Bringing New Methods to the Seed Proteomics Platform: Challenges and Perspectives

For centuries, crop plants have represented the basis of the daily human diet. Among them, cereals and legumes, accumulating oils, proteins, and carbohydrates in their seeds, distinctly dominate modern agriculture, thus play an essential role in food industry and fuel production. Therefore, seeds of crop plants are intensively studied by food chemists, biologists, biochemists, and nutritional physiologists. Accordingly, seed development and germination as well as age- and stress-related alterations in seed vigor, longevity, nutritional value, and safety can be addressed by a broad panel of analytical, biochemical, and physiological methods. Currently, functional genomics is one of the most powerful tools, giving direct access to characteristic metabolic changes accompanying plant development, senescence, and response to biotic or abiotic stress. Among individual post-genomic methodological platforms, proteomics represents one of the most effective ones, giving access to cellular metabolism at the level of proteins. During the recent decades, multiple methodological advances were introduced in different branches of life science, although only some of them were established in seed proteomics so far. Therefore, here we discuss main methodological approaches already employed in seed proteomics, as well as those still waiting for implementation in this field of plant research, with a special emphasis on sample preparation, data acquisition, processing, and post-processing. Thereby, the overall goal of this review is to bring new methodologies emerging in different areas of proteomics research (clinical, food, ecological, microbial, and plant proteomics) to the broad society of seed biologists.

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Discovering Seed Proteome: Challenges and Perspectives

10.20944/preprints201812.0129.v1 ◽

2018 ◽

Author(s):

Galina Smolikova ◽

Elena Lukasheva ◽

Gregory Mavropolo-Stolyarenko ◽

Tatiana Bilova ◽

Alena Soboleva ◽

...

Keyword(s):

Environmental Stress ◽

Seed Development ◽

Food Industry ◽

Nutritional Value ◽

Seed Vigor ◽

Crop Plants ◽

Direct Access ◽

Fuel Production ◽

Human Diet ◽

Methodological Approaches

For centuries, crop plants have represented the basis of the daily human diet. Among them, cereals and legumes, accumulating oils, proteins and carbohydrates in their seeds, distinctly dominate modern agronomic practice. Indeed, these plants play an essential role in the food industry and fuel production. Therefore, the seeds of crop plants are intensively studied by food chemists, biologists, biochemists, and nutritional physiologists. Accordingly, not only seed development and germination, but also age- and stress-related alterations in seed vigor, longevity, nutritional value and safety can be addressed by a broad panel of analytical, biochemical and physiological methods. Currently, functional genomics is one of the most powerful tools, giving direct access to characteristic metabolic changes, accompanying plant development, senescence and response to biotic or environmental stress. Among individual methodological platforms, proteomics represents one of the most effective ones, giving access to cellular metabolism at the level of proteins. Here we discuss the main methodological approaches employed by seed proteomics in the context of physiological changes related to seed development, ageing and response to environmental stress.

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Modeling Regulation of Economic Sustainability in Energy Systems with Diversified Resources

Sci ◽

10.3390/sci2010011 ◽

2020 ◽

Vol 2 (1) ◽

pp. 11

Author(s):

Anatoly Alabugin ◽

Sergei Aliukov

Keyword(s):

Jump Process ◽

Generalized Functions ◽

Multidimensional Space ◽

Economic Sustainability ◽

New Methods ◽

Control Functions ◽

New Concepts ◽

Methodological Approaches ◽

Regulatory Models

The imperfection of theoretical and methodological approaches to regulate the jump process transition when combining differentiated energy resources is a pressing issue. The goal of this paper is to develop a theory and a method to regulate the integration-balancing processes of combining diversified resources. The concept of combining integration and balancing models has been substantiated by methods of transforming multidimensional space and approximating generalized functions that represent jump-like processes. Theoretical and operational-regulatory models of economic sustainability have been developed, substantiating new concepts, patterns, properties, dependencies and indicators of the dynamics of the processes of combination; the optimality conditions for the number of approximations of generalized functions, interpreting the effects of control functions of combining resources, are determined. New methods for solving problems have been developed: the organization of the energy technology complex of facilities for enhanced resource diversification and the Center for Sustainability, improving the quality of managing dynamic processes in terms of combining and diversifying resources.

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Green revolution: Metabolic engineering of crop plants

The Biochemist ◽

10.1042/bio02804021 ◽

2006 ◽

Vol 28 (4) ◽

pp. 21-24

Author(s):

Philip John

Keyword(s):

Metabolic Engineering ◽

Nutritional Value ◽

Raw Materials ◽

Green Revolution ◽

Crop Plants ◽

Food Crops

Metabolic engineering of crop plants is being driven by the need to increase the nutritional value of our food crops, and by geopolitical forces for crops as sources of raw materials and energy.

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Variation in nutritional value of Grewia tenax fruits from different regions of Rajasthan, India

Journal of Phytology ◽

10.25081/jp.2018.v10.3420 ◽

1970 ◽

pp. 12-14

Author(s):

Mala Rathore

Keyword(s):

Trace Elements ◽

Vitamin C ◽

Protein Content ◽

Rural Economy ◽

Nutritional Value ◽

Fat Content ◽

Nutritional Composition ◽

Crop Plants ◽

Grewia Tenax

In the present investigation, an attempt has been made to identify the variation in nutritional value of Grewia tenax fruits from different regions of Rajasthan, India. Fruits were collected from Jodhpur (Kailana, Bilara) and Barmer (Siwana) region and analyzed nutritionally. Maximum ash and vitamin C content was obtained in samples from Siwana (4.73% and 56.47% respectively). Maximum fat content (2.66 %) was obtained in samples from Bilara. Maximum sugar and protein content was obtained in samples from Kailana (35.51% and 8.4% respectively).. They are rich in vitamin C (39 mg/100gm) content. The fruits are also rich sources of minerals such as Ca (0.32±0.03 g/100g), K (1.26±0.31g/100 g) and Na (1.49±0.32 g/100g) than the cultivated crop plants. Trace elements such as Fe, Zn, Cu, Mn and Mg were also analyzed. These results of nutritional composition were compared with those collected from Bilara and Siwana area of Jodhpur region. Many medicinal applications of G. tenax viz. as remedy for colds and chest complaints have also been reported. Thus, the importance of G. tenax in the rural economy is highly significant.

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Mediator Variables

Oxford Research Encyclopedia of Psychology ◽

10.1093/acrefore/9780190236557.013.19 ◽

2016 ◽

Cited By ~ 2

Author(s):

Matthew S. Fritz ◽

Houston F. Lester

Keyword(s):

Statistical Analysis ◽

Statistical Test ◽

Causal Chain ◽

Sensitivity Analyses ◽

Analysis Techniques ◽

New Methods ◽

Assumption Violations ◽

Weak Evidence ◽

Methodological Approaches ◽

The Impact

Mediator variables are variables that lie between the cause and effect in a causal chain. In other words, mediator variables are the mechanisms through which change in one variable causes change in a subsequent variable. The single-mediator model is deceptively simple because it has only three variables: an antecedent, a mediator, and a consequent. Determining that a variable functions as a mediator is a difficult process, however, because causation can be inferred only when many strict assumptions are met, including, but not limited to, perfectly reliable measures, correct temporal design, and no omitted confounders. Since many of these assumptions are difficult to assess and rarely met in practice, the significance of a statistical test of mediation alone usually provides only weak evidence of mediation. New methodological approaches are constantly being developed to circumvent these limitations. Specifically, new methods are being created for the following purposes: (1) to assess the impact of violating assumptions (e.g., sensitivity analyses) and (2) to make fewer assumptions and provide more flexible analysis techniques (e.g., Bayesian analysis or bootstrapping) that may be more robust to assumption violations. Despite these advances, the importance of the design of a study cannot be overstated. A statistical analysis, no matter how sophisticated, cannot redeem a study that measured the wrong variables or used an incorrect temporal design.

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New Methods for Specialized Lexicography: Brazilian Approach Examples [Neue Methoden der Fachlexikographie: Beispiele des brasilianischen Ansatzes / Nouvelles méthodes pour la lexicographie spécialisée: l’exemple de l’approche brésilienne]

Lexicographica - International Annual for Lexicography / Internationales Jahrbuch für Lexikographie ◽

10.1515/lexi-2014-0009 ◽

2014 ◽

Vol 30 (1) ◽

pp. 247-261

Author(s):

Maria José Bocorny Finatto

Keyword(s):

Ceramic Tiles ◽

New Methods ◽

Theoretical Approaches ◽

Methodological Approaches ◽

The Many ◽

Computational Resources ◽

Special Language

AbstractThis paper presents theoretical and methodological approaches to Terminology that are well known in Brazil, giving special attention to the production of dictionaries and similar reference work based on corpora. A collation of the many theoretical approaches and terminography practices is provided, inviting the reader to learn more about Special-Language Lexicography (SLL) in Brazil. In order to better illustrate Brazilian terminography production, a background on three different terminographic reference works recently developed in the Brazilian scenario using corpora is brought forth: a dictionary about ceramic tiles, a glossary of Oncomastology and an extensive dictionary of Dermatology. At the end of this paper, information about computational resources, such as ontologies and tools for representation of text-based content, is introduced, indicating new methodological approaches for new dictionaries.

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METHODOLOGICAL APPROACHES TO ASSESSING THE TERRITORIAL DIFFERENTIATION OF THE SOCIAL AND ECONOMIC SYSTEM OF REGIONS AND MUNICIPALITIES

Actual directions of scientific researches of the XXI century theory and practice ◽

10.34220/2308-8877-2021-8-4-102-112 ◽

2021 ◽

Vol 8 (4) ◽

pp. 102-112

Author(s):

Nataliya Yakovenko ◽

R. Ten

Keyword(s):

Economic System ◽

Practical Application ◽

Methodological Issues ◽

The Sustainable Development ◽

New Methods ◽

The Social ◽

Territorial Differentiation ◽

Territorial Disparities ◽

Methodological Approaches ◽

Methodological Assessment

One of the main internal challenges that hinder the sustainable development of Russia's regions and the transition of its economy to a new technological order is territorial social and economic differentiation. The article analyzes methodological approaches to assessing the territorial differentiation of the social and economic system of regions and municipalities. The main approaches presented in the social and economic literature have been revealed. The assessment methods used by domestic and foreign scientists are presented and critically analyzed. It is revealed that among the authors of scientific works there is still no consensus not only on the theoretical aspects, but also on the methodological issues of the procedure for assessing territorial differentiation. The systematization of the approaches and tools used by scientists from different countries has been carried out. The advantages, disadvantages and limitations for the practical application of the selected methodological approaches have been indicated. It is shown that each of the presented approaches is a basic principle when conducting territorial differentiation. Depending on the tasks set, the choice of methodological assessment approaches may vary. Clarification of existing and development of new methods for assessing sustainable socio-economic development of municipalities is the first step in creating targeted programmes to equalize territorial disparities of development in Russia.

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Voluntary national SDG reviews as a new reporting institution in a globalized society

SHS Web of Conferences ◽

10.1051/shsconf/202112910009 ◽

2021 ◽

Vol 129 ◽

pp. 10009

Author(s):

V. Novikov Andrew ◽

Sh. Urazgaliev Vladimir ◽

A. Menshikova Galina ◽

N. Soshnev Alexander

Keyword(s):

New Technology ◽

General Scheme ◽

Value Added ◽

The Third ◽

Institutional Approach ◽

Management Technology ◽

New Type ◽

Important Direction ◽

New Methodologies ◽

Methodological Approaches

Research background: Having recognized the SDGs as the most important direction of globalization processes’ implementation, we point out that assessing the results of world progress in this direction is important both in terms of assessing its dynamics and as a new technology of global governance. Assessment (both as an institution and as a management technology) is a new type of analytical and coordination interactions between countries, which is just beginning to emerge. Purpose of the article: The publication has three goals. The first is to present Voluntary national SDG reviews (VNRs) as an emerging institution for assessing and managing of global development. The second is to describe methodological approaches of their assessment, presenting them as a continuous improvement of methodology and methods that reflect new requirements for the VNRs analyses. The third is to assess the VNR of the RF (Russian Federation) - 2020, taking into account official and unofficial judgments, views from within and external experts. Methods: The publication as a whole follows an institutional approach. It provides a comparative generalization of methodological approaches to the VNRs assessment, presents expert judgments that allow to outline the compliance of VNR RF-2020 with the new valuation principles Findings & Value added:: The publication will present a general scheme of VNR institutional understanding, a comparative analysis of traditional and new methodologies for their assessment and will propose systematic (comprehensive) assessment of VNR RF-2020.

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