorganisms tested are resistant to the oil from twigs and leaves of M. macclurei. That is exhibited through the inhibitory zone diameters ranging from 4 to 12 mm.


2021 ◽  
Vol 22 (2) ◽  
Author(s):  
YACINE MOHAMADI ◽  
TAKIA LOGRADA ◽  
Messaoud Ramdani ◽  
GILLES FIGUEREDO ◽  
PIERRE CHALARD

Abstract. Mohamadi Y, Lograda T, Ramdani M, Figueredo G, Chalard P. 2021. Chemical composition and antimicrobial activity of Myrtus communis essential oils from Algeria. Biodiversitas 22: 933-946.  The objectives of this study were to determine the chemical composition and the antibacterial activities of Myrtus communis essential oils. The aerial parts of the myrtle were collected from nineteen localities of the Algerian coast, and subjected to hydrodistillation. The chemical composition of essential oils was analyzed using gas chromatography coupled with mass spectrometry (GC/MS). The antibacterial activity was evaluated by the disk diffusion method against ten bacterial species. Essential oils of M. communis was pale yellow oils, very fragrant, with an average yield of 0.68 ± 0.6%. Forty-six compounds were identified, representing an average of 99.17 ± 0.6% of total oils. The α-pinene was the most abundant component in the essential oils of all populations studied, with a mean of 50.1 ± 10.3%, followed by 1,8-cineole (22.27 ± 4.32%), limonene (5.16 ± 2.67%), linalool (3.79 ± 2.97%), α-terpineol (2.75 ± 1.12%), geranyl acetate (1.95 ± 0.7%) and methyl eugenol (1.40 ± 0.98%). Myrtenyl acetate was only observed in four populations with significant levels (from 10.66 to 20.94%). Six chemotypes have been identified in these oils. The α-pinene – 1,8-cineole - limonene chemotype was found in the majority of populations. The chemotype of α-pinene - 1,8-cineole - α-terphenyl acetate characterizes the Oued El-Aneb population. The α-pinene - neryl formate - limonene chemotype is observed in the Honaïn population. The remaining populations are characterized by myrtenyl acetate. The essential oils of M. communis were effective against Staphylococcus aureus ATCC 25923, Salmonella enterica ATCC35659, Proteus mirabilis, and Escherichia coli ATCC 25922; however, no significant effect was observed on Klebsiella pneumonia ATCC 700603 and Serratia liquefaciens.


2018 ◽  
Vol 19 (45) ◽  
pp. 38-43 ◽  
Author(s):  
Uugangerel Erdenetsogt ◽  
Choijamts Gotov ◽  
Kerstin Voigt ◽  
Stefan Bartram ◽  
Wilhelm Boland ◽  
...  

The chemical composition and antimicrobial activity of the essential oil from the aerial parts of Pyrethrum pulchrum Ledeb. were investigated. Dried plant material was hydro-distillated yielding 0.1% of essential oil. The oil was analyzed by GC-MS techniques. Fifty-five compounds were identified representing 99.7% of the total oil composition. Camphor was the predominant compound (33.9%) followed by linalool (21.1%) and α-pinene (9.0%). The antimicrobial activity of the oil was determined using the disk diffusion method against Gram-positive bacteria (Bacillus subtilis, Staphylococcus aureus and Enterococcus faecalis), Gram-negative bacteria (Pseudomonas aeruginosa and Escherichia coli), Mycobacterium vaccae and fungi (Candida albicans, Sporidiobolus salmonicolor and Penicillum notatum). The essential oil of P. pulchrum displays an intermediate activity against selected bacteria.


2019 ◽  
Vol 16 (2) ◽  
pp. 403-409
Author(s):  
Deepika Pathak ◽  
Kruti M. Dave ◽  
Luluaa Aliasgar

Plants have been played an important role as a resource of natural medicines for human health, from the long period of time. Some plants show antimicrobial properties. The most important merit of using plant derived medicines is that they are low priced, readily available and showing minor side effect. The present study deals with the antibacterial potential of crude extracts of petals of Rosa indica. Through agar disk diffusion method the antimicrobial potential of Rosa indica was examined. Petals were collected, dried and its crude extract was obtained. Methanol and acetone were used as the extraction solvent. These extract were examined against two gram-positive (Staphylococcus aureus, Bacillus cereus) and two gram-negative (E-coli, Salmonella typhi) bacteria. Extracts prepared by all solvents showed antimicrobial action and established zone(s) of inhibition. By performing phytochemical tests we have observed positive results for flavanoid, tannin and alkaloid. These phytochemical have capability to inhibit the growth of microorganism or can fight against microorganism. Rich source of compounds have been obtained in this particular plant. For inhibition of multiple drug resistant microorganisms, this extract can be tested. For making herbal drugs, extract of Rosa indica may prove to be useful as an advance step in future.


2017 ◽  
Vol 12 (2) ◽  
pp. 1934578X1701200
Author(s):  
Gordana S. Stojanović ◽  
Veneta Kapchina-Toteva ◽  
Mariq Angelova Rogova ◽  
Snežana Č. Jovanović ◽  
Zhenya Yordanova ◽  
...  

The present study reports the chemical composition of headspace volatiles (HS) and acetone extracts of the endemic Bulgarian species Achillea thracica Velen. from its natural habitat (N), in vitro propagated (IN) and ex vitro established (EX) plants. Additionally, acetone extracts were tested by a disk diffusion method for antibacterial activity. Irregular monoterpenes were the most abundant HS volatile constituents, while O,O-dimethyl quercetin was the most abundant flavonoid in the acetone extracts. The secondary metabolites of A. thracica grown in its natural habitat (N), propagated in vitro (IN) and ex vitro established (EX) showed that the qualitative composition is mutually similar, but there are differences in the quantitative composition. Considering antibacterial activity, IN and EX samples showed moderate activity against Pseudomonas aeruginosa and Escherichia coli.


2020 ◽  
Vol 12 (2) ◽  
Author(s):  
Vesna Gojković Cvjetković ◽  
Željka Marjanović-Balabań ◽  
Marijana Kapović-Solomuń ◽  
Ljiljaná́ Stanojevic ◽  
Jelená́ Stanojevic ◽  
...  

The quality of the essential oil obtained from white pine wood greenery from the territory of the Republic of Srpska was analyzed by examining the chemical composi- tion and antimicrobial activity of samples produced by the hydro-distillation process in industrial production conditions. The chemical composition of the essential oil was analyzed by GC/MS and GC/FID analysis. The antimicrobial activity of the ob- tained white pine oil was investigated for its possible use as an antimicrobial agent. The obtained results prove the presence of 37 components in the analyzed sam- ple, which represents 98.5% of the total components present. The most common are monoterpene hydrocarbons, followed by sesquiterpene hydrocarbons, much less represented are monoterpenes containing oxygen and aromatic compounds, while aliphatic esters are found only in traces. Antimicrobial activity was examined using the disk diffusion method. The results showed large zones of inhibition, both for the tested oil sample and for all eight analyzed bacterial strains (from 12.00 ± 0.00 to 17.33 ± 1.15mm), as well as for the fungal strain. The chemical composition and antimicrobial action of essential white pine oiled from plant material originating from the territory of the Republic of Srpska indicates its significant phytomedical potential.


2020 ◽  
Vol 42 (3) ◽  
Author(s):  
Chu Thi Thu Ha ◽  
Bui Van Thanh ◽  
Dinh Thi Thu Thuy

Leaf essential oil of Magnolia coriacea (Hung T. Chang & B. L. Chen) Figlar growing wild in the Bat Dai Son Nature Reserve, Ha Giang Province, Viet Nam was obtained by hydrodistillation and its chemical composition was analyzed using GC/MS. In total, 45 compounds were detected in the essential oil, accounting for 87.1% of the oil, in which 37 compounds were identified accounting for 66.9%. Bicyclogermacrene (12.6%) and spathulenol (17.0%) were the main components of the leaf essential oil of M. coriacea. Antimicrobial activity of the essential oil sample was tested against three microorganism strains using an agar disk diffusion method. The results show that the inhibitory zone diameters ranged from 8.5 to 20.5 mm. Median inhibitory concentration (IC50) and minimum inhibitory concentration (MIC) of the essential oil was determined using microdilution broth susceptibility assay against seven test microorganism strains. Bacillus subtilis had the highest sensitivity with IC50 and MIC values of 185.9 and 512 µg/mL, respectively.  


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