scholarly journals Transcriptomic Analysis of Planarians under Simulated Microgravity or 8 g Demonstrates That Alteration of Gravity Induces Genomic and Cellular Alterations That Could Facilitate Tumoral Transformation

2019 ◽  
Vol 20 (3) ◽  
pp. 720
Author(s):  
Nídia de Sousa ◽  
Gustavo Rodriguez-Esteban ◽  
Ivan Colagè ◽  
Paolo D’Ambrosio ◽  
Jack van Loon ◽  
...  
Keyword(s):  
Simulated Microgravity ◽  
Model System ◽  
Transcriptomic Analysis ◽  
Hippo Signaling ◽  
Random Positioning Machine ◽  
Transcriptomic Data ◽  
Earth Environment ◽  
Ideal System ◽  
The World ◽  
Cellular Alterations

The possibility of humans to live outside of Earth on another planet has attracted the attention of numerous scientists around the world. One of the greatest difficulties is that humans cannot live in an extra-Earth environment without proper equipment. In addition, the consequences of chronic gravity alterations in human body are not known. Here, we used planarians as a model system to test how gravity fluctuations could affect complex organisms. Planarians are an ideal system, since they can regenerate any missing part and they are continuously renewing their tissues. We performed a transcriptomic analysis of animals submitted to simulated microgravity (Random Positioning Machine, RPM) (s-µg) and hypergravity (8 g), and we observed that the transcriptional levels of several genes are affected. Surprisingly, we found the major differences in the s-µg group. The results obtained in the transcriptomic analysis were validated, demonstrating that our transcriptomic data is reliable. We also found that, in a sensitive environment, as under Hippo signaling silencing, gravity fluctuations potentiate the increase in cell proliferation. Our data revealed that changes in gravity severely affect genetic transcription and that these alterations potentiate molecular disorders that could promote the development of multiple diseases such as cancer.

scholarly journals Planarians Sense Simulated Microgravity and Hypergravity

2014 ◽  
Vol 2014 ◽  
pp. 1-10 ◽  
Author(s):  
Teresa Adell ◽  
Emili Saló ◽  
Jack J. W. A. van Loon ◽  
Gennaro Auletta
Keyword(s):  
Cell Biology ◽  
Simulated Microgravity ◽  
Large Diameter ◽  
Model System ◽  
Stem Cell Biology ◽  
Altered Gravity ◽  
Random Positioning Machine ◽  
Planarian Regeneration ◽  
Regenerative Ability ◽  
Classical Subject

Planarians are flatworms, which belong to the phylumPlatyhelminthes.They have been a classical subject of study due to their amazing regenerative ability, which relies on the existence of adult totipotent stem cells. Nowadays they are an emerging model system in the field of developmental, regenerative, and stem cell biology. In this study we analyze the effect of a simulated microgravity and a hypergravity environment during the process of planarian regeneration and embryogenesis. We demonstrate that simulated microgravity by means of the random positioning machine (RPM) set at a speed of 60 °/s but not at 10 °/s produces the dead of planarians. Under hypergravity of 3 g and 4 g in a large diameter centrifuge (LDC) planarians can regenerate missing tissues, although a decrease in the proliferation rate is observed. Under 8 g hypergravity small planarian fragments are not able to regenerate. Moreover, we found an effect of gravity alterations in the rate of planarian scission, which is its asexual mode of reproduction. No apparent effects of altered gravity were found during the embryonic development.

PERANCANGAN ARSITEKTUR RUANG LIMINAL ANTARA SEBUAH DUALISME (BERTANI DAN MELAUT)

2021 ◽  
Vol 3 (1) ◽  
pp. 1087
Author(s):  
Michael Gideon Josian ◽  
Maria Veronica Gandha
Keyword(s):  
General Public ◽  
Point Of View ◽  
Common Space ◽  
Special Character ◽  
Ideal System ◽  
The World ◽  
The Future ◽  
Letting Go

The future of dwelling has a very board context and will continue to be discussed, it is possible that the discussions about “dwelling” is come from the environment of farming and fishing. Things that are not much cared for but still have a role in the survival of the world. Therefore this matter will be discussed using the role of architecture as space, to be able to create an ideal system by paying attention to the quality of farming and fishing for the future, and leaving a trace or memory to be able to carry messages for the future. Talking about the future of an interaction that occurs between the general public and farmers and fishermen, especially considering that farmers and fishermen themselves can be compared to two different poles, a liminal space is needed, which may already exist indirectly in the environment. By letting go of individual egos and emphasizing ego to the point of view of farmers and fishermen. To present a common space, or a place that contains a special character of a city that contains a message for the future. Keywords:  dualism; hope; liminal; trace;  Abstrak Masa depan cara berhuni memiliki konteks yang sangat luas dan akan terus diperbincangkan. Tidak menutup kemungkinan datang dari pembahasan mengenai cara berhuni dengan bertani dan melaut. Hal yang tidak banyak dipedulikan tetapi tetap memiliki peran dalam kelangsungan dunia. Oleh karena itu, masa depan berhuni ini akan dibahas dengan menggunakan peran arsitektur sebagai ruang, untuk dapat menciptakan sistem yang ideal dengan memperhatikan kualitas bertani dan melaut bagi masa depan, dan meninggalkan sebuah jejak atau kenangan untuk dapat membawa pesan bagi masa depan. Berbicara mengenai masa depan dari sebuah interaksi yang terjadi antara masyarakat umum dengan para petani dan nelayan, apalagi mengingat para petani dan nelayan itu sendiri dapat diibaratkan berada pada kedua kutub yang berbeda, maka dibutuhkanlah sebuah ruang liminal, yang mungkin sudah hadir secara tidak langsung pada lingkungan masyarakat. Dengan cara melepaskan ego individual dan menekankan ego kepada sudut pandang para petani dan nelayan. Untuk menghadirkan sebuah ruang bersama, atau sebuah tempat yang mengandung sebuah karakter tersendiri dari sebuah kota yang berisi pesan bagi masa depan.

Simulated microgravity using the Random Positioning Machine inhibits differentiation and alters gene expression profiles of 2T3 preosteoblasts

2005 ◽  
Vol 288 (6) ◽  
pp. C1211-C1221 ◽  
Author(s):  
Steven J. Pardo ◽  
Mamta J. Patel ◽  
Michelle C. Sykes ◽  
Manu O. Platt ◽  
Nolan L. Boyd ◽  
...  
Keyword(s):  
Gene Expression ◽  
Alkaline Phosphatase ◽  
Bone Loss ◽  
Simulated Microgravity ◽  
Expression Profiles ◽  
Gene Expression Profiles ◽  
Underlying Mechanism ◽  
Bone Biology ◽  
Random Positioning Machine

Exposure to microgravity causes bone loss in humans, and the underlying mechanism is thought to be at least partially due to a decrease in bone formation by osteoblasts. In the present study, we examined the hypothesis that microgravity changes osteoblast gene expression profiles, resulting in bone loss. For this study, we developed an in vitro system that simulates microgravity using the Random Positioning Machine (RPM) to study the effects of microgravity on 2T3 preosteoblast cells grown in gas-permeable culture disks. Exposure of 2T3 cells to simulated microgravity using the RPM for up to 9 days significantly inhibited alkaline phosphatase activity, recapitulating a bone loss response that occurs in real microgravity conditions without altering cell proliferation and shape. Next, we performed DNA microarray analysis to determine the gene expression profile of 2T3 cells exposed to 3 days of simulated microgravity. Among 10,000 genes examined using the microarray, 88 were downregulated and 52 were upregulated significantly more than twofold using simulated microgravity compared with the static 1-g condition. We then verified the microarray data for some of the genes relevant in bone biology using real-time PCR assays and immunoblotting. We confirmed that microgravity downregulated levels of alkaline phosphatase, runt-related transcription factor 2, osteomodulin, and parathyroid hormone receptor 1 mRNA; upregulated cathepsin K mRNA; and did not significantly affect bone morphogenic protein 4 and cystatin C protein levels. The identification of gravisensitive genes provides useful insight that may lead to further hypotheses regarding their roles in not only microgravity-induced bone loss but also the general patient population with similar pathological conditions, such as osteoporosis.

scholarly journals Hiliatic theories in the context of Protestant eschatology (exegetical-comparative analysis

2006 ◽  
pp. 70-85
Author(s):  
Vitaliy I. Docush
Keyword(s):  
Comparative Analysis ◽  
Drug Addiction ◽  
Kingdom Of God ◽  
The State ◽  
Existing Problems ◽  
Global Character ◽  
The Earth ◽  
Ideal System ◽  
The World ◽  
Qualitative Characteristics

At the intersection of the second and third millennia in connection with the natural (destruction of the state of the earth, water and atmosphere) and social (alcoholism, drug addiction, immoralism, extremism, wars, etc.) cataclysms that are taking on a global character, the eschatological prophecies about the end of the world have intensified the coming of the millennial Kingdom of God. In contrast to the existing problems, the Kingdom of God is offered as an ideal system of government with such qualitative characteristics as equality, justice, material and spiritual completeness.

scholarly journals Spatial Meta-transcriptomes of human and murine intestines

2021 ◽  
Author(s):  
Lin Lyu ◽  
Ru Feng ◽  
Xue Li ◽  
Xiaofei Yu ◽  
GuoQiang Chen ◽  
...  
Keyword(s):  
Simultaneous Analysis ◽  
Transcriptomic Analysis ◽  
Microbial Abundance ◽  
Proof Of Concept ◽  
Analysis Pipeline ◽  
Experimental Optimization ◽  
Transcriptomic Data ◽  
Microbial Distribution ◽  
The Way

We developed an analysis pipeline that can extract microbial sequences from Spatial Transcriptomic data and assign taxonomic labels to them, generating a spatial microbial abundance matrix in addition to the default host expression one, enabling simultaneous analysis of host expression and microbial distribution. We applied it on both human and murine intestinal datasets and validated the spatial microbial abundance information with alternative assays. Finally, we present a few biological insights that can be gained from this novel data. In summary, this proof of concept work demonstrated the feasibility of Spatial Meta-transcriptomic analysis, and pave the way for future experimental optimization.

scholarly journals Effects of altered gravity induced by clinorotation on the cholinesterase activity of the non-sentient model Paramecium primaurelia (Protozoa)

2018 ◽  
Vol 91 (1) ◽  
Author(s):  
Francesca Sardi ◽  
Martina Rossi ◽  
Sara Ferrando ◽  
Maria Angela Masini ◽  
Federico Biggi ◽  
...  
Keyword(s):  
Simulated Microgravity ◽  
Cholinesterase Activity ◽  
Developmental Cycle ◽  
Unicellular Organism ◽  
Altered Gravity ◽  
Random Positioning Machine ◽  
Earth Gravity ◽  
Microgravity Conditions ◽  
Living Organisms ◽  
Chemical Mediators

Compounds known as chemical mediators, including acetylcholine, have been found not only in humans and animals, but also in living organisms, like protozoa, which lack nervous system. In Paramecium primaurelia has been described a cholinergic system, which is proven to play an important role in cell-cell interactions during its developmental cycle. In our work we investigated the effects of exposure to simulated microgravity (3D Random Positioning Machine, 56 rpm, 10-6 g) on the cholinesterase activity of the eukaryote unicellular-organism alternative-model P. primaurelia. Our results show that the exposure of P. primaurelia to microgravity for 6 h, 24 h, 48 h affects the localization and the amount of cholinesterase activity compared to cells grown under Earth gravity conditions (1 g). However, these effects are transient since P. primaurelia restores its normal cholinesterase activity after 72 h under microgravity conditions, as well as cells exposed up to 72 h to microgravity and then placed under terrestrial gravity for 48 h.

Molecular Ecology of Class 1 Integrons in Patagonia as Model System for Understanding the Rise of Antibiotic Resistance Isolates Around the World

2016 ◽  
pp. 111-125 ◽  
Author(s):  
Verónica Elizabeth Álvarez ◽  
María Paula Quiroga ◽  
Gabriel Alejandro Castro ◽  
Marcelo Hernán Cassini ◽  
Daniela Centrón
Keyword(s):  
Antibiotic Resistance ◽  
Molecular Ecology ◽  
Model System ◽  
Class 1 Integrons ◽  
The World ◽  
Class 1

scholarly journals Alterations in the activity and sleep of Drosophila melanogaster under simulated microgravity

2021 ◽  
Vol 7 (1) ◽  
Author(s):  
Hongying Zhang ◽  
Yahong Wang ◽  
Ziyan Zhang ◽  
Lu Zhang ◽  
Chao Tang ◽  
...  
Keyword(s):  
Drosophila Melanogaster ◽  
Simulated Microgravity ◽  
Clock Genes ◽  
Fruit Flies ◽  
Light Conditions ◽  
Random Positioning Machine ◽  
Space Life Science ◽  
Waking Up ◽  
Different Strains ◽  
Constant Dark

AbstractThis study aimed to investigate alterations in the activity and sleep of Drosophila melanogaster under simulated microgravity, which was implemented through the random positioning machine, while different light conditions (normal photoperiod and constant dark) were set. Fruit flies of different strains and sexes were treated for 3 days, and activity and sleep were monitored using the Drosophila Activity Monitoring System. After 3 days of treatment, fruit flies were sampled to detect the relative expression levels of the major clock genes and some neurotransmitter-related genes. The results showed that for the normal photoperiod (LD) condition, the activity increased and sleep decreased under simulated microgravity, while for the constant dark (DD) condition, the activity and sleep rhythms appeared disordered and the activity increased, thus decreasing the likelihood of waking up during the day. Light conditions, strains, and sexes, individually or in combination, had impacts on the simulated microgravity effects on behaviors. The clock genes and neurotransmitter-related genes had different degrees of response among sexes and strains, although the overall changes were slight. The results indicated that the normal photoperiod could ease the effects of simulated microgravity on fruit flies’ activity and sleep and possible unidentified pathways involved in the regulatory mechanism need further exploration. This study is expected to provide ideas and references for studying the effects of microgravity on space life science.

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