Effect of the Compositions on the Biocompatibility of New Alumina–Zirconia–Titania Dental Ceramic Composites

Dental implant biomaterials are expected to be in contact with living tissues, therefore their toxicity and osseointegration ability must be carefully assessed. In the current study, the wettability, cytotoxicity, and genotoxicity of different alumina–zirconia–titania composites were evaluated. The surface wettability determines the biological event cascade in the bioceramic/human living tissues interface. The measured water contact angle indicated that the wettability strongly depends on the ceramic composition. Notwithstanding the contact angle variability, the ceramic surfaces are hydrophilic. The cytotoxicity of human gingival fibroblast cells with materials, evaluated by an (3-(4,5 methylthiazol-2-yl)-2,5-diphenyl-tetrazolium bromide (MTT) test, revealed an absence of any cytotoxic effect. A relationship was found between the cell viability and the wettability. It was subsequently deduced that the cell viability increases when the wettability increases. This effect is more pronounced when the titania content is higher. Finally, a comet test was applied as complementary biocompatibility test to detect any changes in fibroblast cell DNA. The results showed that the DNA damage is intimately related to the TiO2 content. Genotoxicity was mainly attributed to ceramic composites containing 10 wt.% TiO2. Our research revealed that the newly developed high performance alumina–zirconia–titania ceramic composites contain less than 10 wt.% TiO2, and display promising surface properties, making them suitable for dental implantology applications.

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Cytotoxic Evaluation of Mala aluation of Malaysian K ysian Kelulut Hone elulut Honey on Human y on Human Gingival Fibr al Fibroblast Cell Line using M oblast Cell Line using MTT Assay

Journal of Dentistry Indonesia ◽

10.14693/jdi.v28i2.1244 ◽

2021 ◽

Vol 28 (2) ◽

Author(s):

Chee Zi Yun ◽

◽

Nurul Hafizah Mohd Nor ◽

Zurairah Berahim ◽

Kannan Thirumulu Ponnuraj ◽

...

Keyword(s):

Cell Viability ◽

Cell Line ◽

Mtt Assay ◽

Fibroblast Cell ◽

P Value ◽

Human Gingival Fibroblast ◽

Growth Media ◽

Gingival Fibroblast ◽

Fibroblast Cell Line ◽

Time Points

Kelulut honey or stingless bee honey is a type of honey produced by stingless bees of the Trigona species where the nest is found in living trees. Objective: The aim of this study was to evaluate the cytotoxic potential of Malaysian Kelulut honey by employing MTT assay on a human gingival fibroblast cell line. Methods: Human gingival fibroblast cell line was cultured in minimal essential medium alpha (α-MEM) with 10% foetal bovine serum and 1% penicillin-streptomycin solution in a 5% CO2 incubator at 37°C in a humidified atmosphere. The cells were seeded at a cell density of 5x103 cells/well in a 96-well culture plate for 24 hours. The cells were treated with seven different concentrations (200, 100, 50, 25, 12.5, 6.25, and 3.125mg/ml) of Malaysian Kelulut honey and incubated in a CO2 incubator. The negative control comprised cells treated with growth media alone. The cell viability was assessed using MTT assay at 24, 48, and 72 hours. The test plate was shaken using a microplate shaker and the absorbance of the solution was measured at 570nm using an ELISA reader with the Magellan software. Statistical analysis of the data was carried out using Kruskal-Wallis test and SPSS 24.0.0 for Windows. A p value <0.05 was considered as statistically significant. Results: There was no cytotoxic effect of Malaysian Kelulut honey on HGF-1 based on the MTT assay at different concentrations and at different time points tested as the cell viability was above 70%. The highest percentage of cell viability at all three different durations of treatment were observed at 3.125mg/ml, whereas the lowest cell viability was observed at 200mg/ml of Kelulut honey concentration. However, statistically significant differences were seen between some of the concentrations at various time points. Conclusion: Since the cell viability of HGF-1 treated with Malaysian Kelulut honey was more than 70% at all concentrations ranging from 3.125mg/ml to 200mg/ml at three different time points (24, 48 and 72 hours), Malaysian Kelulut honey can be considered as non-cytotoxic on human gingival fibroblasts based on MTT assay under the present test conditions

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Glutathione-Stabilized Silver Nanoparticles: Antibacterial Activity against Periodontal Bacteria, and Cytotoxicity and Inflammatory Response in Oral Cells

Biomedicines ◽

10.3390/biomedicines8100375 ◽

2020 ◽

Vol 8 (10) ◽

pp. 375

Author(s):

Irene Zorraquín-Peña ◽

Carolina Cueva ◽

Dolores González de Llano ◽

Begoña Bartolomé ◽

M. Victoria Moreno-Arribas

Keyword(s):

Antimicrobial Activity ◽

Silver Nanoparticles ◽

Inflammatory Response ◽

Cell Viability ◽

Antibacterial Properties ◽

Fusobacterium Nucleatum ◽

Fibroblast Cell ◽

Human Gingival Fibroblast ◽

Gingival Fibroblast ◽

Necrosis Factor Alpha

Silver nanoparticles (AgNPs) have been proposed as new alternatives to limit bacterial dental plaque because of their antimicrobial activity. Novel glutathione-stabilized silver nanoparticles (GSH-AgNPs) have proven powerful antibacterial properties in food manufacturing processes. Therefore, this study aimed to evaluate the potentiality of GSH-AgNPs for the prevention/treatment of oral infectious diseases. First, the antimicrobial activity of GSH-AgNPs against three oral pathogens (Porphyromonas gingivalis, Fusobacterium nucleatum, and Streptococcus mutans) was evaluated. Results demonstrated the efficiency of GSH-AgNPs in inhibiting the growth of all bacteria, especially S. mutans (IC50 = 23.64 μg/mL, Ag concentration). Second, GSH-AgNPs were assayed for their cytotoxicity (i.e., cell viability) toward a human gingival fibroblast cell line (HGF-1), as an oral epithelial model. Results indicated no toxic effects of GSH-AgNPs at low concentrations (≤6.16 µg/mL, Ag concentration). Higher concentrations resulted in losing cell viability, which followed the Ag accumulation in cells. Finally, the inflammatory response in the HGF-1 cells after their exposure to GSH-AgNPs was measured as the production of immune markers (interleukins 6 and 8 (IL-6 and IL-8) and tumor necrosis factor-alpha (TNF-α)). GSH-AgNPs activates the inflammatory response in human gingival fibroblasts, increasing the production of cytokines. These findings provide new insights for the use of GSH-AgNPs in dental care and encourage further studies for their application.

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Surface Characterization, Antimicrobial Activity, and Biocompatibility of Autopolymerizing Acrylic Resins Coated with Reynoutria elliptica Extract

Plants ◽

10.3390/plants9101292 ◽

2020 ◽

Vol 9 (10) ◽

pp. 1292

Author(s):

Song-Yi Yang ◽

Min-Kyung Kang

Keyword(s):

Contact Angle ◽

Antimicrobial Activity ◽

Cell Viability ◽

Water Contact Angle ◽

Vickers Hardness ◽

Surface Characterization ◽

Acrylic Resin ◽

Water Contact ◽

Acrylic Resins ◽

Vis Spectroscopy

We conducted surface characterization to assess the biocompatibility and investigate the antimicrobial activity against oral pathogens in autopolymerizing acrylic resins, coated with light-curable coating resin, containing various concentrations of Reynoutria elliptica extract (0, 200, 400, and 600 µg/mL). The R. elliptica extract powder was prepared using a freeze-drying technique. Further, a goniometer and microhardness tester were used to determine the water contact angle, and Vickers hardness, respectively; color measurements were performed on the uncoated and coated acrylic resin disks. The polyphenol content of the extracts from the coated acrylic resin disk was analyzed using UV-VIS spectroscopy. The antimicrobial activity of the coated acrylic resin disk against Streptococcus mutans and Candida albicans was observed for 24 and 48 h by measuring the optical density using spectrophotometry. In addition, biocompatibility was confirmed by testing the cell viability according to ISO 10993-5. The water contact angle, Vickers hardness, and color change values of the coated acrylic resin disks were not significantly different from the control. Polyphenol was detected in all experimental groups, with no significant differences between the experimental groups. The experimental groups exhibited significant antimicrobial activity against S. mutans and C. albicans compared to the control group, after 48 h of incubation. The cell viability between the control and experimental groups was not significantly different. The proposed coating resin containing R. elliptica extract is applicable on dental acrylic resins, due to their antimicrobial properties and excellent biocompatibility, with no deterioration of surface characteristics.

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Human gingival fibroblast cell lines in vitro.

Journal of Periodontal Research ◽

10.1111/j.1600-0765.1980.tb00260.x ◽

1980 ◽

Vol 15 (1) ◽

pp. 53-70 ◽

Cited By ~ 24

Author(s):

George G. Rose ◽

Toshihiko Yajima ◽

Charles J. Mahan

Keyword(s):

Cell Lines ◽

Fibroblast Cell ◽

Human Gingival Fibroblast ◽

Gingival Fibroblast ◽

Fibroblast Cell Lines

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Cytotoxicity of acrylic based resin compounds in a human gingival fibroblast cell line

Revista Portuguesa de Estomatologia Medicina Dentária e Cirurgia Maxilofacial ◽

10.1016/j.rpemd.2013.02.003 ◽

2013 ◽

Vol 54 (2) ◽

pp. 87-90

Author(s):

Mariana Souto-Lopes ◽

Álvaro Azevedo ◽

Alexandra Teixeira ◽

Diana Bastos-Aires ◽

José Lordelo ◽

...

Keyword(s):

Cell Line ◽

Fibroblast Cell ◽

Human Gingival Fibroblast ◽

Gingival Fibroblast ◽

Fibroblast Cell Line

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Establishment of immotalized human gingival fibroblast cell lines

The Journal of the Korean Academy of Periodontology ◽

10.5051/jkape.2002.32.3.603 ◽

2002 ◽

Vol 32 (3) ◽

pp. 603

Author(s):

Jae-Bong Song ◽

Hyun-A Kim ◽

Ha-Na Hyun ◽

Eun-Cheol Kim ◽

Hyung-Keun You ◽

...

Keyword(s):

Cell Lines ◽

Fibroblast Cell ◽

Human Gingival Fibroblast ◽

Gingival Fibroblast ◽

Fibroblast Cell Lines

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Cytotoxicity Evaluation of Two Bis-Acryl Composite Resins Using Human Gingival Fibroblasts

Brazilian Dental Journal ◽

10.1590/0103-6440201600824 ◽

2016 ◽

Vol 27 (5) ◽

pp. 492-496 ◽

Cited By ~ 4

Author(s):

Fabiano Palmeira Gonçalves ◽

◽

Gutemberg Alves ◽

Vladi Oliveira Guimarães Júnior ◽

Marco Antônio Gallito ◽

...

Keyword(s):

Cell Viability ◽

Membrane Integrity ◽

Composite Resins ◽

Human Gingival Fibroblasts ◽

Control Group ◽

Mitochondrial Activity ◽

Human Gingival Fibroblast ◽

Gingival Fibroblast ◽

Gingival Fibroblasts ◽

Xtt Assay

Abstract Bis-acryl resins are used for temporary dental restorations and have shown advantages over other materials. The aim of this work was to evaluate the in vitro cytotoxicity of two bis-acryl composite resins (Protemp 4 and Luxatemp Star), obtained at 1, 7 and 40 days after mixing the resin components, using a standardized assay employing human primary cells closely related to oral tissues. Human gingival fibroblast cell cultures were exposed for 24 h to either bis-acryl composite resins, polystyrene beads (negative control) and latex (positive control) extracts obtained after incubation by the different periods, at 37 °C under 5% CO2. Cell viability was evaluated using a multiparametric procedure involving sequential assessment (using the same cells) of mitochondrial activity (XTT assay), membrane integrity (neutral red test) and total cell density (crystal violet dye exclusion test). The cells exposed to the resin extracts showed cell viability indexes exceeding 75% after 24 h. Even when cells were exposed to extracts prepared with longer conditioning times, the bis-acryl composite resins showed no significant cytotoxic effects (p>0.05), compared to the control group or in relation to the first 24 h of contact with the products. There were no differences among the results obtained for the bis-acryl composite resins evaluated 24 h, 7 days and 40 days after mixing. It may be concluded that the bis-acryl resins Protemp 4 and Luxatemp Star were cytocompatible with human gingival fibroblasts, suggesting that both materials are suitable for use in contact with human tissues.

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Comparison of Cytotoxicity of New Nanohybrid Composite, Giomer, Glass Ionomer and Silver Reinforced Glass Ionomer using Human Gingival Fibroblast Cell Line

Journal of Clinical Pediatric Dentistry ◽

10.17796/1053-4628-41.5.368 ◽

2017 ◽

Vol 41 (5) ◽

pp. 368-373 ◽

Cited By ~ 3

Author(s):

Fatemeh Koohpeima ◽

Mohammad Javad Mokhtari ◽

Maryam Doozandeh ◽

Zahra Jowkar ◽

Fatemeh Yazdanshenas

Keyword(s):

Cytotoxic Effect ◽

Dental Materials ◽

Fibroblast Cell ◽

Human Gingival Fibroblast ◽

Gingival Fibroblast ◽

Glass Ionomer ◽

Cell Culture Study ◽

Wide Range ◽

Resin Modified Glass Ionomer ◽

Nanohybrid Composite

Aim: The objective of this study was to investigate the cytotoxic effects of new nanohybrid composite, giomer, conventional and resin modified and silver reinforced glass ionomer cements and compare the biocompatibility of these dental materials in cell culture. Study design: Five cylindrical specimens were made of each material, using a mold (2mm. thick and 5 mm in diameter). For HGF, cells were cultured in RPMI-1640 medium. After attaining 80% confluence, cells were treated with different doses of five tested materials for 24h. Then cell cytotoxicity was assessed using MTT assay. The data were analyzed using Kruskal-Wallis and Dunn test. Results: The materials evaluated on HGF cells, showed significantly more cytotoxicity in silver reinforced glass ionomer but nanohybrid composite shows mild cytotoxic effect. However, giomer shows no significant cytotoxicity and conventional and resin modified glass ionomer enhance cell proliferation. Conclusions: Silver reinforced glass ionomer induced a significant high cytotoxic effect over a wide range of concentration. Therefore, higher attention should be focused on this restorative dental material, which should be chosen for further investigations.

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Rinsing with L-Ascorbic Acid Exhibits Concentration-Dependent Effects on Human Gingival Fibroblast In Vitro Wound Healing Behavior

International Journal of Dentistry ◽

10.1155/2020/4706418 ◽

2020 ◽

Vol 2020 ◽

pp. 1-7

Author(s):

Tatcha Chaitrakoonthong ◽

Ruchanee Ampornaramveth ◽

Paksinee Kamolratanakul

Keyword(s):

Wound Healing ◽

Ascorbic Acid ◽

Vitamin C ◽

Cell Viability ◽

Real Time ◽

Human Gingival Fibroblast ◽

Gingival Fibroblast ◽

Fibroblast Migration ◽

Matrix Gene

Vitamin C or L-ascorbic acid has diverse functions in the body, especially healing promotion in tissue injury via participating in the hydroxylation reactions required for collagen formation. Systemic administration of vitamin C plays an important role on gingival fibroblast proliferation and functions. Whether local or rinsing administration of vitamin C alters gingival fibroblast wound healing behavior remains unclear. The aim of this study was to investigate the rinsing effect of vitamin C on gingival fibroblast behavior utilizing an in vitro wound healing model. Primary human gingival fibroblasts isolated from gingival tissue were rinsed with medium containing various concentrations of vitamin C. The rinsing effect of vitamin C on in vitro wound healing was assessed using a scratch test assay. Cell migration, cell viability, and extracellular matrix gene expression were analyzed by transwell migration assay, MTT assay, and real-time RT-PCR, respectively. We found that rinsing with 10 or 20 µg/ml vitamin C significantly increased fibroblast migration (p≤0.05). However, no significant effect was found in the cell viability or in vitro wound healing assays. In contrast, rinsing with 50 µg/ml vitamin C significantly delayed wound closure (p≤0.05). Real-time PCR demonstrated that 50 µg/ml vitamin C significantly increased fibroblast expression of COL1, FN, IL-6, and bFGF. The data demonstrate that rinsing with vitamin C (10/20 µg/ml) accelerates fibroblast migration. However, 50 µg/ml of vitamin C increases the expression of COL1, FN, IL-6, and bFGF, which are related to fibroblast wound healing activity. Prescribing vitamin C with the appropriate duration and drug administration method should be determined to maximize its benefit.

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