scholarly journals FRUIT CLUSTER THINNING, SHADIHG, AND DEFOLIATION ON FRUIT COLOR DEVELOPMENT OF RELIANCE GRAPES

HortScience ◽  
1992 ◽  
Vol 27 (6) ◽  
pp. 600a-600 ◽  
Author(s):  
Yu Gao ◽  
G. A. Cahoon
Keyword(s):  
High Performance ◽  
Fruit Color ◽  
Soluble Carbohydrates ◽  
Anthocyanin Content ◽  
Total Anthocyanin ◽  
Color Development ◽  
Shade Cloth ◽  
Anthocyanin Profile ◽  
Total Anthocyanin Content ◽  
Cluster Thinning

Significant differences in fruit color were created with fruit cluster thinning (20, 40, and 60 clusters/vine), cluster shading (full sun as control, 55% shading, and 95% shading using shading cage constructed of shade cloth), and defoliation (3, 6, 9, 12, and 15 leaves/cluster). Fruit cluster shading and defoliation treatments decreased red fruit color (characterized by Hunte Color a). Fruit cluster thinning increased red fruit color. Anthocyanin profile of Reliance grape was characterized as cyaninidin-3-glucoside and delphinidin-3-glucoside using Paper Chromatography and Thin Layer Chromatography. Analyses of total anthocyanin content (pH shift method), individual anthocyanin and soluble carbohydrates content (High Performance Liquid Chromatography), are being conducted to determine effects of carbohydrate allocation to fruit and sun light on fruit color of Reliance grapes.

scholarly journals Linking anthocyanin diversity, hue, and genetics in purple corn

2021 ◽  
Vol 11 (2) ◽  
Author(s):  
Laura A Chatham ◽  
John A Juvik
Keyword(s):  
Genotyping By Sequencing ◽  
Future Research ◽  
Anthocyanin Content ◽  
Total Anthocyanin ◽  
Natural Colorant ◽  
Anthocyanin Profile ◽  
Total Anthocyanin Content ◽  
Maize Varieties ◽  
Branching Points ◽  
Purple Corn

AbstractWhile maize with anthocyanin-rich pericarp (purple corn) is rising in popularity as a source of natural colorant for foods and beverages, information on color range and stability—factors associated with anthocyanin decorations and compositional profiles—is currently limited. Furthermore, to maximize the scalability and meet growing demands, both anthocyanin concentrations and agronomic performance must improve in purple corn varieties. Using the natural anthocyanin diversity present in a purple corn landrace, Apache Red, we generated a population with variable flavonoid profiles—flavanol–anthocyanin condensed forms (0–83%), acylated anthocyanins (2–72%), pelargonidin-derived anthocyanins (5–99%), C-glycosyl flavone co-pigments up to 1904 µg/g, and with anthocyanin content up to 1598 µg/g. Each aspect of the flavonoid profiles was found to play a role in either the resulting extract hue or intensity. With genotyping-by-sequencing of this population, we mapped aspects of the flavonoid profile. Major quantitative trait loci (QTLs) for anthocyanin type were found near loci previously identified only in aleurone-pigmented maize varieties [Purple aleurone1 (Pr1) and Anthocyanin acyltransferase1 (Aat1)]. A QTL near P1 (Pericarp color1) was found for both flavone content and flavanol–anthocyanin condensed forms. A significant QTL associated with peonidin-derived anthocyanins near a candidate S-adenosylmethionine-dependent methyltransferase was also identified, warranting further investigation. Mapping total anthocyanin content produced signals near Aat1, the aleurone-associated bHLH R1 (Colored1), the plant color-associated MYB, Pl1 (Purple plant1), the aleurone-associated recessive intensifier, In1 (Intensifier1), and several previously unidentified candidates. This population represents one of the most anthocyanin diverse pericarp-pigmented maize varieties characterized to date. Moreover, the candidates identified here will serve as branching points for future research studying the genetic and molecular processes determining anthocyanin profile in pericarp.

scholarly journals Detection of Copper (II) and Iron (III) in Aqueous Solutions Using the Spectroscopic Characteristics of Bugnay (Antidesma bunius) Anthocyanins

2014 ◽  
pp. 102-118
Author(s):  
Jonathan Barcelo ◽  
Jan Narlo Abril Abril ◽  
Khristine Mereille Castillo ◽  
Alloisa Diaz ◽  
Jonathan Paul Ladera ◽  
...  
Keyword(s):  
High Performance ◽  
Solid Phase ◽  
Copper Ions ◽  
Reversed Phase ◽  
Differential Method ◽  
Anthocyanin Content ◽  
Total Anthocyanin ◽  
Acidic Solutions ◽  
Total Anthocyanin Content ◽  
Spectroscopic Characteristics

The spectrophotometric characteristics of bugnay (Antidesma bunius) anthocyanins in acidified solutions of copper (Cu2+) and iron (Fe3+) were investigated after one hour of reaction to determine the changes in their absorbance characteristics. Anthocyanins from bugnay were isolated using solid phase extraction followed by evaporation at 40°C. The total anthocyanin content of the extract was determined to be 103.87 ± 2.91 mg/L cyanidin-3O-glucoside equivalents using pH differential method. Maximum absorbance readings at pH 1.0 and 4.5 were determined to be at 520 nm and 350 nm, respectively. Cyanidin-3-glucoside was identified as one of the components of the three pigments in the extract using reversed phase high performance liquid chromatography. At pH 1.0, copper caused greater hypochromic shift of bugnai anthocyanins compared to iron (p<0.01) while iron caused greater hypochromic shift at pH 4.5. Copper also caused hypsochromic shift of anthocyanins from 520nm to 350nm at pH 1.0 but not at pH 4.5. Correlation analysis showed a significant moderate positive correlation between mean % hypochromic shift and concentration of copper ions at pH 1.0 (R2 = 0.603, ρ<0.01) and 4.5 (R2 = 0.533, ρ<0.01), and iron at pH 4.5 (R2 = 0.638, ρ<0.01). The spectroscopic characteristics of bugnay anthocyanins at 350 nm and 520 nm can be used as parameters to detect copper and iron in acidic solutions.

scholarly journals Compound Identification of Selected Rose Species and Cultivars: an Insight to Petal and Leaf Phenolic Profiles

2014 ◽  
Vol 139 (2) ◽  
pp. 157-166 ◽  
Author(s):  
Vlasta Cunja ◽  
Maja Mikulic-Petkovsek ◽  
Franci Stampar ◽  
Valentina Schmitzer
Keyword(s):  
Phenolic Compounds ◽  
High Performance ◽  
Anthocyanin Content ◽  
Compound Identification ◽  
Multivariate Statistical ◽  
Total Anthocyanin ◽  
Total Anthocyanin Content ◽  
Phenolic Constituents ◽  
Phenolic Profiles ◽  
Low Levels

Using high-performance liquid chromatography/mass spectrometry, leaf and petal phenolic profiles of four rose (Rosa) species (R. canina, R. glauca, R. rubiginosa, R. sempervirens) traditionally used for medicinal purposes and three modern rose cultivars (Rosarium Uetersen, Ulrich Brunner Fils, Schwanensee) were determined. An abundance of phenolic constituents was identified: seven different anthocyanins and 31 flavonols in petals; 30 flavonols, 14 phenolic acids, and their derivatives; 15 flavanols; and 20 hydrolysable tannins in leaves. Additionally, petal color was measured with a colorimeter and regression analysis indicated a strong correlation between color parameter a* and total anthocyanin content. The content and composition of phenolic compounds varied significantly among species and cultivars and plant organs investigated. Distinct differences in the distribution of leaf phenolic compounds were observed, especially between Rosa species and modern rose cultivars. In general, leaves of analyzed species were richer in content of most phenolic groups and individual components compared with cultivars. Multivariate statistical analysis clustered the investigated species and cultivars into three distinct groups. Among species, leaves of R. canina stood out with their high and varied phenolic content. Conversely, leaves of the susceptible cultivar Schwanensee appeared most dissimilar as a result of their low levels of phenolic constituents.

scholarly journals Study of diversity of anthocyanin composition in bilberry (Vaccinium myrtillus L.) fruits

Medicina ◽  
2007 ◽  
Vol 43 (12) ◽  
pp. 971 ◽  
Author(s):  
Deividas Burdulis ◽  
Liudas Ivanauskas ◽  
Vidmantas Dirsė ◽  
Saulius Kazlauskas ◽  
Almantas Ražukas
Keyword(s):  
High Performance ◽  
Qualitative Evaluation ◽  
Vaccinium Myrtillus ◽  
Quantitative Composition ◽  
Anthocyanin Content ◽  
Total Anthocyanin ◽  
Total Anthocyanin Content ◽  
Total Anthocyanins ◽  
Hydrolysis Of ◽  
Vaccinium Myrtillus L

Qualitative and quantitative composition of anthocyanins in bilberry (Vaccinium myrtillus L.) fruits was assayed. The aim of our study was to evaluate total anthocyanin content and their composition in bilberries collected from various regions and at different time. For the quantification of total anthocyanins in frozen fruits, the spectrophotometrical assay was performed. The highest amount of anthocyanins in bilberry fruits, collected in Lithuania, was found in samples from Šilutė (0.399%), the lowest one – from Valkininkai region (0.264%), but higher amounts of anthocyanins were found in the samples collected in Russia (Archangelsk region) and Sweden (Stockholm region). High-performance liquid chromatography was applied for qualitative evaluation of individual anthocyanins in the different material. Quantification of anthocyanidin content was performed after acidic hydrolysis of anthocyanin glycosides. Chromatographic analysis has shown that there are no differences in qualitative composition of anthocyanidins. In all samples, cyanidin was found in the highest quantities (mean amount 0.053 µg/mL). Delphinidin and petunidin was found in quantities 2.5 fold lower than cyanidin, and malvidin and peonidin were found in the smallest quantities. Only in the blueberries collected in Sweden, malvidin was found in the highest amount. It was 1.5 fold higher than amounts of petunidin and delphinidin.

Inhibitory effects of bioaccessible anthocyanins and procyanidins from apple, red grape, cinnamon on α-amylase, α-glucosidase and lipase

2020 ◽  
pp. 1-9
Author(s):  
Pınar Ercan ◽  
Sedef Nehir El
Keyword(s):  
Inhibitory Activity ◽  
Digestive Enzymes ◽  
Positive Control ◽  
Anthocyanin Content ◽  
Inhibitory Effects ◽  
Total Anthocyanin ◽  
Total Anthocyanin Content ◽  
Before And After ◽  
Red Grape

Abstract. The goals of this study were to determine and evaluate the bioaccessibility of total anthocyanin and procyanidin in apple (Amasya, Malus communis), red grape (Papazkarası, Vitis vinifera) and cinnamon (Cassia, Cinnamomum) using an in vitro static digestion system based on human gastrointestinal physiologically relevant conditions. Also, in vitro inhibitory effects of these foods on lipid (lipase) and carbohydrate digestive enzymes (α-amylase and α-glucosidase) were performed with before and after digested samples using acarbose and methylumbelliferyl oleate (4MUO) as the positive control. While the highest total anthocyanin content was found in red grape (164 ± 2.51 mg/100 g), the highest procyanidin content was found in cinnamon (6432 ± 177.31 mg/100 g) (p < 0.05). The anthocyanin bioaccessibilities were found as 10.2 ± 1%, 8.23 ± 0.64%, and 8.73 ± 0.70% in apple, red grape, and cinnamon, respectively. The procyanidin bioaccessibilities of apple, red grape, and cinnamon were found as 17.57 ± 0.71%, 14.08 ± 0.74% and 18.75 ± 1.49%, respectively. The analyzed apple, red grape and cinnamon showed the inhibitory activity against α-glucosidase (IC50 544 ± 21.94, 445 ± 15.67, 1592 ± 17.58 μg/mL, respectively), α-amylase (IC50 38.4 ± 7.26, 56.1 ± 3.60, 3.54 ± 0.86 μg/mL, respectively), and lipase (IC50 52.7 ± 2.05, 581 ± 54.14, 49.6 ± 2.72 μg/mL), respectively. According to our results apple, red grape and cinnamon have potential to inhibit of lipase, α-amylase and α-glucosidase digestive enzymes.

scholarly journals Effect of Freeze Crystallization on Quality Properties of Two Endemic Patagonian Berries Juices: Murta (Ugni molinae) and Arrayan (Luma apiculata)

Foods ◽  
2021 ◽  
Vol 10 (2) ◽  
pp. 466
Author(s):  
María Guerra-Valle ◽  
Siegried Lillo-Perez ◽  
Guillermo Petzold ◽  
Patricio Orellana-Palma
Keyword(s):  
Process Parameters ◽  
Soluble Solids ◽  
Total Phenolic ◽  
Anthocyanin Content ◽  
Flavonoid Content ◽  
Total Polyphenol ◽  
Total Anthocyanin ◽  
Quality Properties ◽  
Total Anthocyanin Content ◽  
Ugni Molinae

This work studied the effects of centrifugal block freeze crystallization (CBFC) on physicochemical parameters, total phenolic compound content (TPCC), antioxidant activity (AA), and process parameters applied to fresh murta and arrayan juices. In the last cycle, for fresh murta and arrayan juices, the total soluble solids (TSS) showed values close to 48 and 54 Brix, and TPCC exhibited values of approximately 20 and 66 mg gallic acid equivalents/100 grams dry matter (d.m.) for total polyphenol content, 13 and 25 mg cyanidin-3-glucoside equivalents/100 grams d.m. for total anthocyanin content, and 9 and 17 mg quercetin equivalents/100 grams d.m. for total flavonoid content, respectively. Moreover, the TPCC retention indicated values over 78% for murta juice, and 82% for arrayan juice. Similarly, the AA presented an increase over 2.1 times in relation to the correspondent initial AA value. Thus, the process parameters values were between 69% and 85% for efficiency, 70% and 88% for percentage of concentrate, and 0.72% and 0.88 (kg solutes/kg initial solutes) for solute yield. Therefore, this work provides insight about CBFC on valuable properties in fresh Patagonian berries juices, for future applications in health and industrial scale.

scholarly journals Differences in Anthocyanin Accumulation Profiles between Teinturier and Non-Teinturier Cultivars during Ripening

Foods ◽  
2021 ◽  
Vol 10 (5) ◽  
pp. 1073
Author(s):  
Meng-Bo Tian ◽  
Lin Yuan ◽  
Ming-Yuan Zheng ◽  
Zhu-Mei Xi
Keyword(s):  
Gene Expression ◽  
Plant Secondary Metabolites ◽  
Anthocyanin Synthesis ◽  
Anthocyanin Content ◽  
Anthocyanin Accumulation ◽  
Total Anthocyanin ◽  
Total Anthocyanin Content ◽  
The Individual ◽  
Relative Gene ◽  
High Expression Level

Anthocyanins are vital components of plant secondary metabolites, and are also the most important coloring substances in wine. Teinturier cultivars are rich in anthocyanins. However, the differences in anthocyanin accumulation and profiles between teinturier and non-teinturier cultivars have not been reported. In this study, Yan 73 and Dunkelfelder were selected as the experimental materials, and three non-teinturier cultivars were used for comparison. LC-MS and qRT-PCR were used to determine the individual anthocyanin contents and the relative gene expression. The results show that the total anthocyanin content of the teinturier cultivars was considerably higher than that in non-teinturier cultivars, and the levels of individual anthocyanins increased gradually during ripening. Lower ratios of modified anthocyanins were found in the teinturier cultivars, which was not only due to the high expression level of VvUFGT and VvGST4, but also due to the relatively low expression of VvOMT in these cultivars. Cluster analysis of gene expression and anthocyanin accumulation showed that VvUFGT is related to anthocyanin accumulation, and that AM1 is related to the synthesis and transport of methylated anthocyanins. Our results will be useful for further clarifying the pathways of anthocyanin synthesis, modification, and transport in teinturier cultivars.

scholarly journals ANTHOCYANIN AND ANTHOCYANIDIN CONTENT OF HIGHBUSH BLUEBERRIES CULTIVATED IN BRAZIL

2013 ◽  
Vol 31 (2) ◽  
Author(s):  
JÚLIA RIBEIRO SARKIS ◽  
ISABEL CRISTINA TESSARO ◽  
LIGIA DAMASCENO FERREIRA MAECZAK
Keyword(s):  
Gradient Elution ◽  
Vaccinium Corymbosum ◽  
Differential Method ◽  
Anthocyanin Content ◽  
Qualitative And Quantitative Analysis ◽  
Total Anthocyanin ◽  
Qualitative And Quantitative ◽  
Total Anthocyanin Content ◽  
Highbush Blueberries ◽  
Fresh Pulp

The objective of this study was to apply a HPLC methodology for qualitative and quantitative analysis of the six anthocyanidins present in fruits and to analyze the anthocyanin and anthocyanidin content of blueberries cultivated in Southern Brazil. The samples used belong to highbush blueberries (Vaccinium corymbosum) cultivars. Total anthocyanin content was determined by the pH differential method and an HPLC gradient elution system with C18 column and UV-Vis detection at 520 nm were used for separation and quantification of the anthocyanidins. Total anthocyanin content was of 128 ± 3 mg per 100 g of fresh pulp. Blueberry pulp presented 55 % of delphinidin, 8 % of cyanidin, 3 % of peonidin and 34 % of malvidin. Pelargonidin was not identified in the sample and petunidin was below the limits of quantification. The results were similar to those reported in studies using North American and European blueberries.

scholarly journals Transcriptome analyses reveal anthocyanin biosynthesis in eggplants

2018 ◽  
Author(s):  
Xi Ou Xiao ◽  
Wen qiu Lin ◽  
Ke Li ◽  
Xue Feng Feng ◽  
Hui Jin ◽  
...  
Keyword(s):  
Anthocyanin Biosynthesis ◽  
Negative Regulation ◽  
Anthocyanin Content ◽  
Biosynthesis Pathway ◽  
Rna Seq ◽  
Wild Type ◽  
Rt Pcr ◽  
Total Anthocyanin ◽  
Total Anthocyanin Content ◽  
Cumulative Knowledge

We obtained a white-peel eggplant (L6-5) by EMS mutation in our previous study, whose total anthocyanin content was significantly decreased as compared with that of wild-type (WT). To analyse the anthocyanin biosynthesis mechanism in eggplants, we analysed the eggplant peel by RNA-seq in this study. The transcript results revealed upregulation of 465 genes and downregulation of 525 genes in L6-5 as compared with the WT eggplant. A total of 11 anthocyanin biosynthesis structure genes were significantly downregulated in L6-5 as compared with that in WT. Meanwhile, on the basis of the RT-PCR results of four natural eggplant cultivars, the expression pattern of 11 anthocyanin biosynthesis structure genes was consistent with the anthocyanin content. Thus, we speculated the anthocyanin biosynthesis pathway in eggplant peel. The transcript and RT-PCR results suggested positive regulation of MYB1, MYB108 and TTG8 and negative regulation of bHLH36 in anthocyanin biosynthesis. This study enhanced our cumulative knowledge about anthocyanin biosynthesis in eggplant peels.

scholarly journals Effect of drying techniques on the retention of antioxidant activities of Saskatoon berries

2013 ◽  
Vol 2 (2) ◽  
Author(s):  
Pranabendu Mitra ◽  
Venkatesh Meda ◽  
Rick Green
Keyword(s):  
Antioxidant Activity ◽  
Radical Scavenging ◽  
Vacuum Drying ◽  
Anthocyanin Content ◽  
Dpph Radical ◽  
Total Anthocyanin ◽  
Hot Air ◽  
Total Anthocyanin Content ◽  
Dpph Radical Scavenging ◽  
Radical Scavenging Assay

The main objective of this research was to compare the retention of antioxidant activity and total anthocyanin content of Saskatoon berries dried by freeze drying, microwave-vacuum drying, thin layer hot air drying and vacuum drying. Antioxidant activity of berry samples was determined by DPPH radical scavenging and ABTS radical scavenging, and the pH differential method was used to determine total anthocyanin content of the berry samples. The results showed that the freeze dried Saskatoon berries exhibited the highest retention of anthocyanin and antioxidant activity among the dried samples, followed by microwave-vacuum dried berries, thin layer hot air dried berries and vacuum dried berries. There were significant differences between the berry samples at P<0.05.  DPPH radical scavenging and ABTS radical scavenging were correlated linearly with an R2 value of 0.99 at P<0.05 showing their effectiveness for the determination of the antioxidant activity of the Saskatoon berries. However, the DPPH radical scavenging assay was more effective than the ABTS radical scavenging assay. The results also showed that antioxidant activity of the berries was highly correlated with the total anthocyanin content of the fruit. The reduction of anthocyanin in dried berry samples was linearly correlated with the reduction of DPPH radical scavenging with an R2 value of 0.97 at P<0.05 and, also, linearly correlated with the reduction of ABTS radical scavenging with an R2 value of 0.88 at P<0.05.

Sign in / Sign up

Export Citation Format

Share Document