scholarly journals Persistence of a Yeast-Based (Hanseniaspora uvarum) Attract-and-Kill Formulation against Drosophila suzukii on Grape Leaves

Insects ◽  
2020 ◽  
Vol 11 (11) ◽  
pp. 810
Author(s):  
Flavia Bianchi ◽  
Urban Spitaler ◽  
Irene Castellan ◽  
Carlo S. Cossu ◽  
Timothy Brigadoi ◽  
...  
Keyword(s):  
Fermentation Broth ◽  
Exchange Chromatography ◽  
Liquid Chromatography Mass Spectrometry ◽  
Drosophila Suzukii ◽  
Potato Dextrose Broth ◽  
Hanseniaspora Uvarum ◽  
Leaf Surfaces ◽  
Volatile Organic ◽  
Attract And Kill ◽  
Over Time

The production of phagostimulant and attractive volatile organic compounds (VOCs) by yeasts can be exploited to improve the efficacy of attract-and-kill formulations against the spotted wing drosophila (SWD). This study evaluated the persistence over one week of a yeast-based formulation under greenhouse conditions. Potted grape plants were treated with: (i) potato dextrose broth (PDB), (ii) PDB containing spinosad (PDB + S), and (iii) H. uvarum fermentation broth grown on PDB containing spinosad (H. u. + S). Laboratory trials were performed to determine the survival and the oviposition rate of SWD after exposure to treated leaves. Ion-exchange chromatography was performed to measure carbohydrates, sugar alcohols, and organic acids on leaf surfaces, while amino acids were assessed through liquid chromatography–mass-spectrometry. Additionally, the VOCs released by plants treated with H.uvarum were collected via closed-loop-stripping analysis and compared to those emitted by untreated leaves. A higher mortality was observed for adult SWDs in contact with H. uvarum containing spinosad compared to PDB containing spinosad. Generally, a decrease in the amounts of non-volatile compounds was observed over time, though numerous nutrients were still present one week after treatment. The application of the yeast-based formulation induced the emission of VOCs by the treated leaves. The concentration of 2-phenylethanol, one of the main VOCs emitted by yeasts, decreased over time. These findings describe the presence of potential phagostimulants and compounds attractive to SWD in a yeast-based attract-and-kill formulation and demonstrate the efficacy of the formulation over one week.

scholarly journals Separate and combined Hanseniaspora uvarum and Metschnikowia pulcherrima metabolic volatiles are attractive to Drosophila suzukii in the laboratory and field

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
R. Jones ◽  
M. T. Fountain ◽  
C. S. Günther ◽  
P. E. Eady ◽  
M. R. Goddard
Keyword(s):  
Yeast Species ◽  
Field Trapping ◽  
Field Trials ◽  
Economic Losses ◽  
Drosophila Suzukii ◽  
Control Efficacy ◽  
Hanseniaspora Uvarum ◽  
Metschnikowia Pulcherrima ◽  
Rich Diversity ◽  
Relative Attractiveness

AbstractDrosophila suzukii flies cause economic losses to fruit crops globally. Previous work shows various Drosophila species are attracted to volatile metabolites produced by individual fruit associated yeast isolates, but fruits naturally harbour a rich diversity of yeast species. Here, we report the relative attractiveness of D. suzukii to yeasts presented individually or in combinations using laboratory preference tests and field trapping data. Laboratory trials revealed four of 12 single yeast isolates were attractive to D. suzukii, of which Metschnikowia pulcherrima and Hanseniaspora uvarum were also attractive in field trials. Four out of 10 yeast combinations involving Candida zemplinina, Pichia pijperi, M. pulcherrima and H. uvarum were attractive in the laboratory. Whilst a combination of M. pulcherrima + H. uvarum trapped the greatest number of D. suzukii in the field, the efficacy of the M. pulcherrima + H. uvarum combination to trap D. suzukii was not significantly greater than traps primed with volatiles from only H. uvarum. While volatiles from isolates of M. pulcherrima and H. uvarum show promise as baits for D. suzukii, further research is needed to ascertain how and why flies are attracted to certain baits to optimise control efficacy.

scholarly journals Specific Non-Reducing Ends in Heparins from Different Animal Origins: Building Blocks Analysis Using Reductive Amination Tagging by Sulfanilic Acid

Molecules ◽  
2020 ◽  
Vol 25 (23) ◽  
pp. 5553
Author(s):  
Pierre A. J. Mourier
Keyword(s):  
Building Block ◽  
Reductive Amination ◽  
Building Blocks ◽  
Anion Exchange Chromatography ◽  
Sulfanilic Acid ◽  
Exchange Chromatography ◽  
Sulfated Polysaccharides ◽  
Liquid Chromatography Mass Spectrometry ◽  
Anticoagulant Drugs ◽  
Strong Anion Exchange

Heparins are linear sulfated polysaccharides widely used as anticoagulant drugs. Their nonreducing-end (NRE) has been little investigated due to challenges in their characterization, but is known to be partly generated by enzymatic cleavage with heparanases, resulting in N-sulfated glucosamines at the NRE. Uronic NRE (specifically glucuronic acids) have been isolated from porcine heparin, with GlcA-GlcNS,3S,6S identified as a porcine-specific NRE marker. To further characterize NRE in heparinoids, a building block analysis involving exhaustive heparinase digestion and subsequent reductive amination with sulfanilic acid was performed. This study describes a new method for identifying heparin classical building blocks and novel NRE building blocks using strong anion exchange chromatography on AS11 columns for the assay, and ion-pair liquid chromatography-mass spectrometry for building block identification. Porcine, ovine, and bovine intestine heparins were analyzed. Generally, NRE on these three heparins are highly sulfated moieties, particularly with 3-O sulfates, and the observed composition of the NRE is highly dependent on heparin origin. At the highest level of specificity, the isolated marker was only detected in porcine heparin. However, the proportion of glucosamines in the NRE and the proportion of glucuronic/iduronic configurations in the NRE uronic moieties greatly varied between heparin types.

scholarly journals Comparative Lipidomics of Different Yeast Species Associated to Drosophila suzukii

Metabolites ◽  
2020 ◽  
Vol 10 (9) ◽  
pp. 352
Author(s):  
Flavia Bianchi ◽  
Urban Spitaler ◽  
Peter Robatscher ◽  
Rudi F. Vogel ◽  
Silvia Schmidt ◽  
...  
Keyword(s):  
Lipid Profile ◽  
Yeast Species ◽  
Enrichment Analysis ◽  
Laboratory Strain ◽  
Drosophila Suzukii ◽  
Candida Sp ◽  
Chemical Enrichment ◽  
Hanseniaspora Uvarum ◽  
Behavioral Trials ◽  
The Relationship

Yeasts constitute a dietary source for the spotted wing drosophila (SWD) and produce compounds that attract these flies. The study of the chemical composition of the yeast communities associated with SWD should therefore help to understand the relationship between the biology of the insect and the yeast’s metabolism. In the present study, the lipidome of five yeast species isolated from grapes infested by SWD (three Hanseniaspora uvarum strains, Candida sp., Issatchenkia terricola, Metschnikowia pulcherrima and Saccharomycopsis vini) and a laboratory strain of Saccharomyces cerevisiae was explored using an untargeted approach. Additionally, the lipid profile of two species, S. cerevisiae and H. uvarum, which were reported to elicit different responses on SWD flies based on feeding and behavioral trials, was compared with a chemical enrichment approach. Overall, 171 lipids were annotated. The yeast species could be distinguished from each other based on their lipid profile, except for the three strains of H. uvarum, which were very similar to each other. The chemical enrichment analysis emphasized diversities between S. cerevisiae and H. uvarum, that could not be detected based on their global lipid profile. The information concerning differences between species in their lipidome may be of interest to future entomological studies concerning the yeast-insect interaction and could help to explain the responses of SWD to diverse yeast species.

scholarly journals Microbiota-mediated competition between pest insects and its application to the evolution-proof protection of crops

2020 ◽  
Author(s):  
Antoine Rombaut ◽  
Romain Gallet ◽  
Kenza Qitout ◽  
Mukherjy Samy ◽  
Robin Guilhot ◽  
...  
Keyword(s):  
Drosophila Melanogaster ◽  
Ecological Niche ◽  
Insect Pest ◽  
Adaptive Significance ◽  
Condition Dependence ◽  
Larval Competition ◽  
Drosophila Suzukii ◽  
Pest Insects ◽  
Competition Avoidance ◽  
Over Time

AbstractSpecies that share the same resources often avoid costly competition with contextdependent behaviors. This is the case of the invasive insect pest Drosophila suzukii which larvae’s ecological niche overlaps with that of Drosophila melanogaster in ripe, but not rotten, fruit. Previous research showed D. suzukii prevents costly larval competition by not ovipositing in substrates with D. melanogaster eggs. We discovered D. suzukii females respond to cues produced by D. melanogaster adults associated to gut microbiota bacteria. This behavior of D. suzukii varied over time and among populations, revealing subtle condition-dependence. In particular, D. suzukii females that bore D. melanogaster gut bacteria stopped avoiding sites with D. melanogaster cues. The adaptive significance of the behavior was investigated by reproducing experimentally in-fruit larval competition. D. suzukii larvae only suffered from competition with D. melanogaster if the competitor was associated to its microbiota, suggesting D. suzukii has evolved a solution to avoid its offspring develops in challenging environments. We argue that D. suzukii’s competition avoidance behavior has features enabling the design of an evolution-proof repellent to protect crops.

scholarly journals Turnover rate of coenzyme A in mouse brain and liver

PLoS ONE ◽  
2021 ◽  
Vol 16 (5) ◽  
pp. e0251981
Author(s):  
Laura Orsatti ◽  
Maria Vittoria Orsale ◽  
Pamela di Pasquale ◽  
Andrea Vecchi ◽  
Fabrizio Colaceci ◽  
...  
Keyword(s):  
Half Life ◽  
Turnover Rate ◽  
Coenzyme A ◽  
Pantothenic Acid ◽  
Male Mice ◽  
Liquid Chromatography Mass Spectrometry ◽  
Pantothenate Kinase ◽  
Male And Female ◽  
The Brain ◽  
Over Time

Coenzyme A (CoA) is a fundamental cofactor involved in a number of important biochemical reactions in the cell. Altered CoA metabolism results in severe conditions such as pantothenate kinase-associated neurodegeneration (PKAN) in which a reduction of the activity of pantothenate kinase isoform 2 (PANK2) present in CoA biosynthesis in the brain consequently lowers the level of CoA in this organ. In order to develop a new drug aimed at restoring the sufficient amount of CoA in the brain of PKAN patients, we looked at its turnover. We report here the results of two experiments that enabled us to measure the half-life of pantothenic acid, free CoA (CoASH) and acetylCoA in the brains and livers of male and female C57BL/6N mice, and total CoA in the brains of male mice. We administered (intrastriatally or orally) a single dose of a [13C3-15N-18O]-labelled coenzyme A precursor (fosmetpantotenate or [13C3-15N]-pantothenic acid) to the mice and measured, by liquid chromatography-mass spectrometry, unlabelled- and labelled-coenzyme A species appearance and disappearance over time. We found that the turnover of all metabolites was faster in the liver than in the brain in both genders with no evident gender difference observed. In the oral study, the CoASH half-life was: 69 ± 5 h (male) and 82 ± 6 h (female) in the liver; 136 ± 14 h (male) and 144 ± 12 h (female) in the brain. AcetylCoA half-life was 74 ± 9 h (male) and 71 ± 7 h (female) in the liver; 117 ± 13 h (male) and 158 ± 23 (female) in the brain. These results were in accordance with the corresponding values obtained after intrastriatal infusion of labelled-fosmetpantotenate (CoASH 124 ± 13 h, acetylCoA 117 ± 11 and total CoA 144 ± 17 in male brain).

scholarly journals Measuring sucrose in blood after oral administration to detect abomasal ulcers in calves

2019 ◽  
Vol 31 (5) ◽  
pp. 737-741
Author(s):  
Alexandra Hund ◽  
Armin Schaffer ◽  
Marlies Dolezal ◽  
Hermann Mascher ◽  
Thomas Wittek
Keyword(s):  
Mass Spectrometry ◽  
Oral Administration ◽  
Mixed Models ◽  
Sucrose Solution ◽  
Terminally Ill ◽  
Liquid Chromatography Mass Spectrometry ◽  
Serum Samples ◽  
Blood Samples ◽  
Highperformance Liquid Chromatography ◽  
Over Time

Abomasal ulcers are common in cattle, especially in calves, and to date, there is no reliable antemortem method for diagnosis, to our knowledge. We assessed if measuring sucrose in blood after oral administration in calves could be used to identify animals with abomasal ulcers. Terminally ill calves ( n = 12; part A) and calves designated for slaughter ( n = 123; part B) were given a sucrose solution per os, and blood samples were taken 15, 30, 60, 90, and 120 min (part A) or 30 and 60 min (part B) after administration. The calves were then euthanized or slaughtered, and their abomasa were examined. Serum samples were analyzed using highperformance liquid chromatography-mass spectrometry, and data were analyzed using general linear mixed models. Calves both with and without affected abomasa had increasing sucrose values over time without significant differences. Also, there was no relationship between the size of the mucosal lesion and sucrose values.

scholarly journals SPME collection and GC-MS analysis of volatiles emitted during the attack of male Polygraphus poligraphus (Coleoptera, Curcolionidae) on Norway spruce

2015 ◽  
Vol 70 (9-10) ◽  
pp. 265-273 ◽  
Author(s):  
Rizan Rahmani ◽  
Erik Hedenström ◽  
Martin Schroeder
Keyword(s):  
Norway Spruce ◽  
Bark Beetle ◽  
Bark Beetles ◽  
Tree Mortality ◽  
Solid Phase ◽  
Enantiomeric Excess ◽  
Enantiomeric Purity ◽  
Spruce Bark ◽  
Volatile Organic ◽  
Over Time

Abstract Tree mortality caused by bark beetles has increased in recent decades in both Europe and North America. In a large recent outbreak in central Sweden the bark beetle Polygraphus poligraphus was often found together with the spruce bark beetle Ips typographus in killed trees. To increase the understanding of the aggregation behavior of P. poligraphus we used solid phase microextraction (SPME) to collect volatile organic compounds (VOCs) released from single P. poligraphus males, with and without added females, colonizing Norway spruce stem sections and analyzed the sampled compounds by combined gas chromatography and mass spectrometry (GC-MS). High amounts of terpinen-4-ol, a substance found in the hindguts of P. poligraphus males in earlier studies, were released by colonizing males. The emission of both enantiomers of terpinen-4-ol was monitored by GC-MS over time as the males aged in the absence and presence of females. Single males emitted (R)-(–)-terpinen-4-ol for up to 60 days in high enantiomeric purity but the enantiomeric excess (ee) varied between males, and also for the same individual, over time from 96.3% to 99.3% ee. In the presence of females, males also emitted terpinen-4-ol for up to 50 days but now in lower amounts and with lower enantiomeric purity varying from 67.7% ee to 99.3% ee. Small quantities of other volatile compounds were emitted from the colonizing beetles including cis- and trans-4-thujanol, both of which were previously shown to be present in the hindguts of males. In earlier studies frontalin was found to attract P. poligraphus, but in our study it was not identified among emitted compounds from colonizing beetles.

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