Diagnosing Fungal Keratitis and Simultaneously Identifying Fusarium and Aspergillus Keratitis with a Dot Hybridization Array

Fungal keratitis (FK) is one of the most common microbial keratitis, which often leads to poor prognosis as a result of delayed diagnosis. Several studies implied that early differentiation of the two major FK, Fusarium and Aspergillus keratitis, could be helpful in selecting effective anti-fungal regimens. Therefore, a novel dot hybridization array (DHA) was developed to diagnose FK and differentiate Fusarium and Aspergillus keratitis in this study. One hundred forty-six corneal scrapes obtained from one hundred forty-six subjects impressed with clinically suspected FK were used to evaluate the performance of the DHA. Among these patients, 107 (73.3%) patients had actual FK confirmed by culture and DNA sequencing. We found that the DHA had 93.5% sensitivity and 97.4% specificity in diagnosing FK. In addition, this array had 93.2% sensitivity and 93.8% specificity in diagnosing Fusarium keratitis, as well as 83.3% sensitivity and 100% specificity in diagnosing Aspergillus keratitis. Furthermore, it had 83.9% sensitivity and 100% specificity in identifying Fusarium solani keratitis. Thus, this newly developed DHA will be beneficial to earlier diagnosis, more precise treatment, and improve prognosis of FK, by minimizing medical refractory events and surgical needs.

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Anti-fungal activities of constituents from Uvaria scheffleri and Artabotrys brachypetalus

Journal of Agricultural Sciences Belgrade ◽

10.2298/jas0601079o ◽

2006 ◽

Vol 51 (1) ◽

pp. 79-86 ◽

Cited By ~ 1

Author(s):

A.C. Odebode ◽

S.A. Jonker ◽

C.C. Joseph ◽

S.W. Wachira

Keyword(s):

Fusarium Solani ◽

Radial Growth ◽

Fungal Pathogens ◽

Dry Weight ◽

Stem Bark ◽

Inhibitory Effect ◽

In Vitro Bioassay ◽

Botryodiplodia Theobromae ◽

Anti Fungal

The anti-fungal activity of schefflone, a mixture of dimmer, 3,5 dimethoxy carvacrol and annonaceous acetogenin, extracted from stem-bark and root of Uvaria scheffleri and Artabotrys bruchypetalus against Fusarium solani, Botryodiplodia theobromae, Asperillus niger and Aspergillus flavus was determined. An in-vitro bioassay showed that the minimum inhibitory effect of the compounds to the fungal pathogens occurred at 200 ppm in both radial growth and mycelia dry weight measurements. Acetogenin from A brachypetalus had a very strong anti-fungal effect on all the test fungi. The effects of the compounds were more pronounced on F solani than on the other. The bioassay methods also play a significant role in the sensitivity of the samples on the pathogens. .

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Mucinous Adenocarcinoma Associated with Fistula in an 87-Year-Old Managed with Neoadjuvant Chemoradiation and ELAPE at Tata Memorial Hospital, in Mumbai

Global Surgery Case Reports ◽

10.31487/j.gscr.2020.02.09 ◽

2020 ◽

pp. 1-4

Author(s):

Avanish Saklani ◽

Seke Manase Ephraim KAZUMA ◽

Vivek Sukumar ◽

Avanish Saklani

Keyword(s):

Colorectal Cancer ◽

Distant Metastasis ◽

Mucinous Adenocarcinoma ◽

Poor Prognosis ◽

Locally Advanced ◽

Delayed Diagnosis ◽

Neoadjuvant Chemoradiation ◽

Tata Memorial Hospital ◽

The Third ◽

Cutaneous Flap

Colorectal cancer is the third most diagnosed cancer and the fourth leading cancer-related death worldwide. Mucinous adenocarcinoma associated with anal fistula is a rare variant of adenocarcinoma, presents with delayed diagnosis, locally advanced, low nodal, and no distant metastasis. Adenocarcinoma associated with fistula (ACAF) is rare, has delayed diagnosis and poor prognosis but can be managed with neoadjuvant chemoradiation (NACRT) and complete curative resections with reconstruction by V-Y advancement cutaneous flap.

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The Mote in thy Brother’s Eyes - Fusarium Solani in Leukemia Host

10.21203/rs.3.rs-787465/v1 ◽

2021 ◽

Author(s):

Rawan AlAgha ◽

Wee Lee Chan ◽

Thong Edwin Wei Sheng ◽

Joanne Lee ◽

Jen Wei Ying ◽

...

Keyword(s):

Case Report ◽

Early Diagnosis ◽

High Mortality ◽

Combination Chemotherapy ◽

Fusarium Solani ◽

Immunocompromised Host ◽

Skin Lesions ◽

Acute Onset ◽

Source Control ◽

Anti Fungal

Abstract Fusariosis is increasingly seen among immunocompromised host. The organism is known for its virulence and spectrum of infections. Presenting here a case of relapse acute myeloblastic leukemia on chemotherapy with acute onset of red painful eye followed by widespread painful skin lesions. Microbiological and radiological investigations diagnosed her with disseminated fusariosis. Treatment was challenging in view its inherent resistance to multiple anti-fungal agents and the need for early aggressive source control. The case report reflects the importance of early diagnosis and combination chemotherapy to salvage the patient from high mortality.

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Anti-fungal activity of bacterial endophytes associated with legumes against Fusarium solani: Assessment of fungi soil suppressiveness and plant protection induction

Applied Soil Ecology ◽

10.1016/j.apsoil.2017.10.025 ◽

2018 ◽

Vol 124 ◽

pp. 131-140 ◽

Cited By ~ 14

Author(s):

Ameni Bahroun ◽

Alexandre Jousset ◽

Rakia Mhamdi ◽

Moncef Mrabet ◽

Haythem Mhadhbi

Keyword(s):

Fusarium Solani ◽

Plant Protection ◽

Bacterial Endophytes ◽

Soil Suppressiveness ◽

Fungal Activity ◽

Anti Fungal

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Fungal Peritonitis Due to Fusarium solani Species Complex Sequential Isolates Identified with DNA Sequencing in a Kidney Transplant Recipient in Brazil

Mycopathologia ◽

10.1007/s11046-015-9929-7 ◽

2015 ◽

Vol 180 (5-6) ◽

pp. 397-401 ◽

Cited By ~ 5

Author(s):

Walicyranison Plinio da Silva-Rocha ◽

Diana Luzia Zuza-Alves ◽

Analy Salles de Azevedo Melo ◽

Guilherme Maranhão Chaves

Keyword(s):

Transplant Recipient ◽

Dna Sequencing ◽

Kidney Transplant ◽

Fusarium Solani ◽

Species Complex ◽

Kidney Transplant Recipient ◽

Fungal Peritonitis ◽

Fusarium Solani Species Complex

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Disseminated Phaeohyphomycotic Lymphadenitis with Cladophialophora Species

EMJ Microbiology & Infectious Diseases ◽

10.33590/emjmicrobiolinfectdis/20-00276 ◽

2021 ◽

Author(s):

Safia Moin ◽

Faisal Mahmood ◽

Joveria Farooqi ◽

Faheem Naqvi ◽

Romana Idress ◽

...

Keyword(s):

Delayed Diagnosis ◽

Granulomatous Inflammation ◽

Giant Cells ◽

Young Female ◽

Biopsy Material ◽

Appropriate Treatment ◽

Clinical Presentations ◽

Fungal Hyphae ◽

Conclusive Diagnosis ◽

Anti Fungal

Objective: This study describes a case of disseminated phaeohyphomycotic lymphadenitis in a young female with delayed diagnosis and good clinical response after appropriate treatment. Methods: A 32-year-old female presented with erythematous to violaceous papules with oozing discharge bilaterally in her inguinal region for a few months. History revealed tuberculous meningitis 4 years earlier treated with first line anti-tuberculous therapy for 18 months, and 2 years previously she developed pigmented discharging lymph nodes bilaterally in her axillae. The histopathology of the biopsy of the axillary nodes showed chronic granulomatous inflammation with multiple branching septate fungal hyphae. She received amphotericin B for 21 days but without improvement. Biopsy from the inguinal lesions was sent for histopathology and culture. Results: Histopathology of the biopsy material showed chronic granulomatous inflammatory process with multinucleate giant cells, epithelioid cells, histiocytes, and lymphocytes with multiple branching septate fungal hyphae. Gram stain revealed moderate septate hyphae with numerous pus cells. Culture on Sabouraud dextrose agar yielded velvety olive–black colonies in the fourth week. Microscopic slide examination of culture material was suggestive of Cladophialophora species. The patient was started on voriconazole, which was continued for 6 months, and showed clinical improvement. Conclusion: Incomplete investigation of infectious lesions may delay diagnosis. Furthermore, clinical presentations are greatly influenced by the immune status of the host. Both histopathological and microbiological assessments are equally important for making a conclusive diagnosis. Anti-fungal therapy may delay the growth of fungi that normally grow within a week; thus, a longer incubation time may be warranted for fungal smear-positive samples.

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Fungal Keratitis Due to Fusarium lichenicola: A Case Report and Global Review of Fusarium lichenicola Keratitis

Journal of Fungi ◽

10.3390/jof7110889 ◽

2021 ◽

Vol 7 (11) ◽

pp. 889

Author(s):

Isra Halim ◽

Prabhakar Singh ◽

Asim Sarfraz ◽

Prathyusha Kokkayil ◽

Binod Kumar Pati ◽

...

Keyword(s):

Case Report ◽

Fusarium Solani ◽

Species Complex ◽

Fusarium Species ◽

Fungal Keratitis ◽

Human Pathogen ◽

Review Of The Literature ◽

Genus Fusarium ◽

The Past ◽

Established Plant

Fusarium species are among the most commonly isolated causes of fungal keratitis. Most species of the genus Fusarium belong to Fusarium solani species complex (FSSC). Fusarium lichenicola, a member of the FSSC complex, is a well-established plant and human pathogen. However, reports of fungal keratitis due to Fusarium lichenicola have not been frequently reported. To the best of our knowledge, only twelve cases of Fusarium lichenicola keratitis have been reported in the past fifty years. Clinical cases of Fusarium lichenicola may have most likely been misidentified because of the lack of clinical and microbiological suspicion, as well as inadequate diagnostic facilities in many tropical countries where the burden of the disease may be the highest. We report a case of fungal keratitis caused by Fusarium lichenicola and present a global review of the literature of all cases of fungal keratitis caused by this potentially blinding fungus.

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Efficacy of Luliconazole Against Broad-Range Filamentous Fungi Including Fusarium solani Species Complex Causing Fungal Keratitis

Cornea ◽

10.1097/ico.0000000000001812 ◽

2018 ◽

pp. 1 ◽

Cited By ~ 4

Author(s):

Daisuke Todokoro ◽

Takashi Suzuki ◽

Takashi Tamura ◽

Koichi Makimura ◽

Hideyo Yamaguchi ◽

...

Keyword(s):

Filamentous Fungi ◽

Fusarium Solani ◽

Species Complex ◽

Fungal Keratitis ◽

Fusarium Solani Species Complex

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Leiomyosarcoma arising from the right ovarian vein

Journal of Surgical Case Reports ◽

10.1093/jscr/rjz302 ◽

2019 ◽

Vol 2019 (11) ◽

Author(s):

Kazuomi Suzuki ◽

Kiyoaki Taniguchi ◽

Shunichi Ito ◽

Akiko Serizawa ◽

Masakazu Yamamoto

Keyword(s):

Computed Tomography ◽

Abdominal Pain ◽

Surgical Approach ◽

Poor Prognosis ◽

Multimodal Therapy ◽

Lung Metastases ◽

Delayed Diagnosis ◽

Pathological Examination ◽

Ovarian Vein ◽

The Right

Abstract Leiomyosarcomas (LMS) of the ovarian vein are extremely rare and have a poor prognosis. Only 10 cases have been reported since 1977. The patient is a 69-year-old woman presented with right abdominal pain. Computed tomography showed a regularly shaped tumor, 80 mm in diameter in the retroperitoneum, adjacent to the descending part of the duodenum. Intraoperatively, the right ovarian vein was found to run through the tumor and was, therefore, resected together with the tumor. Pathological examination revealed a LMS of the right ovarian vein. Nine months postoperatively, multiple lung metastases were detected and chemotherapy was initiated. Delayed diagnosis is associated with high mortality. It is important that the diagnosis of LMS should be made preoperatively when you have already diagnosed a tumor to better direct the surgical approach. Multimodal therapy may improve prognosis.

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