scholarly journals The Tissue-Engineered Human Psoriatic Skin Substitute: A Valuable In Vitro Model to Identify Genes with Altered Expression in Lesional Psoriasis

2018 ◽  
Vol 19 (10) ◽  
pp. 2923 ◽  
Author(s):  
Geneviève Rioux ◽  
Claudia Pouliot-Bérubé ◽  
Mélissa Simard ◽  
Manel Benhassine ◽  
Jacques Soucy ◽  
...  
Keyword(s):  
Skin Disease ◽  
In Vitro Model ◽  
Expression Profiles ◽  
Gene Profiling ◽  
Skin Substitute ◽  
Psoriatic Skin ◽  
Skin Substitutes ◽  
Altered Expression ◽  
Vitro Model

Psoriasis is a chronic inflammatory skin disease for which no cure has emerged. Its complex etiology requires the development of an in vitro model representative of the pathology. In this study, we exploited gene profiling analyses on microarray in order to characterize and further optimize the production of a human psoriatic skin model representative of this in vivo skin disease. Various skin substitutes were produced by tissue-engineering using biopsies from normal, healthy donors, or from lesional or non-lesional skin samples from patients with psoriasis, and their gene expression profiles were examined by DNA microarray. We demonstrated that more than 3540 and 1088 genes (two-fold change) were deregulated between healthy/lesional and lesional/non-lesional psoriatic substitutes, respectively. Moreover, several genes related to lipid metabolism, such as PLA2G4E and PLA2G4C, were identified as repressed in the lesional substitutes. In conclusion, gene profiling analyses identified a list of deregulated candidate genes associated with various metabolic pathways that may contribute to the progression of psoriasis.

Dynamic In Vitro Model of the Blood–Brain Barrier: Gene Profiling Using cDNA Microarray Analysis

2003 ◽  
pp. 419-434 ◽  
Author(s):  
Matteo Marroni ◽  
Kelly M. Kight ◽  
Mohammed Hossain ◽  
Luca Cucullo ◽  
Shailesh Y. Desai ◽  
...  
Keyword(s):  
Blood Brain Barrier ◽  
Microarray Analysis ◽  
Cdna Microarray ◽  
In Vitro Model ◽  
Gene Profiling ◽  
Brain Barrier ◽  
Cdna Microarray Analysis ◽  
Blood Brain ◽  
Vitro Model

scholarly journals The transcriptional landscape of the murine middle ear epithelium in vitro

2019 ◽  
Author(s):  
Apoorva Mulay ◽  
Md Miraj K Chowdhury ◽  
Cameron James ◽  
Lynne Bingle ◽  
Colin D Bingle
Keyword(s):  
Gene Expression ◽  
Middle Ear ◽  
In Vitro Model ◽  
Expression Profiles ◽  
Gene Expression Profiles ◽  
Inflammatory Cells ◽  
Differentially Expressed ◽  
Vitro Model ◽  
Transcriptional Landscape

AbstractOtitis media (OM) is the most common paediatric disease and leads to significant morbidity. Although understanding of underlying disease mechanisms is hampered by complex pathophysiology, it is clear that epithelial abnormalities underpin the disease. The mechanisms underpinning epithelial remodelling in OM remain unclear. We recently described a novel in vitro model of mouse middle ear epithelial cells (mMEECs) that undergoes mucociliary differentiation into the varied epithelial cell populations seen in the middle ear cavity. We now describe genome wide gene expression profiles of mMEECs as they undergo differentiation. We compared the gene expression profiles of original (uncultured) middle ear cells, confluent cultures of undifferentiated cells (day 0 of ALI) and cells that had been differentiated for 7 days at an ALI. >5000 genes were differentially expressed among the three groups of cells. Approximately 4000 genes were differentially expressed between the original cells and day 0 of ALI culture. The original cell population was shown to contain a mix of cell types, including contaminating inflammatory cells that were lost on culture. Approximately 500 genes were upregulated during ALI induced differentiation. These included some secretory genes and some enzymes but most were associated with the process of ciliogenesis. Our in vitro model of differentiated murine middle ear epithelium exhibits a transcriptional profile consistent with the mucociliary epithelium seen within the middle ear. Knowledge of the transcriptional landscape of this epithelium will provide a basis for understanding the phenotypic changes seen in murine models of OM.

scholarly journals An in Vitro Model of Differentiation of Memory B Cells Into Plasmablasts and Plasma Cells Including Detailed Phenotypic and Molecular Characterization.

Blood ◽  
2009 ◽  
Vol 114 (22) ◽  
pp. 1636-1636
Author(s):  
Michel Jourdan ◽  
Anouk Caraux ◽  
John De Vos ◽  
Geneviève Fiol ◽  
Marion Larroque ◽  
...  
Keyword(s):  
B Cells ◽  
In Vitro Model ◽  
Plasma Cells ◽  
Conflicts Of Interest ◽  
Expression Profiles ◽  
Gene Expression Profiles ◽  
Memory B Cells ◽  
Vitro Model ◽  
Intermediate Cells

Abstract Abstract 1636 Poster Board I-662 Human plasma cells (PCs) and their precursors play an essential role in humoral immune response, but are rare and difficult to harvest. We report here i) the generation of human syndecan-1+ and immunoglobulin secreting PCs starting from memory B cells (MBCs) in a 3-step- and 10-day (D) culture, including a 6-fold cell amplification. ii) We report the detailed phenotypic and Affymetrix gene expression profiles of these in vitro PCs as well as of intermediate cells - activated B cells (actBCs) and plasmablasts (PBs) - compared to MBCs and bone marrow PCs, which is accessible through an open web ATLAS (http://amazonia.transcriptome.eu/). iii) We show this B cell to PC differentiation to involve IRF4 and AICDA expression in D4 actBCs, decrease of PAX5 and BCL6 expressions and increase in PRDM1 and XBP1 expressions in D7 PBs and D10 PCs. It involves downregulation of genes controlled by Pax5, induction of genes controlled by Blimp-1 and XBP1 (unfold protein response). iv) The phenotype of D10 PCs resembles that of peripheral blood PCs detected after immunization of healthy donors. This in vitro model will facilitate further studies in PC biology. It will likewise be helpful to study plasma-cell dyscrasias, including Multiple Myeloma. Disclosures No relevant conflicts of interest to declare.

scholarly journals An in vitro model of differentiation of memory B cells into plasmablasts and plasma cells including detailed phenotypic and molecular characterization

Blood ◽  
2009 ◽  
Vol 114 (25) ◽  
pp. 5173-5181 ◽  
Author(s):  
Michel Jourdan ◽  
Anouk Caraux ◽  
John De Vos ◽  
Geneviève Fiol ◽  
Marion Larroque ◽  
...  
Keyword(s):  
B Cells ◽  
In Vitro Model ◽  
Plasma Cells ◽  
Expression Profiles ◽  
Gene Expression Profiles ◽  
Memory B Cells ◽  
Vitro Model ◽  
Intermediate Cells ◽  
Activated B Cells

Abstract Human plasma cells (PCs) and their precursors play an essential role in humoral immune response but are rare and difficult to harvest. We report the generation of human syndecan-1+ and immunoglobulin secreting PCs starting from memory B cells in a 3-step and 10-day (D) culture, including a 6-fold cell amplification. We report the detailed phenotypic and Affymetrix gene expression profiles of these in vitro PCs as well as of intermediate cells (activated B cells and plasmablasts) compared with memory B cells and bone marrow PCs, which is accessible through an open web ATLAS (http://amazonia.transcriptome.eu/). We show this B cell–to-PC differentiation to involve IRF4 and AICDA expressions in D4 activated B cells, decrease of PAX5 and BCL6 expressions, and increase in PRDM1 and XBP1 expressions in D7 plasmablasts and D10 PCs. It involves down-regulation of genes controlled by Pax5 and induction of genes controlled by Blimp-1 and XBP1 (unfold protein response). The detailed phenotype of D10 PCs resembles that of peripheral blood PCs detected after immunization of healthy donors. This in vitro model will facilitate further studies in PC biology. It will likewise be helpful to study PC dyscrasias, including multiple myeloma.

scholarly journals Effects of functional pasta ingredients on different gut microbiota as revealed by TIM-2 in vitro model of the proximal colon

2019 ◽  
Vol 10 (3) ◽  
pp. 301-313
Author(s):  
A. Martina ◽  
G.E. Felis ◽  
M. Corradi ◽  
C. Maffeis ◽  
S. Torriani ◽  
...  
Keyword(s):  
Gut Microbiota ◽  
In Vitro Model ◽  
Bacillus Coagulans ◽  
Proximal Colon ◽  
Gene Profiling ◽  
Rrna Gene ◽  
Dietary Interventions ◽  
Vitro Model ◽  
Microbial Groups

Diet-related modulation of gut microbiota and its metabolic activity represents an intriguing research context, particularly in the case of disorders related to imbalances in gut microbial communities. We here explored the effects of Bacillus coagulans GBI-30, 6086 (BC30), β-glucans, and innovative whole-grain pastas, with or without these functional ingredients, on gut microbiota from three groups of children, presenting different susceptibility to type 1 diabetes, by using the well-controlled TNO in vitro model of the proximal colon (TIM-2). Short- and branched-chain fatty acids production and microbiota composition were assessed by means of gas chromatography and 16S rRNA gene profiling, respectively. In most cases, in vitro dietary interventions caused microbiota-dependent modulations as a result of intergroup variability, but also specific changes in microbial groups were shared between the three microbiotas, highlighting specific diet-microbial taxa connections.

Effects of low bisphenol A concentration on protein expression profiles in an in vitro model of non-alcoholic fatty liver disease

2018 ◽  
Vol 14 (1) ◽  
pp. 61-70 ◽  
Author(s):  
Peerut Chienwichai ◽  
Supachai Topanurak ◽  
Onrapak Reamtong ◽  
Usa Boonyuen ◽  
Suwalee Worakhunpiset ◽  
...  
Keyword(s):  
Liver Disease ◽  
Bisphenol A ◽  
Fatty Liver Disease ◽  
In Vitro Model ◽  
Expression Profiles ◽  
Alcoholic Fatty Liver ◽  
Vitro Model ◽  
Protein Expression Profiles ◽  
Alcoholic Fatty Liver Disease

scholarly journals An In Vitro Model of Mast Cell Recruitment and Activation by Breast Cancer Cells Supports Anti-Tumoral Responses

2020 ◽  
Vol 21 (15) ◽  
pp. 5293
Author(s):  
Angélica Aponte-López ◽  
Jennifer Enciso ◽  
Samira Muñoz-Cruz ◽  
Ezequiel M. Fuentes-Pananá
Keyword(s):  
Breast Cancer ◽  
Breast Cancer Cells ◽  
In Vitro Model ◽  
Tumor Control ◽  
Altered Expression ◽  
Cell Recruitment ◽  
Control Tumor Growth ◽  
Control Tumor ◽  
Vitro Model

Breast cancer (BrC) affects millions of women yearly. Mast cells (MCs) are common components of breast tumors with documented agonistic and antagonistic roles in tumor progression. Understanding the participation of MCs in BrC may lead to new therapies to control tumor growth. In this study, we looked into mechanistic models of MC responses triggered by BrC cells (BrCC), assessing both early degranulation and late transcriptional activities. We used aggressive and non-aggressive BrCC to model the progressive staging of the disease over HMC1 and LAD-2 human MC lines. We found that both MC lines were chemoattracted by all BrCC, but their activation was preferentially induced by aggressive lines, finding differences in their active transcriptional programs, both at basal level and after stimulation. Among those genes with altered expression were down-regulated SPP1, PDCD1, IL17A and TGFB1 and up-regulated KITLG and IFNG. A low expression of SPP1 and a high expression of KITLG and IFNG were associated with increased overall survival of BrC patients from public databases. The set of altered genes is more often associated with tumor stromas enriched with anti-tumoral signals, suggesting that MCs may participate in tumor control.

scholarly journals Biomimetic In Vitro Model of Cell Infiltration into Skin Scaffolds for Pre-Screening and Testing of Biomaterial-Based Therapies

Cells ◽  
2019 ◽  
Vol 8 (8) ◽  
pp. 917
Author(s):  
Rafael Ballesteros-Cillero ◽  
Evan Davison-Kotler ◽  
Nupur Kohli ◽  
William S. Marshall ◽  
Elena García-Gareta
Keyword(s):  
Wound Healing ◽  
In Vitro Model ◽  
Chronic Wounds ◽  
Healing Process ◽  
Dermal Fibroblasts ◽  
Surrounding Tissue ◽  
Cell Infiltration ◽  
Skin Substitutes ◽  
Vitro Model

Due to great clinical need, research where different biomaterials are tested as 3D scaffolds for skin tissue engineering has increased. In vitro studies use a cell suspension that is simply pipetted onto the material and cultured until the cells migrate and proliferate within the 3D scaffold, which does not mimic the in vivo reality. Our aim was to engineer a novel biomimetic in vitro model that mimics the natural cell infiltration process occurring in wound healing, thus offering a realistic approach when pre-screening and testing new skin substitutes. Our model consists of porous membrane cell culture inserts coated with gelatin and seeded with human dermal fibroblasts, inside which two different commercially available dermal substitutes were placed. Several features relevant to the wound healing process (matrix contraction, cell infiltration and proliferation, integration of the biomaterial with the surrounding tissue, and secretion of exogenous cytokines and growth factors) were evaluated. Our results showed that cells spontaneously infiltrate the materials and that our engineered model is able to induce and detect subtle differences between different biomaterials. The model allows for room for improvements or “adds-on” and miniaturization and can contribute to the development of functional and efficient skin substitutes for burns and chronic wounds.

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