scholarly journals Transcriptome Profile Analysis of Winter Rapeseed (Brassica napus L.) in Response to Freezing Stress, Reveal Potentially Connected Events to Freezing Stress

2019 ◽  
Vol 20 (11) ◽  
pp. 2771 ◽  
Author(s):  
Yuanyuan Pu ◽  
Lijun Liu ◽  
Junyan Wu ◽  
Yuhong Zhao ◽  
Jing Bai ◽  
...  
Keyword(s):  
Signal Transduction ◽  
Brassica Napus ◽  
Molecular Mechanism ◽  
Stress Responses ◽  
Soluble Sugars ◽  
Enrichment Analysis ◽  
Freezing Stress ◽  
Oilseed Crop ◽  
Brassica Napus L ◽  
Winter Rapeseed

Winter rapeseed is not only an important oilseed crop, but also a winter cover crop in Northern China, where its production was severely limited by freezing stress. As an overwinter crop, the production is severely limited by freezing stress. Therefore, understanding the physiological and molecular mechanism of winter rapeseed (Brassica napus L.) in freezing stress responses becomes essential for the improvement and development of freezing-tolerant varieties of Brassica napus. In this study, morphological, physiological, ultrastructure and transcriptome changes in the Brassica napus line “2016TS(G)10” (freezing-tolerance line) that was exposed to –2 °C for 0 h, 1 h, 3 h and 24 h were characterized. The results showed that freezing stress caused seedling dehydration, and chloroplast dilation and degradation. The content of malondialdehyde (MDA), proline, soluble protein and soluble sugars were increased, as well as the relative electrolyte leakage (REL) which was significantly increased at frozen 24 h. Subsequently, RNA-seq analysis revealed a total of 98,672 UniGenes that were annotated in Brassica napus and 3905 UniGenes were identified as differentially expressed genes after being exposed to freezing stress. Among these genes, 2312 (59.21%) were up-regulated and 1593 (40.79%) were down-regulated. Most of these DEGs were significantly annotated in the carbohydrates and energy metabolism, signal transduction, amino acid metabolism and translation. Most of the up-regulated DEGs were especially enriched in plant hormone signal transduction, starch and sucrose metabolism pathways. Transcription factor enrichment analysis showed that the AP2/ERF, WRKY and MYB families were also significantly changed. Furthermore, 20 DEGs were selected to validate the transcriptome profiles via quantitative real-time PCR (qRT-PCR). In conclusion, the results provide an overall view of the dynamic changes in physiology and insights into the molecular regulation mechanisms of winter Brassica napus in response to freezing treatment, expanding our understanding on the complex molecular mechanism in plant response to freezing stress.

scholarly journals iTRAQ-based quantitative proteome analysis insights into cold stress of Winter Rapeseed (Brassica rapa L.) grown in the field

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Zaoxia Niu ◽  
Lijun Liu ◽  
Yuanyuan Pu ◽  
Li Ma ◽  
Junyan Wu ◽  
...  
Keyword(s):  
Cold Stress ◽  
Brassica Rapa ◽  
Molecular Mechanisms ◽  
Glutathione Transferase ◽  
Soluble Sugars ◽  
Freezing Stress ◽  
Transferase Activity ◽  
Oilseed Crop ◽  
Winter Rapeseed ◽  
Collar Diameter

AbstractWinter rapeseed (Brassica rapa L.) is a major oilseed crop in Northern China, where its production was severely affected by chilling and freezing stress. However, not much is known about the role of differentially accumulated proteins (DAPs) during the chilling and freezing stress. In this study, isobaric tag for relative and absolute quantification (iTRAQ) technology was performed to identify DAPs under freezing stress. To explore the molecular mechanisms of cold stress tolerance at the cellular and protein levels, the morphological and physiological differences in the shoot apical meristem (SAM) of two winter rapeseed varieties, Longyou 7 (cold-tolerant) and Lenox (cold-sensitive), were explored in field-grown plants. Compared to Lenox, Longyou 7 had a lower SAM height and higher collar diameter. The level of malondialdehyde (MDA) and indole-3-acetic acid (IAA) content was also decreased. Simultaneously, the soluble sugars (SS) content, superoxide dismutase (SOD) activity, peroxidase (POD) activity, soluble protein (SP) content, and collar diameter were increased in Longyou 7 as compared to Lenox. A total of 6330 proteins were identified. Among this, 98, 107, 183 and 111 DAPs were expressed in L7 CK/Le CK, L7 d/Le d, Le d/Le CK and L7 d/L7 CK, respectively. Quantitative real-time PCR (RT-qPCR) analysis of the coding genes for seventeen randomly selected DAPs was performed for validation. These DAPs were identified based on gene ontology enrichment analysis, which revealed that glutathione transferase activity, carbohydrate-binding, glutathione binding, metabolic process, and IAA response were closely associated with the cold stress response. In addition, some cold-induced proteins, such as glutathione S-transferase phi 2(GSTF2), might play an essential role during cold acclimation in the SAM of Brassica rapa. The present study provides valuable information on the involvement of DAPs during cold stress responses in Brassica rapa L, and hence could be used for breeding experiments.

scholarly journals Comparative Transcriptomics and Proteomics Analyses of Leaves Reveals a Freezing Stress-Responsive Molecular Network in Winter Rapeseed (Brassica rapa L.)

2021 ◽  
Vol 12 ◽  
Author(s):  
Jiaping Wei ◽  
Guoqiang Zheng ◽  
Xingwang Yu ◽  
Sushuang Liu ◽  
Xiaoyun Dong ◽  
...  
Keyword(s):  
Signal Transduction ◽  
Freezing Tolerance ◽  
Crop Production ◽  
Enrichment Analysis ◽  
Freezing Temperature ◽  
Northwest China ◽  
Freezing Stress ◽  
Glutathione Metabolism ◽  
Cell Stability ◽  
Winter Rapeseed

Winter rapeseed is susceptible to low temperature during winter in Northwest China, which could lead to a severe reduction of crop production. The freezing temperature could stress the whole plant, especially the leaf, and ultimately harm the survival rate of winter rapeseed. However, the molecular mechanism underlying freezing tolerance is still unclear in winter rapeseed. In this study, a comprehensive investigation of winter rapeseed freezing tolerance was conducted at the levels of transcript, protein, and physiology and biochemistry, using a pair of freezing-sensitive and freezing-resistant cultivars NQF24 and 17NTS57. There were 4,319 unique differentially expressed genes (DEGs) and 137 unique differentially abundant proteins (DAPs) between two cultivars identified in leaf under freezing stress. Function enrichment analysis showed that most of the enriched DEGs and DAPs were involved in plant hormone signal transduction, alpha-linolenic/linoleic acid metabolism, peroxisome, glutathione metabolism, fatty acid degradation, and secondary metabolite biosynthesis pathways. Based on our findings, it was speculated that freezing tolerance formation is caused by increased signal transduction, enhanced biosynthesis of protein, secondary metabolites, and plant hormones, elevated energy supply, greater reactive oxygen species scavenging, and lower lipid peroxidation as well as stronger cell stability in leaf under freezing stress. These results provide a comprehensive profile of leaf response under freezing stress, which have potential to be used as selection indicators of breeding programs to improve freezing tolerance in rapeseed.

scholarly journals Extent and structure of linkage disequilibrium in canola quality winter rapeseed (Brassica napus L.)

2009 ◽  
Vol 120 (5) ◽  
pp. 921-931 ◽  
Author(s):  
Wolfgang Ecke ◽  
Rosemarie Clemens ◽  
Nora Honsdorf ◽  
Heiko C. Becker
Keyword(s):  
Linkage Disequilibrium ◽  
Brassica Napus ◽  
Brassica Napus L ◽  
Winter Rapeseed ◽  
Canola Quality

scholarly journals Zinc-lysine Supplementation Mitigates Oxidative Stress in Rapeseed (Brassica napus L.) by Preventing Phytotoxicity of Chromium, When Irrigated with Tannery Wastewater

Plants ◽  
2020 ◽  
Vol 9 (9) ◽  
pp. 1145 ◽  
Author(s):  
Ihsan Elahi Zaheer ◽  
Shafaqat Ali ◽  
Muhammad Hamzah Saleem ◽  
Muhammad Arslan Ashraf ◽  
Qurban Ali ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Brassica Napus ◽  
Plant Growth ◽  
Tannery Wastewater ◽  
Oilseed Crop ◽  
Toxic Heavy Metals ◽  
Gas Exchange Parameters ◽  
Brassica Napus L ◽  
Cr Content ◽  
Cr Toxicity

Contamination of soil and water with metals and metalloids is one of the most serious problems worldwide due to a lack of a healthy diet and food scarcity. Moreover, the cultivation of oilseed crops such as rapeseed (Brassica napus L.) with tannery wastewater could contain a large amount of toxic heavy metals [e.g., chromium (Cr)], which ultimately reduce its yield and directly influence oilseed quality. To overcome Cr toxicity in B. napus, a pot experiment was conducted to enhance plant growth and biomass by using newly introduced role of micronutrient-amino chelates [Zinc-lysine (Zn-lys)], which was irrigated with different levels [0% (control), 33%, 66%, and 100%] of tannery wastewater. According to the results of present findings, very high content of Cr in the wastewater directly affected plant growth and composition as well as gas exchange parameters, while boosting up the production of reactive oxygen species (ROS) and induced oxidative damage in the roots and leaves of B. napus. However, activities of antioxidants initially increased (33% of wastewater), but further addition of tannery wastewater in the soil caused a decrease in antioxidant enzymes, which also manifested by Zn content, while the conscious addition of wastewater significantly increased Cr content in the roots and shoots of B. napus. To reduce Cr toxicity in B. napus plants, exogenous supplementation of Zn-lys (10 mg/L) plays an effective role in increasing morpho-physiological attributes of B. napus and also reduces the oxidative stress in the roots and leaves of the oilseed crop (B. napus). Enhancement in different growth attributes was directly linked with increased in antioxidative enzymes while decreased uptake and accumulation of Cr content in B. napus when cultivated in wastewater with the application of Zn-lys. Zn-lys, therefore, plays a protective role in reducing the Cr toxicity of B. napus through an increase in plant growth and lowering of Cr uptake in various plant organs. However, further studies at field levels are required to explore the mechanisms of Zn–lys mediated reduction of Cr and possibly other heavy metal toxicity in plants.

scholarly journals DOUBLE CROPPING WINTER RAPESEED AND GRAIN SORGHUM

1986 ◽  
Vol 66 (3) ◽  
pp. 425-430 ◽  
Author(s):  
R. R. DUNCAN ◽  
C. S. HOVELAND
Keyword(s):  
Glycine Max ◽  
Brassica Napus ◽  
Cropping System ◽  
Grain Sorghum ◽  
Winter Rape ◽  
Brassica Napus L ◽  
Double Cropping ◽  
Glycine Max L ◽  
Winter Rapeseed ◽  
Sorghum Bicolor L

Since rainfall generally exceeds 500 mm during the winter months and soil erosion is a problem in the southern U.S.A., a cover crop is essential to reduce soil losses. Continuous cultivation of a crop on the same land also provides the opportunity for pest problems to develop. A winter rapeseed (Brassica napus L.)-grain sorghum (Sorghum bicolor (L). Moench.) double-cropping system was investigated in the field from 1981 to 1984. Conventional tillage (CT) and no-till (NT) systems were used for both crops. Gullivar rape was planted in October and harvested in June. Winter rapeseed yields averaged 2.87 t ha−1 on CT plots in 1982. Averaged over 1983 and 1984, rape yields were 1.66 and 0.73 t ha−1 on CT and NT plots, respectively. However, the seeding method biased the rapeseed performance. Problems were encountered with winter weed control and pod shattering in the winter rape plots. Two sorghum hybrids, R. C. Young Oro Txtra and Funks G-550, were planted in June and harvested in October. Grain yields were not significantly different between hybrids (Oro 5.69 t ha−1, Funk 5.67 t ha−1) or between tillage systems (CT 5.59 t ha−1, NT 5.78 t ha−1). Grain yield differences were noted for year × hybrid × tillage interactions. Rape was a weed problem in the sorghum plots. The winter rape-sorghum double-cropping system offers an alternative to current winter small grains-sorghum/soybean (Glycine max (L.) Merr.) or winter annual legume-sorghum/soybean (Glycine max (L.) Merr.) systems; however, markets for the winter rapeseed and production economic analyses are needed prior to adoption of the system. Additional winter rapeseed research is needed to evaluate yields properly under NT conditions.Key words: Sorghum bicolor (L.) Moench, Brassica napus L., conservation tillage, multiple cropping, sorghum, rapeseed

ADAPTATION OF WINTER RAPESEED IN ONTARIO

1987 ◽  
Vol 67 (3) ◽  
pp. 675-684 ◽  
Author(s):  
G. C. BEAULIEU ◽  
D. J. HUME
Keyword(s):  
Brassica Napus ◽  
Field Trials ◽  
Winter Survival ◽  
First Year ◽  
Brassica Napus L ◽  
Northern Ontario ◽  
Cold Temperatures ◽  
The North ◽  
Winter Snow ◽  
Winter Rapeseed

In order to determine regions of adaptation of winter rapeseed (Brassica napus L.), field trials were planted at about 30 locations in Ontario in 1981 and 1982. Four cultivars were tested each year. Winter survival was poor in northern Ontario in both years, and along the north shore of Lake Erie in the first year. Over all sites, mean winter survival was 52%. At sites which did not completely winterkill, plant survival and yield averaged 70% and 2.38 t ha−1. Best winter rapeseed performance in Ontario occurred at well-drained sites with good winter snow cower and an absence of excessive flooding or cold temperatures in the spring. Cultivars did not differ in survival or yield in 1981–1982. The cultivar Jet Neuf had higher yields than the other cultivars in 1982–1983. There were differences in oil and protein content among the cultivars. The results suggest that winter rapeseed could become a viable crop in Ontario.Key words: Rapeseed (winter), Brassica napus, seed yield, winter survival, adaptation

scholarly journals Salicylic Acid Phytohormone Decreased the Toxicity Damage of Exogenous Lead Absorption in Seedlings of an Oilseed Plant, Brassica napus L. Grown in Hydroponic Conditions

2019 ◽  
pp. 1-15
Author(s):  
Mahdi Khozaei ◽  
Shiva Boroumand Jazi
Keyword(s):  
Salicylic Acid ◽  
Brassica Napus ◽  
Soluble Sugars ◽  
Lead Toxicity ◽  
Chlorophyll Synthesis ◽  
Dry Mass ◽  
Inhibitory Effects ◽  
Brassica Napus L ◽  
Carbohydrate Levels

Oilseed plant, Brassica napus L. seedlings grown in hydroponic condition with different concentrations of Pb were treated with salicylic acid (SA) to investigate the role of exogenous salicylic acid in alleviating lead toxicity on biochemical and physiological activities of the plant. The results showed that application of different concentrations of Pb increased soluble sugars and reduced carbohydrate levels significantly in roots and shoots of the plants. The stress induced by application of Pb triggered significant inhibitory effects on growth and chlorophyll synthesis induced on the production of protein and proline and enhanced the levels of antioxidant activity. Salicylic acid (SA) treated plants showed alleviation increasing total dry mass, leaf area, shoot and root length as well as leaf total chlorophyll content in responses to Pb stress. Results revealed the importance of salicylic acid (SA) activity in enabling plants to reduce the soluble sugars and increase of insoluble sugar in heavy -metal-stressed plants. The content of proline and proteins were also reduced in plants were treated with salicylic acid. Our data provide evidence that salicylic acid treatment decreased the activity of antioxidant enzymes in plants were exposed to different levels of Pb.

scholarly journals Polymorphism in spring and winter rapeseed varieties (Brassica napus L.) identified by ssr markers

2018 ◽  
Vol 14 (4) ◽  
pp. 366-374
Author(s):  
О. Л. Кляченко ◽  
Л. М. Присяжнюк ◽  
Н. В. Шофолова ◽  
О. В. Піскова
Keyword(s):  
Brassica Napus ◽  
Ssr Markers ◽  
Brassica Napus L ◽  
Winter Rapeseed
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