scholarly journals Detection of Salivary miRNAs That Predict Chronic Periodontitis Progression: A Cohort Study

2021 ◽  
Vol 18 (15) ◽  
pp. 8010
Author(s):  
Kohei Fujimori ◽  
Toshiki Yoneda ◽  
Takaaki Tomofuji ◽  
Daisuke Ekuni ◽  
Tetsuji Azuma ◽  
...  
Keyword(s):  
Cohort Study ◽  
Chronic Periodontitis ◽  
Characteristic Curve ◽  
Control Group ◽  
Discrimination Power ◽  
Whole Saliva ◽  
Supportive Periodontal Therapy ◽  
New Biomarkers ◽  
Unstimulated Whole Saliva ◽  
Attachment Level

The aim of this two-year cohort study was to investigate salivary microRNAs (miRNAs) that predict periodontitis progression. A total of 120 patients who underwent supportive periodontal therapy were recruited. Unstimulated whole saliva was collected at baseline. Two years later, 44 patients were followed up (median age, 67.1 years) and divided into two groups: progression group (n = 22), with one or more sites with clinical attachment level (CAL) progression (>3 mm compared with baseline) or tooth extraction due to periodontitis progression; and the control group (n = 22), which did not exhibit CAL progression. In the microarray analysis of salivary miRNAs, hsa-miR-5571-5p, hsa-miR-17-3p, hsa-let-7f-5p, hsa-miR-4724-3p, hsa-miR-99a-5p, hsa-miR-200a-3p, hsa-miR-28-5p, hsa-miR-320d, and hsa-miR-31-5p showed fold change values <0.5 or ≥2.0 in the progression group compared with the control group (p < 0.05). On receiver operating characteristic curve analysis, areas under the curves of hsa-miR-5571-5p, hsa-let-7f-5p, hsa-miR-99a-5p, hsa-miR-28-5p, and hsa-miR-320d were >0.7, indicating fair discrimination power. The expressions of salivary hsa-miR-5571-5p, hsa-let-7f-5p, hsa-miR-99a-5p, hsa-miR-28-5p, and hsa-miR-320d were associated with periodontitis progression in patients with chronic periodontitis. These salivary miRNAs may be new biomarkers for progression of periodontitis, and monitoring them may contribute to new diagnostics and precision medicine for periodontitis.

Optimization of the terms of supportive periodontal therapy using photoactivated disinfection

2019 ◽  
Vol 24 (2) ◽  
pp. 121-126 ◽  
Author(s):  
V. G. Atrushkevich ◽  
L. Yu. Orekhova ◽  
O. O. Yanushevich ◽  
E. Yu. Sokolova ◽  
E. S. Loboda
Keyword(s):  
Chronic Periodontitis ◽  
Adjunctive Treatment ◽  
Control Group ◽  
Clinical Parameters ◽  
Ozone Therapy ◽  
Pocket Depth ◽  
Clinical Attachment Loss ◽  
Supportive Periodontal Therapy ◽  
Tannerella Forsythensis ◽  
Observation Period

Relevance: to indentify if periodontal treatment which is presented by photoactivated disinfection (PAD) adjunctively to scaling and root planing (SRP) yield better outcomes than ozone therapy as an adjunct to SRP in periodontitis.Materials and methods: we examined 57 (mean age 49,3 ± 1,02) patients with chronic periodontitis, divided into groups, SRP + PAD, SRP + ozone therapy and SRP alone. Subgingival plaque samples were subjected to DNA extraction and real time PCR amplifcation for detection Porphyromonas gingivalis (Pg), Prevotella intermedia (Pi), Tannerella forsythensis (Tf), Treponema denticola (Td), Aggregatibacter actinomycetemcomitans (Aa). The amount of periodontopathogens and clinical parameters including plaque index, clinical attachment loss, pocket depth, bleeding on probing were measured at baseline, after 40, 90 and 180 days.Results: the results in groups of PAD+SRP and ozone therapy+SRP showed an improvement in all clinical parameters PI, BOP, PD, CAL and the quantity of Pg, Td and Tf compared to the control group during an observation period.Conclusion: the results showed additional benefts from PAD and ozone therapy as an adjunctive treatment to SRP for patients of chronic periodontitis.

scholarly journals Clinical and Microbiological Evaluation of Local Doxycycline and Antimicrobial Photodynamic Therapy during Supportive Periodontal Therapy: A Randomized Clinical Trial

Antibiotics ◽  
2021 ◽  
Vol 10 (3) ◽  
pp. 277
Author(s):  
Raluca Cosgarea ◽  
Sigrun Eick ◽  
Ionela Batori-Andronescu ◽  
Søren Jepsen ◽  
Nicole B. Arweiler ◽  
...  
Keyword(s):  
Photodynamic Therapy ◽  
Bacterial Species ◽  
Periodontal Therapy ◽  
Local Drug Delivery ◽  
Control Group ◽  
Periodontal Pathogens ◽  
Supportive Periodontal Therapy ◽  
Group A ◽  
Group B ◽  
Attachment Level

The aim of this study was to evaluate the clinical and microbiological effects of subgingival instrumentation (SI) alone or combined with either local drug delivery (LDD) or photodynamic therapy (PDT) in persistent/recurrent pockets in patients enrolled in supportive periodontal therapy (SPT). A total of 105 patients enrolled in SPT were randomly treated as follows: group A (n = 35): SI +PDT and 7 days later 2nd PDT; group B (n = 35): SI+LDD; group C (n = 35): SI (control). Prior intervention, at 3 and 6 months after therapy, probing pocket depths, clinical attachment level, number of treated sites with bleeding on probing (n BOP), full mouth plaque and bleeding scores (gingival bleeding index, %BOP) were recorded. At the same time points, 8 periodontopathogens were quantitatively determined. All three treatments resulted in statistically significant improvements (p < 0.05) of all clinical parameters without statistically significant intergroup differences (p > 0.05). Several bacterial species were reduced in both test groups, with statistically significantly higher reductions for LDD compared to PDT and the control group. In conclusion, the present data indicate that: (a) In periodontal patients enrolled in SPT, treatment of persistent/recurrent pockets with SI alone or combined with either PDT or LDD may lead to comparable clinical improvements and (b) the adjunctive use of LDD appears to provide better microbiological improvements for some periodontal pathogens than SI alone or combined with PDT.

Correlation of salivary neopterin and plasma fibrinogen levels in patients with chronic periodontitis and/or type 2 diabetes mellitus

Pteridines ◽  
2017 ◽  
Vol 28 (3-4) ◽  
pp. 177-183 ◽  
Author(s):  
Angel Fenol ◽  
V.R. Swetha ◽  
Sajitha Krishnan ◽  
Jayachandran Perayil ◽  
Rajesh Vyloppillil ◽  
...  
Keyword(s):  
Diabetes Mellitus ◽  
Chronic Periodontitis ◽  
Study Groups ◽  
Control Group ◽  
Diabetic Patients ◽  
Potential Biomarker ◽  
Whole Saliva ◽  
Nonsurgical Periodontal Therapy ◽  
Significant Difference

AbstractNeopterin is a novel predictor for coronary events especially in diabetic patients and also an indicator for the effectiveness of the periodontal treatment. In this study, we assessed whether salivary neopterin can be used as a potential biomarker in evaluating the risk of cardiovascular disease in type 2 diabetic patients with chronic periodontitis. Forty subjects between 25 and 75 years of age and who matched the criteria were selected and divided into four groups. Their periodontal status was evaluated. Stimulated whole saliva and blood were collected for analysis of salivary neopterin and fibrinogen and HbA1c levels, respectively. Nonsurgical periodontal therapy was carried out. Patients were recalled after 3 months, and the same procedure was repeated. A reduction in all the parameters was seen after treatment in all the four groups. Salivary neopterin levels showed significant difference (p<0.001) in the values between the study groups and the control group before treatment. After 3 months of treatment, salivary neopterin levels showed a statistical significant reduction (p<0.001) in all the study groups. Neopterin could serve as an effective tool to assess the inflammatory process related to periodontitis and diabetes mellitus and also predict future cardiovascular events in diabetic patients.

scholarly journals Laser Assisted Non-surgical Periodontal Therapy: A Double Blind, Randomized Clinical Trial

2017 ◽  
Vol 11 (1) ◽  
pp. 79-90 ◽  
Author(s):  
Joseph D. Everett ◽  
Jeffrey A. Rossmann ◽  
David G. Kerns ◽  
Ibtisam Al-Hashimi
Keyword(s):  
Laser Therapy ◽  
Chronic Periodontitis ◽  
Test Group ◽  
Control Group ◽  
Scaling And Root Planing ◽  
Clinical Attachment Level ◽  
Laboratory Parameters ◽  
Root Planing ◽  
Significant Difference ◽  
Attachment Level

The objective of this study was to examine potential benefits of using laser therapy for secular decontamination in conjunction with scaling and root planing in the treatment of chronic periodontitis. The study was performed on 173 teeth in 14 patients in a split-mouth design, one side received scaling and root planing followed by laser therapy using a carbon dioxide (CO2) laser with an ablative handpiece (test group); the contralateral side received scaling and root planing without laser (control group). Clinical and laboratory parameters were evaluated prior to treatment and at 3 and 6 months following therapy; clinical measurements were performed by two blinded examiners. The clinical parameters included measurement of gingival recession (REC), bleeding on probing (BOP), clinical attachment level (CAL), pocket depth (PD), furcation involvement (FUR), and tooth mobility (MOB). Laboratory testing to determine the levels of periodontal pathogens was performed using PCR techniques. The results of the study revealed statistically significant differences in clinical and laboratory parameters at 3 and 6 months after therapy for both test and control groups, but no significant difference was observed between the two groups. However, sites receiving laser therapy tended to show a greater decrease in probing depths, gain in clinical attachment level, and reduced bacterial levels. In conclusion, the overall results of the study suggest a potential benefit of using laser therapy in conjunction with scaling and root planing for the treatment of chronic periodontitis.

scholarly journals Detection of Salivary miRNAs Reflecting Chronic Periodontitis: A Pilot Study

Molecules ◽  
2019 ◽  
Vol 24 (6) ◽  
pp. 1034 ◽  
Author(s):  
Kohei Fujimori ◽  
Toshiki Yoneda ◽  
Takaaki Tomofuji ◽  
Daisuke Ekuni ◽  
Tetsuji Azuma ◽  
...  
Keyword(s):  
Pilot Study ◽  
Chronic Periodontitis ◽  
Pocket Depth ◽  
Cross Sectional ◽  
Relative Expression ◽  
Reverse Transcription Pcr ◽  
Probing Pocket Depth ◽  
Severe Periodontitis ◽  
Whole Saliva ◽  
Unstimulated Whole Saliva

The purpose of this cross-sectional pilot study was to find salivary microRNAs (miRNAs) reflecting periodontal condition in chronic periodontitis. One hundred and twenty chronic periodontitis patients (mean age, 68.4 years) participated in the study, from whom unstimulated whole saliva was collected. A multiphase study was conducted to explore salivary miRNAs as biomarkers of periodontitis. At first, a polymerase chain reaction (PCR) array was performed to compare salivary miRNAs profiles in no and mild (no/mild) and severe periodontitis patients. Next, the relative expression of salivary miRNAs on individual samples was assessed by real-time reverse transcription-PCR. The numbers (%) of patients were 26 (21.6%, no/mild), 58 (48.3%, moderate) and 36 (30.0%, severe), respectively. Among 84 miRNAs, only the relative expression of hsa-miR-381-3p in the severe periodontitis group was significantly higher than that of the no/mild periodontitis group (p < 0.05). Among the 120 patients, there was also a significant correlation between the relative expression of hsa-miR-381-3p and the mean probing pocket depth (PPD) (r = 0.181, p < 0.05). Salivary hsa-miR-381-3p was correlated with periodontitis condition in chronic periodontitis patients.

scholarly journals EVALUATION OF SALIVARY HEPATOCYTE GROWTH FACTOR LEVELS IN CHRONIC PERIODONTITIS PATIENTS BEFORE AND AFTER NON-SURGICAL PERIODONTAL TREATMENT

2020 ◽  
pp. 8-10
Author(s):  
Madhavi T ◽  
Mathew John ◽  
Bindu Rachel Thomas ◽  
Sabari Chandramohan ◽  
Aswathy S ◽  
...  
Keyword(s):  
Growth Factor ◽  
Hepatocyte Growth Factor ◽  
Chronic Periodontitis ◽  
Periodontal Treatment ◽  
Periodontal Tissues ◽  
Whole Saliva ◽  
Before And After ◽  
Unstimulated Whole Saliva ◽  
Relationship Of ◽  
Hepatocyte Growth

Hepatocyte Growth Factor (HGF) or Scatter Factor (SF) is a growth factor which restrains the reconstruction of profound periodontal tissues. The study estimated the salivary HGF levels in unstimulated whole saliva in chronic periodontitis patients prior to treatment and also at 2nd and 6th week after non-surgical periodontal treatment and also aimed to determine the relationship of salivary HGF levels with the clinical periodontal parameters. This was a prospective-analytical study which included 45 subjects with chronic periodontitis. The highest mean concentration of HGF was obtained at the baseline and the lowest mean HGF concentration was obtained at the 6th week. There was significant decrease in the salivary HGF levels among the patients subsequent to scaling and root planing. Moreover the salivary HGF levels indicated a positive correlation with the clinical parameters at the sixth week. The above results propose that salivary HGF levels could be utilized as a biomarker for checking the response to periodontal treatment in chronic periodontitis patients.

scholarly journals YES! OXIDATIVE STRESS MAY BE A RISK FACTOR FOR CHRONIC PERIODONTITIS PATIENTS

2020 ◽  
pp. 1-3
Author(s):  
Archith Mannan ◽  
Mathew John ◽  
Bindu Rachel Thomas ◽  
Sabari Chandramohan ◽  
Aswathy S. ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Chronic Periodontitis ◽  
Defense Mechanisms ◽  
Alveolar Bone ◽  
Inflammatory Disorder ◽  
Complex Interactions ◽  
Probing Depth ◽  
Whole Saliva ◽  
Potential Damage ◽  
Attachment Level

Oxidative stress is defined as the condition occurring when the physiological balance between oxidants and antioxidants is disrupted in favor of the former with potential damage for the organism. The pathological events leading to the destruction of the periodontium encompasses complex interactions involving an imbalance between oxidant and antioxidant enzymatic and non-enzymatic defense mechanisms. Periodontitis is an oral inflammatory disorder affecting alveolar bone, gingiva, and periodontal ligament initiated by the complex interaction between the presence of microorganisms (periodontopathic bacteria) and the host’s immune response. Whole saliva samples were collected from 25 chronic periodontitis patients. Saliva samples were centrifuged and the supernatant was used to determine the 8-OHdG levels with a competitive ELISA kit. To determine the clinical condition of each subject, the Bleeding on Probing (BOP), Clinical attachment level (CAL), and Pocket probing depth (PPD) were measured. This study shows significant correlation between increased values of oxidative stress biomarker and clinical parameters in chronic periodontitis patients.

scholarly journals p53 as a Neoplastic Biomarker in Patients with Erosive and Plaque Like Forms of Oral Lichen Planus

2013 ◽  
Vol 14 (1) ◽  
pp. 1-3 ◽  
Author(s):  
Iraj Mirzaii-Dizgah ◽  
Farzaneh Agha-Hosseini
Keyword(s):  
Clinical Significance ◽  
Lichen Planus ◽  
Oral Lichen Planus ◽  
Control Group ◽  
Whole Saliva ◽  
The Mean ◽  
Unstimulated Whole Saliva ◽  
Oral Lichen ◽  
Mean Concentration

ABSTRACT Aim Unstimulated whole salivary p53 was assessed in patients suffering from erosive and plaque-like form of oral lichen planus (OLP). Materials and methods Eighteen patients with erosive form, 17 patients suffering from plaque-like form and 38 noninvolvement subjects were enrolled. The unstimulated whole saliva p53 level was assayed by ELISA. Results The mean concentration of salivary p53 was significantly higher in patients with plaque-like form compared to both patients with erosive form and the control group. Conclusion We conclude that plaque like form of OLP is important in view of the potential for malignancy and is not safety form. Clinical significance It seems that all forms of OLP must be considered accurately, should be followed up with biannual examinations, and if possible, assessment of salivary p53 every year. How to cite this article Agha-Hosseini F, Mirzaii-Dizgah I. p53 as a Neoplastic Biomarker in Patients with Erosive and Plaque Like Forms of Oral Lichen Planus. J Contemp Dent Pract 2013;14(1):1-3.

scholarly journals The Use of Controlled Release Locally Delivered 10% Doxycycline Hyclate Gel as an adjunct to Scaling and Root Planing in the Treatment of Chronic Periodontitis: Clinical and Microbiological Results

2013 ◽  
Vol 14 (6) ◽  
pp. 1080-1086 ◽  
Author(s):  
Md Jalaluddin ◽  
Shabeer Ahamed ◽  
Imran Khalid ◽  
Ninad Moon ◽  
TK Shafi ◽  
...  
Keyword(s):  
Controlled Release ◽  
Chronic Periodontitis ◽  
Control Group ◽  
Clinical Parameters ◽  
Scaling And Root Planing ◽  
Doxycycline Hyclate ◽  
Pocket Depth ◽  
Probing Pocket Depth ◽  
Root Planing ◽  
Attachment Level

ABSTRACT Background Local delivery of antimicrobial agents provides higher concentration of the drug in the periodontal site for longer periods than systemically delivered methods. In the present study an attempt is made to know the efficacy of controlled local drug delivery of doxycycline as an adjunctive treatment in the management of chronic periodontitis. Materials and methods A total of 12 patients, diagnosed as Chronic Periodontitis in the age of 25 to 55 years, were selected of both the sexes in this study. They were divided into Experimental group consisted of 30 sites who received complete scaling and root planing (SRP) followed by placement of Atridox gel and control group consisted of 30 sites who received only SRP. Clinical parameters were recorded at, baseline, days 30, 90 and 180. Parameters were plaque index, gingival index, gingival bleeding index and microbial analysis, probing pocket depth and Clinical attachment level (CAL). The microbiological analysis was done at baseline visit and at 90th day. Probing pocket depth and CAL were recorded only on day 0 and 180th day. Results In 180 days study, both the groups exhibited a significant improvement in periodontal status. Significant gain in attachment level was observed in both the group. Between both the groups the clinical parameters in the experimental groups exhibited better results as compared to the control group. Both the groups exhibited significant reduction in the number of spirochetes. Conclusion Combination therapy of SRP and 10% DH gel demonstrated better results at all levels suggesting that this therapy can play a significant role as an adjunct to SRP in the management of chronic periodontitis. How to cite this article Ahamed S, Jalaluddin Md, Khalid I, Moon N, Shafi TK, Ali FM. The Use of Controlled Release Locally Delivered 10% Doxycycline Hyclate Gel as an adjunct to Scaling and Root Planing in the Treatment of Chronic Periodontitis: Clinical and Microbiological Results. J Contemp Dent Pract 2013;14(6):1080-1086.

scholarly journals Clinical Assessment of Drug Adjunctive Therapy Effects in Association with Chronic Generalized Periodontitis and Osteoporotic Disease

2015 ◽  
Vol 19 (3) ◽  
pp. 158-162
Author(s):  
Irina-Georgeta Ursarescu ◽  
Liliana Pasarin ◽  
Sorina Solomon ◽  
Alexandra Martu ◽  
Silvia Martu
Keyword(s):  
Chronic Periodontitis ◽  
Adjunctive Therapy ◽  
Control Group ◽  
Study Group ◽  
Clinical Effects ◽  
Clinical Attachment Level ◽  
Probing Depth ◽  
Periodontal Debridement ◽  
Significant Clinical Improvement ◽  
Attachment Level

SummaryAim: The present study proposes an assessment of the clinical effects on periodontal level generated by the adjunctive periodontal therapy with sub-antimicrobial doses of doxycycline in patients with chronic periodontitis and osteoporosis.Materials and Methods: The study group comprised 26 patients with chronic periodontitis and osteoporosis, divided in the study group (classical periodontal debridement and sub-antimicrobial doses of doxycycline for 3 months) and the control group (classical debridement only). We analyzed the periodontal parameters (probing depth, clinical attachment level, PBI and PI indices) at baseline, in the last day of medication and at 3 months after the drug therapy.Results and Discussion: The reduction of the moderate and profound pockets was higher for the group with drug adjunctive therapy. The sites with an initial depth of 0-3mm in the study group presented also a slight attachment gain.Conclusions: The therapy with sub-antimicrobial doses of doxycycline generated significant clinical improvement in patients with chronic periodontitis and osteoporosis, an improvement which can reduce the necessity of surgical procedures.

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