scholarly journals Detection of Copper (II) and Iron (III) in Aqueous Solutions Using the Spectroscopic Characteristics of Bugnay (Antidesma bunius) Anthocyanins

2014 ◽  
pp. 102-118
Author(s):  
Jonathan Barcelo ◽  
Jan Narlo Abril Abril ◽  
Khristine Mereille Castillo ◽  
Alloisa Diaz ◽  
Jonathan Paul Ladera ◽  
...  
Keyword(s):  
High Performance ◽  
Solid Phase ◽  
Copper Ions ◽  
Reversed Phase ◽  
Differential Method ◽  
Anthocyanin Content ◽  
Total Anthocyanin ◽  
Acidic Solutions ◽  
Total Anthocyanin Content ◽  
Spectroscopic Characteristics

The spectrophotometric characteristics of bugnay (Antidesma bunius) anthocyanins in acidified solutions of copper (Cu2+) and iron (Fe3+) were investigated after one hour of reaction to determine the changes in their absorbance characteristics. Anthocyanins from bugnay were isolated using solid phase extraction followed by evaporation at 40°C. The total anthocyanin content of the extract was determined to be 103.87 ± 2.91 mg/L cyanidin-3O-glucoside equivalents using pH differential method. Maximum absorbance readings at pH 1.0 and 4.5 were determined to be at 520 nm and 350 nm, respectively. Cyanidin-3-glucoside was identified as one of the components of the three pigments in the extract using reversed phase high performance liquid chromatography. At pH 1.0, copper caused greater hypochromic shift of bugnai anthocyanins compared to iron (p<0.01) while iron caused greater hypochromic shift at pH 4.5. Copper also caused hypsochromic shift of anthocyanins from 520nm to 350nm at pH 1.0 but not at pH 4.5. Correlation analysis showed a significant moderate positive correlation between mean % hypochromic shift and concentration of copper ions at pH 1.0 (R2 = 0.603, ρ<0.01) and 4.5 (R2 = 0.533, ρ<0.01), and iron at pH 4.5 (R2 = 0.638, ρ<0.01). The spectroscopic characteristics of bugnay anthocyanins at 350 nm and 520 nm can be used as parameters to detect copper and iron in acidic solutions.

scholarly journals ANTHOCYANIN AND ANTHOCYANIDIN CONTENT OF HIGHBUSH BLUEBERRIES CULTIVATED IN BRAZIL

2013 ◽  
Vol 31 (2) ◽  
Author(s):  
JÚLIA RIBEIRO SARKIS ◽  
ISABEL CRISTINA TESSARO ◽  
LIGIA DAMASCENO FERREIRA MAECZAK
Keyword(s):  
Gradient Elution ◽  
Vaccinium Corymbosum ◽  
Differential Method ◽  
Anthocyanin Content ◽  
Qualitative And Quantitative Analysis ◽  
Total Anthocyanin ◽  
Qualitative And Quantitative ◽  
Total Anthocyanin Content ◽  
Highbush Blueberries ◽  
Fresh Pulp

The objective of this study was to apply a HPLC methodology for qualitative and quantitative analysis of the six anthocyanidins present in fruits and to analyze the anthocyanin and anthocyanidin content of blueberries cultivated in Southern Brazil. The samples used belong to highbush blueberries (Vaccinium corymbosum) cultivars. Total anthocyanin content was determined by the pH differential method and an HPLC gradient elution system with C18 column and UV-Vis detection at 520 nm were used for separation and quantification of the anthocyanidins. Total anthocyanin content was of 128 ± 3 mg per 100 g of fresh pulp. Blueberry pulp presented 55 % of delphinidin, 8 % of cyanidin, 3 % of peonidin and 34 % of malvidin. Pelargonidin was not identified in the sample and petunidin was below the limits of quantification. The results were similar to those reported in studies using North American and European blueberries.

scholarly journals Isolation and free-radical-scavenging properties of cyanidin 3-O-glycosides from the fruits of Ribes biebersteinii Berl.

2010 ◽  
Vol 60 (1) ◽  
pp. 1-11 ◽  
Author(s):  
Abbas Delazar ◽  
Laleh Khodaie ◽  
Jalil Afshar ◽  
Lutfun Nahar ◽  
Satyajit Sarker
Keyword(s):  
Free Radical ◽  
High Performance ◽  
Radical Scavenging ◽  
High Performance Liquid Chromatographic ◽  
Reversed Phase ◽  
Free Radical Scavenging ◽  
Anthocyanin Content ◽  
Total Anthocyanin ◽  
Radical Scavenging Properties ◽  
Liquid Chromatographic

Isolation and free-radical-scavenging properties of cyanidin 3-O-glycosides from the fruits of Ribes biebersteinii Berl. The reversed-phase preparative high performance liquid chromatographic purification of the methanol extract of the fruits of Ribes biebersteinii Berl. (Grossulariaceae) afforded five cyanidin glycosides, 3-O-sambubiosyl-5-O-glucosyl cyanidin (1), cyanidin 3-O-sambubioside (2), cyanidin 3-O-glucoside (3), cyanidin 3-O-(2G-xylosyl)-rutinoside (4) and cyanidin 3-O-rutinoside (5). They showed considerable free-radical-scavenging properties in the 2,2-diphenyl-1-picrylhydrazyl (DPPH) assay with the RC50 values of 9.29 × 10-6, 9.33 × 10-6, 8.31 × 10-6, 8.96 × 10-6 and 9.55 × 10-6 mol L-1, respectively. The structures of these compounds were elucidated by various chemical hydrolyses and spectroscopic means. The total anthocyanin content was 1.9 g per 100 g dried fruits on cyanidin 3-glucoside basis.

scholarly journals Effect of Light on Anthocyanin Levels in Submerged, Harvested Cranberry Fruit

2004 ◽  
Vol 2004 (5) ◽  
pp. 259-263 ◽  
Author(s):  
Yu Zhou ◽  
Bal Ram Singh
Keyword(s):  
High Performance ◽  
Anthocyanin Biosynthesis ◽  
Water Immersion ◽  
Red Light ◽  
Control Sample ◽  
Reversed Phase ◽  
Hplc Method ◽  
Natural Light ◽  
Anthocyanin Content ◽  
Total Anthocyanin

Anthocyanins are a group of plant antioxidants known for their therapeutic use. The effects of natural light, red light, and far-red light on individual as well as total anthocyanin content in cranberry fruit (Vaccinium macrocarponAit) were examined in an experimental setting designed to mimic water-harvesting conditions. The reversed-phase high performance liquid chromatography (HPLC) method was used to separate and analyze the anthocyanins. In contrast to the case of the control sample that was kept in the dark, natural light increased the total anthocyanin level by75.3% and87.2% after 24 and 48 hours water immersion, respectively. Red light and far-red light increased the total anthocyanin level by41.5% and34.7%, respectively. The amount of each individual anthocyanin increased differently under natural light, red light, and far-red light, suggesting that expressions of enzymes that catalyze the anthocyanin biosynthesis are regulated differently by environments.

scholarly journals FRUIT CLUSTER THINNING, SHADIHG, AND DEFOLIATION ON FRUIT COLOR DEVELOPMENT OF RELIANCE GRAPES

HortScience ◽  
1992 ◽  
Vol 27 (6) ◽  
pp. 600a-600 ◽  
Author(s):  
Yu Gao ◽  
G. A. Cahoon
Keyword(s):  
High Performance ◽  
Fruit Color ◽  
Soluble Carbohydrates ◽  
Anthocyanin Content ◽  
Total Anthocyanin ◽  
Color Development ◽  
Shade Cloth ◽  
Anthocyanin Profile ◽  
Total Anthocyanin Content ◽  
Cluster Thinning

Significant differences in fruit color were created with fruit cluster thinning (20, 40, and 60 clusters/vine), cluster shading (full sun as control, 55% shading, and 95% shading using shading cage constructed of shade cloth), and defoliation (3, 6, 9, 12, and 15 leaves/cluster). Fruit cluster shading and defoliation treatments decreased red fruit color (characterized by Hunte Color a). Fruit cluster thinning increased red fruit color. Anthocyanin profile of Reliance grape was characterized as cyaninidin-3-glucoside and delphinidin-3-glucoside using Paper Chromatography and Thin Layer Chromatography. Analyses of total anthocyanin content (pH shift method), individual anthocyanin and soluble carbohydrates content (High Performance Liquid Chromatography), are being conducted to determine effects of carbohydrate allocation to fruit and sun light on fruit color of Reliance grapes.

scholarly journals Compound Identification of Selected Rose Species and Cultivars: an Insight to Petal and Leaf Phenolic Profiles

2014 ◽  
Vol 139 (2) ◽  
pp. 157-166 ◽  
Author(s):  
Vlasta Cunja ◽  
Maja Mikulic-Petkovsek ◽  
Franci Stampar ◽  
Valentina Schmitzer
Keyword(s):  
Phenolic Compounds ◽  
High Performance ◽  
Anthocyanin Content ◽  
Compound Identification ◽  
Multivariate Statistical ◽  
Total Anthocyanin ◽  
Total Anthocyanin Content ◽  
Phenolic Constituents ◽  
Phenolic Profiles ◽  
Low Levels

Using high-performance liquid chromatography/mass spectrometry, leaf and petal phenolic profiles of four rose (Rosa) species (R. canina, R. glauca, R. rubiginosa, R. sempervirens) traditionally used for medicinal purposes and three modern rose cultivars (Rosarium Uetersen, Ulrich Brunner Fils, Schwanensee) were determined. An abundance of phenolic constituents was identified: seven different anthocyanins and 31 flavonols in petals; 30 flavonols, 14 phenolic acids, and their derivatives; 15 flavanols; and 20 hydrolysable tannins in leaves. Additionally, petal color was measured with a colorimeter and regression analysis indicated a strong correlation between color parameter a* and total anthocyanin content. The content and composition of phenolic compounds varied significantly among species and cultivars and plant organs investigated. Distinct differences in the distribution of leaf phenolic compounds were observed, especially between Rosa species and modern rose cultivars. In general, leaves of analyzed species were richer in content of most phenolic groups and individual components compared with cultivars. Multivariate statistical analysis clustered the investigated species and cultivars into three distinct groups. Among species, leaves of R. canina stood out with their high and varied phenolic content. Conversely, leaves of the susceptible cultivar Schwanensee appeared most dissimilar as a result of their low levels of phenolic constituents.

scholarly journals Near-Infrared Spectroscopy as a Rapid Screening Method for the Determination of Total Anthocyanin Content in Sambucus Fructus

Sensors ◽  
2020 ◽  
Vol 20 (17) ◽  
pp. 4983
Author(s):  
Stefan Stuppner ◽  
Sophia Mayr ◽  
Anel Beganovic ◽  
Krzysztof Beć ◽  
Justyna Grabska ◽  
...  
Keyword(s):  
Sample Preparation ◽  
Near Infrared ◽  
Screening Method ◽  
Differential Method ◽  
Anthocyanin Content ◽  
Cyanogenic Glycosides ◽  
Total Anthocyanin ◽  
Total Anthocyanin Content ◽  
Tof Ms

Elderberry (Sambucus nigra L., fructus) is a very potent herbal drug, deriving from traditional European medicine (TEM). Ripe elderberries are rich in anthocyanins, flavonols, flavonol esters, flavonol glycosides, lectins, essential oils, unsaturated fatty acids and vitamins. Nevertheless, unripe elderflower fruits contain a certain amount of sambunigrin, a toxic cyanogenic glycoside, whose concentration decreases in the ripening process. Therefore, quality assurance must be carried out. The standard method described in literature is the photometric determination (pH-differential method) of the total anthocyanin content (TAC) that is the highest when the berries are ripe. The drawback of the pH-differential method is the extensive sample preparation and the low accuracy of the method. Therefore, the goal of this publication was to develop a fast non invasive near-infrared (NIR) method for the determination of TAC in whole berries. TAC of elderberries was measured using pH-differentiation method where TAC values of 632.87 mg/kg to 4342.01 mg/kg were measured. Additionally, cyanidin-3-O-glucoside, cyanidin-3-O-sambubioside and cyanidin-3-O-sambubioside-5-O-glucoside which represent more than 98% of TAC in elderberry were quantified using ultra high performance liquid chromatography-multiple wavelength detection—ultra high resolution-quadrupole-time of flight-mass spectrometry (UHPLC-MWD-UHR-Q-TOF-MS) and their sum parameter was determined, ranging between 499.43 mg/kg and 8199.07 mg/kg. Using those two methods as reference, whole elderberries were investigated by NIR spectroscopy with the Büchi NIRFlex N-500 benchtop spectrometer. According to the constructed partial least squares regression (PLSR) models the performance was as follows: a relative standard deviation (RSDPLSR) of 13.5% and root mean square error of calibration (RMSECV/RMSEC) of 1.31 for pH-differentiation reference and a RSDPLSR of 12.9% and RMSECV/RMSEC of 1.28 for the HPLC reference method. In this study, we confirm that it is possible to perform a NIR screening for TAC in whole elderberries. Using quantum chemical calculations, we obtained detailed NIR band assignments of the analyzed compounds and interpreted the wavenumber regions established in PLSR models as meaningful for anthocyanin content. The NIR measurement turned out to be a fast and cost-efficient alternative for the determination of TAC compared to pH-differential method and UHPLC-MWD-UHR-Q-TOF-MS. Due to the benefit of no sample preparation and extraction the technology can be considered as sustainable green technology. With the above mentioned inversely proportional ratio of TAC to total amount of toxic cyanogenic glycosides, NIR proves to be a reliable screening method for the ideal harvest time with maximal content of TAC and lowest content of cyanogenic glycosides in elderberry.

scholarly journals Study of diversity of anthocyanin composition in bilberry (Vaccinium myrtillus L.) fruits

Medicina ◽  
2007 ◽  
Vol 43 (12) ◽  
pp. 971 ◽  
Author(s):  
Deividas Burdulis ◽  
Liudas Ivanauskas ◽  
Vidmantas Dirsė ◽  
Saulius Kazlauskas ◽  
Almantas Ražukas
Keyword(s):  
High Performance ◽  
Qualitative Evaluation ◽  
Vaccinium Myrtillus ◽  
Quantitative Composition ◽  
Anthocyanin Content ◽  
Total Anthocyanin ◽  
Total Anthocyanin Content ◽  
Total Anthocyanins ◽  
Hydrolysis Of ◽  
Vaccinium Myrtillus L

Qualitative and quantitative composition of anthocyanins in bilberry (Vaccinium myrtillus L.) fruits was assayed. The aim of our study was to evaluate total anthocyanin content and their composition in bilberries collected from various regions and at different time. For the quantification of total anthocyanins in frozen fruits, the spectrophotometrical assay was performed. The highest amount of anthocyanins in bilberry fruits, collected in Lithuania, was found in samples from Šilutė (0.399%), the lowest one – from Valkininkai region (0.264%), but higher amounts of anthocyanins were found in the samples collected in Russia (Archangelsk region) and Sweden (Stockholm region). High-performance liquid chromatography was applied for qualitative evaluation of individual anthocyanins in the different material. Quantification of anthocyanidin content was performed after acidic hydrolysis of anthocyanin glycosides. Chromatographic analysis has shown that there are no differences in qualitative composition of anthocyanidins. In all samples, cyanidin was found in the highest quantities (mean amount 0.053 µg/mL). Delphinidin and petunidin was found in quantities 2.5 fold lower than cyanidin, and malvidin and peonidin were found in the smallest quantities. Only in the blueberries collected in Sweden, malvidin was found in the highest amount. It was 1.5 fold higher than amounts of petunidin and delphinidin.

Inhibitory effects of bioaccessible anthocyanins and procyanidins from apple, red grape, cinnamon on α-amylase, α-glucosidase and lipase

2020 ◽  
pp. 1-9
Author(s):  
Pınar Ercan ◽  
Sedef Nehir El
Keyword(s):  
Inhibitory Activity ◽  
Digestive Enzymes ◽  
Positive Control ◽  
Anthocyanin Content ◽  
Inhibitory Effects ◽  
Total Anthocyanin ◽  
Total Anthocyanin Content ◽  
Before And After ◽  
Red Grape

Abstract. The goals of this study were to determine and evaluate the bioaccessibility of total anthocyanin and procyanidin in apple (Amasya, Malus communis), red grape (Papazkarası, Vitis vinifera) and cinnamon (Cassia, Cinnamomum) using an in vitro static digestion system based on human gastrointestinal physiologically relevant conditions. Also, in vitro inhibitory effects of these foods on lipid (lipase) and carbohydrate digestive enzymes (α-amylase and α-glucosidase) were performed with before and after digested samples using acarbose and methylumbelliferyl oleate (4MUO) as the positive control. While the highest total anthocyanin content was found in red grape (164 ± 2.51 mg/100 g), the highest procyanidin content was found in cinnamon (6432 ± 177.31 mg/100 g) (p < 0.05). The anthocyanin bioaccessibilities were found as 10.2 ± 1%, 8.23 ± 0.64%, and 8.73 ± 0.70% in apple, red grape, and cinnamon, respectively. The procyanidin bioaccessibilities of apple, red grape, and cinnamon were found as 17.57 ± 0.71%, 14.08 ± 0.74% and 18.75 ± 1.49%, respectively. The analyzed apple, red grape and cinnamon showed the inhibitory activity against α-glucosidase (IC50 544 ± 21.94, 445 ± 15.67, 1592 ± 17.58 μg/mL, respectively), α-amylase (IC50 38.4 ± 7.26, 56.1 ± 3.60, 3.54 ± 0.86 μg/mL, respectively), and lipase (IC50 52.7 ± 2.05, 581 ± 54.14, 49.6 ± 2.72 μg/mL), respectively. According to our results apple, red grape and cinnamon have potential to inhibit of lipase, α-amylase and α-glucosidase digestive enzymes.

scholarly journals Metabolic Evaluation of Urine from Patients Diagnosed with High Grade (HG) Bladder Cancer by SPME-LC-MS Method

Molecules ◽  
2021 ◽  
Vol 26 (8) ◽  
pp. 2194
Author(s):  
Kamil Łuczykowski ◽  
Natalia Warmuzińska ◽  
Sylwia Operacz ◽  
Iga Stryjak ◽  
Joanna Bogusiewicz ◽  
...  
Keyword(s):  
Thin Film ◽  
Bladder Cancer ◽  
High Performance ◽  
Biomarker Discovery ◽  
Solid Phase ◽  
Biological Fluids ◽  
Reversed Phase ◽  
Control Group ◽  
Metabolic Evaluation ◽  
Metabolomic Profiling

Bladder cancer (BC) is a common malignancy of the urinary system and a leading cause of death worldwide. In this work, untargeted metabolomic profiling of biological fluids is presented as a non-invasive tool for bladder cancer biomarker discovery as a first step towards developing superior methods for detection, treatment, and prevention well as to further our current understanding of this disease. In this study, urine samples from 24 healthy volunteers and 24 BC patients were subjected to metabolomic profiling using high throughput solid-phase microextraction (SPME) in thin-film format and reversed-phase high-performance liquid chromatography coupled with a Q Exactive Focus Orbitrap mass spectrometer. The chemometric analysis enabled the selection of metabolites contributing to the observed separation of BC patients from the control group. Relevant differences were demonstrated for phenylalanine metabolism compounds, i.e., benzoic acid, hippuric acid, and 4-hydroxycinnamic acid. Furthermore, compounds involved in the metabolism of histidine, beta-alanine, and glycerophospholipids were also identified. Thin-film SPME can be efficiently used as an alternative approach to other traditional urine sample preparation methods, demonstrating the SPME technique as a simple and efficient tool for urinary metabolomics research. Moreover, this study’s results may support a better understanding of bladder cancer development and progression mechanisms.

Sign in / Sign up

Export Citation Format

Share Document