scholarly journals Silk Fibroin/Collagen/Chitosan Scaffolds Cross-Linked by a Glyoxal Solution as Biomaterials toward Bone Tissue Regeneration

Materials ◽  
2020 ◽  
Vol 13 (15) ◽  
pp. 3433
Author(s):  
Sylwia Grabska-Zielińska ◽  
Alina Sionkowska ◽  
Catarina C. Coelho ◽  
Fernando J. Monteiro
Keyword(s):  
Mechanical Properties ◽  
Silk Fibroin ◽  
Tissue Regeneration ◽  
Three Dimensional ◽  
Bone Tissue Regeneration ◽  
New Materials ◽  
Swelling Degree ◽  
Chitosan Scaffolds ◽  
Measured Density ◽  
Scanning Electron

In this study, three-dimensional materials based on blends of silk fibroin (SF), collagen (Coll), and chitosan (CTS) cross-linked by glyoxal solution were prepared and the properties of the new materials were studied. The structure of the composites and the interactions between scaffold components were studied using FTIR spectroscopy. The microstructure was observed using a scanning electron microscope. The following properties of the materials were measured: density and porosity, moisture content, and swelling degree. Mechanical properties of the 3D materials under compression were studied. Additionally, the metabolic activity of MG-63 osteoblast-like cells on materials was examined. It was found that the materials were characterized by a high swelling degree (up to 3000% after 1 h of immersion) and good porosity (in the range of 80–90%), which can be suitable for tissue engineering applications. None of the materials showed cytotoxicity toward MG-63 cells.

scholarly journals Fabrication of High-Strength and Porous Hybrid Scaffolds Based on Nano-Hydroxyapatite and Human-Like Collagen for Bone Tissue Regeneration

Polymers ◽  
2020 ◽  
Vol 12 (1) ◽  
pp. 61 ◽  
Author(s):  
Yannan Liu ◽  
Juan Gu ◽  
Daidi Fan
Keyword(s):  
Mechanical Properties ◽  
Enzymatic Hydrolysis ◽  
Bone Tissue ◽  
Tissue Regeneration ◽  
Bone Repair ◽  
Three Dimensional ◽  
High Strength ◽  
Biological Properties ◽  
Bone Tissue Regeneration

A novel, three-dimensional, porous, human-like collagen (HLC)/nano-hydroxyapatite (n-HA) scaffold cross-linked by 1,2,7,8-diepoxyoctane (DEO) was successfully fabricated, which showed excellent mechanical and superior biological properties for bone tissue regeneration in this study. The physicochemical characterizations of different n-HA/HLC/DEO (nHD) scaffolds were investigated by determining the morphology, compression stress, elastic modulus, Young’s modulus and enzymatic hydrolysis behavior in vitro. The results demonstrated that nHD-2 and nHD-3 scaffolds showed superior mechanical properties and resistance to enzymatic hydrolysis compared to nHD-1 scaffolds. The cell viability, live cell staining and cell adhesion analysis results demonstrated that nHD-2 scaffolds exhibited low cytotoxicity and excellent cytocompatibility compared with nHD-1 and nHD-3 scaffolds. Furthermore, subcutaneous injections of nHD-2 scaffolds in rabbits produced superior anti-biodegradation effects and histocompatibility compared with injections of nHD-1 and nHD-3 scaffolds after 1, 2 and 4 weeks. In addition, the repair of bone defects in rabbits demonstrated that nHD-2 scaffolds presented an improved ability for guided bone regeneration and reconstruction compared to commercially available bone scaffold composite hydroxyapatite/collagen (HC). Collectively, the results show that nHD-2 scaffolds show promise for application in bone tissue engineering due to their excellent mechanical properties, anti-biodegradation, anti-biodegradation, biocompatibility and bone repair effects.

scholarly journals Composite Scaffolds Containing Silk Fibroin, Gelatin, and Hydroxyapatite for Bone Tissue Regeneration and 3D Cell Culturing

Acta Naturae ◽  
2014 ◽  
Vol 6 (1) ◽  
pp. 96-101 ◽  
Author(s):  
M. M. Moisenovich ◽  
A. Yu. Arkhipova ◽  
A. A. Orlova ◽  
M. S. Drutskaya ◽  
S. V. Volkova ◽  
...  
Keyword(s):  
Bone Tissue ◽  
Silk Fibroin ◽  
Tissue Regeneration ◽  
Three Dimensional ◽  
Experimental Models ◽  
Bone Tissue Regeneration ◽  
Artificial Organ ◽  
Composite Scaffolds ◽  
Complex Processes ◽  
Healing Bone

Three-dimensional (3D) silk fibroin scaffolds were modified with one of the major bone tissue derivatives (nano-hydroxyapatite) and/or a collagen derivative (gelatin). Adhesion and proliferation of mouse embryonic fibroblasts (MEF) within the scaffold were increased after modification with either nano-hydroxyapatite or gelatin. However, a significant increase in MEF adhesion and proliferation was observed when both additives were introduced into the scaffold. Such modified composite scaffolds provide a new and better platform to study wound healing, bone and other tissue regeneration, as well as artificial organ bioengineering. This system can further be applied to establish experimental models to study cell-substrate interactions, cell migration and other complex processes, which may be difficult to address using the conventional two-dimensional culture systems.

scholarly journals Is Dialdehyde Chitosan a Good Substance to Modify Physicochemical Properties of Biopolymeric Materials?

2021 ◽  
Vol 22 (7) ◽  
pp. 3391
Author(s):  
Sylwia Grabska-Zielińska ◽  
Alina Sionkowska ◽  
Ewa Olewnik-Kruszkowska ◽  
Katarzyna Reczyńska ◽  
Elżbieta Pamuła
Keyword(s):  
Mechanical Properties ◽  
Tissue Engineering ◽  
Physicochemical Properties ◽  
Silk Fibroin ◽  
Three Dimensional ◽  
Microscopy Imaging ◽  
Maximum Deformation ◽  
One Step ◽  
Chitosan Blends ◽  
Fourier Transfer Infrared Spectroscopy

The aim of this work was to compare physicochemical properties of three dimensional scaffolds based on silk fibroin, collagen and chitosan blends, cross-linked with dialdehyde starch (DAS) and dialdehyde chitosan (DAC). DAS was commercially available, while DAC was obtained by one-step synthesis. Structure and physicochemical properties of the materials were characterized using Fourier transfer infrared spectroscopy with attenuated total reflectance device (FTIR-ATR), swelling behavior and water content measurements, porosity and density observations, scanning electron microscopy imaging (SEM), mechanical properties evaluation and thermogravimetric analysis. Metabolic activity with AlamarBlue assay and live/dead fluorescence staining were performed to evaluate the cytocompatibility of the obtained materials with MG-63 osteoblast-like cells. The results showed that the properties of the scaffolds based on silk fibroin, collagen and chitosan can be modified by chemical cross-linking with DAS and DAC. It was found that DAS and DAC have different influence on the properties of biopolymeric scaffolds. Materials cross-linked with DAS were characterized by higher swelling ability (~4000% for DAS cross-linked materials; ~2500% for DAC cross-linked materials), they had lower density (Coll/CTS/30SF scaffold cross-linked with DAS: 21.8 ± 2.4 g/cm3; cross-linked with DAC: 14.6 ± 0.7 g/cm3) and lower mechanical properties (maximum deformation for DAC cross-linked scaffolds was about 69%; for DAS cross-linked scaffolds it was in the range of 12.67 ± 1.51% and 19.83 ± 1.30%) in comparison to materials cross-linked with DAC. Additionally, scaffolds cross-linked with DAS exhibited higher biocompatibility than those cross-linked with DAC. However, the obtained results showed that both types of scaffolds can provide the support required in regenerative medicine and tissue engineering. The scaffolds presented in the present work can be potentially used in bone tissue engineering to facilitate healing of small bone defects.

Biocompatibility of plasma-treated poly(3-hydroxybutyrate-co-3-hydroxyvalerate) nanofiber mats modified by silk fibroin for bone tissue regeneration

2016 ◽  
Vol 68 ◽  
pp. 842-850 ◽  
Author(s):  
Irem Unalan ◽  
Oylum Colpankan ◽  
Aylin Ziylan Albayrak ◽  
Cansu Gorgun ◽  
Aylin Sendemir Urkmez
Keyword(s):  
Bone Tissue ◽  
Silk Fibroin ◽  
Tissue Regeneration ◽  
Bone Tissue Regeneration ◽  
Nanofiber Mats

Silk fibroin membrane used for guided bone tissue regeneration

2017 ◽  
Vol 70 ◽  
pp. 148-154 ◽  
Author(s):  
Yurong Cai ◽  
Junmao Guo ◽  
Cen Chen ◽  
Chenxue Yao ◽  
Sung-Min Chung ◽  
...  
Keyword(s):  
Bone Tissue ◽  
Silk Fibroin ◽  
Tissue Regeneration ◽  
Bone Tissue Regeneration

A highly bioactive and biodegradable poly(glycerol sebacate)–silica glass hybrid elastomer with tailored mechanical properties for bone tissue regeneration

2015 ◽  
Vol 3 (16) ◽  
pp. 3222-3233 ◽  
Author(s):  
Xin Zhao ◽  
Yaobin Wu ◽  
Yuzhang Du ◽  
Xiaofeng Chen ◽  
Bo Lei ◽  
...  
Keyword(s):  
Mechanical Properties ◽  
Bioactive Glass ◽  
Bone Tissue ◽  
Tissue Regeneration ◽  
Silica Glass ◽  
Bone Tissue Regeneration ◽  
Biodegradable Poly

A highly bioactive and biodegradable PGS–Silica bioactive glass hybrid elastomer with tailored mechanical properties was developed for bone tissue regeneration application.

scholarly journals Bone Regeneration in Critical-Sized Bone Defects Treated with Additively Manufactured Porous Metallic Biomaterials: The Effects of Inelastic Mechanical Properties

Materials ◽  
2020 ◽  
Vol 13 (8) ◽  
pp. 1992
Author(s):  
Marianne Koolen ◽  
Saber Amin Yavari ◽  
Karel Lietaert ◽  
Ruben Wauthle ◽  
Amir A. Zadpoor ◽  
...  
Keyword(s):  
Mechanical Properties ◽  
Bone Regeneration ◽  
Bone Tissue ◽  
Tissue Regeneration ◽  
Bulk Material ◽  
Bone Tissue Regeneration ◽  
Bony Tissue ◽  
Metallic Biomaterials ◽  
Cp Ti ◽  
Porous Biomaterials

Additively manufactured (AM) porous metallic biomaterials, in general, and AM porous titanium, in particular, have recently emerged as promising candidates for bone substitution. The porous design of such materials allows for mimicking the elastic mechanical properties of native bone tissue and showed to be effective in improving bone regeneration. It is, however, not clear what role the other mechanical properties of the bulk material such as ductility play in the performance of such biomaterials. In this study, we compared the bone tissue regeneration performance of AM porous biomaterials made from the commonly used titanium alloy Ti6Al4V-ELI with that of commercially pure titanium (CP-Ti). CP-Ti was selected because of its high ductility as compared to Ti6Al4V-ELI. Critical-sized (6 mm diameter) femoral defects in rats were treated with implants made from both Ti6Al4V-ELI and CP-Ti. Bone regeneration was assessed up to 11 weeks using micro-CT scanning. The regenerated bone volume was assessed ex vivo followed by histology and biomechanical testing to assess osseointegration of the implants. The bony defects treated with AM CP-Ti implants generally showed higher volumes of regenerated bone as compared to those treated with AM Ti6Al4V-ELI. The torsional strength of the two titanium groups were similar however, and both considerably lower than those measured for intact bony tissue. These findings show the importance of material type and ductility of the bulk material in the ability for bone tissue regeneration of AM porous biomaterials.

scholarly journals Alginate/Gelatin Hydrogels Reinforced with TiO2 and β-TCP Fabricated by Microextrusion-based Printing for Tissue Regeneration

Polymers ◽  
2019 ◽  
Vol 11 (3) ◽  
pp. 457 ◽  
Author(s):  
Rodrigo Urruela-Barrios ◽  
Erick Ramírez-Cedillo ◽  
A. Díaz de León ◽  
Alejandro Alvarez ◽  
Wendy Ortega-Lara
Keyword(s):  
Mechanical Properties ◽  
Tissue Engineering ◽  
Tissue Regeneration ◽  
Average Pore Size ◽  
Three Dimensional ◽  
Engineering Applications ◽  
Average Pore ◽  
Viscous Deformation ◽  
Freeze Dried ◽  
3D Printed

Three-dimensional (3D) printing technologies have become an attractive manufacturing process to fabricate scaffolds in tissue engineering. Recent research has focused on the fabrication of alginate complex shaped structures that closely mimic biological organs or tissues. Alginates can be effectively manufactured into porous three-dimensional networks for tissue engineering applications. However, the structure, mechanical properties, and shape fidelity of 3D-printed alginate hydrogels used for preparing tissue-engineered scaffolds is difficult to control. In this work, the use of alginate/gelatin hydrogels reinforced with TiO2 and β-tricalcium phosphate was studied to tailor the mechanical properties of 3D-printed hydrogels. The hydrogels reinforced with TiO2 and β-TCP showed enhanced mechanical properties up to 20 MPa of elastic modulus. Furthermore, the pores of the crosslinked printed structures were measured with an average pore size of 200 μm. Additionally, it was found that as more layers of the design were printed, there was an increase of the line width of the bottom layers due to its viscous deformation. Shrinkage of the design when the hydrogel is crosslinked and freeze dried was also measured and found to be up to 27% from the printed design. Overall, the proposed approach enabled fabrication of 3D-printed alginate scaffolds with adequate physical properties for tissue engineering applications.

Three-dimensional bioglass-collagen-phosphatidylserine scaffolds designed with functionally graded structure and mechanical features

2018 ◽  
Vol 63 (3) ◽  
pp. 255-259 ◽  
Author(s):  
Chunrong Yang ◽  
Huazhong Wu ◽  
Shou Chen ◽  
Guangyu Kang
Keyword(s):  
Mechanical Properties ◽  
Drug Release ◽  
Bone Defect ◽  
Release Kinetics ◽  
Three Dimensional ◽  
Bottom Layer ◽  
Functionally Graded ◽  
Bone Tissue Regeneration ◽  
Defect Sites ◽  
Form Stability

Abstract The development of scaffolds featuring spatiotemporal controlled release of drugs is highly desirable. The goal of this study is to construct an inhomogeneous scaffold with gradient pore structure from top layer to bottom layer. The scaffolds were prepared using bioglass (BG), phosphatidylserine (PS) and steroidal saponins (SS) loaded collagen (COL) microparticles as the main components. The resulting scaffold constructs were characterized in terms of their morphology, drug release kinetics and mechanisms, as well as macroscopic form stability and mechanical properties. Pore interconnectivity and graded distribution were demonstrated using scanning electron microscopy (SEM). Such constructs have been further shown to be advantageous for temporal and spatial control of drug release and deposition in the scaffolds, with a potential to repair bone defect more precisely and effectively. Changes in the BG content resulted in distinct macroscopic form stability and mechanical properties to scaffolds. An increase in the BG content in scaffolds led to less volume swell as well as higher ultimate strength and compressive modulus, which makes the scaffolds mechanically adjustable according to certain structures and properties of different bone defect sites. The developed scaffolds may show promise for promoting bone tissue regeneration.

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