scholarly journals Optimized qRT-PCR Approach for the Detection of Intra- and Extra-Cellular SARS-CoV-2 RNAs

2020 ◽  
Vol 21 (12) ◽  
pp. 4396 ◽  
Author(s):  
Tuna Toptan ◽  
Sebastian Hoehl ◽  
Sandra Westhaus ◽  
Denisa Bojkova ◽  
Annemarie Berger ◽  
...  
Keyword(s):  
Limit Of Detection ◽  
Low Cost ◽  
World Health ◽  
Quantitative Detection ◽  
Rt Pcr ◽  
M Gene ◽  
Control And Prevention ◽  
Pcr Assays ◽  
Novel Coronavirus ◽  
Health Organization

The novel coronavirus SARS-CoV-2 is the causative agent of the acute respiratory disease COVID-19, which has become a global concern due to its rapid spread. Meanwhile, increased demand for testing has led to a shortage of reagents and supplies and compromised the performance of diagnostic laboratories in many countries. Both the World Health Organization (WHO) and the Center for Disease Control and Prevention (CDC) recommend multi-step RT-PCR assays using multiple primer and probe pairs, which might complicate the interpretation of the test results, especially for borderline cases. In this study, we describe an alternative RT-PCR approach for the detection of SARS-CoV-2 RNA that can be used for the probe-based detection of clinical isolates in diagnostics as well as in research labs using a low-cost SYBR green method. For the evaluation, we used samples from patients with confirmed SARS-CoV-2 infections and performed RT-PCR assays along with successive dilutions of RNA standards to determine the limit of detection. We identified an M-gene binding primer and probe pair highly suitable for the quantitative detection of SARS-CoV-2 RNA for diagnostic and research purposes.

scholarly journals Optimized qRT-PCR approach for the detection of intra- and extra-cellular SARS-CoV-2 RNAs

2020 ◽  
Author(s):  
Tuna Toptan ◽  
Sebastian Hoehl ◽  
Sandra Westhaus ◽  
Denisa Bojkova ◽  
Annemarie Berger ◽  
...  
Keyword(s):  
Limit Of Detection ◽  
Low Cost ◽  
World Health ◽  
Quantitative Detection ◽  
Rt Pcr ◽  
M Gene ◽  
Control And Prevention ◽  
Pcr Assays ◽  
Novel Coronavirus ◽  
Health Organization

AbstractThe novel coronavirus SARS-CoV-2 is the causative agent of the acute respiratory disease COVID-19 which has become a global concern due to its rapid spread. Meanwhile, increased demand in testing has led to shortage of reagents, supplies, and compromised the performance of diagnostic laboratories in many countries. Both the world health organization (WHO) and the Center for Disease Control and Prevention (CDC) recommend multi-step RT-PCR assays using multiple primer and probe pairs, which might complicate interpretation of the test results especially for borderline cases. In this study, we describe an alternative RT-PCR approach for the detection of SARS-CoV-2 RNA that can be used for the probe-based detection of clinical isolates in the diagnostics as well as in research labs using a low cost SYBR green method. For the evaluation, we used samples from patients with confirmed SARS-CoV-2 infection and performed RT-PCR assays along with successive dilutions of RNA standards to determine the limit of detection. We identified an M-gene binding primer and probe pair highly suitable for quantitative detection of SARS-CoV-2 RNA for diagnostic and research purposes.

scholarly journals Dual-Modal Assay Kit for the Qualitative and Quantitative Determination of the Total Water Hardness Using a Permanent Marker Fabricated Microfluidic Paper-Based Analytical Device

Chemosensors ◽  
2020 ◽  
Vol 8 (4) ◽  
pp. 97
Author(s):  
Oyejide Damilola Oyewunmi ◽  
Seyed Hamid Safiabadi-Tali ◽  
Sana Jahanshahi-Anbuhi
Keyword(s):  
Ethylenediaminetetraacetic Acid ◽  
Limit Of Detection ◽  
Low Cost ◽  
Water Hardness ◽  
Sample Introduction ◽  
World Health ◽  
Total Hardness ◽  
Quantitative Detection ◽  
Qualitative And Quantitative ◽  
Analytical Device

A dip-and-read microfluidic paper-based analytical device (µPAD) was developed for the qualitative and quantitative detection of the total hardness of water. To create well-defined hydrophobic barriers on filter paper, a regular office printer and a commercially available permanent marker pen were utilized as a quick and simple technique with easily accessible equipment/materials to fabricate µPAD in new or resource-limited laboratories without sophisticated equipment. After a wettability and barrier efficiency analysis on the permanent marker colors, the blue and green ink markers exhibited favorable hydrophobic properties and were utilized in the fabrication of the developed test devices. The device had five reaction and detection zones modeled after the classification given by the World Health Organization (WHO), so qualitatively it determined whether the water was ‘soft’, ‘moderately hard’, ‘hard’, or ‘very hard’ by changing color from blue to pink in about 3 min. The device was also used to introduce an alternative colorimetric reaction for quantitative analysis of the water hardness without the need for ethylenediaminetetraacetic acid (EDTA) and without compromising the simplicity and low cost of the device. The developed µPAD showed a calculated limit of detection (LOD) of 0.02 mM, which is at least 80% less than those of commercially available test strips and other reported µPADs, and the results of the real-world samples were consistent with those of the standard titration (with EDTA). In addition, the device exhibited stability for 2 months at room and frigid condition (4 °C) and at varying harsh temperatures from 25 to 100 °C. The results demonstrate that the developed paper-based device can be used for rapid, on-site analysis of water with no interferences and no need for a pipette for sample introduction during testing.

scholarly journals General Guidelines in the Management of an Obstetrical Patient on the Labor and Delivery Unit during the COVID-19 Pandemic

2020 ◽  
Vol 37 (08) ◽  
pp. 829-836 ◽  
Author(s):  
Angela J. Stephens ◽  
John R. Barton ◽  
Nana-Ama Ankumah Bentum ◽  
Sean C. Blackwell ◽  
Baha M. Sibai
Keyword(s):  
Health Care ◽  
Case Reports ◽  
Obstetric Care ◽  
World Health ◽  
Labor And Delivery ◽  
Health Crisis ◽  
Obstetrical Patient ◽  
Control And Prevention ◽  
Novel Coronavirus ◽  
Health Organization

Novel coronavirus disease 2019 (COVID-19) is a respiratory tract infection that was first identified in China. Since its emergence in December 2019, the virus has rapidly spread, transcending geographic barriers. The World Health Organization and the Centers for Disease Control and Prevention have declared COVID-19 as a public health crisis. Data regarding COVID-19 in pregnancy is limited, consisting of case reports and small cohort studies. However, obstetric patients are not immune from the current COVID-19 pandemic, and obstetric care will inevitably be impacted by the current epidemic. As such, clinical protocols and practice on labor and delivery units must adapt to optimize the safety of patients and health care workers and to better conserve health care resources. In this commentary, we provide suggestions to meet these goals without impacting maternal or neonatal outcomes. Key Points • Novel coronavirus disease 2019 (COVID-19) is a pandemic.• COVID-19 impacts care of obstetric patients.• Health care should be adapted for the COVID-19 pandemic.

scholarly journals Disposable silicon-based all-in-one micro-qPCR for rapid on-site detection of pathogens

2020 ◽  
Vol 11 (1) ◽  
Author(s):  
Estefania Nunez-Bajo ◽  
Alexander Silva Pinto Collins ◽  
Michael Kasimatis ◽  
Yasin Cotur ◽  
Tarek Asfour ◽  
...  
Keyword(s):  
Real Time ◽  
Limit Of Detection ◽  
Low Cost ◽  
High Specificity ◽  
Rapid Screening ◽  
Quantitative Detection ◽  
The Novel ◽  
Novel Coronavirus ◽  
Silicon Based ◽  
Specific Sequences

AbstractRapid screening and low-cost diagnosis play a crucial role in choosing the correct course of intervention when dealing with highly infectious pathogens. This is especially important if the disease-causing agent has no effective treatment, such as the novel coronavirus SARS-CoV-2, and shows no or similar symptoms to other common infections. Here, we report a disposable silicon-based integrated Point-of-Need transducer (TriSilix) for real-time quantitative detection of pathogen-specific sequences of nucleic acids. TriSilix can be produced at wafer-scale in a standard laboratory (37 chips of 10 × 10 × 0.65 mm in size can be produced in 7 h, costing ~0.35 USD per device). We are able to quantitatively detect a 563 bp fragment of genomic DNA of Mycobacterium avium subspecies paratuberculosis through real-time PCR with a limit-of-detection of 20 fg, equivalent to a single bacterium, at the 35th cycle. Using TriSilix, we also detect the cDNA from SARS-CoV-2 (1 pg) with high specificity against SARS-CoV (2003).

scholarly journals Review on Diagnostic Methods for SARS-CoV-2

Coronaviruses ◽  
2020 ◽  
Vol 01 ◽  
Author(s):  
Chandrashekhar Chauriya ◽  
Mahesh Sahu ◽  
Anuj Kumar Singh ◽  
Amita Verma
Keyword(s):  
Disease Control ◽  
Diagnostic Methods ◽  
World Health ◽  
Diagnostic Strategy ◽  
Serological Testing ◽  
Rt Pcr ◽  
Number Of Patients ◽  
Control And Prevention ◽  
Antigen Tests ◽  
Novel Coronavirus

Background: In December 2019, a large number of patients with a novel coronavirus were identified in Wuhan, China. The novel coronavirus (COVID-19) is highly contagious and increasing the rate of mortality day by day. The World Health Organization declared COVID-19 as a worldwide pandemic on March 11, 2020. Early diagnosis, of SARS-CoV-2, can restrict the COVID-19 pandemic. Objective: We aim to study the currently available diagnostic methods for COVID-19. Methods: World Health Organisation portal, Centre for Disease control and prevention portal, Indian Council of Medical Research portal, Chinese Centre for Disease Control and prevention portal, Science Direct, Google Scholar, Research Gate, etc. were searched for obtaining data. Results: Rapid diagnosis and prompt treatment can reduce the number of prospective cases. The diagnostic strategy encompasses the screening of virus with nucleic acid amplification test (NAAT) such as real-time reverse-transcription polymerase chain reaction (RT-PCR) assays. Serological testing is a diagnostic procedure used for identifying the presence of an immune responses. Radiological findings in individuals with COVID-19 were characterised by multiple areas of consolidation in chest. Rapid antigen tests are in- vitro diagnostics have been designed to give results within 10–20 min. Conclusion: Rapid, simple, and safe diagnosis of COVID-19 has a great impact on deciding clinical and epidemiological factors. RT-PCR results often require 5 to 6 hours. Diagnosis of by serological testing is not suitable but they are important epidemiologically. At present, the best radiological strategy remains undefined. Rapid antigen tests have limitations on sensitivity.

scholarly journals Multisystem inflammatory syndrome in children - a clinical example in pediatrics

2021 ◽  
Vol 25 (2) ◽  
pp. 258-260
Author(s):  
V. M. Dudnyk ◽  
V. Н. Furman ◽  
I. I. Andrikevych ◽  
N. O. Buglova ◽  
O. V. Кutsak ◽  
...  
Keyword(s):  
Serological Test ◽  
World Health ◽  
Rt Pcr ◽  
The Us ◽  
Control And Prevention ◽  
Coronavirus Infection ◽  
Polymerase Chain ◽  
Health Organization ◽  
Inflammatory Syndrome

Annotation. Peculiarities of clinical course and differential diagnosis of multisystem inflammatory syndrome (MIS-C) in children with coronavirus infection are described. The main features of this disease are long-term fever, multiorgan dysfunction, laboratory signs of inflammation and positive tests for SARS-CoV-2 (polymerase chain reaction using reverse transcription (RT-PCR), antigen test or positive serological test). The criteria of the World Health Organization (WHO) and the US Centers for Disease Control and Prevention (CDC) are used to confirm the MIS-C diagnosis.

scholarly journals An atypical case: RT-PCR-negative, sero-positive covid-19 patient

Pneumologia ◽  
2020 ◽  
Vol 69 (2) ◽  
pp. 107-114
Author(s):  
William Suriady ◽  
Andika Chandra Putra ◽  
Wiwien Heru Wiyono ◽  
Mohammad Fahmi Alatas ◽  
Bettia Bermawi ◽  
...  
Keyword(s):  
False Negative ◽  
Severe Pneumonia ◽  
World Health ◽  
Diagnostic Process ◽  
The Novel ◽  
Rt Pcr ◽  
Atypical Case ◽  
Polymerase Chain ◽  
Novel Coronavirus ◽  
Health Organization

Abstract The novel coronavirus disease-2019 (COVID-19), caused by the severe acute respiratory syndrome-coronavirus-2 (SARS-CoV-2), has become a public health emergency of international concern. The first confirmed COVID-19 case in Indonesia was announced on 2 March 2020, and later on, 11,192 confirmed cases were reported as of 3 May. The World Health Organization has stated that performing a real-time reverse transcription–polymerase chain reaction (RT-PCR) specific for SARS-CoV-2 on specimens from the upper and the lower respiratory tracts, especially nasopharyngeal and oropharyngeal swabs, is the standard diagnostic procedure for COVID-19. In Indonesia, we also use other diagnostic tests, such as rapid antibody tests specific for SARS-CoV-2. Herein, we report an atypical case of COVID-19 and describe the diagnostic process, the clinical course, with progression to severe pneumonia on Week 3 of illness and the case management. We also try to highlight the possibility of false-negative RT-PCR tests.

scholarly journals Combating the Coronavirus Pandemic: Early Detection, Medical Treatment, and a Concerted Effort by the Global Community

Research ◽  
2020 ◽  
Vol 2020 ◽  
pp. 1-35 ◽  
Author(s):  
Zichao Luo ◽  
Melgious Jin Yan Ang ◽  
Siew Yin Chan ◽  
Zhigao Yi ◽  
Yi Yiing Goh ◽  
...  
Keyword(s):  
Respiratory Illness ◽  
Viral Disease ◽  
Disease Diagnosis ◽  
World Health ◽  
Rt Pcr ◽  
Sudden Loss ◽  
Difficulty Breathing ◽  
Polymerase Chain ◽  
Novel Coronavirus ◽  
Health Organization

The World Health Organization (WHO) has declared the outbreak of 2019 novel coronavirus, known as 2019-nCoV, a pandemic, as the coronavirus has now infected over 2.6 million people globally and caused more than 185,000 fatalities as of April 23, 2020. Coronavirus disease 2019 (COVID-19) causes a respiratory illness with symptoms such as dry cough, fever, sudden loss of smell, and, in more severe cases, difficulty breathing. To date, there is no specific vaccine or treatment proven effective against this viral disease. Early and accurate diagnosis of COVID-19 is thus critical to curbing its spread and improving health outcomes. Reverse transcription-polymerase chain reaction (RT-PCR) is commonly used to detect the presence of COVID-19. Other techniques, such as recombinase polymerase amplification (RPA), loop-mediated isothermal amplification (LAMP), clustered regularly interspaced short palindromic repeats (CRISPR), and microfluidics, have allowed better disease diagnosis. Here, as part of the effort to expand screening capacity, we review advances and challenges in the rapid detection of COVID-19 by targeting nucleic acids, antigens, or antibodies. We also summarize potential treatments and vaccines against COVID-19 and discuss ongoing clinical trials of interventions to reduce viral progression.

COVID-19: Socio-Economic Consequences on the Hospitality Industry in Malaysia

2021 ◽  
Vol 12 (1) ◽  
pp. 52-52
Author(s):  
Nur Hidayah Che Ahmat ◽  
Syafiqah Rahamat ◽  
Susan Wohlsdorf Arendt
Keyword(s):  
Public Health ◽  
Hospitality Industry ◽  
Economic Consequences ◽  
World Health ◽  
The Novel ◽  
Virus Spread ◽  
The World ◽  
Control And Prevention ◽  
Novel Coronavirus ◽  
Health Organization

The novel Coronavirus disease (COVID-19) first appeared in December 2019 in Wuhan, Hubei Province China before emerging in neighbouring countries in early 2020. The World Health Organization (WHO) declared COVID-19 a pandemic when the spreading of the virus started accelerating in many parts of the world and killing thousands of people. As of 22nd May 2021, there were more than 166 million confirmed cases with more than 147 million recovered and nearly 3.5 million deaths (Worldometers, n.d.). According to the WHO (2020) and Centers for Disease Control and Prevention (2020), the virus easily spreads through coughing and sneezing. Therefore, many countries implemented social distancing between individuals and various other restriction orders or recommendations (e.g., stay-at-home policies, closure of non-essential businesses) to help curb virus spread. How governments in each country reacted to control the spread of the virus appeared crucial to mitigate public health and economic impacts. Keywords: Foodservice, Hospitality, Hotel, Malaysia, Pandemic

scholarly journals Barrier for Particle Dispersion Control During Mastoidectomy

2021 ◽  
Author(s):  
Camila Rêgo Muniz ◽  
Carlos Felype Oliveira Pena ◽  
Marcio Ribeiro Studart da Fonseca ◽  
Marcos Rabelo de Freitas
Keyword(s):  
Low Cost ◽  
Particle Dispersion ◽  
World Health ◽  
The Novel ◽  
Surgical Team ◽  
Global Pandemic ◽  
Surgical Field ◽  
Novel Coronavirus ◽  
Health Organization ◽  
Barrier Device

Abstract Introduction The China Health Authority alerted the World Health Organization (WHO) of several cases of pneumonia, and the WHO has declared the novel coronavirus (COVID-19) a global pandemic. Mastoidectomy is a high-risk aerosol generating procedure with the potential to expose the surgeon to infectious particles. Objective Aim to develop a low-cost prototype for a barrier device that can be used during mastoidectomy. Methods Describe the steps involved during otological emergency, requiring immediate surgical procedure, in untested patients. The Otorhinolaryngology Surgical Team of Walter Cantídio Hospital developed the barrier for particle dispersion presented here. Results During surgery, the prototype did not compromise visualization of the surgical field and instrumentation. Microscope repositioning was not compromised or limited by tent Instrumentation and instrument pouch under the Microscope-Tent (MT) performed surgery. After surgery, the plastic sheet was removed simply, without requiring strength. Bone dust and irrigation droplets were collected on the tent. Conclusion Our team developed and practiced, in an otologic emergency, a low-cost and reproducible barrier device that can be used in mastoidectomy in COVID-19 patients. Further tests on efficacy may be necessary.

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