scholarly journals p53 as a Neoplastic Biomarker in Patients with Erosive and Plaque Like Forms of Oral Lichen Planus

2013 ◽  
Vol 14 (1) ◽  
pp. 1-3 ◽  
Author(s):  
Iraj Mirzaii-Dizgah ◽  
Farzaneh Agha-Hosseini
Keyword(s):  
Clinical Significance ◽  
Lichen Planus ◽  
Oral Lichen Planus ◽  
Control Group ◽  
Whole Saliva ◽  
The Mean ◽  
Unstimulated Whole Saliva ◽  
Oral Lichen ◽  
Mean Concentration

ABSTRACT Aim Unstimulated whole salivary p53 was assessed in patients suffering from erosive and plaque-like form of oral lichen planus (OLP). Materials and methods Eighteen patients with erosive form, 17 patients suffering from plaque-like form and 38 noninvolvement subjects were enrolled. The unstimulated whole saliva p53 level was assayed by ELISA. Results The mean concentration of salivary p53 was significantly higher in patients with plaque-like form compared to both patients with erosive form and the control group. Conclusion We conclude that plaque like form of OLP is important in view of the potential for malignancy and is not safety form. Clinical significance It seems that all forms of OLP must be considered accurately, should be followed up with biannual examinations, and if possible, assessment of salivary p53 every year. How to cite this article Agha-Hosseini F, Mirzaii-Dizgah I. p53 as a Neoplastic Biomarker in Patients with Erosive and Plaque Like Forms of Oral Lichen Planus. J Contemp Dent Pract 2013;14(1):1-3.

Association of Oral Lichen Planus with Dyslipidemia in Patients Of Rahim Yar Khan

2021 ◽  
Vol 15 (11) ◽  
pp. 3136-3137
Author(s):  
Nazia Hanif ◽  
Tahir Hassan ◽  
Muhammad Khurram Shahzad ◽  
Raheel Tahir ◽  
Madiha Anwar ◽  
...  
Keyword(s):  
Total Cholesterol ◽  
Lichen Planus ◽  
Oral Lichen Planus ◽  
Ldl Cholesterol ◽  
Hdl Cholesterol ◽  
Serum Total Cholesterol ◽  
Control Group ◽  
Chronic Inflammatory Condition ◽  
The Mean ◽  
Oral Lichen

Introduction: Oral lichen planus (LP) is a chronic inflammatory condition and this inflammation leads to alteration in lipid metabolism and increase the cardiovascular risk factors. Objective: To determine the prevalance of dyslipidemias in patient with oral lichen planus coming in dermatology outdoor of Sheikh Zayed Hoapital Rahim Yar Khan. Methods: It was a case control study that was conducted at Department of Dermatology, Sheikh Zayed Hospital Rahim Yar Khan during October 2016 to April 2017. In this study there were total 50 cases of oral LP and 50 controls of both genders with age range of 15 to 60 years. The cases of both the groups underwent fasting lipid profile in terms to look for serum total cholesterol, triglyceride, LDL cholesterol and HDL cholesterol. Results: In this study there were total 100 cases out of which 50 were cases of oral LP and 50 were controls. In cases groups there were 27 (54%) males and 23 (46%) females while in control group there were same number of males and females were taken with p= 0.85. The mean age in case group was 37.22±8.15 years while in control group was 35.66± 7.78 years while the mean BMI was 24.42±2.21 and 25.11±3.01 respectively. The mean difference in cases and controls in terms of serum total cholesterol, TG, HDL Cholesterol and LDL cholesterol was found statistically significant. Conclusion: Dyslipidemias are common in cases with oral lichen planus and all its parameters have significant association with it especially TG. Keywords; LP, Dyslipidemia, LDL, HDL, TG

scholarly journals The Assessment of Angiogenesis by Microvessel Density in Patients With Atrophic/Erosive or Reticular Oral Lichen Planus

2021 ◽  
Author(s):  
Maryam Mardani ◽  
Azadeh Andisheh-Tadbir ◽  
Mahya Haghparast
Keyword(s):  
Lichen Planus ◽  
Microvessel Density ◽  
Oral Lichen Planus ◽  
Precancerous Lesions ◽  
Oral Disease ◽  
Staining Procedure ◽  
Control Group ◽  
Significant Difference ◽  
The Mean ◽  
Oral Lichen

The role of angiogenesis in the development of chronic inflammatory diseases, including oral lichen planus (OLP), is of great concern. The most representative method for the assessment of angiogenesis is the semi-quantification of microvessel density (MVD) using specific markers such as CD105. We aimed to assess the MVD in patients with OLP and find its clinical significance to differentiate the atrophic/erosive forms from reticular ones. This cross-sectional study was conducted on 82 patients with clinically and histopathologically proven cases of OLP, including reticular (n=52) or atrophic/erosive (n=30) lesions. The control group comprised 82 age- and sex-matched subjects without any oral disease. To assess the MVD using CD105, tissue blocks were sliced, and the immunoexpression of CD105 was measured by the standard immunohistochemical staining procedure. The mean value of MVD in OLP patients was significantly higher than that in the controls (14.61±12.48 vs. 8.67±1.76, P<0.0001). Furthermore, there was a significant difference in the mean MVD value between reticular and atrophic/erosive lesions (8.19±7.13 vs. 25.73±12.06, P=0.001). However, no significant difference was observed between the reticular lesions and normal tissues (P=0.58). An increased level of CD105 in OLP patients can improve our knowledge about the causes and mechanisms of the disease. The CD105-MVD assessment might be a useful method for semiquantitative measurement of angiogenesis in OLP patients as well as differentiating its clinical forms; therefore, it can open new vistas for formulating strategies based on antiangiogenic treatments for the management of OLP and other precancerous lesions.

scholarly journals Matrix Metalloproteinases Neutralizing Agents: A Possible Treatment Modality for Oral Lichen Planus (Preliminary Study)

2021 ◽  
Author(s):  
Hossam Elsabagh ◽  
Yasmine Gaweesh ◽  
Jaylane Ghonima
Keyword(s):  
Matrix Metalloproteinases ◽  
Disease Severity ◽  
Lichen Planus ◽  
Oral Lichen Planus ◽  
Test Group ◽  
Control Group ◽  
Oral Diseases ◽  
The Mean ◽  
Oral Lichen

Abstract Background: Oral lichen planus is one of the most prevalent oral diseases. Matrix metalloproteinases have been implicated in its pathogenesis thus this study was executed to evaluate the effectiveness of matrix metalloproteinases neutralizing agents in the treatment of oral lichen planus. Methods: Patients were assigned to either control group who received topical corticosteroids and antifungal treatment, or test group who received matrix metalloproteinases neutralizing spray. All patients were evaluated for disease severity and pain at baseline, 1 week, and 4 weeks after treatment. Results: The mean pain score in test and control groups was 9.00 (0.89), and 9.33 (0.82), respectively, at baseline while it was 2.33 (1.75) and 2.83 (1.72) at 4th week follow up. The mean disease severity score was 4.33 (0.52) for test group and 4.50 (0.55) for control group at baseline and decreased to 1.83 (0.75) and 2.17 (0.98) at 4th week follow up. Despite the better results seen in test group on the expense of control group, no statistically significant differences could be detected between groups at any timepoint. Conclusion: The use of matrix metalloproteinases neutralizing agents might be an equally effective sole treatment for erosive oral lichen planus compared to the conventional treatment, without the risk of secondary candidiasis. Trial registration: The study was registered retrospectively at ClinicalTrials.gov (NCT04336488), date (07/04/2020)

scholarly journals Quantitative Analysis of Salivary TNF-αin Oral Lichen Planus Patients

2015 ◽  
Vol 2015 ◽  
pp. 1-5 ◽  
Author(s):  
T. Malarkodi ◽  
S. Sathasivasubramanian
Keyword(s):  
Lichen Planus ◽  
Oral Lichen Planus ◽  
Necrosis Factor Alpha ◽  
Mean Values ◽  
Binomial Proportion ◽  
Whole Saliva ◽  
Factor Alpha ◽  
The Mean ◽  
Group B ◽  
Oral Lichen

Objective. The aim of this study was to quantitatively evaluate the salivary tumor necrosis factor-alpha (TNF-α) level in oral lichen planus patients and to compare the levels of TNF-αbetween saliva and serum of OLP and controls.Methods. Serum and whole saliva from 30 patients with active lesions of oral lichen planus (OLP) and 30 healthy persons were investigated for the presence of TNF-αby enzyme immunoassay. Student’s independentt-test and two-sample binomial proportion test were used to calculate significance of the mean values of TNF-alpha in serum and saliva and to determine the proportions of the detected and nondetected samples in both groups.Results. Proportion of detection and the mean of detectability between saliva and serum of Group B show an almost equal value, which suggests that saliva can be a good alternate to serum to analyze TNF-αin oral lichen planus patients.

Salivary mir-27b Expression in Oral Lichen Planus Patients: A Series of Cases and a Narrative Review of Literature

2020 ◽  
Vol 19 (31) ◽  
pp. 2816-2823 ◽  
Author(s):  
Dario Di Stasio ◽  
Laura Mosca ◽  
Alberta Lucchese ◽  
Donatella Delle Cave ◽  
Hiromichi Kawasaki ◽  
...  
Keyword(s):  
Lichen Planus ◽  
Oral Lichen Planus ◽  
Inflammatory Diseases ◽  
Biological Fluids ◽  
Critical Role ◽  
Invasive Procedure ◽  
Control Group ◽  
Study Group ◽  
Immune Functions ◽  
Oral Lichen

Background: microRNAs play a critical role in auto-immunity, cell proliferation, differentiation and cell death. miRNAs are present in all biological fluids, and their expression is essential in maintaining regular immune functions and preventing autoimmunity, whereas miRNA dysregulation may be associated with the pathogenesis of autoimmune and inflammatory diseases. Oral lichen planus (OLP) is an inflammatory disease mediated by cytotoxic T cells attack against epithelial cells. The present study aims to perform a specific microRNA expression profile through the analysis of saliva in this disease. Methods: The study group was formed by five patients (mean age 62.8±1.98 years; 3 females/2 males) affected by oral lichen planus and control group by five healthy subjects (mean age 59.8 years±2.3; 3 females/ 2 males); using a low-density microarray analysis, we recorded a total of 98 differentially expressed miRNAs in the saliva of patients with oral lichen planus compared to the control group. The validation was performed for miR-27b with qRT-PCR in all saliva samples of oral lichen planus group. Results: 89 miRNAs were up-regulated and nine down-regulated. In details, levels of miR-21, miR- 125b, miR-203 and miR15b were increased (p<0.001) in study group while levels of miR-27b were about 3.0-fold decreased compared to controls (p<0.001) of miR-27b expression in OLP saliva. QRTPCR validation confirmed the down regulation of miR-27b in all saliva samples. Conclusions: Collecting saliva samples is a non-invasive procedure and is well accepted by all patients. microRNAs can be readily isolated and identified and can represent useful biomarkers of OLP.

scholarly journals Evaluation of Cellular Proliferative Activity in Patients with Oral Lichen Planus and Hepatitis C through AgNOR Method

2014 ◽  
Vol 25 (6) ◽  
pp. 461-465
Author(s):  
João Paulo De Carli ◽  
Soluete Oliveira da Silva ◽  
Maria Salete Sandini Linden ◽  
Carmen Silvia Busin ◽  
Luiz Renato Paranhos ◽  
...  
Keyword(s):  
Hepatitis C ◽  
Lichen Planus ◽  
Oral Mucosa ◽  
Oral Lichen Planus ◽  
Clinical Signs ◽  
Hcv Infection ◽  
Control Group ◽  
Proliferative Potential ◽  
Group 2 ◽  
Oral Lichen

The objective of this study was to evaluate the cellular proliferative potential of oral lichen planus (OLP) lesions from patients without hepatitis C virus (HCV) by means of AgNOR method, as well as the cellular proliferative potential of the normal oral mucosa from patients with HCV, treated or untreated by interferon and ribavirin. A cross-sectional study was developed to investigate four groups: 10 HCV+ patients without clinical signs of OLP who had never been treated for HCV infection - Group 1; 10 HCV+ patients that were under interferon and ribavirin treatment - Group 2; 15 patients with reticular OLP lesions histopathologically confirmed, without HCV - Group 3; and 15 blood donors without HCV infection and no clinical signs of OLP GROUP 4 Control Group. The cytological material of all groups was collected by the liquid-based cytology technique. Then, the sedimented material from each patient was filled with the Nucleolar Organizer Regions impregnation by silver method (AgNOR). The count of NORs was performed on 100 epithelial cell nuclei per patient using the Image Tool(tm) software. The Tukey HSD test was used to compare the median value of NORs among the groups and showed that the oral mucosa of HCV+ patients previously treated with anti-HCV drugs (GROUP 2), presented a higher average number of NORs in relation to others (p<0.05). The anti-HCV treatment may be related to increased cell proliferation of oral mucosa, indicating a possible relationship between OLP and HCV+ patients treated with interferon and ribavirin.

scholarly journals Is Topical Application of Hyaluronic Acid in Oral Lichen Planus Effective? A Randomized Controlled Crossover Study

2020 ◽  
Vol 10 (22) ◽  
pp. 7988
Author(s):  
Corinna Bruckmann ◽  
Rudolf Seemann ◽  
Klemens Rappersberger ◽  
Xiaohui Rausch-Fan ◽  
Hady Haririan ◽  
...  
Keyword(s):  
Hyaluronic Acid ◽  
Lichen Planus ◽  
Oral Lichen Planus ◽  
Clinical Severity ◽  
Clinical Parameters ◽  
Whole Saliva ◽  
Before And After ◽  
Crossover Phase ◽  
Lost To Follow Up ◽  
Oral Lichen

Hyaluronic acid (HA) has anti-inflammatory and anti-edematous effects and, thus, could be promising in the treatment of oral lichen planus (OLP). The aim of the study was to evaluate the effects of topical hyaluronic acid, compared to placebo, on salivary levels of calprotectin, interleukin-6 (IL-6), and bacteria, as well as clinical and subjective parameters. Fourteen patients with confirmed OLP were included. After random selection, patients started with either 0.2% hyaluronic acid or a placebo gel for 6 weeks. Following a wash-out period, the groups changed the application. Whole saliva, clinical parameters, and questionnaires were evaluated before and after the intervention, as well as after the crossover phase. Salivary calprotectin, IL-6, and inflammation-related bacteria were determined by ELISA and PCR, respectively. There were no significant differences in clinical or subjective outcome parameters, salivary levels of IL-6, calprotectin, or bacteria after the application of hyaluronic acid, compared to placebo. However, only nine patients completed the study, as five out of seven patients starting with placebo were lost to follow-up. Significant effects of HA on inflammatory mediators and clinical parameters in OLP patients could not be proven, although a trend in clinical severity improvement could be observed.

scholarly journals Cytomorphometric analysis of exfoliated cells in oral lichen planus

CytoJournal ◽  
2014 ◽  
Vol 11 ◽  
pp. 3 ◽  
Author(s):  
Chitturi Suryaprakash Ravi Teja ◽  
A. Santha Devy ◽  
R. Madhavan Nirmal ◽  
P. M. Sunil ◽  
M. Deepasree
Keyword(s):  
Lichen Planus ◽  
Oral Lichen Planus ◽  
Control Group ◽  
Exfoliative Cytology ◽  
Normal Tissues ◽  
Exfoliated Cells ◽  
Healthy Mucosa ◽  
Significant Difference ◽  
Malignant Changes ◽  
Oral Lichen

Background: Tumors are distinguished from normal tissues partly by their pronounced variability of cellular and nuclear dimensions. Therefore, such factors may be an indicator to assess whether the cells are malignant or not. Exfoliative cytology is a reliable tool in assessing such changes in the uterine cervix and has been used in the oral cavity also with success. The aims and objectives of the following study were to evaluate the malignant changes by assessing the quantitative parameters such as cytoplasmic diameter, cytoplasmic perimeter and cytoplasmic area (CD, CP, CA) and nuclear diameter, nuclear perimeter and nuclear area (ND, NP, NA) and cytoplasmic to nuclear ratio in the exfoliated cells of various subtypes of oral lichen planus (OLP) using cytomorphometry. Materials and Methods: Oral exfoliated cells from nineteen cases of histologically proven OLP (1 atrophic, 13 reticular, 4 erosive and 1 plaque) and ten controls with healthy mucosa were taken and stained by Feulgen-Rossenback reaction and cytomorphometric analysis was performed using an image analysis software. The parameters taken into account were CD, CP, CA and ND, NP, NA. Furthermore CA/NA was calculated. The parameters were statistically analyzed using the t-test. Results: Cytomorphometric analysis of all the parameters showed no significant difference between the control group and the reticular/plaque subtypes, whereas statistically significant (P < 0.05) differences was obtained between the control group and the atrophic/erosive subtypes group when compared using t-test. Conclusions: The cytomorphometric analysis of OLP shows that erosive/atrophic subtypes of OLP are at more risk and exfoliative cytology and cytomorphometry can be used as a tool to assess the malignant changes.

scholarly journals Dysbiosis of saliva microbiome in patients with oral lichen planus

2020 ◽  
Author(s):  
FeiYan Yu ◽  
QianQian Wang ◽  
Miao Li ◽  
Ya-Hsin Cheng ◽  
Yi-Shing Lisa Cheng ◽  
...  
Keyword(s):  
Lichen Planus ◽  
Relative Abundance ◽  
Oral Lichen Planus ◽  
Disease Process ◽  
Composition Analysis ◽  
Oral Microbiota ◽  
Linear Discriminant ◽  
Α Diversity ◽  
Whole Saliva ◽  
Oral Lichen

Abstract Background : Oral microbiota is not only important for maintaining oral health but also plays a role in oral diseases. However, studies regarding microbiome changes in oral lichen planus(OLP)are very limited. Therefore, the purpose of this study was to identify the characteristic microbial profile in the saliva of OLP patients, with or without erosive lesions, and compare that with recurrent aphthous ulcer (RAU), a common oral immunological disorder that also shows multiple erosive/ulcerative lesions. Methods : Whole saliva samples were collected from 20 patients with OLP (10 each for erosive (E) and non-erosive (NE) groups), 10 patients with RAU (U group), and 10 healthy controls (C). DNA was extracted from the saliva samples, and the 16S rDNA gene V4 hypervariable region was analyzed using Illumina sequencing. Results were assessed with alpha- and beta-diversity, linear discriminant analysis (LDA) effect size, and the data was analyzed by Spearman’s rank correlation,Wilcoxon and Kruskal test. Results: We obtained 4949 operational taxonomic units from the V4 region in all saliva samples. Community composition analysis showed a clear decreased relative abundance of genera Streptococcus and Sphingomonas in saliva from RAU patients when compared to the other three groups. Relative abundance of Lautropia and Gemella were higher in E group,whereas relative abundance of Haemophilus and Neisseria were higher in NE group when compared to C group. Abiotrophia and Oribacterium were higher in OLP (combining E and NE groups), while Eikenella and Aggregatibacter were lower when compared to C group. There was statistically significance in α-diversity between E and RAU groups( p <0.05). Significant differences in β-diversity were detected in bacteria between E and C; NE and C; as well as E and NE groups. Conclusion： We found that salivary microbiome in OLP was significantly different from that found in RAU; and these changes may be related to the underlying disease process rather than presence of ulcerative/erosive lesions clinically. In addition, our findings in bacterial relative abundance in OLP were significantly different from the previously reported findings, which points to the need for further research in salivary microbiome of OLP.

scholarly journals Dysbiosis of saliva microbiome in patients with oral lichen planus

2019 ◽  
Author(s):  
FeiYan Yu ◽  
QianQian Wang ◽  
Miao Li ◽  
Ya-Hsin Cheng ◽  
Yi-Shing Lisa Cheng ◽  
...  
Keyword(s):  
Lichen Planus ◽  
Relative Abundance ◽  
Oral Lichen Planus ◽  
Disease Process ◽  
Composition Analysis ◽  
Oral Microbiota ◽  
Linear Discriminant ◽  
Α Diversity ◽  
Whole Saliva ◽  
Oral Lichen

Abstract Background : Oral microbiota is not only important for maintaining oral health but also plays a role in oral diseases. However, studies regarding microbiome changes in oral lichen planus(OLP)are very limited. Therefore, the purpose of this study was to identify the characteristic microbial profile in the saliva of OLP patients, with or without erosive lesions, and compare that with recurrent aphthous ulcer (RAU), a common oral immunological disorder that also shows multiple erosive/ulcerative lesions. Methods : Whole saliva samples were collected from 20 patients with OLP (10 each for erosive (E) and non-erosive (NE) groups), 10 patients with RAU (U group), and 10 healthy controls (C). DNA was extracted from the saliva samples, and the 16S rDNA gene V4 hypervariable region was analyzed using Illumina sequencing. Results were assessed with alpha- and beta-diversity, linear discriminant analysis (LDA) effect size, and the data was analyzed by Spearman’s rank correlation,Wilcoxon and Kruskal test. Results: We obtained 4949 operational taxonomic units from the V4 region in all saliva samples. Community composition analysis showed a clear decreased relative abundance of genera Streptococcus and Sphingomonas in saliva from RAU patients when compared to the other three groups. Relative abundance of Lautropia and Gemella were higher in E group,whereas relative abundance of Haemophilus and Neisseria were higher in NE group when compared to C group. Abiotrophia and Oribacterium were higher in OLP (combining E and NE groups), while Eikenella and Aggregatibacter were lower when compared to C group. There was statistically significance in α-diversity between E and RAU groups( p <0.05). Significant differences in β-diversity were detected in bacteria between E and C; NE and C; as well as E and NE groups. Conclusion： We found that salivary microbiome in OLP was significantly different from that found in RAU; and these changes may be related to the underlying disease process rather than presence of ulcerative/erosive lesions clinically. In addition, our findings in bacterial relative abundance in OLP were significantly different from the previously reported findings, which points to the need for further research in salivary microbiome of OLP.

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