Comparison of Cytotoxicity of New Nanohybrid Composite, Giomer, Glass Ionomer and Silver Reinforced Glass Ionomer using Human Gingival Fibroblast Cell Line

2017 ◽  
Vol 41 (5) ◽  
pp. 368-373 ◽  
Author(s):  
Fatemeh Koohpeima ◽  
Mohammad Javad Mokhtari ◽  
Maryam Doozandeh ◽  
Zahra Jowkar ◽  
Fatemeh Yazdanshenas
Keyword(s):  
Cytotoxic Effect ◽  
Dental Materials ◽  
Fibroblast Cell ◽  
Human Gingival Fibroblast ◽  
Gingival Fibroblast ◽  
Glass Ionomer ◽  
Cell Culture Study ◽  
Wide Range ◽  
Resin Modified Glass Ionomer ◽  
Nanohybrid Composite

Aim: The objective of this study was to investigate the cytotoxic effects of new nanohybrid composite, giomer, conventional and resin modified and silver reinforced glass ionomer cements and compare the biocompatibility of these dental materials in cell culture. Study design: Five cylindrical specimens were made of each material, using a mold (2mm. thick and 5 mm in diameter). For HGF, cells were cultured in RPMI-1640 medium. After attaining 80% confluence, cells were treated with different doses of five tested materials for 24h. Then cell cytotoxicity was assessed using MTT assay. The data were analyzed using Kruskal-Wallis and Dunn test. Results: The materials evaluated on HGF cells, showed significantly more cytotoxicity in silver reinforced glass ionomer but nanohybrid composite shows mild cytotoxic effect. However, giomer shows no significant cytotoxicity and conventional and resin modified glass ionomer enhance cell proliferation. Conclusions: Silver reinforced glass ionomer induced a significant high cytotoxic effect over a wide range of concentration. Therefore, higher attention should be focused on this restorative dental material, which should be chosen for further investigations.

Human gingival fibroblast cell lines in vitro.

1980 ◽  
Vol 15 (1) ◽  
pp. 53-70 ◽  
Author(s):  
George G. Rose ◽  
Toshihiko Yajima ◽  
Charles J. Mahan
Keyword(s):  
Cell Lines ◽  
Fibroblast Cell ◽  
Human Gingival Fibroblast ◽  
Gingival Fibroblast ◽  
Fibroblast Cell Lines

scholarly journals Cytotoxicity of acrylic based resin compounds in a human gingival fibroblast cell line

2013 ◽  
Vol 54 (2) ◽  
pp. 87-90
Author(s):  
Mariana Souto-Lopes ◽  
Álvaro Azevedo ◽  
Alexandra Teixeira ◽  
Diana Bastos-Aires ◽  
José Lordelo ◽  
...  
Keyword(s):  
Cell Line ◽  
Fibroblast Cell ◽  
Human Gingival Fibroblast ◽  
Gingival Fibroblast ◽  
Fibroblast Cell Line

scholarly journals Comparison of microleakage of an alkasite restorative material, a composite resin and a resin-modified glass ionomer

2021 ◽  
Vol 20 ◽  
pp. e213981
Author(s):  
Fariba Motevasselian ◽  
Hamid Kermanshah ◽  
Ebrahim Rasoulkhani ◽  
Mutlu Özcan
Keyword(s):  
Composite Resin ◽  
Composite Resins ◽  
Glass Ionomer ◽  
Etch And Rinse ◽  
Significant Difference ◽  
Resin Modified Glass Ionomer ◽  
Group A ◽  
Nanohybrid Composite ◽  
Group B ◽  
Group D

Aim: To compare the microleakage of Cention N, a subgroup of composite resins with a resin-modified glass ionomer (RMGI) and a composite resin. Methods: Class V cavities were prepared on the buccal and lingual surfaces of 46 extracted human molars. The teeth were randomly assigned to four groups. Group A: Tetric N-Bond etch-and-rinse adhesive and Tetric N-Ceram nanohybrid composite resin, group B: Cention N without adhesive, group C: Cention N with adhesive, and group D: Fuji II LC RMGI. The teeth were thermocycled between 5°-55°C (×10,000). The teeth were coated with two layers of nail vanish except for 1 mm around the restoration margins, and immersed in 2% methylene blue (37°C, 24 h) before buccolingual sectioning to evaluate dye penetration under a stereomicroscope (×20). The data were analyzed by the Kruskal-Wallis and Wilcoxon tests (α=0.05). Results: Type of material and restoration margin had significant effects on the microleakage (p<0.05). Dentin margins showed a higher leakage score in all groups. Cention N and RMGI groups showed significant differences at the enamel margin (p=0.025, p=0.011), and for the latter group the scores were higher. No significant difference was found at the dentin margins between the materials except between Cention N with adhesive and RMGI (p=0.031). Conclusion: Microleakage was evident in all three restorative materials. Cention N groups showed similar microleakage scores to the composite resin and displayed lower microleakage scores compared with RMGI.

Establishment of immotalized human gingival fibroblast cell lines

2002 ◽  
Vol 32 (3) ◽  
pp. 603
Author(s):  
Jae-Bong Song ◽  
Hyun-A Kim ◽  
Ha-Na Hyun ◽  
Eun-Cheol Kim ◽  
Hyung-Keun You ◽  
...  
Keyword(s):  
Cell Lines ◽  
Fibroblast Cell ◽  
Human Gingival Fibroblast ◽  
Gingival Fibroblast ◽  
Fibroblast Cell Lines

Human gingival fibroblast cell lines in vitro.

1980 ◽  
Vol 15 (3) ◽  
pp. 267-287 ◽  
Author(s):  
Toshihiko Yajima ◽  
George G. Rose ◽  
Charles J. Mahan
Keyword(s):  
Cell Lines ◽  
Fibroblast Cell ◽  
Human Gingival Fibroblast ◽  
Gingival Fibroblast ◽  
Fibroblast Cell Lines

scholarly journals Propolis nanoparticle enhances the potency of antimicrobial photodynamic therapy against Streptococcus mutans in a synergistic manner

2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Shima Afrasiabi ◽  
Maryam Pourhajibagher ◽  
Nasim Chiniforush ◽  
Abbas Bahador
Keyword(s):  
Photodynamic Therapy ◽  
Streptococcus Mutans ◽  
Biofilm Formation ◽  
Synergistic Effects ◽  
Fibroblast Cell ◽  
Human Gingival Fibroblast ◽  
Gingival Fibroblast ◽  
Antimicrobial Photodynamic Therapy ◽  
The Individual

Abstract Less invasive removal approaches have been recommended for deep caries lesions. Antimicrobial photodynamic therapy (aPDT) and propolis nanoparticle (PNP) are highlighted for the caries management plan. Evidence is lacking for an additive effect of combination PNP with photosensitizer (PS) in aPDT. This study aimed to investigate the individual and synergistic effects of chlorophyllin-phycocyanin mixture (PhotoActive+) and toluidine blue O (TBO) as PSs in combination with PNP in the aPDT process (aPDTplus) against major important virulence factors of Streptococcus mutans. Following characterization, biocompatibility of the PSs alone, or in combination with PNP were investigated on human gingival fibroblast cell. The in vitro synergy of PhotoActive+ or TBO and PNP was evaluated by the checkerboard method. The bacteria's virulence properties were surveyed in the presence of the PSs, individually as well as in combination. When the PSs were examined in combination (synergistic effect, FIC Index < 0.5), a stronger growth inhibitory activity was exhibited than the individual PSs. The biofilm formation, as well as genes involved in biofilm formation, showed greater suppression when the PSs were employed in combination. Overall, the results of this study suggest that the combination of PhotoActive+ or TBO with PNP with the least cytotoxicity effects and the highest antimicrobial activites would improve aPDT outcomes, leading to synergistic effects and impairing the virulence of S. mutans.

scholarly journals Antimicrobial and cytotoxic activities of Barleria prionitis and Barleria grandiflora: A comparative study

2016 ◽  
Vol 11 (4) ◽  
pp. 802
Author(s):  
Hemant Arunrao Sawarkar ◽  
Pranita P. Kashyap ◽  
Ajit Kumar Pandey ◽  
Mukesh Kumar Singh ◽  
Chanchal Deep Kaur
Keyword(s):  
Dermal Fibroblast ◽  
Video Clip ◽  
Ethanolic Extract ◽  
Fibroblast Cell ◽  
Human Dermal Fibroblast ◽  
Human Gingival Fibroblast ◽  
Cytotoxic Activities ◽  
Gingival Fibroblast ◽  
Rural People ◽  
Fibroblast Cell Lines

<p class="Abstract">Leaf juices as well as leaves of <em>Barleria prionitis</em> and <em>Barleria grandiflora</em> are being used by rural people across various regions of India in the treatment of oral ailments such as dental troubles, gum ailments, pyorrhoea, dental carries and mouth ulcers. Zone of inhibition and MIC values obtained for all the extracts suggest ethanolic extract of the herbs were more antimicrobial when compared to the aqueous extract. Results of biofilm suppression were found statistically significant (p&lt;0.05) when compared to control. Cytotoxicity was evaluated by MTT assay on human gingival fibroblast and human dermal fibroblast cell lines for ethanolic extract of the herbs. CTC<sub>50</sub> value was found to be more than 1,000 µg/mL for ethanolic extracts of both herbs. Chlorhexidine was found to be more cytotoxic with CTC<sub>50 </sub>value of 12.5–25 µg/mL. Ethanolic extract of <em>B. prionitis</em> and <em>B. grandiflora</em> found significantly cytotoxic (p&lt;0.05) in comparison with control.</p><p class="Abstract"><strong>Video Clip:</strong></p><p class="Abstract"><a href="https://youtube.com/v/DNByJJQT3RY">Cytotoxicity assay:</a> 18 min 45 sec</p><p> </p>

Microscopic Assay for the Phagocytotic-Collagenolytic Performance (PCP Index) of Human Gingival Fibroblasts in vitro

1978 ◽  
Vol 57 (11-12) ◽  
pp. 1003-1015 ◽  
Author(s):  
George G. Rose ◽  
Toshihiko Yajima ◽  
Charles J. Mahan
Keyword(s):  
Tissue Culture ◽  
Thin Layer ◽  
Cell Lines ◽  
Fibroblast Cell ◽  
Human Gingival Fibroblast ◽  
Gingival Fibroblast ◽  
Gingival Fibroblasts ◽  
Cover Slip ◽  
Fibroblast Cell Lines

Using 16 human gingival fibroblast cell lines from patients with periodontitis, Dilantin hyperplasia, and nonpathological gingiva, a microscopic assay was developed to quantitate the cells' ability to lyse collagen substrates. The method employs tissue culture chambers with one cover slip partially coated with a thin layer of undenatured fibrillar bovine codlagen. The assay measures the relative numbers and sizes of holes in the collagen within defined regions of the cover slips effected by the phagocytotic and collagenolytic performance (PCP) of the population of fibroblasts growing on the cover slip for 5 days. The effect on the PCP index by serum, heparin, prostaglandins, and endotoxin was evaluated.

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