scholarly journals Biological Control of Leaf Blight Disease Caused by Pestalotiopsis maculans and Growth Promotion of Quercus acutissima Carruth Container Seedlings Using Bacillus velezensis CE 100

2021 ◽  
Vol 22 (20) ◽  
pp. 11296
Author(s):  
Sang-Jae Won ◽  
Jae-Hyun Moon ◽  
Henry B. Ajuna ◽  
Su-In Choi ◽  
Chaw Ei Htwe Maung ◽  
...  
Keyword(s):  
Cell Wall ◽  
Growth Promotion ◽  
Leaf Blight ◽  
Chemical Fertilizer ◽  
Plant Growth Promoting Bacteria ◽  
Bacillus Velezensis ◽  
Quercus Acutissima ◽  
Container Seedlings ◽  
Blight Disease ◽  
And Control

Leaf blight disease caused by Pestalotiopsismaculans lead to deleterious losses in the quality of forest container seedlings. The use of plant growth-promoting bacteria provides a promising strategy to simultaneously control diseases and enhance forest seedling production. This study investigated the biocontrol of leaf blight disease and growth promotion potential of Bacillus velezensis CE 100 in Quercus acutissima Carruth seedlings. B. velezensis CE 100 produced cell wall degrading enzymes, such as chitinase, β-l,3-glucanase, and protease, which caused cell wall lysis and hyphae deformation of P. maculans, leading to mycelial growth inhibition by 54.94%. Inoculation of B. velezensis CE 100 suppressed P. maculans infection and increased seedling survival rate by 1.6-fold and 1.3-fold compared to chemical fertilizer and control, respectively. In addition, B. velezensis CE 100 produced indole-3-acetic acid, which improved root development and nutrient uptake compared to chemical fertilizer and control. Especially, inoculation with B. velezensis CE 100 increased the total nitrogen content of Q. acutissima seedlings, improved the chlorophyll index in the leaves, and increased seedling biomass by 1.3-fold and 2.2-fold compared to chemical fertilizer and control, respectively. Thus, B. velezensis CE 100 could be applied in the eco-friendly production of high-quality forest seedlings.

scholarly journals Antifungal Activity of Bacillus velezensis CE 100 against Anthracnose Disease (Colletotrichum gloeosporioides) and Growth Promotion of Walnut (Juglans regia L.) Trees

2021 ◽  
Vol 22 (19) ◽  
pp. 10438
Author(s):  
Vantha Choub ◽  
Henry B. Ajuna ◽  
Sang-Jae Won ◽  
Jae-Hyun Moon ◽  
Su-In Choi ◽  
...  
Keyword(s):  
Colletotrichum Gloeosporioides ◽  
Growth Promotion ◽  
Juglans Regia ◽  
Total Biomass ◽  
Plant Growth Promoting Bacteria ◽  
Bacillus Velezensis ◽  
Control Groups ◽  
Field Inoculation ◽  
Anthracnose Disease ◽  
And Control

Walnut anthracnose caused by Colletotrichum gloeosporioides is a deleterious disease that severely affects the production of walnut (Juglans regia L.). The aim of this study was to assess the antifungal and growth promotion activities of Bacillus velezensis CE 100 as an alternative to chemical use in walnut production. The crude enzyme from B. velezensis CE 100 exhibited chitinase, protease, and β-l,3-glucanase activity and degraded the cell wall of C. gloeosporioides, causing the inhibition of spore germination and mycelial growth by 99.3% and 33.6% at 100 µL/mL, respectively. The field application of B. velezensis CE 100 culture broth resulted in a 1.3-fold and 6.9-fold decrease in anthracnose disease severity compared to the conventional and control groups, respectively. Moreover, B. velezensis CE 100 produced indole-3-acetic acid (up to 1.4 µg/mL) and exhibited the potential for ammonium production and phosphate solubilization to enhance the availability of essential nutrients. Thus, field inoculation of B. velezensis CE 100 improved walnut root development, increased nutrient uptake, enhanced chlorophyll content, and consequently improved total biomass by 1.5-fold and 2.0-fold compared to the conventional and control groups, respectively. These results demonstrate that B. velezensis CE 100 is an effective biocontrol agent against anthracnose disease and a potential plant growth-promoting bacteria in walnut tree production.

scholarly journals Growth promotion of transplanted rice plant by bioagents effective against bacterial leaf blight disease of rice under glasshouse conditions

2014 ◽  
Vol 6 (1) ◽  
pp. 234-238
Author(s):  
Gokil Prasad Gangwar ◽  
A. P. Sinha
Keyword(s):  
Grain Yield ◽  
Growth Promotion ◽  
Foliar Spray ◽  
Leaf Blight ◽  
Bacterial Leaf Blight ◽  
Total Phenolic ◽  
Root Weight ◽  
Transplanted Rice ◽  
Untreated Check ◽  
Blight Disease

The experiment was conducted to evaluate growth promotion of transplanted rice by fungal and bacterial bioagents effective against bacterial leaf blight of rice, under glasshouse conditions. Bioagent formulations (PBA -1, PBA -2, FLP 88, Pf 83, Isolate 40 and T. harzianum) were applied as seed treatment, seedling root dip and foliar spray and compared with chemical treatment and untreated check. All bioagent formulations were significantly effective in increasing number of tillers per hill, root length, total phenolic content in rice leaves, fresh and dry root weight, fresh and dry shoot weight, flag leaf area, grain yield and 1000 grain weight over check. Present study revealed that potential fungal and bacterial bioagents effective against bacterial leaf blight disease of rice, exhibited plant growth promoting activities and resulted 22.38 to 32.08 % increase in grain yield over untreated check, even if there is no disease.

scholarly journals Isolation and Characterization of Salt Tolerant Endophytic and Rhizospheric Plant Growth-Promoting Bacteria (PGPB) Associated with the Halophyte Plant (Sesuvium Verrucosum) Grown in KSA

2015 ◽  
Vol 3 (3) ◽  
pp. 552-560 ◽  
Author(s):  
Mohamed A.M. El-Awady ◽  
Mohamed M. Hassan ◽  
Yassin M. Al-Sodany
Keyword(s):  
Nitrogen Fixation ◽  
Acetic Acid ◽  
Plant Growth ◽  
Growth Promotion ◽  
Exponential Growth Phase ◽  
Plant Growth Promoting Bacteria ◽  
Salt Tolerant ◽  
Isolation And Characterization ◽  
Growth Promoting

This study was designed to isolate and characterize endophytic and rhizospheric bacteria associated with the halophyte plant Sesuvium verrucosum, grown under extreme salinity soil in Jeddah, Saudi Arabia. The plant growth promotion activities of isolated bacterial were evaluated in vitro. A total of 19 salt tolerant endophytic and rhizospheric bacterial isolates were obtained and grouped into six according to genetic similarity based on RAPD data. These six isolates were identified by amplification and partial sequences of 16S rDNA as Enterobacter cancerogenus,Vibrio cholerae, Bacillus subtilis, Escherichia coli and two Enterobacter sp. Isolates were then grown until exponential growth phase to evaluate the atmospheric nitrogen fixation, phosphate solubilization, and production of phytohormones such as indole-3-acetic acid, as well as 1-aminocyclopropane-1-carboxylate (ACC) deaminase activity. While, All of the six strains were negative for ACC deaminaseactivity, two isolates showed Nitrogen fixation activity, three isolates produce the plant hormone (Indole acetic acid) and two isolates have the activity of solubiliztion of organic phosphate. Among the six isolates, the isolate (R3) from the soil around the roots is able to perform the three previous growth promoting possibilities together and it is ideal for use in promoting the growth of plants under the high salinity conditions. This isolate is candidate to prepare a friendly biofertelizer that can be used for the improvement of the crops performance under salinity conditions.Int J Appl Sci Biotechnol, Vol 3(3): 552-560

scholarly journals Combined Application of Rhizosphere Bacteria with Endophytic Bacteria Suppresses Root Diseases and Increases Productivity of Black Pepper (Piper nigrum L.)

Agriculture ◽  
2020 ◽  
Vol 11 (1) ◽  
pp. 15
Author(s):  
Sy Dinh Nguyen ◽  
Thi Huyen Trang Trinh ◽  
Trung Dzung Tran ◽  
Tinh Van Nguyen ◽  
Hoang Van Chuyen ◽  
...  
Keyword(s):  
Bacillus Subtilis ◽  
Endophytic Bacteria ◽  
Growth Promotion ◽  
Black Pepper ◽  
Rhizosphere Bacteria ◽  
Growth And Yield ◽  
Piper Nigrum ◽  
Bacillus Velezensis ◽  
Root Knot Nematode ◽  
Central Highland

Black pepper (Piper nigrum L.) is one of the most important crops and global demand continues to increase, giving it a high export value. However, black pepper cultivation has been seriously affected by a number of pathogenic diseases. Among them, “quick wilt” caused by Phytophthora sp., “slow decline” caused by Fusarium sp., and root-knot nematode Meloidogyne sp. have a serious negative effect on black pepper growth and productivity. There have been different chemical and biological methods applied to control these diseases, but their effectiveness has been limited. The aim of this research was to evaluate different combinations of rhizosphere bacteria and endophytic bacteria isolated from black pepper farms in the Central Highland of Vietnam for their ability to suppress pathogens and promote black pepper growth and yield. Formula 6, containing the strains Bacillus velezensis KN12, Bacillus amyloliquefaciens DL1, Bacillus velezensis DS29, Bacillus subtilis BH15, Bacillus subtilis V1.21 and Bacillus cereus CS30 exhibited the largest effect against Phytophthora and Fusarium in the soil and in the roots of black pepper. These bio-products also increased chlorophyll a and b contents, which led to a 1.5-fold increase of the photosynthetic intensity than the control formula and a 4.5% increase in the peppercorn yield (3.45 vs. 3.30 tons per hectare for the control). Our results suggest that the application of rhizosphere and endophytic bacteria is a promising method for disease control and growth-promotion of black pepper.

scholarly journals The Root Microbiome of Salicornia ramosissima as a Seedbank for Plant-Growth Promoting Halotolerant Bacteria

2021 ◽  
Vol 11 (5) ◽  
pp. 2233
Author(s):  
Maria J. Ferreira ◽  
Angela Cunha ◽  
Sandro Figueiredo ◽  
Pedro Faustino ◽  
Carla Patinha ◽  
...  
Keyword(s):  
Plant Growth ◽  
Plant Growth Promotion ◽  
Growth Promotion ◽  
Cultivated Plants ◽  
Plant Growth Promoting Bacteria ◽  
Crop Cultivation ◽  
Halotolerant Bacteria ◽  
Salicornia Ramosissima ◽  
Plant Growth Promoting ◽  
Growth Promoting

Root−associated microbial communities play important roles in the process of adaptation of plant hosts to environment stressors, and in this perspective, the microbiome of halophytes represents a valuable model for understanding the contribution of microorganisms to plant tolerance to salt. Although considered as the most promising halophyte candidate to crop cultivation, Salicornia ramosissima is one of the least-studied species in terms of microbiome composition and the effect of sediment properties on the diversity of plant-growth promoting bacteria associated with the roots. In this work, we aimed at isolating and characterizing halotolerant bacteria associated with the rhizosphere and root tissues of S. ramosissima, envisaging their application in saline agriculture. Endophytic and rhizosphere bacteria were isolated from wild and crop cultivated plants, growing in different estuarine conditions. Isolates were identified based on 16S rRNA sequences and screened for plant-growth promotion traits. The subsets of isolates from different sampling sites were very different in terms of composition but consistent in terms of the plant-growth promoting traits represented. Bacillus was the most represented genus and expressed the wider range of extracellular enzymatic activities. Halotolerant strains of Salinicola, Pseudomonas, Oceanobacillus, Halomonas, Providencia, Bacillus, Psychrobacter and Brevibacterium also exhibited several plant-growth promotion traits (e.g., 3-indole acetic acid (IAA), 1-aminocyclopropane-1-carboxylic acid (ACC) deaminase, siderophores, phosphate solubilization). Considering the taxonomic diversity and the plant-growth promotion potential of the isolates, the collection represents a valuable resource that can be used to optimize the crop cultivation of Salicornia under different environmental conditions and for the attenuation of salt stress in non-halophytes, considering the global threat of arable soil salinization.

scholarly journals The Effects of Plant Growth-Promoting Bacteria with Biostimulant Features on the Growth of a Local Onion Cultivar and a Commercial Zucchini Variety

Agronomy ◽  
2021 ◽  
Vol 11 (5) ◽  
pp. 888
Author(s):  
Giorgia Novello ◽  
Patrizia Cesaro ◽  
Elisa Bona ◽  
Nadia Massa ◽  
Fabio Gosetti ◽  
...  
Keyword(s):  
Plant Growth ◽  
Growth Promotion ◽  
Global Market ◽  
Plant Growth Promoting Bacteria ◽  
Bacterial Strains ◽  
Mineral Concentration ◽  
Positive Effects ◽  
Plant Growth Promoting ◽  
Onion Cultivar ◽  
Growth Promoting

The reduction of chemical inputs due to fertilizer and pesticide applications is a target shared both by farmers and consumers in order to minimize the side effects for human and environmental health. Among the possible strategies, the use of biostimulants has become increasingly important as demonstrated by the fast growth of their global market and by the increased rate of registration of new products. In this work, we assessed the effects of five bacterial strains (Pseudomonas fluorescens Pf4, P. putida S1Pf1, P. protegens Pf7, P. migulae 8R6, and Pseudomonas sp. 5Vm1K), which were chosen according to their previously reported plant growth promotion traits and their positive effects on fruit/seed nutrient contents, on a local onion cultivar and on zucchini. The possible variations induced by the inoculation with the bacterial strains on the onion nutritional components were also evaluated. Inoculation resulted in significant growth stimulation and improvement of the mineral concentration of the onion bulb, induced particularly by 5Vm1K and S1Pf1, and in different effects on the flowering of the zucchini plants according to the bacterial strain. The present study provides new information regarding the activity of the five plant growth-promoting bacteria (PGPB) strains on onion and zucchini, two plant species rarely considered by the scientific literature despite their economic relevance.

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