scholarly journals Xeno-Free In Vitro Cultivation and Osteogenic Differentiation of hAD-MSCs on Resorbable 3D Printed RESOMER®

Materials ◽  
2020 ◽  
Vol 13 (15) ◽  
pp. 3399
Author(s):  
Marline Kirsch ◽  
Annabelle-Christin Herder ◽  
Cécile Boudot ◽  
Andreas Karau ◽  
Jessica Rach ◽  
...  
Keyword(s):  
Osteogenic Differentiation ◽  
Autologous Transplantation ◽  
Calf Serum ◽  
Human Mesenchymal Stem Cells ◽  
In Vitro Cultivation ◽  
Human Mscs ◽  
In Vitro Biocompatibility ◽  
Human Platelet Lysate ◽  
3D Printed

The development of alloplastic resorbable materials can revolutionize the field of implantation technology in regenerative medicine. Additional opportunities to colonize the three-dimensionally (3D) printed constructs with the patient’s own cells prior to implantation can improve the regeneration process but requires optimization of cultivation protocols. Human platelet lysate (hPL) has already proven to be a suitable replacement for fetal calf serum (FCS) in 2D and 3D cell cultures. In this study, we investigated the in vitro biocompatibility of the printed RESOMER® Filament LG D1.75 materials as well as the osteogenic differentiation of human mesenchymal stem cells (hMSCs) cultivated on 3D printed constructs under the influence of different medium supplements (FCS, human serum (HS) and hPL). Additionally, the in vitro degradation of the material was studied over six months. We demonstrated that LG D1.75 is biocompatible and has no in vitro cytotoxic effects on hMSCs. Furthermore, hMSCs grown on the constructs could be differentiated into osteoblasts, especially supported by supplementation with hPL. Over six months under physiological in vitro conditions, a distinct degradation was observed, which, however, had no influence on the biocompatibility of the material. Thus, the overall suitability of the material LG D1.75 to produce 3D printed, resorbable bone implants and the promising use of hPL in the xeno-free cultivation of human MSCs on such implants for autologous transplantation have been demonstrated.

Nanofibrous electrospun barrier membrane promotes osteogenic differentiation of human mesenchymal stem cells

2011 ◽  
Vol 26 (6) ◽  
pp. 607-618 ◽  
Author(s):  
Ping Wang ◽  
Ping Gong ◽  
Yi Lin ◽  
Yili Qu ◽  
Jidong Li ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Osteogenic Differentiation ◽  
Fibrous Tissue ◽  
Human Mesenchymal Stem Cells ◽  
Negative Influence ◽  
Electrospun Membrane ◽  
In Vitro Biocompatibility ◽  
Barrier Membrane

An electrospun polysulfone (PSU) was prepared as a barrier membrane for guided bone regeneration. The membrane was in nanoscale to prevent fibrous tissue infiltration and highly porous to allow permeation of oxygen and nutrients. The morphology and attachment, viability and proliferation, and differentiation and mineralization of human bone marrow mesenchymal stem cells (HBMSCs) were determined. Cells adhered and spread well on the PSU membrane with characteristic polygonal, fusiform shapes and radial extensions. The live/dead staining revealed that the membrane had no negative influence on cell viability. The proliferation rates of HBMSCs on PSU membranes were lower in comparison with tissue-culture polystyrene plate after 3 days of culture. However, differentiation activity was particularly expressed at high levels when cells were cultured on PSU membranes. The results based on the data suggest that the PSU electrospun membrane promoted the osteogenic differentiation of HBMSCs, displayed desirable in vitro biocompatibility, and has good potential as a barrier membrane.

scholarly journals Expression of OCT-4 and SOX-2 in Bone Marrow-Derived Human Mesenchymal Stem Cells during Osteogenic Differentiation

2016 ◽  
Vol 4 (1) ◽  
pp. 9-16 ◽  
Author(s):  
Igor Matic ◽  
Maja Antunovic ◽  
Sime Brkic ◽  
Pavle Josipovic ◽  
Katarina Caput Mihalic ◽  
...  
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Mesenchymal Stem Cells ◽  
Osteogenic Differentiation ◽  
Messenger Rna ◽  
Mrna Level ◽  
Human Mesenchymal Stem Cells ◽  
Human Mscs ◽  
Cell Nuclei

AIM: Determine the levels of expression of pluripotency genes OCT-4 and SOX-2 before and after osteogenic differentiation of human mesenchymal stem cells (hMSCs).METHODS: Human MSCs were derived from the bone marrow and differentiated into osteoblasts. The analyses were performed on days 0 and 14 of the cell culture. In vitro differentiation was evaluated due to bone markers – alkaline phosphatase (AP) activity and the messenger RNA (mRNA) expression of AP and bone sialoprotein (BSP). The OCT-4 and SOX-2 expression was evaluated at mRNA level by real-time qPCR and at protein level by immunocytochemistry.RESULTS: In vitro cultures on day 14 showed an increase in AP activity and upregulation of AP and BSP gene expression. OCT-4 and SOX-2 in undifferentiated hMSCs on day 0 is detectable and very low compared to tumor cell lines as a positive control. Immunocytochemistry detected OCT-4 in the cell nuclei prior (day 0) and post differentiation (day 14). On the same time points, cultures were negative for SOX-2 protein.CONCLUSION: Messenger RNA for pluripotency markers OCT-4 and SOX-2 isolated from hMSCs was less present, while OCT-4 protein was detected in cell nuclei prior and post differentiation into osteoblast lineage.

scholarly journals 22(R)-hydroxycholesterol for dopaminergic neuronal specification of MSCs and amelioration of Parkinsonian symptoms in rats

2021 ◽  
Vol 7 (1) ◽  
Author(s):  
Manisha Singh ◽  
Manish Jain ◽  
Samrat Bose ◽  
Ashutosh Halder ◽  
Tapas Chandra Nag ◽  
...  
Keyword(s):  
Parkinson’S Disease ◽  
Stem Cells ◽  
Parkinson's Disease ◽  
Dental Pulp ◽  
Neuronal Cells ◽  
Human Mesenchymal Stem Cells ◽  
Wistar Rat ◽  
Human Mscs ◽  
In Vitro Differentiation

AbstractOxysterols play vital roles in the human body, ranging from cell cycle regulation and progression to dopaminergic neurogenesis. While naïve human mesenchymal stem cells (hMSCs) have been explored to have neurogenic effect, there is still a grey area to explore their regenerative potential after in vitro differentiation. Hence, in the current study, we have investigated the neurogenic effect of 22(R)-hydroxycholesterol (22-HC) on hMSCs obtained from bone marrow, adipose tissue and dental pulp. Morphological and morphometric analysis revealed physical differentiation of stem cells into neuronal cells. Detailed characterization of differentiated cells affirmed generation of neuronal cells in culture. The percentage of generation of non-DA cells in the culture confirmed selective neurogenic potential of 22-HC. We substantiated the efficacy of these cells in neuro-regeneration by transplanting them into Parkinson’s disease Wistar rat model. MSCs from dental pulp had maximal regenerative effect (with 80.20 ± 1.5% in vitro differentiation efficiency) upon transplantation, as shown by various behavioural examinations and immunohistochemical tests. Subsequential analysis revealed that 22-HC yields a higher percentage of functional DA neurons and has differential effect on various tissue-specific primary human MSCs. 22-HC may be used for treating Parkinson’s disease in future with stem cells.

scholarly journals 3D-printed gelatin methacrylate (GelMA)/silanated silica scaffold assisted by two-stage cooling system for hard tissue regeneration

2021 ◽  
Vol 8 (2) ◽  
Author(s):  
Eunjeong Choi ◽  
Dongyun Kim ◽  
Donggu Kang ◽  
Gi Hoon Yang ◽  
Bongsu Jung ◽  
...  
Keyword(s):  
Osteogenic Differentiation ◽  
Tissue Regeneration ◽  
Cooling System ◽  
Composite Scaffold ◽  
Human Mesenchymal Stem Cells ◽  
Temperature Control System ◽  
Process Conditions ◽  
Hard Tissue ◽  
Two Stage ◽  
3D Printed

Abstract Among many biomaterials, gelatin methacrylate (GelMA), a photocurable protein, has been widely used in 3D bioprinting process owing to its excellent cellular responses, biocompatibility and biodegradability. However, GelMA still shows a low processability due to the severe temperature dependence of viscosity. To overcome this obstacle, we propose a two-stage temperature control system to effectively control the viscosity of GelMA. To optimize the process conditions, we evaluated the temperature of the cooling system (jacket and stage). Using the established system, three GelMA scaffolds were fabricated in which different concentrations (0, 3 and 10 wt%) of silanated silica particles were embedded. To evaluate the performances of the prepared scaffolds suitable for hard tissue regeneration, we analyzed the physical (viscoelasticity, surface roughness, compressive modulus and wettability) and biological (human mesenchymal stem cells growth, western blotting and osteogenic differentiation) properties. Consequently, the composite scaffold with greater silica contents (10 wt%) showed enhanced physical and biological performances including mechanical strength, cell initial attachment, cell proliferation and osteogenic differentiation compared with those of the controls. Our results indicate that the GelMA/silanated silica composite scaffold can be potentially used for hard tissue regeneration.

3D Printed PLA/PCL/TiO2 Composite for Bone Replacement and Grafting

MRS Advances ◽  
2018 ◽  
Vol 3 (40) ◽  
pp. 2373-2378 ◽  
Author(s):  
Sandra E. Nájera ◽  
Monica Michel ◽  
Nam-Soo Kim
Keyword(s):  
Biomedical Applications ◽  
Fused Deposition Modeling ◽  
Fracture Strain ◽  
Fused Deposition ◽  
In Vitro Biocompatibility ◽  
Bone Replacement ◽  
Deposition Modeling ◽  
3D Printed ◽  
The Stability

ABSTRACTPolymer composites of Polylactic acid (PLA) and poly-ε-caprolactone (PCL), containing small amounts of titanium oxide (TiO2) were developed for biomedical applications. These composite materials were prepared, and then printed using Fused Deposition Modeling (FDM). 3D printed structures were characterized to determine their mechanical properties and biocompatibility. DSC analysis yielded useful information regarding the immiscibility of the different polymers, and it was observed that the particles of TiO2 improved the stability of the polymers. The ultimate tensile strength and the fracture strain increased by adding TiO2 as a filler, resulting in values of approximately 45 MPa and 5.5 % elongation. The printed composites show excellent in vitro biocompatibility including cell proliferation and adhesion, and are therefore promising candidates to be used in the biomedical field for bone replacement procedures, due to their properties similar to those of cancellous bone.

Sign in / Sign up

Export Citation Format

Share Document