scholarly journals Enhanced Expression of Thaumatin-like Protein Gene (LeTLP1) Endows Resistance to Trichoderma atroviride in Lentinula edodes

Life ◽  
2021 ◽  
Vol 11 (8) ◽  
pp. 863
Author(s):  
Xiaolong Ma ◽  
Xiaolin Fan ◽  
Gangzheng Wang ◽  
Ruiping Xu ◽  
Lianlian Yan ◽  
...  
Keyword(s):  
Yield Loss ◽  
Lentinula Edodes ◽  
Protein Gene ◽  
Trichoderma Atroviride ◽  
Major Mechanism ◽  
Qrt Pcr ◽  
Evaluation Test ◽  
Shiitake Mushrooms ◽  
Important Approach ◽  
Enhanced Expression

Lentinula edodes (shiitake mushrooms) is heavily affected by the infection of Trichoderma atroviride, causing yield loss and decreases quality in shiitake mushrooms. The selection and breeding of fungal-resistant L. edodes species are an important approach to protecting L. edodes from T. atroviride infection. Herein, a highly resistant L. edodes strain (Y3334) and a susceptible strain (Y55) were obtained by using a resistance evaluation test. Transcriptome analyses and qRT-PCR detection showed that the expression level of LeTLP1 (LE01Gene05009) was strongly induced in response to T. atroviride infection in the resistant Y3334. Then, LeTLP1-silenced and LeTLP1-overexpression transformants were obtained. Overexpression of LeTLP1 resulted in resistance to T. atroviride. Compared with the parent strain Y3334, LeTLP1-silenced transformants had reduced resistance relative to T. atroviride. Additionally, the LeTLP1 protein (Y3334) exhibited significant antifungal activity against T. atroviride. These findings suggest that overexpression of LeTLP1 is a major mechanism for the resistance of L. edodes to T. atroviride. The molecular basis provides a theoretical basis for the breeding of resistant L. edodes strains and can eventually contribute to the mushroom cultivation industry and human health.

scholarly journals West Nile Virus Mosquito Vectors (Diptera: Culicidae) in Germany

Viruses ◽  
2020 ◽  
Vol 12 (5) ◽  
pp. 493 ◽  
Author(s):  
Helge Kampen ◽  
Cora M. Holicki ◽  
Ute Ziegler ◽  
Martin H. Groschup ◽  
Birke Andrea Tews ◽  
...  
Keyword(s):  
West Nile Virus ◽  
Virus Strain ◽  
Envelope Protein ◽  
Protein Gene ◽  
Viral Envelope ◽  
Mosquito Vectors ◽  
Viral Envelope Protein ◽  
West Nile ◽  
Qrt Pcr ◽  
First Time

In 2018, West Nile virus (WNV) broke out for the first time in Germany, with continuation of the epidemic in 2019, involving birds, horses and humans. To identify vectors and characterize the virus, mosquitoes were collected in both years in zoological gardens and on a horse meadow immediately following the diagnosis of disease cases in birds and horses. Mosquitoes were identified and screened for WNV by qRT-PCR, with virus-positive samples being sequenced for the viral envelope protein gene. While no positive mosquitoes were found in 2018, seven mosquito pools tested positive for WNV in 2019 in the Tierpark (Wildlife Park) Berlin. The pools consisted of Cx. pipiens biotype pipiens (n = 5), and a mixture of Cx. p. biotype pipiens and Cx. p. biotype molestus (n = 2), or hybrids of these, and were collected between 13 August and 24 September 2019. The virus strain turned out to be nearly identical to two WNV strains isolated from birds diseased in 2018 in eastern Germany. The findings represent the first demonstration of WNV in mosquitoes in Germany and include the possibility of local overwintering of the virus.

scholarly journals Selection and Validation of Reference Genes for qRT-PCR in Lentinula edodes under Different Experimental Conditions

Genes ◽  
2019 ◽  
Vol 10 (9) ◽  
pp. 647 ◽  
Author(s):  
Yi Luo ◽  
Gangzheng Wang ◽  
Chen Wang ◽  
Yuhua Gong ◽  
Yinbing Bian ◽  
...  
Keyword(s):  
Reference Gene ◽  
Reference Genes ◽  
Lentinula Edodes ◽  
Polymerase Chain Reaction Analysis ◽  
Pairwise Variation ◽  
Accurate Estimation ◽  
Expression Stability ◽  
Experimental Conditions ◽  
Qrt Pcr ◽  
Accurate Normalization

Lentinula edodes is the most consumed mushroom in Asia due to its nutritional and medicinal values, and the optimal reference gene is crucial for normalization of its gene expression analysis. Here, the expression stability of 18 candidate reference genes (CRGs) in L. edodes was analyzed by three statistical algorithms (geNorm, NormFinder and BestKeeper) under different stresses (heat, cadmium excess and Trichoderma atroviride infection), different substrates (straw, sawdust and corn stalk) and different development stages (mycelia, primordia and fruit bodies). Among the 18 CRGs, 28S, Actin and α-tub exhibited the highest expression stability in L. edodes under all conditions, while GPD, SPRYP and MSF showed the least stable expression. The best reference gene in different conditions was different. The pairwise variation values showed that two genes would be sufficient for accurate normalization under different conditions of L. edodes. This study will contribute to more accurate estimation of the gene relative expression levels under different conditions using the optimal reference gene in qRT-PCR (quantitative reverse transcription polymerase chain reaction) analysis.

scholarly journals Mineral supplementation and productivity of the Shiitake mushroom on eucalyptus logs

2004 ◽  
Vol 61 (3) ◽  
pp. 260-265 ◽  
Author(s):  
Elvio Cardoso Queiroz ◽  
Regina Helena Marino ◽  
Augusto Ferreira da Eira
Keyword(s):  
Lentinula Edodes ◽  
Shiitake Mushroom ◽  
Mineral Fertilization ◽  
Eucalyptus Saligna ◽  
Mineral Supplement ◽  
Negative Results ◽  
Mineral Supplementation ◽  
Shiitake Mushrooms ◽  
Current Production ◽  
Positive Results

Brazilian reports on Shiitake mushroom [Lentinula edodes (Berkeley) Pegler] productivity grown on Eucalyptus saligna (Sm.) logs are optimistic. The current production on this kind of wood is, however, low and variable. Aiming at increasing Shiitake mushroom productivity, the present work studied interactions among three concentrations of mineral supplement and three Shiitake strains (Le 95/01, 96/17 and 96/18) grown on seventh cut eucalyptus logs. Mineral fertilization was carried out when the logs were submerged in water to induce fruiting bodies, using the following saline concentrations: zero (no fertilizers added), 0.05% (equivalent to 0.35 g L-1 of ammonium sulfate with 18% nitrogen and 0.15 g L-1 of superphosphate with 34% phosphorus) and 0.50%. Fertilization of the Le 95/01 strain increased productivity, but logs inoculated with the Le 96/17 strain were more productive only at 0.05% concentration while in the Le 96/18 strain, mineral supplementation decreased in productivity. The radial growth of these Shiitake strains (in sawdust-agar medium and with the same saline concentrations used in the log experiment) showed that all strains reacted positively to fertilization and that positive results for the Le 95/01 strain and negative results for Le 96/18 on eucalyptus logs are due to the natural higher competitiveness of the first Le 95/01 in relation to the latter Le 96/18 strain. Mineral fertilization increases the productivity provided that the logs are well colonized by Shiitake mushrooms.

scholarly journals (395) Greater Inoculation Rate Enhances Biological Efficiency and Returns of Shiitake Mushrooms

HortScience ◽  
2005 ◽  
Vol 40 (4) ◽  
pp. 1034A-1034
Author(s):  
Cathy M. Sabota ◽  
Caula A. Beyl
Keyword(s):  
Lentinula Edodes ◽  
Biological Efficiency ◽  
White Oak ◽  
Net Profit ◽  
Shiitake Mushrooms ◽  
Inoculation Treatment

To determine if biological efficiency of shiitake mushrooms was affected by the density of spawn inoculation, white oak logs about 31 inches (0.79 m) long were cut from trees harvested 14 Apr. and inoculated 6 May 2003 with strain WR46 of Lentinula edodes using four rates of inoculation. The number of holes drilled and inoculated with spawn was determined by multiplying the weight of each log by 0.5, 1, 2, or 3. A 15-pound log (6.8 kg) inoculated at “3” times the weight would have 45 holes drilled and inoculated. Each rate of inoculation treatment was replicated three times with three subsamples per replication. After six harvests over a 21-month period, the biological efficiency (BE) of the logs increased by 1.94% per additional hole per pound (0.45 kg). This increase in BE would result in $1.15 in additional returns per log with a cost of only $0.15 more per log due to increased spawn use, resulting in a net profit, excluding increased labor and wax costs, of $1.00 per log.

scholarly journals Strain of Shiitake Mushroom [Lentinula edodes (Berk.) Pegler] and Wood Species Affect the Yield of Shiitake Mushrooms

1996 ◽  
Vol 6 (4) ◽  
pp. 388-393 ◽  
Author(s):  
Cathy Sabota
Keyword(s):  
Tree Species ◽  
Lentinula Edodes ◽  
Wholesale Price ◽  
Biological Efficiency ◽  
Small Scale ◽  
Red Oak ◽  
Shiitake Mushroom ◽  
White Oak ◽  
Shiitake Mushrooms ◽  
The Cost

Logs of white and red oak (Quercus spp.), black cherry [Prunus serotina (Ehrh.)], sassafras (Sassafras albidum (Nutt.) Nees), and eastern sycamore [Platanus occidentalis (L.)] were inoculated with eight strains of shiitake mushroom in 12-mm holes drilled 25 mm deep and spaced 15 cm apart down the log and 5 cm apart around the log in a staggered pattern. Logs then were placed in quonset houses covered with 80% shadecloth in Mar. 1991. The environment within these shaded houses was not modified otherwise. Harvested mushrooms were counted and weighed and biological efficiency (BE) was determined. White and red oaks produced significantly higher yields of shiitake mushrooms over the lifetime of the log than the other types of wood. Only one strain, CW25, produced significantly lower yields than the best four strains. Interactions among strains and seasons of harvest and the species and seasons of harvest were significant. The most productive mushroom strains and tree species yielded more mushrooms later in the life of the logs than the least productive strains and tree species did earlier in the life of the logs. BE attained from strain × species interactions ranged from 0% (WW70, CW25, and WR85 on sycamore) to 8.8% (WW44 on red oak) in an outdoor noncontrolled environment. The gross per cord value (at wholesale price) of shiitake harvested from white oak was $2475.00. The cost of producing one cord of shiitake is about $6.44 per log in a small-scale operation. A net return of $1509.00 per cord is realistic and includes the cost of labor.

The quantitative relationships among alkali elements and the availability of Rb as a substitute for radioactive Cs in Lentinula edodes

2018 ◽  
Vol 28 (01n02) ◽  
pp. 61-68
Author(s):  
A. Terakawa ◽  
Y. Hirakata ◽  
K. Kikuchi ◽  
A. Fujita ◽  
T. Matsuyama ◽  
...  
Keyword(s):  
Spatial Distribution ◽  
Lentinula Edodes ◽  
Peripheral Region ◽  
Shiitake Mushroom ◽  
Emission Analysis ◽  
Pixe Analysis ◽  
X Ray ◽  
Shiitake Mushrooms ◽  
Alkali Elements ◽  
Mushroom Sample

We performed particle-induced X-ray emission analysis to determine the quantitative relationships among alkali elements including radioactive Cs in Lentinula edodes (shiitake mushroom). The shiitake mushrooms were cultivated using radioactive Cs-contaminated hardwood logs, with Rb and stable Cs added to evaluate their concentrations by particle-induced X-ray emission (PIXE) analysis, as well as to measure the spatial distribution of radioactive Cs in the mushroom sample using an autoradiographic method. Radioactive Cs were strongly concentrated in the peripheral region of the mushroom pileus, whereas K, Rb, and stable Cs highly accumulated in the same region as radioactive Cs. We confirmed that the concentrations of K and Rb were basically proportional to that of stable Cs. In particular, a clear linear correlation was observed between the Rb and Cs concentrations. The results of this study indicate the availability of Rb as a substitute for Cs to study the accumulation of radioactive Cs in mushrooms.

scholarly journals Selection of Reference Genes for qRT-PCR Analysis in Lentinula edodes after Hot-Air Drying

Molecules ◽  
2018 ◽  
Vol 24 (1) ◽  
pp. 136 ◽  
Author(s):  
Shuangshuang Gao ◽  
Gangzheng Wang ◽  
Zhicheng Huang ◽  
Xiaoyu Lei ◽  
Yinbing Bian ◽  
...  
Keyword(s):  
Fruiting Body ◽  
Reference Genes ◽  
Lentinula Edodes ◽  
Sulfur Compounds ◽  
Pcr Analysis ◽  
Expression Stability ◽  
Hot Air Drying ◽  
Air Drying ◽  
Qrt Pcr ◽  
Hot Air

Volatile sulfur compounds gradually develop in Lentinula edodes after hot-air drying, and many genes are involved in the generation of these sulfur compounds. The expression stability of reference genes may vary in a particular experimental treatment when analyzing their expressions by quantitative real-time polymerase chain reaction (qRT-PCR). In this study, the expression profile of 17 candidate genes was assessed in L. edodes under treatment at 50 °C for 0, 1, 2, and 3 h, and the expression stability of each reference gene was analyzed by three statistical algorithms, including geNorm, NormFinder, and BestKeeper. Results indicated that the two optimal reference genes for mycelium and fruiting body were CAC and DAHP as well as CAC and NUP, respectively. Additionally, CAC and DAHP were found to be the two most stable reference genes across the mycelium and fruiting body set. Our results will provide a genetic foundation for further research on the metabolism genes of sulfur compounds in L. edodes.

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