scholarly journals IPA1 Negatively Regulates Early Rice Seedling Development by Interfering with Starch Metabolism via the GA and WRKY Pathways

2021 ◽  
Vol 22 (12) ◽  
pp. 6605
Author(s):  
Yonggang He ◽  
Menghao Zhu ◽  
Zhihui Li ◽  
Shan Jiang ◽  
Zijun He ◽  
...  
Keyword(s):  
Seed Germination ◽  
Seedling Growth ◽  
Stress Responses ◽  
Sugar Content ◽  
Soluble Sugar ◽  
Seedling Development ◽  
Starch Metabolism ◽  
Early Seedling Growth ◽  
Amylase Genes ◽  
Early Seedling

Ideal Plant Architecture 1 (IPA1) encodes SQUAMOSA PROMOTER BINDING PROTEIN-LIKE 14 (SPL14) with a pleiotropic effect on regulating rice development and biotic stress responses. To investigate the role of IPA1 in early seedling development, we developed a pair of IPA1/ipal-NILs and found that seed germination and early seedling growth were retarded in the ipa1-NIL. Analysis of the soluble sugar content, activity of amylase, and expression of the α-amylase genes revealed that the starch metabolism was weakened in the ipa1-NIL germinating seeds. Additionally, the content of bioactive gibberellin (GA) was significantly lower than that in the IPA1-NIL seeds at 48 h of imbibition. Meanwhile, the expression of GA synthesis-related gene OsGA20ox1 was downregulated, whereas the expression of GA inactivation-related genes was upregulated in ipa1-NIL seeds. In addition, the expression of OsWRKY51 and OsWRKY71 was significantly upregulated in ipa1-NIL seeds. Using transient dual-luciferase and yeast one-hybrid assays, IPA1 was found to directly activate the expression of OsWRKY51 and OsWRKY71, which would interfere with the binding affinity of GA-induced transcription factor OsGAMYB to inhibit the expression of α-amylase genes. In summary, our results suggest that IPA1 negatively regulates seed germination and early seedling growth by interfering with starch metabolism via the GA and WRKY pathways.

scholarly journals Plant-Specific AtS40.4 Acts as a Negative Regulator in Abscisic Acid Signaling During Seed Germination and Seedling Growth in Arabidopsis

2021 ◽  
Vol 12 ◽  
Author(s):  
Xiao-Pu Shi ◽  
Jing-Jing Ren ◽  
Hao-Dong Qi ◽  
Yi Lin ◽  
Yu-Yi Wang ◽  
...  
Keyword(s):  
Abscisic Acid ◽  
Seed Germination ◽  
Seedling Growth ◽  
Stress Responses ◽  
Negative Regulator ◽  
Aba Signaling ◽  
Early Seedling Growth ◽  
Early Seedling ◽  
Increased Sensitivity ◽  
Aba Response

Abscisic acid (ABA) is an important phytohormone regulating plant growth, development and stress responses. A multitude of key factors implicated in ABA signaling have been identified; however, the regulation network of these factors needs for further information. AtS40.4, a plant-specific DUF584 domain-containing protein, was identified previously as a senescence regulator in Arabidopsis. In this study, our finding showed that AtS40.4 was negatively involved in ABA signaling during seed germination and early seedling growth. AtS40.4 was highly expressed in seeds and seedlings, and the expression level was promoted by ABA. AtS40.4 was localized both in the nucleus and the cytoplasm. Moreover, the subcellular localization pattern of AtS40.4 was affected by ABA. The knockdown mutants of AtS40.4 exhibited an increased sensitivity to ABA, whereas the overexpression of AtS40.4 decreased the ABA response during seed germination and seedling growth of Arabidopsis. Furthermore, AtS40.4 was involved in ABRE-dependent ABA signaling and influenced the expression levels of ABA INSENTIVE (ABI)1-5 and SnRK2.6. Further genetic evidence demonstrated that AtS40.4 functioned upstream of ABI4. These findings support the notion that AtS40.4 is a novel negative regulator of the ABA response network during seed germination and early seedling growth.

scholarly journals The CBL-Interacting Protein Kinase NtCIPK23 Positively Regulates Seed Germination and Early Seedling Development in Tobacco (Nicotiana tabacum L.)

Plants ◽  
2021 ◽  
Vol 10 (2) ◽  
pp. 323
Author(s):  
Sujuan Shi ◽  
Lulu An ◽  
Jingjing Mao ◽  
Oluwaseun Olayemi Aluko ◽  
Zia Ullah ◽  
...  
Keyword(s):  
Protein Kinase ◽  
Seed Germination ◽  
Seedling Growth ◽  
Hypocotyl Elongation ◽  
Interacting Protein ◽  
Knock Out ◽  
Early Seedling Growth ◽  
Tobacco Seedlings ◽  
Early Seedling ◽  
Cotyledon Expansion

CBL-interacting protein kinase (CIPK) family is a unique group of serine/threonine protein kinase family identified in plants. Among this family, AtCIPK23 and its homologs in some plants are taken as a notable group for their importance in ions transport and stress responses. However, there are limited reports on their roles in seedling growth and development, especially in Solanaceae plants. In this study, NtCIPK23, a homolog of AtCIPK23 was cloned from Nicotiana tabacum. Expression analysis showed that NtCIPK23 is mainly expressed in the radicle, hypocotyl, and cotyledons of young tobacco seedlings. The transcriptional level of NtCIPK23 changes rapidly and spatiotemporally during seed germination and early seedling growth. To study the biological function of NtCIPK23 at these stages, the overexpressing and CRISPR/Cas9-mediated knock-out (ntcipk23) tobacco lines were generated. Phenotype analysis indicated that knock-out of NtCIPK23 significantly delays seed germination and the appearance of green cotyledon of young tobacco seedling. Overexpression of NtCIPK23 promotes cotyledon expansion and hypocotyl elongation of young tobacco seedlings. The expression of NtCIPK23 in hypocotyl is strongly upregulated by darkness and inhibited under light, suggesting that a regulatory mechanism of light might underlie. Consistently, a more obvious difference in hypocotyl length among different tobacco materials was observed in the dark, compared to that under the light, indicating that the upregulation of NtCIPK23 contributes greatly to the hypocotyl elongation. Taken together, NtCIPK23 not only enhances tobacco seed germination, but also accelerate early seedling growth by promoting cotyledon greening rate, cotyledon expansion and hypocotyl elongation of young tobacco seedlings.

scholarly journals Assessment on the Effects of Potting Media on Seed Germination and Early Seedling Growth of Pterocarpus erinaceus Poir

2021 ◽  
Vol 25 (6) ◽  
pp. 969-975
Author(s):  
M.K. Peter ◽  
SIN Agera ◽  
J.I. Amonum
Keyword(s):  
Seed Germination ◽  
Seedling Growth ◽  
Growth Parameters ◽  
Cow Dung ◽  
Early Seedling Growth ◽  
Top Soil ◽  
Early Seedling ◽  
Potting Media ◽  
Number Of Leaves ◽  
Poultry Droppings

This study investigated the effects of potting media on seed germination and early seedling growth of Pterocarpus erinaceus Poir at the Forestry Nursery in Jos, Nigeria. Using Completely Randomized Design (CRD) with three replicates, laboratory-tested soil samples, top soil, sharp sand, sharp sand + top soil, sharp sand + top soil + cow dung and sharp sand + top soil + poultry droppings were used in various combinations to assess the growth parameters of P. erinaceus (germination percentage, emergence, plant height, number of leaves, length of leaves and stem diameter) for 12 weeks. Descriptive and inferential statistics were employed to analyze collected data. Result indicated that sharp sand + top soil + poultry droppings had the highest nitrogen concentration (2.19%), sharp sand + top soil + cow dung (2.07%), sharp sand + top soil (1.50%), top soil (0.72%) and Sharp sand (0.38%). Potting media with poultry droppings recorded an overall higher percentage germination of 42.9% by the end of the germination period. Analysis of variance (ANOVA) of collected data on combined soil aggregate on growth parameters indicated a significant (p<0.05) difference in plant height, number of leaves, leaf length and stem diameter. Potting with poultry dropping gave the best potting media growth results when compared to other treatments that enhanced seed germination and seedling growth of P. erinaceus. This superior observation of the poultry droppings incorporated potting mixtures over the cow dung provides an outstanding potentials to enhance P. erinaceus plantation establishment. Consequently, recommended for raising seedlings in the nursery as well as ensuring sustainable management.

scholarly journals Imbibition, Germination, and Early Seedling Growth Responses of Light Purple and Yellow Seeds of Red Clover to Distilled Water, Sodium Chloride, and Nutrient Solution

Sci ◽  
2019 ◽  
Vol 1 (2) ◽  
pp. 51 ◽  
Author(s):  
Ana Costa ◽  
Luís Dias ◽  
Alexandra Dias
Keyword(s):  
Sodium Chloride ◽  
Seed Germination ◽  
Nutrient Solution ◽  
Seedling Growth ◽  
Red Clover ◽  
Superior Performance ◽  
Distilled Water ◽  
Seed Imbibition ◽  
Early Seedling Growth ◽  
Early Seedling

The seeds of red clover are heteromorphic and two color morphs can be visually recognized, light purple and yellow, resulting from heterozygosity and recessive homozygosity at two loci. Here, we report the responses of seed imbibition, seed germination, and early seedling growth of the two morphs to distilled water, sodium chloride, and complete nutrient solution. The sensitivityof red clover seeds to treatments increased with the stage of development in what seems to be a cumulative process. No differences were found in seed imbibition between morphs or between treatments. In seedling growth, on the contrary, treatments were always effective, but differences between morphs were only observed in seeds that were treated with nutrient solution, whereas in the intermediate stage of seed germination, the effects by treatments were observed together with the appearance of differences between morphs in distilled water and in the treatment by sodium chloride solution. Simultaneously, the superior performance of the yellow morph that was found in germination, which appears to be a trait stable across cultivars of red clover seeds, turned into a superior performance of the light purple morph in seedling growth.

scholarly journals Transcriptome Analysis and Identification of Genes Associated with Starch Metabolism in Castanea henryi Seed (Fagaceae)

2020 ◽  
Vol 21 (4) ◽  
pp. 1431 ◽  
Author(s):  
Bin Liu ◽  
Ruqiang Lin ◽  
Yuting Jiang ◽  
Shuzhen Jiang ◽  
Yuanfang Xiong ◽  
...  
Keyword(s):  
Seed Germination ◽  
Candidate Genes ◽  
Starch Content ◽  
Sugar Content ◽  
Soluble Sugar ◽  
Starch Accumulation ◽  
Transcriptional Level ◽  
Potential Candidate ◽  
Starch Metabolism ◽  
Carbohydrate Storage

Starch is the most important form of carbohydrate storage and is the major energy reserve in some seeds, especially Castanea henryi. Seed germination is the beginning of the plant’s life cycle, and starch metabolism is important for seed germination. As a complex metabolic pathway, the regulation of starch metabolism in C. henryi is still poorly understood. To explore the mechanism of starch metabolism during the germination of C. henryi, we conducted a comparative gene expression analysis at the transcriptional level using RNA-seq across four different germination stages, and analyzed the changes in the starch and soluble sugar contents. The results showed that the starch content increased in 0–10 days and decreased in 10–35 days, while the soluble sugar content continuously decreased in 0–30 days and increased in 30–35 days. We identified 49 candidate genes that may be associated with starch and sucrose metabolism. Three ADP-glucose pyrophosphorylase (AGPase) genes, two nucleotide pyrophosphatase/phosphodiesterases (NPPS) genes and three starch synthases (SS) genes may be related to starch accumulation. Quantitative real-time polymerase chain reaction (qRT-PCR) was used to validate the expression levels of these genes. Our study combined transcriptome data with physiological and biochemical data, revealing potential candidate genes that affect starch metabolism during seed germination, and provides important data about starch metabolism and seed germination in seed plants.

Role of HD2 genes in seed germination and early seedling growth in Arabidopsis

2011 ◽  
Vol 30 (10) ◽  
pp. 1969-1979 ◽  
Author(s):  
Adam Colville ◽  
Reem Alhattab ◽  
Ming Hu ◽  
Hélène Labbé ◽  
Tim Xing ◽  
...  
Keyword(s):  
Seed Germination ◽  
Seedling Growth ◽  
Early Seedling Growth ◽  
Early Seedling
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