Sustainable Rabbit Skin Glue to Produce Bioactive Nanofibers for Nonactive Wound Dressings

This paper assessed the collagen glue (Col) from rabbit skin for use as a raw material in combination with different water-based dispersants of antimicrobial agents such as ZnO NPs, TiO2 NPs doped with nitrogen and Ag NPs (TiO2-N-Ag NPs), and chitosan (CS) for the production of biocompatible and antimicrobial nanofibers. The electrospun nanofibers were investigated by scanning electron microscopy (SEM), attenuated total reflectance in conjunction with Fourier-transform infrared spectroscopy (ATR-FT-IR) analyses and antioxidant activity. The biocompatibility of electrospun nanofibers was investigated on cell lines of mouse fibroblast NCTC (clone L929) using MTT test assays. Antimicrobial activity was performed against Escherichia coli and Staphylococcus aureus bacteria and Candida albicans pathogenic fungus. Electrospun antimicrobial nanofibers based on collagen glue achieved reduction in the number of viable microorganisms against both fungi and bacteria and exhibited multiple inhibitory actions of fungal and bacterial strains. The electrospun nanofibers showed average dimension sizes in the range of 30–160 nm. The results indicated that both Col/TiO2-N-Ag NPs and Col/CS formulations are suitable for cell proliferation and may be useful for producing of nonactive wound dressings.

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Biosynthesis of Triangular-Shape ZnO Nanoparticles Using Tecoma Stans and Its Antimicrobial Activity

10.26434/chemrxiv.13337072.v1 ◽

2020 ◽

Author(s):

Samuel Lalthazuala Rokhum ◽

Aayushi Biswas ◽

Bishwajit Changmai ◽

Vanlalveni Chhangte ◽

R. Lalfakzuala ◽

...

Keyword(s):

Electron Microscopy ◽

Antimicrobial Agents ◽

Ultra Violet ◽

Achromobacter Xylosoxidans ◽

Bacterial Strains ◽

Zno Nps ◽

Blue Band ◽

Triangular Shape ◽

Zno Crystal ◽

Photoluminescence Studies

<div>The present work reports the first green synthesis of zinc oxide nanoparticles (ZnO-NPs) using Tecoma stans leaf extract. The ZnO-NPs have been investigated by X-Ray Diffraction (XRD), Ultra Violet-Visible (UV-Vis), Scanning Electron Microscopy (SEM), Transmission Electron Microscopy (TEM) and Fourier Transform-Infra Red (FT-IR) analysis. XRD investigation confirms the crystalline structure of ZnO. The TEM images show triangular shape ZnO-NPs with sizes running from 15-20 nm. The XPS spectrum revealed the presence of Zn and O in the sample. Photoluminescence studies of ZnO-NPs displayed a sharp emission of blue band at 447 nm which is attributed to the defect structures in ZnO crystal. The presence of alcoholic, phenolic amide groups in the plant extracts is responsible for the formation of ZnO-NPs. The synthesized ZnO-NPs showed a very high antibacterial property against five bacterial strains such as Bacillus cereus,</div><div>Acinetobacter johnsonii, Achromobacter xylosoxidans, Achromobacter spanius and Chromobacterium pseudoviolaceum, with the highest zone of inhibition (ZOI) of 24 mm being shown against Achromobacter spanius strain. Further, the synthesized nanoparticles displayed excellent activities against four fungal strains, where a highest ZOI of 30 mm was observed against Penicillium citirinum, hence proving its high efficacy as antimicrobial agents.</div>

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Electrospun Antibacterial Nanomaterials for Wound Dressings Applications

Membranes ◽

10.3390/membranes11120908 ◽

2021 ◽

Vol 11 (12) ◽

pp. 908

Author(s):

Aysegul Gul ◽

Izabela Gallus ◽

Akshat Tegginamath ◽

Jiri Maryska ◽

Fatma Yalcinkaya

Keyword(s):

Wound Healing ◽

Wound Dressing ◽

Bacterial Infections ◽

Antimicrobial Agents ◽

Chronic Wounds ◽

Antibacterial Properties ◽

Electrospun Nanofibers ◽

Electrospinning Process ◽

Wound Dressings ◽

Promising Solution

Chronic wounds are caused by bacterial infections and create major healthcare discomforts; to overcome this issue, wound dressings with antibacterial properties are to be utilized. The requirements of antibacterial wound dressings cannot be fulfilled by traditional wound dressing materials. Hence, to improve and accelerate the process of wound healing, an antibacterial wound dressing is to be designed. Electrospun nanofibers offer a promising solution to the management of wound healing, and numerous options are available to load antibacterial compounds onto the nanofiber webs. This review gives us an overview of some recent advances of electrospun antibacterial nanomaterials used in wound dressings. First, we provide a brief overview of the electrospinning process of nanofibers in wound healing and later discuss electrospun fibers that have incorporated various antimicrobial agents to be used in wound dressings. In addition, we highlight the latest research and patents related to electrospun nanofibers in wound dressing. This review also aims to concentrate on the importance of nanofibers for wound dressing applications and discuss functionalized antibacterial nanofibers in wound dressing.

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Antimicrobial efficacy of silver, zinc oxide, and titanium dioxide nanoparticles incorporated in orthodontic bonding agent

Journal of Baghdad College of Dentistry ◽

10.26477/jbcd.v31i3.2693 ◽

2019 ◽

Vol 31 (3) ◽

pp. 10-16 ◽

Cited By ~ 1

Author(s):

Sarah Y Hailan ◽

Mustafa M. Al-Khatieeb

Keyword(s):

Antimicrobial Agents ◽

Titanium Dioxide Nanoparticles ◽

Antibacterial Properties ◽

Mutans Streptococci ◽

Bonding Agent ◽

Ag Nps ◽

Zno Nps ◽

White Spots ◽

Orthodontic Bonding ◽

Group 10

Background: One of the most important complications of fixed orthodontic treatment is formation of white spots, which are initial carious lesions. Addition of antimicrobial agents into orthodontic adhesive material might be wise solution for prevention of white spots formation. The aim of this study was to evaluate the antibacterial properties of orthodontic adhesive primer against S. Mutans after adding the three different types of nanoparticles (Ag, ZnO, or TiO2). Materials and methods: Discs were prepared using empty insulin syringe approximately 2 mm×2 mm rounded in shape specimens (40 discs) were divided into four groups (ten discs for each group): The first group was the control (made from primer only), the second group (10 discs made from primer and incorporation of Ag NPs), the third group (10 discs made from primer and incorporation of ZnO NPs), and the fourth group (10 discs made from primer and incorporation of TiO2 NPs). The antibacterial properties of orthodontic bonding agent after incorporation of (Ag, ZnO, or TiO2) nanoparticles were evaluated by disc diffusion test and viable count of mutans streptococci. Results: The results of this study showed that there were high significant differences between the all groups using ANOVA F-test, and the colony forming unit were 99×10³, 39.6×10³, 19.4×10³, 6.6×10³ CFU/mL respectively. Conclusion: The incorporation of these nanoparticles (Ag, ZnO, or TiO2) into Transbond ™ XT adhesive primer helps to enhance the antibacterial properties of primer against the S. mutans.

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Silver Nanoparticles Synthesized through Green Methods Using Escherichia coli Top 10 (Ec-Ts) Growth Culture Medium Exhibit Antimicrobial Properties against Nongrowing Bacterial Strains

Journal of Nanomaterials ◽

10.1155/2019/4637325 ◽

2019 ◽

Vol 2019 ◽

pp. 1-8 ◽

Cited By ~ 7

Author(s):

Esther Baltazar-Encarnación ◽

Carlos E. Escárcega-González ◽

Ximena G. Vasto-Anzaldo ◽

María Elena Cantú-Cárdenas ◽

J. Rubén Morones-Ramírez

Keyword(s):

Silver Nanoparticles ◽

Metal Nanoparticles ◽

Antimicrobial Agents ◽

Culture Media ◽

Size Distributions ◽

Bacterial Strains ◽

Ag Nps ◽

E Coli ◽

Low Concentrations ◽

Metabolic States

Finding novel antibiotics and antimicrobial materials has become of great importance to modern society due to the alarming increase in the development of multidrug resistance in various bacterial strains. This problem is even more complex when infections involve bacterial strains in stationary metabolic states, since most of the antibiotics found in the market do not have an effect on bacteria in dormant metabolic states. A promising field to aid in the solution of this problem is nanotechnology, since it offers a wide avenue for the development of potential therapeutics, specifically the use of silver metal nanoparticles. Silver nanoparticles have proven to be highly effective antimicrobial agents and excellent candidates to be engineered and designed into clever delivery systems, taking advantage of their rapid and potent toxicity on prokaryotic cells at low concentrations. Metal nanoparticles are most commonly synthetized through one or a series of redox chemical reactions using powerful but environmentally toxic-reducing agents. Therefore, in this work, we propose a biosynthesis method that allows the production of nanoparticles, with homogenous shapes and narrow size distributions, through an environmentally friendly technique that does not produce toxic residues. Here, silver nanoparticles were produced from silver salt (AgNO3) using three different growth culture media residues from E. coli top 10. The three different culture media residues used included LB, LBN, and LBE; all of them displaying a different chemical and nutrient composition. Here, after characterization of the different silver nanoparticles produced with the different media, we demonstrated that the LB culture-conditioned media was the most suitable to produce them since they displayed the most narrow size distribution, with an average 10.6 nm in diameter, a relatively low standard deviation of 5.5 nm, and a narrow UV-vis spectrum absorption peak at 420 nm. The other methods presented larger nanoparticle sizes and broader size distributions. Furthermore, nanoparticles produced with LB Lennox were found to be, at very low concentrations, effective antimicrobial agent against E. coli top 10 at stationary phase. Therefore, these results seem to contribute knowledge linked to the production of antimicrobial nanoparticles (Ag-NPs) through green synthesis and represent a platform to treat infections caused by nongrowing bacteria.

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Effect of Ag, Cu, and ZnO Nanoparticle Suspensions on the Antimicrobial Activity of Tribulus Terrestris Herbal Extracts

Journal of Nano Research ◽

10.4028/www.scientific.net/jnanor.45.95 ◽

2017 ◽

Vol 45 ◽

pp. 95-109 ◽

Cited By ~ 2

Author(s):

Marzough A. Albalawi ◽

Jayda Eldiasty ◽

Syed Khasim ◽

Nacer Badi

Keyword(s):

Antimicrobial Activity ◽

Tribulus Terrestris ◽

Moderate Activity ◽

Zno Nanoparticle ◽

Bacterial Strains ◽

Ag Nps ◽

Zno Nps ◽

Medicinal Plant Extracts ◽

Nanoparticle Suspensions ◽

Nanoparticles Suspension

In this study, the antimicrobial growth inhibition and mechanistic activities of silver (Ag NPs), zinc oxide (ZnO NPs), and copper (Cu NPs) nanoparticles were investigated in presence of medicinal plant extracts of TribulusTerrestris. The extract in different concentrations (5, 10, 20, and 30%) along with nanoparticles suspension mixture was used for antimicrobial activity testing against some human pathogenic microorganisms. Among the bacterial strains tested, Escherichia coli and Proteus sp were most susceptibile at 30% concentration followed by moderate activity against Morganellasp, EntrococcusFaecalis, Staphylococcus aureus, and Candida albicans.TribulusTerrestris had no effect on Acetobacterspand Streptococcus agalactia. The nanoparticles used in the present investigation have shown excellent antimicrobial activity in the presence of TribulusTerrestris, hence they can be used as potent sources for antibacterial agents.

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Nanoparticle Synthesis from Biowaste and Its Potential as an Antimicrobial Agent

10.21203/rs.3.rs-718715/v1 ◽

2021 ◽

Author(s):

Rishana Saffar ◽

Athira PV ◽

Kangkana Kalita ◽

Suba G A Manuel ◽

Pradeep N

Keyword(s):

Antimicrobial Agents ◽

Antimicrobial Properties ◽

Nanoparticle Synthesis ◽

Punica Granatum ◽

Diffusion Method ◽

Potential Candidate ◽

Zone Of Inhibition ◽

Ag Nps ◽

Zno Nps ◽

X Ray

Abstract Biosynthesized nanoparticles are gaining more importance because of their unique biological applications and can be synthesised using biowastes like fruit peels that acts as a better alternative against conventional physical and chemical methods. This work reports an ecofriendly, reliable and cost-effective synthesis of silver and zinc oxide nanoparticles (Ag and ZnO NPs) chemically and biologically using fresh peel extracts of Punica granatum and Musa acuminata. The synthesised nanoparticles were characterized using UV-Visible spectroscopy, X-ray Diffraction (XRD), Scanning electron microscopy (SEM) and Energy dispersive X-ray analysis (EDS). The size of chemically and biosynthesized Ag and ZnO NPs ranged around 12-20 nm and 0.4-24 nm respectively. Both biosynthesized Ag and ZnO NPs were found to be nanospheres whereas chemically synthesised ZnO NPs were nanoflakes and showed maximum absorbance in the range of 250-550nm. Antimicrobial properties of the synthesized nanoparticles were assessed by well diffusion method against Gram negative Escherichia coli and Gram-positive Bacillus subtilis. Biosynthesized Ag NPs were found to be more efficient against E. coli as compared to ZnO NPs wherein biosynthesized M. acuminata Ag NPs exhibiting maximum zone of inhibition (28.4±14.66mm). Similarly, biosynthesized ZnO NPs were more efficient against B. subtilis as compared to biosynthesized Ag NPs wherein biosynthesized P. granatum ZnO NPs exhibiting maximum zone of inhibition (14.08±3.03mm). Hence biosynthesized NPs can be exploited as a potential candidate for antimicrobial agents. Scientific and systematic use of biowaste for NPs synthesis could in turn result in a sustainable solution for waste management.

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Sustainable Coated Nanostructures Based on Alginate and Electrospun Collagen Loaded with Antimicrobial Agents

Coatings ◽

10.3390/coatings11020121 ◽

2021 ◽

Vol 11 (2) ◽

pp. 121

Author(s):

Ecaterina Matei ◽

Carmen Gaidau ◽

Maria Râpă ◽

Laura Mihaela Stefan ◽

Lia-Mara Ditu ◽

...

Keyword(s):

Escherichia Coli ◽

Staphylococcus Aureus ◽

Antimicrobial Agents ◽

Biological Properties ◽

Wound Dressings ◽

Bacterial Strains ◽

Force Microscopy ◽

Atomic Force ◽

Nanostructured Systems

In this study, sodium alginate film (Alg) was coated with electrospun collagen glue (Col) extracted from rabbit skin waste, loaded with different commercial antimicrobial agents (chitosan, AG425K and ZnONPs) and investigated in terms of morphological, structural and biological properties. The coated nanostructures were characterized using scanning electron microscopy coupled with the energy-dispersive X-ray (SEM/EDS), Attenuated Total Reflectance Fourier-Transform Infrared spectroscopy (ATR FT-IR), and Atomic Force Microscopy (AFM) tests. The cytotoxicity was investigated on murine L929 fibroblasts using 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide salt (MTT) and lactate dehydrogenase (LDH) assays. Microbiological tests were performed against Staphylococcus aureus ATCC 25923, Escherichia coli ATCC 25922, Pseudomonas aeruginosa ATCC 27853 and Candida albicans ATCC 27853 standard strains. In vitro cell culture tests showed a good cytocompatibility of the coated nanostructured systems, except the sample loaded with ZnONPs, which exhibited a highly cytotoxic effect. Alg-Col-ZnONPs nanostructure inhibited the growth and multiplication of the Staphylococcus aureus ATCC 25923 and Escherichia coli ATCC 25922 bacterial strains. The results of new coated nanostructures may be useful for the development of sustainable biomaterials in a circular economy, with bioactive properties for medical wound dressings.

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Implementation of Penicillium sp. as Raw Material for Synthesizing Metal Nanoparticles for Antibiosis

Materials Science Forum ◽

10.4028/www.scientific.net/msf.760.33 ◽

2013 ◽

Vol 760 ◽

pp. 33-38 ◽

Cited By ~ 4

Author(s):

B.K. Nayak ◽

M. Amin Bhat ◽

Anima Nanda

Keyword(s):

Silver Nanoparticles ◽

Metallic Nanoparticles ◽

Pathogenic Bacteria ◽

Antimicrobial Agents ◽

Raw Material ◽

Microbial Pathogens ◽

High Dose ◽

Ag Nps ◽

Tem Analysis ◽

Major Health Problem

Silver Nanoparticles (Ag-NPs) have been known to have inhibitory and bactericidal effects. Resistance to antimicrobial agents by pathogenic bacteria has emerged in recent years and is a major health problem. The ability of some microorganisms such as bacteria and fungi to control the synthesis of metallic Nanoparticles should be employed in the search for new materials. The present work is the study of biosynthesis of silver Nanoparticles synthesized from differentPenicilliumspp.ThePenicilliumspecies were identified by employing microscopic methods and laboratory manuals. The Nanoparticles synthesis was done by extracellular and intracellular methods. The silver Nanoparticles formed were characterized by Uv-vis spectrophotometer followed by TEM analysis. The maximum absorbance 410nm confirmed the formation of silver Nanoparticles and TEM revealed the particle size in the range of 30-40nm, which after confirmation was checked for its antibacterial activity against selected microbial pathogens likeBacillus cereus, Proteus vulgaris, Staphylococcus aureusandStaphylococcus epidermidis.The Nanoparticles synthesized byPenicilliumspecies showed a well anti microbial activity that was further compared with different antibiotics to check the Nanoparticles activity which can be substituted in place of high dose antibiotics.

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Synergistic Antibacterial Activity of Nanohybrid Materials ZnO–Ag and ZnO–Au: Synthesis, Characterization, and Comparative Analysis of Undoped and Doped ZnO Nanoparticles

Australian Journal of Chemistry ◽

10.1071/ch14123 ◽

2015 ◽

Vol 68 (2) ◽

pp. 288 ◽

Cited By ~ 10

Author(s):

Adriana Berenice Pérez Jiménez ◽

Carlos Alberto Huerta Aguilar ◽

Jorge Manuel Vázquez Ramos ◽

Pandiyan Thangarasu

Keyword(s):

Electron Microscopy ◽

Zno Nanoparticles ◽

Antibacterial Properties ◽

Growth Control ◽

Bacterial Cells ◽

Bacterial Strains ◽

Ag Nps ◽

Zno Nps ◽

Au Nps ◽

X Ray

ZnO nanoparticles (NPs) were prepared using the hydrothermal method, and then doped with Ag or Au NPs, yielding ZnO NPs, ZnO–Ag NPs, and ZnO–Au NPs, which were characterized by transmission electron microscopy, X-ray diffraction, and energy-dispersive X-ray spectroscopy. The synthesized nanomaterials were analyzed for their antibacterial properties against bacterial strains (Staphylococcus aureus, Bacillus cereus, Escherichia coli, and Salmonella typhi) by qualitative and quantitative assays. Minimal inhibitory concentration (MIC) results show that growth control is more effective for Gram-positive bacteria than for Gram-negative bacteria. Although ZnO NPs and Ag NPs are antibacterial agents, the lowest bacterial growth was observed for ZnO–Ag NPs, showing that the doped Ag NPs greatly facilitate the interaction between the microbial cells and the NP surface. Though the same antibacterial effect was expected for ZnO–Au NPs, the inhibition activity was very close to that of ZnO NPs. The order of bacterial cell growth inhibition was ZnO–Ag NPs >> ZnO–Au NPs ~ ZnO NPs >> ZnO powder. We also analyzed the morphology of bacterial cells treated with NPs by scanning electron microscopy.

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