scholarly journals Study of diversity of anthocyanin composition in bilberry (Vaccinium myrtillus L.) fruits

Medicina ◽  
2007 ◽  
Vol 43 (12) ◽  
pp. 971 ◽  
Author(s):  
Deividas Burdulis ◽  
Liudas Ivanauskas ◽  
Vidmantas Dirsė ◽  
Saulius Kazlauskas ◽  
Almantas Ražukas
Keyword(s):  
High Performance ◽  
Qualitative Evaluation ◽  
Vaccinium Myrtillus ◽  
Quantitative Composition ◽  
Anthocyanin Content ◽  
Total Anthocyanin ◽  
Total Anthocyanin Content ◽  
Total Anthocyanins ◽  
Hydrolysis Of ◽  
Vaccinium Myrtillus L

Qualitative and quantitative composition of anthocyanins in bilberry (Vaccinium myrtillus L.) fruits was assayed. The aim of our study was to evaluate total anthocyanin content and their composition in bilberries collected from various regions and at different time. For the quantification of total anthocyanins in frozen fruits, the spectrophotometrical assay was performed. The highest amount of anthocyanins in bilberry fruits, collected in Lithuania, was found in samples from Šilutė (0.399%), the lowest one – from Valkininkai region (0.264%), but higher amounts of anthocyanins were found in the samples collected in Russia (Archangelsk region) and Sweden (Stockholm region). High-performance liquid chromatography was applied for qualitative evaluation of individual anthocyanins in the different material. Quantification of anthocyanidin content was performed after acidic hydrolysis of anthocyanin glycosides. Chromatographic analysis has shown that there are no differences in qualitative composition of anthocyanidins. In all samples, cyanidin was found in the highest quantities (mean amount 0.053 µg/mL). Delphinidin and petunidin was found in quantities 2.5 fold lower than cyanidin, and malvidin and peonidin were found in the smallest quantities. Only in the blueberries collected in Sweden, malvidin was found in the highest amount. It was 1.5 fold higher than amounts of petunidin and delphinidin.

scholarly journals Analysis of anthocyanin content in bilberry (Vaccinium myrtillus L.) fruit crude drugs by high-performance liquid chromatography method

Medicina ◽  
2007 ◽  
Vol 43 (7) ◽  
pp. 568 ◽  
Author(s):  
Deividas Burdulis ◽  
Liudas Ivanauskas ◽  
Valdas Jakštas ◽  
Valdimaras Janulis
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
High Performance ◽  
Vaccinium Myrtillus ◽  
Quantitative Composition ◽  
Anthocyanin Content ◽  
Qualitative And Quantitative ◽  
Crude Drug ◽  
Crude Drugs ◽  
Vaccinium Myrtillus L

Bilberry (Vaccinium myrtillus L.) is rich in flavonoids (major part anthocyanins), tannins, phenolic and organic acids, and other biologically active compounds. Anthocianins possess a broad spectrum of therapeutic properties. Variation in anthocyanin content of bilberry fruit crude drugs is significant in optimizing collection conditions. Bilberry (Vaccinium myrtillus L.) crude drug samples were collected in July–September of 2006 in natural environment in the territory of Lithuania. Ultrasonic extraction was applied using methanol as solvent. Acidic hydrolysis was performed. Qualitative and quantitative composition of five major anthocyanidins (delphinidin, cyanidin, petunidin, peonidin, and malvidin) was estimated by high-performance liquid chromatography in frozen fruits. Cyanidin predominated in all crude drug samples. Dynamics of variation in qualitative and quantitative composition of anthocyanidins was estimated in bilberry fruits collected during vegetation period. The greatest total amount of all analyzed anthocyanidins was determined at the end of investigated period. Variation in qualitative and quantitative content of anthocyanidins in bilberry fruits collected in different regions of Lithuania was estimated as well. The greatest total amounts of anthocyanidins were found in samples collected in Krikštoniai forest (1.78%) and Prienai pinewood (2.13%) and the lowest amounts – in samples collected in Balkasodis forest (1.14%) and Ryliškės forest (0.99%). Significant variation in anthocyanidin content reaching 28.40% (delphinidin) was determined in bilberry fruits. It is important for the standardization of bilberry fruit crude drugs.

scholarly journals Fruit Anthocyanin Profile and Berry Color of Muscadine Grape Cultivars and Muscadinia Germplasm

HortScience ◽  
2013 ◽  
Vol 48 (10) ◽  
pp. 1235-1240 ◽  
Author(s):  
Patrick J. Conner ◽  
Dan MacLean
Keyword(s):  
High Performance ◽  
Color Space ◽  
Color Stability ◽  
Anthocyanin Content ◽  
Total Anthocyanin ◽  
Vitis Rotundifolia ◽  
Total Anthocyanins ◽  
Muscadine Grape ◽  
Germplasm Analysis ◽  
Promising Source

Anthocyanin content and composition and CIE 1976 (L*, a*, b*) color space (CIELAB) color coordinates were examined for the skin of 22 muscadine grape (Vitis rotundifolia Michx.) cultivars and Muscadinia Planch germplasm. Analysis of berry skin extracts by high-performance liquid chromatography (HPLC) determined that anthocyanin content varied from less than 100 μg·g−1 in bronze and pink berries to over 5500 μg·g−1 in highly pigmented black berries. The anthocyanins delphinidin, cyanidin, petunidin, pelargonidin, peonidin, and malvidin were detected in their 3,5-diglucosidic forms. Analysis of berry color with a colorimeter revealed chroma (C*) ranged from 2.4 to 22.8 with the highest values occurring in bronze- and red-colored berries. As anthocyanin concentration increased, lightness (L*) decreased to a low of 20 to 23 in black-colored berries. Pink and red skin colors were primarily a result of lower levels of total anthocyanins, although there was also a shift away from delphinidin and petunidin production toward more cyanidin and peonidin. Malvidin, the most important anthocyanin for muscadine wine and juice color stability, was only abundant in a few clones, all of which had V. munsoniana (Simpson ex Munson) Small or V. popenoei (Fennell) Small in their pedigree. The interspecific hybrid ‘Fennell’s 3-way Hybrid’ had the largest proportion of malvidin, contributing ≈58% of the total anthocyanin content. This clone also had low levels of delphinidin and high total levels of anthocyanin, making it a promising source for the improvement of muscadine grape pigment profiles.

scholarly journals Anthocyanin Pigment and Total Phenolic Content of Three Vaccinium Species Native to the Pacific Northwest of North America

HortScience ◽  
2004 ◽  
Vol 39 (5) ◽  
pp. 959-964 ◽  
Author(s):  
Jungmin Lee ◽  
Chad E. Finn ◽  
Ronald E. Wrolstad
Keyword(s):  
Pacific Northwest ◽  
Total Phenolic Content ◽  
High Performance ◽  
Phenolic Content ◽  
Total Phenolics ◽  
Total Phenolic ◽  
Anthocyanin Content ◽  
Anthocyanin Pigment ◽  
Total Anthocyanin ◽  
Total Anthocyanins

The total anthocyanin and total phenolic content of wild (samples from 4 populations) and cultivated (samples from 32 populations) Pacific Northwestern American Vaccinium species (V. membranaceum, V. ovalifolium, and V. deliciosum) were evaluated. The total monomeric anthocyanin content of all huckleberry samples analyzed ranged from 101 to 400 mg/100 g (expressed as cyanidin-3-glucoside), and the total phenolics varied from 367 to 1286 mg/100 g (expressed as gallic acid). Cluster analysis separated the samples into four different groups based on their anthocyanin and total phenolic content. Two groups had greater anthocyanin pigment and total phenolics; one consisted entirely of cultivated V. ovalifolium (LIG10, VAC485, VAC487, LIG33, LIG9, LIG2, and VAC349) and the other consisted of just cultivated V. membranaceum (LIG25). Significant variations in total anthocyanins, total phenolics, and the ratio of the total anthocyanins and total phenolics were observed among the different V. membranaceum, V. ovalifolium, and V. deliciosum populations cultivated in the Willamette Valley, Ore. The profile of the individual anthocyanins of the wild V. membranaceum, wild V. ovalifolium, and V. corymbosum `Rubel' were conducted by high-performance liquid chromatography. The chromatograms of V. membranaceum, V. ovalifolium, and `Rubel' were distinctly different in the amounts of delphinidin, cyanidin, and malvidin glycosides.

scholarly journals Detection of Copper (II) and Iron (III) in Aqueous Solutions Using the Spectroscopic Characteristics of Bugnay (Antidesma bunius) Anthocyanins

2014 ◽  
pp. 102-118
Author(s):  
Jonathan Barcelo ◽  
Jan Narlo Abril Abril ◽  
Khristine Mereille Castillo ◽  
Alloisa Diaz ◽  
Jonathan Paul Ladera ◽  
...  
Keyword(s):  
High Performance ◽  
Solid Phase ◽  
Copper Ions ◽  
Reversed Phase ◽  
Differential Method ◽  
Anthocyanin Content ◽  
Total Anthocyanin ◽  
Acidic Solutions ◽  
Total Anthocyanin Content ◽  
Spectroscopic Characteristics

The spectrophotometric characteristics of bugnay (Antidesma bunius) anthocyanins in acidified solutions of copper (Cu2+) and iron (Fe3+) were investigated after one hour of reaction to determine the changes in their absorbance characteristics. Anthocyanins from bugnay were isolated using solid phase extraction followed by evaporation at 40°C. The total anthocyanin content of the extract was determined to be 103.87 ± 2.91 mg/L cyanidin-3O-glucoside equivalents using pH differential method. Maximum absorbance readings at pH 1.0 and 4.5 were determined to be at 520 nm and 350 nm, respectively. Cyanidin-3-glucoside was identified as one of the components of the three pigments in the extract using reversed phase high performance liquid chromatography. At pH 1.0, copper caused greater hypochromic shift of bugnai anthocyanins compared to iron (p<0.01) while iron caused greater hypochromic shift at pH 4.5. Copper also caused hypsochromic shift of anthocyanins from 520nm to 350nm at pH 1.0 but not at pH 4.5. Correlation analysis showed a significant moderate positive correlation between mean % hypochromic shift and concentration of copper ions at pH 1.0 (R2 = 0.603, ρ<0.01) and 4.5 (R2 = 0.533, ρ<0.01), and iron at pH 4.5 (R2 = 0.638, ρ<0.01). The spectroscopic characteristics of bugnay anthocyanins at 350 nm and 520 nm can be used as parameters to detect copper and iron in acidic solutions.

scholarly journals Changes in Polyphenolics during Storage of Products Prepared with Freeze-Dried Wild Blueberry Powder

Foods ◽  
2020 ◽  
Vol 9 (4) ◽  
pp. 466 ◽  
Author(s):  
Laura Lavefve ◽  
Cindi Brownmiller ◽  
Luke Howard ◽  
Donovon Reeves ◽  
Sean H. Adams ◽  
...  
Keyword(s):  
Lower Amount ◽  
Anthocyanin Content ◽  
Total Anthocyanin ◽  
Freeze Dried ◽  
Total Anthocyanin Content ◽  
Powder Samples ◽  
Total Anthocyanins ◽  
Health Promoting ◽  
The Stability ◽  
Wild Blueberry

Wild blueberry (WBB) powder can be added to the formulation of foods to encourage consumption of health-promoting polyphenolics, but the stability of polyphenolics throughout storage is important. We determined the stability of polyphenolics in five products (ice pop, oatmeal bar, graham cracker cookie, juice, and gummy product) prepared with WBB powder. Samples stored at 21 °C, 4.4 °C, or −20 °C (ice pops only) were analyzed at 0, 2, 4, 6, and 8 weeks for polyphenolic content and percent polymeric color. Total anthocyanins decreased over storage and storage temperatures in all products. However, the ice pop and the refrigerated juice both retained over 90% of their initial total anthocyanin content. The refrigerated oatmeal bar also showed good retention of anthocyanins (86%), but the gummy product retained only 43% and 51% when stored at 4.4 °C or 21 °C, respectively. The lower amount of polyphenolic compounds recovered in the gummies stored at 4.4 °C compared to 21 °C may be attributed to reduced extraction efficiency as a result of gel hardening at refrigerated temperature. Chlorogenic acid and flavonols were generally more stable than anthocyanins throughout storage.

scholarly journals Evaluation of Anthocyanin Profiles in Various Blackcurrant Cultivars over a Three-Year Period Using a Fast HPLC-DAD Method

Foods ◽  
2021 ◽  
Vol 10 (8) ◽  
pp. 1745
Author(s):  
Barbora Šimerdová ◽  
Michaela Bobríková ◽  
Ivona Lhotská ◽  
Jiří Kaplan ◽  
Alena Křenová ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Health Benefits ◽  
Anthocyanin Content ◽  
Total Anthocyanin ◽  
Similar Profile ◽  
Total Anthocyanin Content ◽  
Total Anthocyanins ◽  
High Antioxidant Activity ◽  
The Individual

Anthocyanins are the most important polyphenolic substances contained in blackcurrant fruits. They are responsible for the various health benefits caused, in particular, by their high antioxidant activity. Anthocyanins derived from anthocyanidins cyanidin and delphinidin are typical for blackcurrant fruits, especially their rutinoside and glucoside forms. These four anthocyanins usually represent about 97–98% of total anthocyanins in blackcurrant fruits. In this study, we developed and validated a new HPLC-DAD method for rapid anthocyanin separation and determination in fifteen perspective blackcurrant cultivars (‘Ruben’, ‘Ben Lomond’, ‘Ben Conan’, ‘Ceres’, ‘Moravia’, ‘Ometa’, ‘Lota’, ‘Fokus’, ‘Tenah’, ‘Sejanec’, ‘Consort’, ‘Triton’, ‘Ben Hope’, ‘Ben Gairn’, and one gooseberry hybrid ‘Josta’). Eight of them were monitored throughout the three-year experiment. The most represented anthocyanins in all monitored blackcurrant cultivars were delphinidin-3-rutinoside (36.7–63.6%), cyanidin-3-rutinoside (26.4–40.6%), delphinidin-3-glucoside (6.1–17.9%), and cyanidin-3-glucoside (1.3–9.9%). The individual anthocyanin proportion (%) in each cultivar was specific, and a similar profile was verified in a three-year period for eight available cultivars. Total anthocyanin content expressed as a sum of four major anthocyanins present in blackcurrants was compared with values expressed as the equivalent of cyanidin-3-glucoside, as many authors do. We revealed an underestimation of about 20% with the latter method. Cultivars with the highest average total anthocyanin content were ‘Ben Gairn’ (294.38 mg/100 g), ‘Ceres’ (281.31 mg/100 g), and ‘Ometa’ (269.09 mg/100 g).

scholarly journals FRUIT CLUSTER THINNING, SHADIHG, AND DEFOLIATION ON FRUIT COLOR DEVELOPMENT OF RELIANCE GRAPES

HortScience ◽  
1992 ◽  
Vol 27 (6) ◽  
pp. 600a-600 ◽  
Author(s):  
Yu Gao ◽  
G. A. Cahoon
Keyword(s):  
High Performance ◽  
Fruit Color ◽  
Soluble Carbohydrates ◽  
Anthocyanin Content ◽  
Total Anthocyanin ◽  
Color Development ◽  
Shade Cloth ◽  
Anthocyanin Profile ◽  
Total Anthocyanin Content ◽  
Cluster Thinning

Significant differences in fruit color were created with fruit cluster thinning (20, 40, and 60 clusters/vine), cluster shading (full sun as control, 55% shading, and 95% shading using shading cage constructed of shade cloth), and defoliation (3, 6, 9, 12, and 15 leaves/cluster). Fruit cluster shading and defoliation treatments decreased red fruit color (characterized by Hunte Color a). Fruit cluster thinning increased red fruit color. Anthocyanin profile of Reliance grape was characterized as cyaninidin-3-glucoside and delphinidin-3-glucoside using Paper Chromatography and Thin Layer Chromatography. Analyses of total anthocyanin content (pH shift method), individual anthocyanin and soluble carbohydrates content (High Performance Liquid Chromatography), are being conducted to determine effects of carbohydrate allocation to fruit and sun light on fruit color of Reliance grapes.

scholarly journals Compound Identification of Selected Rose Species and Cultivars: an Insight to Petal and Leaf Phenolic Profiles

2014 ◽  
Vol 139 (2) ◽  
pp. 157-166 ◽  
Author(s):  
Vlasta Cunja ◽  
Maja Mikulic-Petkovsek ◽  
Franci Stampar ◽  
Valentina Schmitzer
Keyword(s):  
Phenolic Compounds ◽  
High Performance ◽  
Anthocyanin Content ◽  
Compound Identification ◽  
Multivariate Statistical ◽  
Total Anthocyanin ◽  
Total Anthocyanin Content ◽  
Phenolic Constituents ◽  
Phenolic Profiles ◽  
Low Levels

Using high-performance liquid chromatography/mass spectrometry, leaf and petal phenolic profiles of four rose (Rosa) species (R. canina, R. glauca, R. rubiginosa, R. sempervirens) traditionally used for medicinal purposes and three modern rose cultivars (Rosarium Uetersen, Ulrich Brunner Fils, Schwanensee) were determined. An abundance of phenolic constituents was identified: seven different anthocyanins and 31 flavonols in petals; 30 flavonols, 14 phenolic acids, and their derivatives; 15 flavanols; and 20 hydrolysable tannins in leaves. Additionally, petal color was measured with a colorimeter and regression analysis indicated a strong correlation between color parameter a* and total anthocyanin content. The content and composition of phenolic compounds varied significantly among species and cultivars and plant organs investigated. Distinct differences in the distribution of leaf phenolic compounds were observed, especially between Rosa species and modern rose cultivars. In general, leaves of analyzed species were richer in content of most phenolic groups and individual components compared with cultivars. Multivariate statistical analysis clustered the investigated species and cultivars into three distinct groups. Among species, leaves of R. canina stood out with their high and varied phenolic content. Conversely, leaves of the susceptible cultivar Schwanensee appeared most dissimilar as a result of their low levels of phenolic constituents.

scholarly journals Growth and Pigmentation in Three Poinsettia Cultivars

HortScience ◽  
2004 ◽  
Vol 39 (4) ◽  
pp. 837A-837
Author(s):  
Alejandro Garcia ◽  
María Teresa Colinas-León ◽  
Iran Alia-Tejacal* ◽  
Jaime Sahagún-Castellanos
Keyword(s):  
Leaf Area ◽  
Specific Weight ◽  
Anthocyanin Content ◽  
Specific Leaf Weight ◽  
Total Anthocyanin ◽  
Growing Period ◽  
Average Temperature ◽  
Total Anthocyanin Content ◽  
Total Anthocyanins ◽  
Growing Conditions

Marmol, Subjibi, and V-17 Angelika cultivars were evaluated under two different growing conditions; Cuautla (average temperature of 24.6 °C, 70% to 80% relative humidity (RH), latitude 18°49', altitude 1,300 msl) and Texcoco (average temperature of 18 °C, 40% to 50% RH, latitude 19°23'40”, altitude 2,250 msl), under greenhouse conditions in 6” pots. Variables considered were: total anthocyanins, carotenoids and chlorophyll content, chlorophyll a, b, and a/b, specific weight and leaf area, to evaluate differences between growing conditions. There were significative differences in total anthocyanin content for `Subjibi' and V-17 `Angelika', being higher in plants grown in Texcoco. In relation to carotenoids and chlorophylls there were differences for plants from the two growing regions, being Cuautla (highest temperature) where these pigments were higher independent of the cultivar. Specific leaf weight was higher for plants grown in Texcoco with significant differences for `Subjibi' and V-17 `Angelika'. Leaf area was higher for the three cultivars in Cuautla throughout the growing period.

CHANGES IN THE CHEMICAL COMPOSITION OF CONCORD GRAPES GROWN IN ONTARIO DURING RIPENING IN THE 1970 SEASON

1972 ◽  
Vol 52 (6) ◽  
pp. 863-868 ◽  
Author(s):  
TIBOR FULEKI
Keyword(s):  
Chemical Composition ◽  
Titratable Acidity ◽  
The Other ◽  
Soluble Solids ◽  
Anthocyanin Content ◽  
Methyl Anthranilate ◽  
Total Anthocyanin ◽  
Total Anthocyanin Content ◽  
Total Anthocyanins ◽  
Concord Grapes

Methyl anthranilate, soluble solids, titratable acidity, pH, and total anthocyanin content of Concord grapes was followed during ripening in the 1970 season. Methyl anthranilate content increased from 0.04 ppm on 27 August to 6.44 ppm on 26 October and declined thereafter. The maximum values for the other components examined were: total anthocyanins, 114 mg/100 g (30 Oct.); soluble solids, 16.4% (13 Oct.); titratable acidity (minimum), 9.45‰ (16 Oct.); and sugar/acid, 16.93 (16 Oct.).

Sign in / Sign up

Export Citation Format

Share Document